breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 218,269 +C coding (1042/1056 nt) llmg_0227 → putative membrane protein
RA 295,026 T→C S40P (TCG→CCG)  rimI2 → Ribosomal‑protein‑alanine acetyltransferase
RA 311,626 T→C C179R (TGC→CGC)  lmrC → multidrug resistance protein C
RA 447,854 G→A A98T (GCT→ACT)  llmg_0454 → putative beta‑glucosides‑specific IIBC component
RA 549,527 (T)6→7 intergenic (‑197/‑190) tnp905 ← / → tdk transposase for insertion sequence element IS905B/Tdk protein
RA 589,181 +C intergenic (+35/+231) llmg_0600 → / ← llmg_0601 glycosyl transferase/putative secreted protein
RA 594,325 T→A I263K (ATA→AAA)  recJ → single strand DNA‑specific exonuclease
RA 617,310 A→C S8S (TCA→TCC llmg_0628 → divalent cation transport‑related protein
RA 636,592 (A)7→6 coding (297/1164 nt) llmg_0642 ← putative membrane protein
RA 672,541 A→T L159L (CTA→CTT llmg_0681 → putative membrane protein
RA 732,731 +C coding (210/975 nt) malR → putative maltose operon transcriptional repressor, LacI family
RA 827,811 C→T intergenic (+153/‑32) uxuA → / → uxuT D‑mannonate hydrolase/Na‑galactoside symporter
RA 876,869 C→G H82D (CAT→GAT)  amtB → ammonium transporter AmtB
RA 894,627 +G coding (1044/1062 nt) llmg_0924 → conserved hypothetical protein
RA 912,618 T→A L46Q (CTA→CAA)  llmg_0944 → putative secreted protein
RA 912,718 T→A N79K (AAT→AAA llmg_0944 → putative secreted protein
RA 945,239 +C pseudogene (229/761 nt) tnp904 → pseudogene
RA 970,845 C→A A78D (GCT→GAT)  llmg_1005 → sugar kinase and transcriptional regulator
RA 977,528 Δ1 bp coding (1177/1197 nt) lplA → sugar ABC transporter substrate‑binding protein
RA 1,007,288 C→T F230F (TTC→TTT bglP → similar to PTS system, beta‑glucosides specific enzyme IIABC
RA 1,061,785 A→T I36N (ATT→AAT)  llmg_1101 ← putative secreted protein
RA 1,081,552 T→A V212D (GTT→GAT)  ldh → L‑lactate dehydrogenase
RA 1,093,299 +C coding (3979/4050 nt) llmg_1127 → cell wall surface anchor family protein
RA 1,093,562 +C coding (175/246 nt) llmg_1128 → hypothetical protein predicted by Glimmer/Critica
RA 1,201,775 (T)8→7 intergenic (+113/+2) llmg_1230 → / ← tnp1077 hypothetical protein predicted by Glimmer/Critica/pseudogene
RA 1,210,283 (A)5→6 pseudogene (515/1046 nt) llmg_pseudo_39 ← pseudogene
RA 1,223,555 Δ1 bp intergenic (+243/+302) llmg_1251 → / ← llmg_1252 hypothetical protein predicted by Glimmer/Critica/conserved hypothetical protein
RA 1,223,557 2 bp→AG intergenic (+245/+299) llmg_1251 → / ← llmg_1252 hypothetical protein predicted by Glimmer/Critica/conserved hypothetical protein
RA 1,277,684 T→A N231I (AAT→ATT)  llmg_1306 ← conserved hypothetical protein
RA 1,318,548 (A)7→6 coding (34/348 nt) llmg_1344 → hypothetical protein predicted by Glimmer/Critica
RA 1,492,365 T→A L262L (CTA→CTT kinE ← sensor kinase protein
RA 1,500,910 C→A D66E (GAC→GAA llmg_1528 → hypothetical protein predicted by Glimmer/Critica
RA 1,532,424 G→C P159R (CCC→CGC)  llmg_1560 ← stress induced DNA binding protein (putative)
RA 1,572,256 T→A intergenic (+54/+183) llmg_1598 → / ← deoD putative secreted protein/DeoD protein
RA 1,572,258 G→A intergenic (+56/+181) llmg_1598 → / ← deoD putative secreted protein/DeoD protein
RA 1,588,193 T→G Q297P (CAA→CCA)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,588,245 2 bp→AT coding (837‑838/1170 nt) ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,588,356 T→A N243Y (AAT→TAT)  ugd ← UDP‑glucose 6‑dehydrogenase
RA 1,610,761 T→C I430V (ATT→GTT)  llmg_1634 ← putative membrane protein
RA 1,614,130 +T coding (1149/1278 nt) mleP ← malate transporter
RA 1,681,037 T→C E332G (GAA→GGA)  pepV ← Dipeptidase
RA 1,731,090 T→C G335G (GGA→GGG llmg_1749 ← putative membrane protein
RA 1,783,142 G→A A603V (GCA→GTA)  llmg_1803 ← putative secreted protein
RA 1,817,902 C→A E463D (GAG→GAT xylS ← alpha‑xylosidase
RA 1,826,407 A→G Y12Y (TAT→TAC llmg_1845 ← metal‑dependent hydrolase
RA 1,826,465 (T)6→7 intergenic (‑23/+300) llmg_1845 ← / ← cspB metal‑dependent hydrolase/cold shock‑like protein cspB
RA 1,838,903 T→C T74A (ACT→GCT)  rmaB ← transcriptional regulator, MarR family
RA 1,853,472 C→A M85I (ATG→ATT glgP ← glycogen phosphorylase
RA 1,854,998 T→A V95V (GTA→GTT glgA ← GlgA protein
RA 1,891,450 C→A W87L (TGG→TTG)  llmg_1911 ← conserved hypothetical protein
RA 1,916,379 Δ1 bp pseudogene (355/598 nt) llmg_pseudo_59 ← pseudogene
RA 1,921,435 Δ1 bp coding (76/660 nt) llmg_1941 ← conserved hypothetical protein
RA 1,921,462 Δ1 bp coding (49/660 nt) llmg_1941 ← conserved hypothetical protein
RA 1,921,474 Δ1 bp coding (37/660 nt) llmg_1941 ← conserved hypothetical protein
RA 1,921,516 C→T intergenic (‑6/‑379) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,531 Δ1 bp intergenic (‑21/‑364) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,639 Δ1 bp intergenic (‑129/‑256) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,652 Δ1 bp intergenic (‑142/‑243) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,656 Δ1 bp intergenic (‑146/‑239) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,665 Δ1 bp intergenic (‑155/‑230) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,675 Δ1 bp intergenic (‑165/‑220) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,682 Δ1 bp intergenic (‑172/‑213) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,694 Δ1 bp intergenic (‑184/‑201) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,704 Δ1 bp intergenic (‑194/‑191) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,708 Δ1 bp intergenic (‑198/‑187) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,719 Δ1 bp intergenic (‑209/‑176) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,725 Δ1 bp intergenic (‑215/‑170) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,747 (T)5→4 intergenic (‑237/‑148) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,755 Δ1 bp intergenic (‑245/‑140) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,777 Δ1 bp intergenic (‑267/‑118) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,921,790 Δ1 bp intergenic (‑280/‑105) llmg_1941 ← / → glnP conserved hypothetical protein/glutamine ABC transporter permease and substrate binding protein
RA 1,933,214 2 bp→CG coding (224‑225/714 nt) atpB ← ATP synthase F0, A subunit
RA 1,935,405 (T)7→6 pseudogene (1748/2210 nt) comEC ← pseudogene
RA 1,942,432 T→C I80V (ATC→GTC)  llmg_1960 ← hypothetical protein predicted by Glimmer/Critica
RA 1,942,730 C→A intergenic (‑61/+328) llmg_1960 ← / ← tenA hypothetical protein predicted by Glimmer/Critica/transcriptional activator TenA
RA 2,044,751 G→A A37V (GCA→GTA)  ftsA ← cell division protein ftsA
RA 2,112,674 +T intergenic (‑79/+54) llmg_2151 ← / ← llmg_2152 Hypothetical protein ycaQ/Hypothetical protein ycaQ
RA 2,122,554 G→A S58L (TCG→TTG)  cfa ← cyclopropane‑fatty‑acyl‑phospholipid synthase
RA 2,122,558 C→T A57T (GCC→ACC)  cfa ← cyclopropane‑fatty‑acyl‑phospholipid synthase
RA 2,183,058 (A)5→4 coding (399/1161 nt) llmg_2218 ← predicted transcriptional regulator
RA 2,230,259 Δ1 bp coding (1758/1779 nt) llmg_2272 ← conserved hypothetical protein
RA 2,266,724 T→C I10T (ATT→ACT)  argR → arginine repressor
RA 2,492,912 G→A A203V (GCA→GTA)  gapB ← glyceraldehyde 3‑phosphate dehydrogenase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AM406671 495860 =67 (0.060)1195 (1.080) 275/294 0.1 94.7% intergenic (+60/‑49) llmg_0504/nifZ putative membrane protein/pyridoxal‑phosphate dependent aminotransferase
?AM406671 548108 = NA (NA)intergenic (+264/+47) llmg_0553/tnp905 conserved hypothetical protein/transposase for insertion sequence element IS905B
* ? AM406671 = 49586856 (0.050)1208 (1.090) 284/296 0.0 95.6% intergenic (+68/‑41) llmg_0504/nifZ putative membrane protein/pyridoxal‑phosphate dependent aminotransferase
?AM406671 = 549419 NA (NA)intergenic (‑89/‑298) tnp905/tdk transposase for insertion sequence element IS905B/Tdk protein
* ? AM406671 704944 =NA (NA)1012 (0.910) 209/296 2.6 NA coding (127/261 nt) llmg_0720 conserved hypothetical protein
?AM406671 = 796737 NA (NA)intergenic (+88/‑293) llmg_0823/ps333 putative transposase helper protein for IS712D/terminase small subunit
* ? AM406671 1583930 =NA (NA)1027 (0.930) 254/294 0.6 NA intergenic (‑98/‑80) glf2/tnp712 pseudogene/transposase for insertion sequence element IS712G
?AM406671 1632643 = NA (NA)coding (132/261 nt) llmg_1655 conserved hypothetical protein
* ? AM406671 2086138 =NA (NA)1313 (1.180) 288/296 0.0 95.3% intergenic (‑41/+48) ps442/tnp905 putative terminase large subunit/pseudogene
?AM406671 = 2090433 64 (0.060)coding (31/309 nt) ps435 hypothetical protein predicted by Glimmer/Critica
* ? AM406671 = 2087450NA (NA)1264 (1.140) 286/296 0.0 92.9% intergenic (‑89/+270) tnp905/ps440 pseudogene/phage terminase small subunit
?AM406671 2090426 = 97 (0.090)coding (38/309 nt) ps435 hypothetical protein predicted by Glimmer/Critica