breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 310 | (T)9→8 | intergenic (+55/‑27) | thrL → / → thrA | thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1 |
RA | 40,906 | A→G | R342R (CGT→CGC) | caiT ← | L‑carnitine:gamma‑butyrobetaine antiporter |
RA | 48,192 | A→G | T142A (ACG→GCG) | kefC → | K(+) : H(+) antiporter KefC |
RA | 63,248 | C→T | G6D (GGT→GAT) | rapA ← | RNA polymerase‑binding ATPase and RNAP recycling factor |
RA | 69,104 | A→G | G315G (GGT→GGC) | araB ← | ribulokinase |
RA | 194,885 | T→C | intergenic (+168/‑18) | dxr → / → ispU | 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase/undecaprenyl diphosphate synthase |
RA | 199,200 | A→G | S425G (AGC→GGC) | bamA → | outer membrane protein assembly factor BamA |
RA | 218,835 | (A)8→9 | intergenic (‑60/+52) | proS ← / ← trmO | proline‑‑tRNA ligase/tRNA m(6)t(6)A37 methyltransferase |
RA | 243,760 | A→G | N73S (AAC→AGC) | lpcA → | D‑sedoheptulose 7‑phosphate isomerase |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
RA | 301,619 | A→G | V90A (GTA→GCA) | paoB ← | aldehyde dehydrogenase, FAD‑binding subunit |
RA | 307,306 | T→C | D676G (GAC→GGC) | ecpC ← | putative fimbrial usher protein EcpC |
RA | 308,054 | (C)7→8 | coding (1279/2526 nt) | ecpC ← | putative fimbrial usher protein EcpC |
RA | 321,564 | (G)7→8 | intergenic (+483/‑44) | rclR → / → ykgE | DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE |
RA | 365,310 | A→G | G332G (GGT→GGC) | lacZ ← | beta‑galactosidase |
RA | 380,022 | (G)10→9 | intergenic (‑141/+47) | frmR ← / ← yaiO | DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO |
RA | 412,372 | A→G | I37T (ATT→ACT) | araJ ← | putative transport protein AraJ |
RA | 453,447 | A→G | G33G (GGT→GGC) | ampG ← | muropeptide:H(+) symporter |
RA | 483,232 | T→C | N391S (AAC→AGC) | acrB ← | multidrug efflux pump RND permease AcrB |
RA | 502,496 | A→G | V248A (GTC→GCC) | ybaL ← | putative transporter YbaL |
RA | 527,577 | T→C | intergenic (+36/‑4) | rhsD → / → ybbC | protein RhsD/PF15631 family protein YbbC |
RA | 527,730 | A→G | V50V (GTA→GTG) | ybbC → | PF15631 family protein YbbC |
RA | 552,997 | A→G | V35A (GTC→GCC) | purE ← | N(5)‑carboxyaminoimidazole ribonucleotide mutase |
RA | 699,536 | T→C | intergenic (‑359/+38) | asnB ← / ← umpH | asparagine synthetase B/UMP phosphatase |
RA | 768,190 | C→T | D71D (GAC→GAT) | mngB → | alpha‑mannosidase |
RA | 777,145 | (A)5→6 | coding (804/1266 nt) | tolA → | Tol‑Pal system protein TolA |
RA | 778,662 | A→G | Q308R (CAG→CGG) | tolB → | Tol‑Pal system periplasmic protein TolB |
RA | 878,840 | A→G | K33K (AAA→AAG) | yliI → | aldose sugar dehydrogenase YliI |
RA | 889,923 | A→G | intergenic (‑104/‑166) | ybjL ← / → ybjM | putative transport protein YbjL/putative inner membrane protein |
RA | 922,071 | A→G | E575E (GAA→GAG) | macB → | ABC‑type tripartite efflux pump ATP binding/membrane subunit |
RA | 932,340 | (A)8→7 | intergenic (‑290/‑255) | trxB ← / → lrp | thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp |
RA | 945,259 | A→G | V100A (GTC→GCC) | ycaC ← | putative hydrolase |
RA | 962,213 | C→T | D73D (GAC→GAT) | rpsA → | 30S ribosomal subunit protein S1 |
RA | 980,123 | G→A | S1266S (TCG→TCA) | mukB → | chromosome partitioning protein MukB |
RA | 1,010,898 | C→T | A312V (GCC→GTC) | uup → | ATP‑binding protein with possible role in replication |
