breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 77,309 A→G intergenic (‑10/‑58) sgrR ← / → sgrS DNA‑binding transcriptional dual regulator SgrR/small regulatory RNA SgrS
RA 79,213 C→T G81S (GGC→AGC)  leuD ← 3‑isopropylmalate dehydratase subunit LeuD
RA 139,397 G→A A610V (GCA→GTA)  gcd ← quinoprotein glucose dehydrogenase
RA 188,435 C→T L72L (CTG→CTA glnD ← PII uridylyltransferase/uridylyl removing enzyme
RA 204,549 A→G G19G (GGA→GGG rnhB → RNase HII
RA 208,530 T→C R1135R (CGT→CGC dnaE → DNA polymerase III subunit alpha
RA 209,910 C→T P78S (CCG→TCG)  ldcC → lysine decarboxylase 2
RA 212,147 G→A A91T (GCG→ACG)  yaeR → VOC domain‑containing protein YaeR
RA 224,908 G→A noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA
RA 229,044 (T)8→7 intergenic (+40/‑123) aspU → / → dkgB tRNA‑Asp/methylglyoxal reductase DkgB
RA 247,805 C→T P57S (CCA→TCA)  rayT → REP‑associated tyrosine transposase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 259,478 T→C Y208C (TAT→TGT)  phoE ← outer membrane porin PhoE
RA 283,415 G→A G72D (GGC→GAC)  yagF → CP4‑6 prophage; D‑xylonate dehydratase
RA 297,695 A→G S33P (TCC→CCC)  yagP ← putative LysR family substrate binding domain‑containing protein YagP
RA 318,945 C→T A353T (GCT→ACT)  rclA ← putative pyridine nucleotide‑disulfide oxidoreductase RclA
RA 349,169 C→T A163V (GCG→GTG)  prpB → 2‑methylisocitrate lyase
RA 360,219 C→T A76A (GCC→GCT cynX → cyanate transporter
RA 399,101 A→G L78P (CTG→CCG)  yaiY ← inner membrane protein
RA 423,975 A→G G486G (GGA→GGG malZ → maltodextrin glucosidase
RA 426,273 T→C V46A (GTT→GCT)  tgt → tRNA‑guanine transglycosylase
RA 458,096 C→T P224L (CCG→CTG)  clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 484,656 T→C T322A (ACC→GCC)  acrA ← multidrug efflux pump membrane fusion lipoprotein AcrA
RA 525,148 A→G T630A (ACC→GCC)  rhsD → protein RhsD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 618,377 (A)7→6 coding (124/1134 nt) fepE → polysaccharide co‑polymerase family protein FepE
RA 622,460 T→C V54A (GTG→GCG)  entS → enterobactin exporter EntS
RA 625,033 A→G D50G (GAC→GGC)  entC → isochorismate synthase EntC
RA 811,946 T→C V142A (GTG→GCG)  bioC → malonyl‑acyl carrier protein methyltransferase
RA 829,356 G→A T206M (ACG→ATG)  ybhG ← HlyD_D23 family protein YbhG
RA 831,276 T→C R135R (CGT→CGC rhlE → ATP‑dependent RNA helicase RhlE
RA 877,527 G→A P170L (CCG→CTG)  rimO ← ribosomal protein S12 methylthiotransferase RimO
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 890,116 (G)5→6 coding (28/378 nt) ybjM → putative inner membrane protein
RA 899,761 C→G intergenic (+116/+83) rlmC → / ← artJ 23S rRNA m(5)U747 methyltransferase/L‑arginine ABC transporter periplasmic binding protein
RA 913,218 C→T A199A (GCG→GCA hcp ← protein S‑nitrosylase
RA 932,339 (A)8→6 intergenic (‑289/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 943,075 T→C V706A (GTT→GCT)  dmsA → dimethyl sulfoxide reductase subunit A
RA 964,379 G→A L20L (TTG→TTA ycaI → conserved inner membrane protein YcaI
RA 968,960 G→A G199S (GGT→AGT)  lpxK → tetraacyldisaccharide 4'‑kinase
RA 970,213 G→A A275A (GCG→GCA ycaQ → winged helix DNA‑binding domain‑containing protein YcaQ
RA 1,000,872 A→G T553A (ACG→GCG)  elfC → putative fimbrial usher protein ElfC
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,021,283 A→G Y49C (TAT→TGT)  sxy → transcriptional coactivator for CRP
RA 1,038,621 C→T I294I (ATC→ATT appC → cytochrome bd‑II ubiquinol oxidase subunit I
RA 1,064,681 G→A A216T (GCT→ACT)  yccE → uncharacterized protein YccE
RA 1,076,780 C→T T701T (ACG→ACA putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,077,020 C→T G621G (GGG→GGA putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,168,692 T→C E47E (GAA→GAG comR ← DNA‑binding transcriptional repressor ComR
RA 1,176,424 G→A G333D (GGC→GAC)  lolC → lipoprotein release complex ‑ inner membrane subunit
RA 1,190,871 G→A D372D (GAC→GAT purB ← adenylosuccinate lyase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,250,624 G→A A72V (GCC→GTC)  dhaK ← dihydroxyacetone kinase subunit K
RA 1,282,908 T→C G1015G (GGT→GGC narG → nitrate reductase A subunit alpha
RA 1,317,060 A→G S55P (TCC→CCC)  trpA ← tryptophan synthase subunit alpha
RA 1,322,274 G→A P225S (CCG→TCG)  trpE ← anthranilate synthase subunit TrpE
RA 1,367,267 T→C T216A (ACA→GCA)  pspF ← DNA‑binding transcriptional dual regulator PspF
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
RA 1,432,780 T→C F124L (TTC→CTC)  tfaR → Rac prophage; putative tail fiber assembly protein TfaR
RA 1,444,488 A→G M480V (ATG→GTG)  ydbH → PF11739 family protein YdbH
RA 1,501,651 A→G K30K (AAA→AAG tehB → tellurite methyltransferase
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,543,638 T→C A141A (GCA→GCG narU ← nitrate/nitrite transporter NarU
RA 1,545,403 T→C P104P (CCA→CCG yddK ← leucine‑rich repeat domain‑containing protein YddK
RA 1,558,997 G→A D7D (GAC→GAT ddpD ← putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpD
RA 1,564,014 C→T G574S (GGC→AGC)  dosP ← oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
RA 1,575,914 T→C Y569C (TAC→TGC)  yddB ← putative TonB‑dependent receptor
RA 1,576,542 T→C T360A (ACA→GCA)  yddB ← putative TonB‑dependent receptor
RA 1,586,334 A→G G51G (GGT→GGC ydeP ← putative oxidoreductase YdeP
RA 1,594,132 (C)8→9 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,607,789 A→G E148E (GAA→GAG tam → trans‑aconitate 2‑methyltransferase
RA 1,614,700 T→C T2A (ACC→GCC)  sad ← NAD(+)‑dependent succinate semialdehyde dehydrogenase
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,635,293 G→A T185T (ACC→ACT ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,296 T→G G184G (GGA→GGC ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,322 C→T E176K (GAA→AAA)  ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,335 C→T T171T (ACG→ACA ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,362 G→A H162H (CAC→CAT ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,392 T→C L152L (TTA→TTG ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,413 G→A P145P (CCC→CCT ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,416 C→T K144K (AAG→AAA ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,673,662 A→G intergenic (‑161/‑251) mdtJ ← / → tqsA multidrug/spermidine efflux pump membrane subunit MdtJ/autoinducer 2 exporter
RA 1,683,057 (C)6→7 coding (176/1302 nt) rstB → sensory histidine kinase RstB
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,692,675 T→C V59V (GTA→GTG uidC ← outer membrane porin family protein
RA 1,698,408 G→A A258V (GCA→GTA)  malI ← DNA‑binding transcriptional repressor MalI
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,731,858 A→G E924E (GAA→GAG lhr → putative ATP‑dependent helicase Lhr
RA 1,745,702 G→A G142G (GGC→GGT ydhQ ← adhesin‑like autotransporter YdhQ
RA 1,756,910 (A)6→5 coding (1213/1413 nt) pykF → pyruvate kinase I
RA 1,759,081 Δ1 bp coding (210/417 nt) sufE ← sulfur acceptor for SufS cysteine desulfurase
RA 1,764,602 A→G intergenic (‑216/+111) sufA ← / ← rydB iron‑sulfur cluster insertion protein SufA/small regulatory RNA RydB
RA 1,774,849 T→C L55P (CTC→CCC)  aroD → 3‑dehydroquinate dehydratase
RA 1,792,581 G→A R51C (CGT→TGT)  nlpC ← NlpC/P60 family lipoprotein NlpC
RA 1,793,378 G→A Q61* (CAA→TAA)  btuD ← vitamin B12 ABC transporter ATP binding subunit
RA 1,808,608 G→A Q271Q (CAG→CAA yniA → putative kinase YniA
RA 1,816,136 C→T intergenic (+8/+250) katE → / ← chbG catalase II/chitin disaccharide deacetylase
RA 1,829,911 G→A G285G (GGC→GGT astA ← arginine N‑succinyltransferase
RA 1,831,404 (C)5→6 coding (579/1221 nt) astC ← succinylornithine transaminase
RA 1,840,945 T→C G153G (GGA→GGG ynjF ← putative phosphatidyl transferase, inner membrane protein
RA 1,863,500 A→G T244A (ACC→GCC)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,874,642 G→A W97* (TGG→TGA yeaL → conserved inner membrane protein YeaL
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,576 C→A A52A (GCG→GCT yoaH ← UFP0181 family protein YoaH
RA 1,914,686 A→G V44A (GTA→GCA)  prc ← tail‑specific protease
RA 1,915,022 T→C A171A (GCA→GCG proQ ← RNA chaperone ProQ
RA 1,924,272 A→G V107A (GTC→GCC)  yebZ ← putative inner membrane protein
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,982,897 A→G V215A (GTA→GCA)  araH ← arabinose ABC transporter membrane subunit
RA 2,034,114 G→A pseudogene (64/1193 nt) yedS → putative outer membrane protein YedS, N‑terminal fragment
RA 2,037,021 T→C T378A (ACC→GCC)  hprS ← sensory histidine kinase HprS
RA 2,065,747 (C)6→5 coding (18/546 nt) cobU ← cobinamide‑P guanylyltransferase/cobinamide kinase
RA 2,074,704 C→T intergenic (+46/‑75) flu → / → yeeR CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter/CP4‑44 prophage; inner membrane protein YeeR
JC 2,090,116 (C)5→8 intergenic (+70/‑76) hisL → / → hisG his operon leader peptide/ATP phosphoribosyltransferase
RA 2,097,780 T→C G174G (GGA→GGG wzzB ← regulator of length of O‑antigen component of lipopolysaccharide chains
RA 2,136,972 T→C D91G (GAC→GGC)  wza ← outer membrane polysaccharide export protein Wza
RA 2,158,475 T→C R30R (CGT→CGC mdtC → multidrug efflux pump RND permease subunit MdtC
RA 2,165,693 C→T P9L (CCG→CTG)  yegQ → putative peptidase YegQ
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,197,957 C→T intergenic (+659/‑2322) yehF → / → yehI putative protein YehF/DUF4132 domain‑containing protein YehI
RA 2,225,640 (C)5→6 intergenic (‑9/‑161) yohC ← / → yohD putative inner membrane protein/DedA family protein YohD
RA 2,267,921 (A)5→4 coding (93/987 nt) yeiR → zinc‑binding GTPase YeiR
RA 2,296,397 C→A A210S (GCG→TCG)  ccmB ← heme trafficking system membrane protein CcmB
RA 2,302,512 C→T C81Y (TGC→TAC)  napA ← periplasmic nitrate reductase subunit NapA
RA 2,313,304 G→A intergenic (+128/‑184) micF → / → rcsD small regulatory RNA MicF/RcsD phosphotransferase
RA 2,317,764 T→C S705G (AGT→GGT)  rcsC ← sensory histidine kinase RcsC
RA 2,377,318 G→A A92V (GCG→GTG)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,379,609 A→G V345V (GTT→GTC menF ← isochorismate synthase MenF
RA 2,441,361 G→A A82V (GCA→GTA)  fabB ← beta‑ketoacyl‑[acyl carrier protein] synthase I
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,464,775 A→G L78P (CTT→CCT)  mlaA ← intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,577,589 A→G C263C (TGT→TGC maeB ← malate dehydrogenase
RA 2,586,590 T→C V287A (GTC→GCC)  narQ → sensory histidine kinase NarQ
RA 2,591,557 T→C L104P (CTG→CCG)  yffB → putative reductase YffB
RA 2,612,556 A→G P219P (CCA→CCG hyfR → DNA‑binding transcriptional activator HyfR
RA 2,646,583 (G)6→7 coding (743/2313 nt) pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,649,354 G→A N978N (AAC→AAT yfhM ← alpha2‑macroglobulin
RA 2,679,678 G→A A1023V (GCC→GTC)  yphG ← DUF5107 domain‑containing protein YphG
RA 2,680,184 C→T L854L (CTG→CTA yphG ← DUF5107 domain‑containing protein YphG
RA 2,681,870 T→C G292G (GGA→GGG yphG ← DUF5107 domain‑containing protein YphG
RA 2,720,083 T→C V44A (GTA→GCA)  patZ → peptidyl‑lysine acetyltransferase
RA 2,734,543 (C)8→10 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,759,743 (T)6→7 coding (759/1410 nt) yfjI → CP4‑57 prophage; protein YfjI
RA 2,782,600 T→C T43A (ACT→GCT)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,801,601 T→C V85A (GTC→GCC)  nrdE → ribonucleoside‑diphosphate reductase 2 subunit alpha
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,812,983 C→T S123L (TCG→TTG)  emrB → multidrug efflux pump membrane subunit EmrB
RA 2,821,292 A→G G240G (GGT→GGC alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,821,424 G→C G196G (GGC→GGG alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,821,923 A→G V30A (GTA→GCA)  alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,841,831 T→C V281A (GTA→GCA)  ascB → 6‑phospho‑beta‑glucosidase AscB
RA 2,842,550 T→C intergenic (+136/+23) ascB → / ← hycI 6‑phospho‑beta‑glucosidase AscB/hydrogenase 3 maturation protease
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,900,306 (A)9→8 intergenic (‑33/‑286) ygcW ← / → yqcE putative deoxygluconate dehydrogenase/putative transport protein YqcE
RA 2,909,883 G→A intergenic (‑217/+11) pyrG ← / ← mazG CTP synthetase/nucleoside triphosphate pyrophosphohydrolase
RA 2,910,815 T→C T93A (ACA→GCA)  mazF ← endoribonuclease toxin MazF
RA 2,947,755 T→C intergenic (+72/+2) metV → / ← amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,964,718 G→A C146C (TGC→TGT thyA ← thymidylate synthase
RA 2,965,681 A→G R119R (CGT→CGC lgt ← phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase
RA 2,978,815 A→G W29R (TGG→CGG)  lysA ← diaminopimelate decarboxylase
RA 3,010,338 G→A R104H (CGC→CAC)  hyuA → phenylhydantoinase
RA 3,059,012 A→T I105N (ATC→AAC)  rpiA ← ribose‑5‑phosphate isomerase A
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,093,479 T→C P181P (CCT→CCC yqgE → DUF179 domain‑containing protein YqgE
RA 3,101,039 (C)7→8 coding (585/720 nt) yggN ← putative EcfF
RA 3,126,083 T→C Y147C (TAC→TGC)  glcE ← glycolate dehydrogenase, putative FAD‑binding subunit
RA 3,146,246 T→C T6A (ACC→GCC)  hybO ← hydrogenase 2 small subunit
RA 3,163,303 T→C D60G (GAC→GGC)  plsC ← 1‑acylglycerol‑3‑phosphate O‑acyltransferase
RA 3,231,336 A→T intergenic (+97/‑329) ygjK → / → fadH glycoside hydrolase/2,4‑dienoyl‑CoA reductase
RA 3,231,367 A→G intergenic (+128/‑298) ygjK → / → fadH glycoside hydrolase/2,4‑dienoyl‑CoA reductase
RA 3,231,411 G→A intergenic (+172/‑254) ygjK → / → fadH glycoside hydrolase/2,4‑dienoyl‑CoA reductase
RA 3,231,417 A→G intergenic (+178/‑248) ygjK → / → fadH glycoside hydrolase/2,4‑dienoyl‑CoA reductase
RA 3,231,425 C→T intergenic (+186/‑240) ygjK → / → fadH glycoside hydrolase/2,4‑dienoyl‑CoA reductase
RA 3,250,696 T→C G45G (GGT→GGC yqjF → DoxX family protein
RA 3,251,988 G→A G322E (GGA→GAA)  yqjG → glutathionyl‑hydroquinone reductase YqjG
RA 3,253,810 (C)5→4 coding (405/897 nt) yhaJ ← DNA‑binding transcriptional activator YhaJ
RA 3,294,876 T→C S493P (TCT→CCT)  lpoA → outer membrane lipoprotein ‑ activator of MrcA activity
JC 3,304,813 (C)7→10 coding (1005/1245 nt) mtr ← tryptophan:H(+) symporter Mtr
RA 3,337,788 G→A A35V (GCG→GTG)  mlaC ← intermembrane phospholipid transport system ‑ periplasmic binding protein
RA 3,341,464 (A)5→6 coding (199/987 nt) kdsD → D‑arabinose 5‑phosphate isomerase KdsD
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,393,438 G→A R941C (CGT→TGT)  yhdP ← AsmA2 domain‑containing protein
RA 3,465,949 G→A G136E (GGG→GAG)  gspO → Type II secretion system prepilin peptidase
RA 3,510,617 G→A G120G (GGC→GGT yhfX ← putative PLP‑binding protein YhfX
RA 3,538,681 T→C F433L (TTC→CTC)  yhgF → putative RNA‑binding protein YhgF
RA 3,548,667 G→A G468G (GGC→GGT malQ ← 4‑alpha‑glucanotransferase
RA 3,550,853 G→A R541C (CGC→TGC)  malP ← maltodextrin phosphorylase
RA 3,553,728 T→C V215A (GTT→GCT)  malT → DNA‑binding transcriptional activator MalT
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,584,131 T→A L152Q (CTG→CAG)  insB‑6 → IS1 protein InsB
RA 3,588,404 A→G G259G (GGT→GGC ugpC ← sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
RA 3,606,249 T→C L167L (TTG→CTG)  yhhN → conserved inner membrane enzyme YhhN
RA 3,617,710 (C)5→6 coding (696/765 nt) nikD → Ni(2(+)) ABC transporter ATP binding subunit NikD
RA 3,619,221 T→C G10G (GGT→GGC rhsB → rhs element protein RhsB
RA 3,619,251 T→C V20V (GTT→GTC rhsB → rhs element protein RhsB
RA 3,619,272 T→C R27R (CGT→CGC rhsB → rhs element protein RhsB
RA 3,619,275 T→C I28I (ATT→ATC rhsB → rhs element protein RhsB
RA 3,638,467 C→T Q276* (CAG→TAG)  pitA → metal phosphate:H(+) symporter PitA
RA 3,643,621 T→C H521R (CAC→CGC)  prlC ← oligopeptidase A
RA 3,645,505 A→G T41A (ACC→GCC)  rlmJ → 23S rRNA m(6)A2030 methyltransferase
RA 3,679,422 T→C S2P (TCC→CCC)  kdgK → 2‑dehydro‑3‑deoxygluconokinase
RA 3,711,727 G→A A94V (GCG→GTG)  yhjX ← putative pyruvate transporter
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,817,019 T→C V115A (GTG→GCG)  yicC → UPF0701 family protein YicC
RA 3,820,757 T→C K138K (AAA→AAG ligB ← DNA ligase B
RA 3,856,624 (A)6→7 coding (210/450 nt) yidI → putative inner membrane protein
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,873,084 T→C S177G (AGC→GGC)  dgoA ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA 3,877,329 G→C A46G (GCC→GGC)  yidB ← conserved protein YidB
RA 3,880,097 G→A S8F (TCC→TTC)  gyrB ← DNA gyrase subunit B
RA 3,881,817 T→C T169A (ACC→GCC)  dnaN ← beta sliding clamp
RA 3,886,716 (A)6→5 coding (1641/1647 nt) yidC → membrane protein insertase YidC
RA 3,992,297 C→T A382V (GCG→GTG)  cyaA → adenylate cyclase
RA 4,026,219 A→G N411S (AAC→AGC)  ubiD → 3‑octaprenyl‑4‑hydroxybenzoate decarboxylase
RA 4,035,253 A→G intergenic (+100/‑278) hemG → / → rrsA protoporphyrinogen oxidase/16S ribosomal RNA
RA 4,065,906 A→G V430A (GTA→GCA)  yihP ← putative 2,3‑dihydroxypropane‑1‑sulfonate export protein
RA 4,079,837 C→T D29D (GAC→GAT yiiF → protein YiiF
RA 4,085,268 A→G G185G (GGT→GGC fdoG ← formate dehydrogenase O subunit alpha
RA 4,093,535 G→A G246G (GGC→GGT rhaD ← rhamnulose‑1‑phosphate aldolase
RA 4,094,489 G→A intergenic (‑217/+234) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,168,472 G→A intergenic (+25/‑169) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA
RA 4,168,475 2 bp→TG intergenic (+28/‑165) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA
RA 4,194,668 A→G S478P (TCG→CCG)  thiC ← phosphomethylpyrimidine synthase
RA 4,222,704 A→G intergenic (‑217/+100) arpA ← / ← iclR regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR
RA 4,245,645 A→G S259P (TCC→CCC)  malE ← maltose ABC transporter periplasmic binding protein
RA 4,267,511 A→G N133S (AAC→AGC)  tyrB → tyrosine aminotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,309,020 C→T L150L (CTG→CTA alsC ← D‑allose ABC transporter membrane subunit
RA 4,319,195 C→T E138K (GAA→AAA)  phnJ ← carbon‑phosphorus lyase core complex subunit PhnJ
RA 4,341,808 C→T intergenic (‑180/‑103) melR ← / → melA DNA‑binding transcriptional dual regulator MelR/alpha‑galactosidase
RA 4,386,559 C→T D938N (GAT→AAT)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,486,192 G→A intergenic (‑241/‑26) pepA ← / → lptF aminopeptidase A/I/lipopolysaccharide transport system protein LptF
RA 4,494,288 C→A C40F (TGC→TTC)  idnD ← L‑idonate 5‑dehydrogenase
RA 4,496,087 G→A C41C (TGC→TGT ahr ← aldehyde reductase, NADPH‑dependent
RA 4,536,190 T→C intergenic (+159/+424) yjhR → / ← nanS KpLE2 phage‑like element; PLD‑like domain‑containing protein YjhR/N‑acetyl‑9‑O‑acetylneuraminate esterase
RA 4,606,095 Δ1 bp noncoding (71/87 nt) leuV ← tRNA‑Leu
JC 4,606,215 (G)8→11 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,631,477 T→C F249L (TTT→CTT)  slt → soluble lytic murein transglycosylase
RA 4,633,457 C→T G97S (GGC→AGC)  yjjX ← ITPase/XTPase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196260 1211438 15179 139 [137] [133] 146 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423755–3424545 3424545 1–791 143 [128] [136] 147 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)536 (1.230) 143/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =933 (2.070)937 (2.100) 143/274 0.1 66.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 15 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)488 (1.080) 126/276 0.3 94.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 27 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA