breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 135,162 C→A 6.7% intergenic (+209/‑29) EO946_RS00715 → / → rpsJ alpha/beta hydrolase/30S ribosomal protein S10
RA 165,400 C→T 6.6% noncoding (2928/2928 nt) EO946_RS00950 → 23S ribosomal RNA
RA 240,190 G→T 17.1% F114L (TTC→TTA EO946_RS01360 ← hypothetical protein
RA 240,207 G→T 11.2% Q109K (CAA→AAA)  EO946_RS01360 ← hypothetical protein
RA 361,344 T→G 6.8% V144V (GTT→GTG EO946_RS01960 → FAD/NAD(P)‑binding protein
RA 361,366 C→A 8.1% Q152K (CAG→AAG)  EO946_RS01960 → FAD/NAD(P)‑binding protein
RA 452,125 T→A 42.7% K146I (AAA→ATA)  EO946_RS02320 ← dienelactone hydrolase family protein
RA 549,085 C→A 13.1% Q33K (CAA→AAA)  EO946_RS02865 → PLP‑dependent aminotransferase family protein
RA 595,180 G→T 7.0% L22F (TTG→TTT EO946_RS03130 → MMPL family transporter
RA 607,359 A→C 8.9% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,379 G→C 5.7% T74T (ACG→ACC EO946_RS03185 → collagen‑like protein
RA 607,413 A→C 9.5% T86P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,562 G→C 13.4% G135G (GGG→GGC EO946_RS03185 → collagen‑like protein
RA 607,592 A→C 21.1% E145D (GAA→GAC EO946_RS03185 → collagen‑like protein
RA 607,594 C→T 17.7% A146V (GCC→GTC)  EO946_RS03185 → collagen‑like protein
RA 607,638 G→C 10.0% A161P (GCC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,699 A→C 5.3% E181A (GAA→GCA) ‡ EO946_RS03185 → collagen‑like protein
RA 607,700 A→C 12.0% E181D (GAA→GAC) ‡ EO946_RS03185 → collagen‑like protein
RA 634,052 T→G 17.0% E573D (GAA→GAC EO946_RS03330 ← ABC‑F family ATP‑binding cassette domain‑containing protein
RA 755,530 A→C 7.6% Q22P (CAA→CCA) ‡ EO946_RS03880 → rhamnogalacturonan acetylesterase
RA 755,531 A→C 8.2% Q22H (CAA→CAC) ‡ EO946_RS03880 → rhamnogalacturonan acetylesterase
RA 814,356 T→G 13.5% H340Q (CAT→CAG nosA → nitric oxide synthase
RA 847,234 C→A 6.3% C284F (TGT→TTT)  EO946_RS04305 ← NAD(P)‑dependent oxidoreductase
RA 986,786 C→A 10.0% Q367K (CAA→AAA)  spoVR → stage V sporulation protein SpoVR
RA 1,097,430 C→G 8.7% S58C (TCT→TGT)  EO946_RS05675 → MarR family transcriptional regulator
RA 1,097,464 G→T 7.6% T69T (ACG→ACT EO946_RS05675 → MarR family transcriptional regulator
RA 1,112,969 C→T 100% L1022F (CTT→TTT)  addB → helicase‑exonuclease AddAB subunit AddB
RA 1,293,328 G→T 10.7% R134L (CGA→CTA)  EO946_RS06740 → sigma‑70 family RNA polymerase sigma factor
RA 1,720,712 C→A 13.6% T212T (ACC→ACA spoIIIE → DNA translocase SpoIIIE
RA 1,730,080 A→C 6.1% K167N (AAA→AAC rodZ → cell shape determination protein RodZ
RA 1,800,874 A→C 16.4% S3519R (AGC→CGC)  EO946_RS09135 → SDR family NAD(P)‑dependent oxidoreductase
RA 1,936,643 A→C 5.3% D3536E (GAT→GAG) ‡ EO946_RS09735 ← non‑ribosomal peptide synthetase
RA 1,936,644 T→A 9.6% D3536V (GAT→GTT) ‡ EO946_RS09735 ← non‑ribosomal peptide synthetase
RA 2,073,288 A→T 5.5% G335G (GGT→GGA EO946_RS10385 ← NTTRR‑F1 domain
RA 2,073,360 A→T 7.3% G311G (GGT→GGA EO946_RS10385 ← NTTRR‑F1 domain
RA 2,103,772 T→A 8.7% V4V (GTA→GTT azoJ ← FMN‑dependent NADH‑azoreductase AzoJ
RA 2,139,461 C→A 14.7% A126S (GCG→TCG)  deoD ← purine‑nucleoside phosphorylase
RA 2,147,508 T→G 6.3% T173T (ACA→ACC EO946_RS10810 ← CoA transferase subunit A
RA 2,491,058 T→G 6.2% E271D (GAA→GAC floA ← flotillin‑like protein FloA
RA 2,627,197 G→T 6.1% intergenic (‑18/‑115) EO946_RS13595 ← / → EO946_RS13600 YrzK family protein/SH3 domain‑containing protein
RA 2,632,871 A→G 5.4% M575T (ATG→ACG)  recJ ← single‑stranded‑DNA‑specific exonuclease RecJ
RA 2,680,400 A→T 10.5% V417D (GTT→GAT)  EO946_RS13875 ← LysM peptidoglycan‑binding domain‑containing protein
RA 2,812,782 T→G 7.1% K202T (AAG→ACG)  EO946_RS14520 ← SDR family oxidoreductase
RA 2,897,849 A→C 6.5% L106R (CTT→CGT)  EO946_RS14930 ← hypothetical protein
RA 2,978,137 A→T 23.3% noncoding (2664/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 2,979,628 T→A 14.6% noncoding (1173/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 2,980,160 G→A 16.2% noncoding (641/2928 nt) EO946_RS15430 ← 23S ribosomal RNA
RA 2,981,253 C→T 37.7% noncoding (1267/1550 nt) EO946_RS15435 ← 16S ribosomal RNA
RA 2,982,236 T→C 24.2% noncoding (284/1550 nt) EO946_RS15435 ← 16S ribosomal RNA
RA 2,991,252 C→A 22.5% intergenic (+53/‑154) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA
RA 3,111,432 C→A 5.1% intergenic (‑333/+219) EO946_RS16070 ← / ← EO946_RS16080 GNAT family N‑acetyltransferase/iron‑sulfur cluster assembly accessory protein
RA 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
RA 3,268,725 C→A 7.1% intergenic (+73/+137) catR → / ← EO946_RS16920 catDE operon transcriptional regulator CatR/HAMP domain‑containing histidine kinase
RA 3,285,567 C→T 31.7% L278L (CTG→CTA EO946_RS16995 ← IS3 family transposase
RA 3,285,575 T→G 32.1% N276H (AAT→CAT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,580 A→G 35.3% I274T (ATA→ACA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,647 C→A 36.0% V252L (GTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,713 G→A 24.5% L230L (CTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,319,721 T→G 5.2% pseudogene (584/1292 nt) EO946_RS17170 ← arabinogalactan endo‑beta‑1,4‑galactanase
RA 3,349,666 T→G 6.9% intergenic (+1/+26) EO946_RS17305 → / ← padC hypothetical protein/phenolic acid decarboxylase
RA 3,375,849 T→G 6.7% N263H (AAC→CAC)  trxB ← thioredoxin‑disulfide reductase
RA 3,446,962 C→A 12.2% M208I (ATG→ATT degU ← two‑component system response regulator DegU
RA 3,446,980 G→T 14.7% S202R (AGC→AGA degU ← two‑component system response regulator DegU
RA 3,447,038 T→G 11.5% N183T (AAC→ACC)  degU ← two‑component system response regulator DegU
RA 3,498,775 C→A 8.0% Q2K (CAA→AAA)  EO946_RS18005 → spore germination protein
RA 3,517,425 C→A 6.5% H93N (CAT→AAT)  EO946_RS18090 → SH3 domain‑containing protein
RA 3,539,638 G→T 8.3% P105P (CCC→CCA EO946_RS18215 ← T7SS effector LXG polymorphic toxin
RA 3,584,291 G→T 5.3% S324S (TCG→TCT EO946_RS18420 → N‑acetylneuraminate synthase family protein
RA 3,719,864 T→G 7.9% S79R (AGC→CGC)  EO946_RS19145 ← YwdI family protein
RA 3,721,872 T→G 5.0% E156D (GAA→GAC EO946_RS19155 ← uracil‑DNA glycosylase
RA 3,746,893 T→G 6.4% N59H (AAC→CAC)  EO946_RS19275 ← galactokinase
RA 3,746,916 G→T 6.4% T51K (ACA→AAA)  EO946_RS19275 ← galactokinase
RA 3,746,962 C→A 8.8% D36Y (GAT→TAT)  EO946_RS19275 ← galactokinase
RA 3,790,798 C→A 5.6% E191D (GAG→GAT EO946_RS19525 ← BglG family transcription antiterminator
RA 3,790,814 G→T 9.8% P186Q (CCA→CAA)  EO946_RS19525 ← BglG family transcription antiterminator
RA 3,846,645 C→A 6.1% D188Y (GAT→TAT)  dbpA ← ATP‑dependent RNA helicase DbpA
RA 3,851,220 G→T 9.3% T52K (ACG→AAG)  EO946_RS19820 ← hypothetical protein
RA 3,851,230 G→T 6.7% Q49K (CAG→AAG)  EO946_RS19820 ← hypothetical protein
RA 3,851,234 C→A 5.1% M47I (ATG→ATT EO946_RS19820 ← hypothetical protein
RA 3,851,237 G→T 8.1% I46I (ATC→ATA EO946_RS19820 ← hypothetical protein
RA 3,851,254 G→C 7.8% L41V (CTT→GTT)  EO946_RS19820 ← hypothetical protein
RA 3,851,262 C→A 18.2% W38L (TGG→TTG)  EO946_RS19820 ← hypothetical protein
RA 3,945,908 T→G 9.5% L388R (CTT→CGT)  EO946_RS20305 → Na+/H+ antiporter

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 91969NA (NA)15 (0.050) 11/250 NT NA intergenic (+131/‑39) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
?NZ_CP034943 91973 = NA (NA)intergenic (+135/‑35) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
* ? NZ_CP034943 607566 =207 (0.660)87 (0.280) 37/248 NT 22.9% coding (409/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607835 382 (1.240)coding (678/1125 nt) EO946_RS03185 collagen‑like protein