RA | 1,011,593 | (A)7→8 | coding (1630/1908 nt) | uup → | ATP‑binding protein with possible role in replication |
RA | 1,059,436 | (G)6→7 | coding (181/2547 nt) | torA → | trimethylamine N‑oxide reductase 1 |
RA | 1,100,097 | C→G | A153G (GCC→GGC) | ycdY → | chaperone protein YcdY |
RA | 1,106,134 | A→G | L105L (CTA→CTG) | ymdB → | 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity |
RA | 1,143,140 | T→C | T410A (ACT→GCT) | rne ← | ribonuclease E |
RA | 1,220,971 | G→A | pseudogene (1371/2648 nt) | ycgH → | putative transporter component, N‑terminal fragment |
RA | 1,275,482 | C→T | L116L (CTG→CTA) | narL ← | DNA‑binding transcriptional dual regulator NarL |
RA | 1,283,686 | (A)5→4 | coding (83/1539 nt) | narH → | nitrate reductase A subunit beta |
RA | 1,286,570 | (T)7→6 | intergenic (+44/‑139) | narI → / → ychS | nitrate reductase A subunit gamma/putative uncharacterized protein YchS |
RA | 1,291,035 | T→C | V265A (GTT→GCT) | rssB → | regulator of RpoS |
RA | 1,296,844 | A→G | G426G (GGT→GGC) | adhE ← | aldehyde‑alcohol dehydrogenase |
RA | 1,323,030 | T→C | intergenic (‑84/+8) | trpE ← / ← trpL | anthranilate synthase subunit TrpE/trp operon leader peptide |
JC | 1,351,196 | (G)7→10 | intergenic (‑157/+211) | fabI ← / ← ycjD | enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD |
RA | 1,398,741 | T→C | intergenic (‑119/+33) | uspE ← / ← fnr | universal stress protein with a role cellular motility/DNA‑binding transcriptional dual regulator FNR |
RA | 1,491,129 | A→G | K76K (AAA→AAG) | cybB → | cytochrome b561 |
RA | 1,541,351 | C→T | E414K (GAA→AAA) | narZ ← | nitrate reductase Z subunit alpha |
RA | 1,543,407 | T→C | V218V (GTA→GTG) | narU ← | nitrate/nitrite transporter NarU |
RA | 1,567,363 | A→G | intergenic (‑144/+141) | yddY ← / ← yddW | protein YddY/putative lipoprotein YddW |
JC | 1,594,132 | (C)8→11 | pseudogene (3955/5422 nt) | yneO ← | AIDA‑I family autotransporter YneO |
RA | 1,634,642 | C→T | G82S (GGC→AGC) | tfaQ ← | Qin prophage; putative tail fiber assembly protein TfaQ |
RA | 1,643,501 | T→C | T268T (ACA→ACG) | ydfU ← | Qin prophage; protein YdfU |
RA | 1,660,581 | T→C | L9P (CTC→CCC) | ynfF → | putative selenate reductase YnfF |
RA | 1,681,163 | A→C | D63A (GAC→GCC) | folM → | dihydromonapterin reductase |
RA | 1,685,542 | (C)6→5 | coding (1047/1404 nt) | fumC ← | fumarase C |
RA | 1,746,014 | G→A | G38G (GGC→GGT) | ydhQ ← | adhesin‑like autotransporter YdhQ |
RA | 1,849,310 | Δ1 bp | coding (474/1857 nt) | sppA → | protease IV, a signal peptide peptidase |
RA | 1,873,384 | A→G | K448K (AAA→AAG) | dgcJ → | putative diguanylate cyclase DgcJ |
RA | 1,875,056 | (C)6→7 | coding (521/822 nt) | nimR ← | DNA‑binding transcriptional repressor NimR |
JC JC | 1,879,829 | Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp | coding (115‑120/360 nt) | yeaR ← | DUF1971 domain‑containing protein YeaR |
RA | 1,885,910 | A→G | K22K (AAA→AAG) | yeaX → | carnitine monooxygenase subunit YeaX |
RA | 1,921,492 | T→C | W424R (TGG→CGG) | rsmF → | 16S rRNA m(5)C1407 methyltransferase |
MC JC | 1,978,503 | Δ776 bp | insB‑5–insA‑5 | insB‑5, insA‑5 | |
RA | 2,008,322 | G→C | A16P (GCC→CCC) | yedE → | putative selenium transporter YedE |
RA | 2,123,008 | G→A | intergenic (‑29/+76) | cpsG ← / ← cpsB | phosphomannomutase/mannose‑1‑phosphate guanylyltransferase |
RA | 2,173,363 | Δ2 bp | pseudogene (915‑916/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
RA | 2,188,193 | T→C | G74G (GGA→GGG) | yehA ← | putative fimbrial adhesin YehA |
RA | 2,274,432 | T→C | V85A (GTG→GCG) | yejB → | putative oligopeptide ABC transporter membrane subunit YejB |
RA | 2,339,970 | T→C | V135A (GTC→GCC) | ubiG → | bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase |
RA | 2,446,579 | T→C | T299A (ACC→GCC) | aroC ← | chorismate synthase |
RA | 2,527,823 | T→C | V293A (GTG→GCG) | yfeH → | putative solute:Na(+) symporter |
RA | 2,592,616 | T→C | V337A (GTG→GCG) | dapE → | succinyl‑diaminopimelate desuccinylase |
RA | 2,602,017 | (C)7→8 | coding (200/2019 nt) | hyfB → | hydrogenase 4 component B |
RA | 2,603,074 | T→C | R419R (CGT→CGC) | hyfB → | hydrogenase 4 component B |
RA | 2,630,822 | T→C | V166A (GTG→GCG) | yfgI → | nalidixic acid resistance protein YfgI |
RA | 2,644,674 | A→G | V64A (GTT→GCT) | ndk ← | nucleoside diphosphate kinase |
RA | 2,687,452 | T→C | intergenic (‑44/+17) | glnB ← / ← glrR | nitrogen regulatory protein PII‑1/DNA‑binding transcriptional activator GlrR |
RA | 2,731,729 | A→G | R815R (CGT→CGC) | clpB ← | ClpB chaperone |
RA | 2,734,543 | (C)8→9 | coding (492/732 nt) | pgeF ← | polyphenol oxidase YfiH |
RA | 2,740,439 | Δ1 bp | coding (712/1071 nt) | aroF ← | 3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive |
RA | 2,799,453 | T→C | V97A (GTG→GCG) | alaE → | L‑alanine exporter |
RA | 2,811,134 | A→G | H122R (CAC→CGC) | mprA → | DNA‑binding transcriptional repressor MprA |
RA | 2,824,478 | (C)6→7 | intergenic (‑132/+13) | pncC ← / ← mltB | NMN aminohydrolase/membrane‑bound lytic murein transglycosylase B |
RA | 2,839,731 | A→G | T70A (ACC→GCC) | ascF → | beta‑glucoside specific PTS enzyme IIBC component |
MC JC | 2,857,096 | Δ2,538 bp | coding (4‑2541/2562 nt) | mutS → | DNA mismatch repair protein MutS |
RA | 2,883,287 | A→G | C284C (TGT→TGC) | casA ← | type I‑E CRISPR system Cascade subunit CasA |
RA | 2,897,937 | Δ1 bp | intergenic (‑67/+27) | ygcS ← / ← ygcU | putative transporter YgcS/putative FAD‑containing dehydrogenase |
RA | 2,906,780 | C→T | A388T (GCT→ACT) | eno ← | enolase |
RA | 2,910,916 | G→A | P59L (CCG→CTG) | mazF ← | endoribonuclease toxin MazF |
RA | 2,920,095 | T→C | T218A (ACT→GCT) | gudX ← | glucarate dehydratase‑related protein |
RA | 2,942,553 | T→C | L5L (TTA→TTG) | gcvA ← | DNA‑binding transcriptional dual regulator GcvA |
RA | 2,949,993 | (T)5→6 | coding (752/1332 nt) | argA → | N‑acetylglutamate synthase |
RA | 2,968,911 | T→C | M23V (ATG→GTG) | rppH ← | RNA pyrophosphohydrolase |
RA | 2,978,049 | A→G | V284A (GTG→GCG) | lysA ← | diaminopimelate decarboxylase |
RA | 2,978,378 | T→C | A174A (GCA→GCG) | lysA ← | diaminopimelate decarboxylase |
RA | 2,994,310 | G→A | pseudogene (372/503 nt) | pbl → | transglycosylase domain‑containing protein Pbl |
RA | 2,999,680 | (G)7→8 | coding (212/756 nt) | ygeR ← | LysM domain‑containing putative peptidase lipoprotein YgeR |
RA | 3,039,599 | T→C | T49A (ACG→GCG) | xerD ← | site‑specific recombinase |
RA | 3,068,157 | T→C | intergenic (‑77/+16) | yggE ← / ← argO | uncharacterized protein YggE/L‑arginine exporter |
RA | 3,151,539 | A→G | V149A (GTC→GCC) | exbB ← | Ton complex subunit ExbB |
RA | 3,192,793 | A→G | T196A (ACG→GCG) | yqiJ → | putative inner membrane protein |
RA | 3,230,773 | T→C | V629A (GTA→GCA) | ygjK → | glycoside hydrolase |
RA | 3,252,614 | +A | coding (311/366 nt) | yhaH → | putative inner membrane protein |
RA | 3,274,206 | T→C | A234A (GCA→GCG) | garP ← | galactarate/glucarate/glycerate transporter GarP |
RA | 3,292,985 | C→T | V117V (GTG→GTA) | rsmI ← | 16S rRNA 2'‑O‑ribose C1402 methyltransferase |
RA | 3,305,589 | T→C | T77A (ACC→GCC) | mtr ← | tryptophan:H(+) symporter Mtr |
RA | 3,354,487 | C→T | intergenic (‑437/‑238) | yhcC ← / → gltB | radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB |
RA | 3,354,895 | G→A | L57L (TTG→TTA) | gltB → | glutamate synthase subunit GltB |
RA | 3,388,726 | A→G | V134A (GTT→GCT) | aaeA ← | aromatic carboxylic acid efflux pump membrane fusion protein |
RA | 3,456,195 | A→G | L206L (CTA→CTG) | gspC → | Type II secretion system protein GspC |
RA | 3,499,074 | A→G | Q416R (CAG→CGG) | cysG → | siroheme synthase |
RA | 3,521,612 | T→C | T121A (ACG→GCG) | hofN ← | DNA utilization protein HofN |
RA | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
RA | 3,640,471 | T→C | S121P (TCT→CCT) | uspA → | universal stress global stress response regulator |
RA | 3,658,602 | A→G | K79K (AAA→AAG) | gadE → | DNA‑binding transcriptional activator GadE |
RA | 3,664,363 | (G)6→7 | coding (256/729 nt) | gadW ← | DNA‑binding transcriptional dual regulator GadW |
RA | 3,728,118 | (C)8→7 | coding (1254/1455 nt) | xylB ← | xylulokinase |
RA | 3,779,008 | T→C | V537A (GTG→GCG) | lldP → | lactate/glycolate:H(+) symporter LldP |
RA | 3,782,359 | T→C | E68E (GAA→GAG) | cysE ← | serine acetyltransferase |
RA | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
RA | 3,834,428 | (G)6→7 | coding (110/2319 nt) | yicI ← | alpha‑D‑xyloside xylohydrolase |
RA | 3,860,630 | (C)5→4 | coding (348/894 nt) | yidL → | putative DNA‑binding transcriptional regulator YidL |
RA | 3,863,987 | (A)7→8 | coding (89/717 nt) | yidP → | putative DNA‑binding transcriptional regulator YidP |
RA | 3,870,267 | T→C | R297R (CGT→CGC) | cbrA → | colicin M resistance protein |
RA | 3,874,882 | C→T | R93R (CGG→CGA) | dgoR ← | putative DNA‑binding transcriptional regulator DgoR |
RA | 3,894,975 | T→C | G108G (GGT→GGC) | yieF → | chromate reductase |
RA | 3,961,491 | A→G | K272R (AAG→AGG) | rep → | ATP‑dependent DNA helicase Rep |
RA | 3,969,767 | T→C | V246A (GTA→GCA) | wzzE → | enterobacterial common antigen polysaccharide co‑polymerase |
RA | 3,996,724 | T→C | G146G (GGT→GGC) | xerC → | site‑specific tyrosine recombinase |
RA | 4,027,317 | A→G | intergenic (+89/+292) | fre → / ← fadA | flavin reductase/3‑ketoacyl‑CoA thiolase |
RA | 4,050,693 | T→C | T25A (ACC→GCC) | yihA ← | GTP‑binding protein YihA |
RA | 4,062,890 | T→C | V215A (GTG→GCG) | yihN → | putative transporter YihN |
RA | 4,086,523 | A→G | T170A (ACT→GCT) | fdhD → | sulfurtransferase for molybdenum cofactor sulfuration |
RA | 4,103,043 | A→G | E74E (GAA→GAG) | yiiM → | 2‑amino‑6‑N‑hydroxylaminopurine resistance protein |
RA | 4,242,404 | (A)5→4 | intergenic (‑150/+222) | xylE ← / ← malG | D‑xylose:H(+) symporter/maltose ABC transporter membrane subunit MalG |
RA | 4,253,150 | G→A | Q45Q (CAG→CAA) | ubiA → | 4‑hydroxybenzoate octaprenyltransferase |
RA | 4,270,600 | A→G | E121E (GAA→GAG) | yjbQ → | UPF0047 protein YjbQ |
RA | 4,274,964 | T→C | T26T (ACA→ACG) | yjcB ← | uncharacterized protein YjcB |
RA | 4,287,164 | A→G | Y70H (TAC→CAC) | acs ← | acetyl‑CoA synthetase (AMP‑forming) |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
RA | 4,329,894 | (G)6→7 | coding (535/879 nt) | yjcZ → | uncharacterized protein YjcZ |
RA | 4,434,440 | A→G | I110V (ATT→GTT) | ytfH → | putative transcriptional regulator YtfH |
RA | 4,445,549 | A→G | T480A (ACC→GCC) | tamB → | translocation and assembly module subunit TamB |
RA | 4,450,726 | A→G | K255K (AAA→AAG) | ytfQ → | galactofuranose ABC transporter periplasmic binding protein |
RA | 4,468,925 | C→T | A434V (GCC→GTC) | mgtA → | Mg(2(+)) importing P‑type ATPase |
RA | 4,606,094 | (G)8→9 | noncoding (72/87 nt) | leuV ← | tRNA‑Leu |
RA | 4,606,215 | (G)8→9 | noncoding (72/87 nt) | leuP ← | tRNA‑Leu |
RA | 4,610,444 | A→G | D344G (GAC→GGC) | prfC → | peptide chain release factor RF3 |
RA | 4,632,411 | A→G | Y560C (TAC→TGC) | slt → | soluble lytic murein transglycosylase |
RA | 4,633,097 | C→T | intergenic (+11/+136) | trpR → / ← yjjX | DNA‑binding transcriptional repressor TrpR/ITPase/XTPase |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3423770–3424234 | 3424523–3424240 | 7–754 | 18 [11] | [5] 15 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 291401 | NA (NA) | 18 (0.280) | 17/274 | 2.2 | 21.2% | noncoding (1/768 nt) | IS1 | repeat region |
? | NC_000913 | = 4260767 | 67 (1.040) | coding (169/708 nt) | yjbM | uncharacterized protein YjbM | |||||
* | ? | NC_000913 | 1207790 = | 20 (0.310) | 74 (1.310) | 51/242 | 0.1 | 84.7% | coding (290/630 nt) | ycfK | e14 prophage; protein StfP |
? | NC_000913 | 1209619 = | 9 (0.160) | pseudogene (37/537 nt) | stfE | e14 prophage; putative side tail fiber protein fragment | |||||
* | ? | NC_000913 | = 1207805 | 20 (0.310) | 38 (0.670) | 32/242 | 0.6 | 74.0% | coding (305/630 nt) | ycfK | e14 prophage; protein StfP |
? | NC_000913 | = 1209602 | 9 (0.160) | pseudogene (54/537 nt) | stfE | e14 prophage; putative side tail fiber protein fragment | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 46 (0.740) | 29/266 | 0.9 | 100% | intergenic (+253/‑33) | ychE/insH21 | putative inner membrane protein/IS5 transposase and trans‑activator |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 3363001 = | 65 (1.010) | 86 (1.350) | 55/272 | 0.1 | 72.7% | coding (195/2382 nt) | yhcD | putative fimbrial usher protein YhcD |
? | NC_000913 | 3766087 = | 0 (0.000) | coding (3905/4134 nt) | rhsA | rhs element protein RhsA | |||||
* | ? | NC_000913 | = 3653230 | NA (NA) | 72 (1.120) | 49/274 | 0.2 | 97.3% | noncoding (1/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 3766089 | 2 (0.030) | coding (3907/4134 nt) | rhsA | rhs element protein RhsA | |||||
* | ? | NC_000913 | 4542682 = | 30 (0.470) | 23 (0.380) | 18/256 | 1.9 | 41.1% | intergenic (+49/‑433) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | 4542996 = | 38 (0.630) | intergenic (+363/‑119) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit | |||||
* | ? | NC_000913 | = 4542690 | 30 (0.470) | 19 (0.320) | 17/256 | 2.0 | 36.5% | intergenic (+57/‑425) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | = 4542986 | 38 (0.630) | intergenic (+353/‑129) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |