breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 310 (T)9→8 intergenic (+55/‑27) thrL → / → thrA thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1
RA 40,906 A→G R342R (CGT→CGC caiT ← L‑carnitine:gamma‑butyrobetaine antiporter
RA 48,192 A→G T142A (ACG→GCG)  kefC → K(+) : H(+) antiporter KefC
RA 63,248 C→T G6D (GGT→GAT)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
RA 69,104 A→G G315G (GGT→GGC araB ← ribulokinase
RA 194,885 T→C intergenic (+168/‑18) dxr → / → ispU 1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase/undecaprenyl diphosphate synthase
RA 199,200 A→G S425G (AGC→GGC)  bamA → outer membrane protein assembly factor BamA
RA 218,835 (A)8→9 intergenic (‑60/+52) proS ← / ← trmO proline‑‑tRNA ligase/tRNA m(6)t(6)A37 methyltransferase
RA 243,760 A→G N73S (AAC→AGC)  lpcA → D‑sedoheptulose 7‑phosphate isomerase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 301,619 A→G V90A (GTA→GCA)  paoB ← aldehyde dehydrogenase, FAD‑binding subunit
RA 307,306 T→C D676G (GAC→GGC)  ecpC ← putative fimbrial usher protein EcpC
RA 308,054 (C)7→8 coding (1279/2526 nt) ecpC ← putative fimbrial usher protein EcpC
RA 321,564 (G)7→8 intergenic (+483/‑44) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 365,310 A→G G332G (GGT→GGC lacZ ← beta‑galactosidase
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 412,372 A→G I37T (ATT→ACT)  araJ ← putative transport protein AraJ
RA 453,447 A→G G33G (GGT→GGC ampG ← muropeptide:H(+) symporter
RA 483,232 T→C N391S (AAC→AGC)  acrB ← multidrug efflux pump RND permease AcrB
RA 502,496 A→G V248A (GTC→GCC)  ybaL ← putative transporter YbaL
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 527,730 A→G V50V (GTA→GTG ybbC → PF15631 family protein YbbC
RA 552,997 A→G V35A (GTC→GCC)  purE ← N(5)‑carboxyaminoimidazole ribonucleotide mutase
RA 699,536 T→C intergenic (‑359/+38) asnB ← / ← umpH asparagine synthetase B/UMP phosphatase
RA 768,190 C→T D71D (GAC→GAT mngB → alpha‑mannosidase
RA 777,145 (A)5→6 coding (804/1266 nt) tolA → Tol‑Pal system protein TolA
RA 778,662 A→G Q308R (CAG→CGG)  tolB → Tol‑Pal system periplasmic protein TolB
RA 878,840 A→G K33K (AAA→AAG yliI → aldose sugar dehydrogenase YliI
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 922,071 A→G E575E (GAA→GAG macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 945,259 A→G V100A (GTC→GCC)  ycaC ← putative hydrolase
RA 962,213 C→T D73D (GAC→GAT rpsA → 30S ribosomal subunit protein S1
RA 980,123 G→A S1266S (TCG→TCA mukB → chromosome partitioning protein MukB
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,011,593 (A)7→8 coding (1630/1908 nt) uup → ATP‑binding protein with possible role in replication
RA 1,059,436 (G)6→7 coding (181/2547 nt) torA → trimethylamine N‑oxide reductase 1
RA 1,100,097 C→G A153G (GCC→GGC)  ycdY → chaperone protein YcdY
RA 1,106,134 A→G L105L (CTA→CTG ymdB → 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,275,482 C→T L116L (CTG→CTA narL ← DNA‑binding transcriptional dual regulator NarL
RA 1,283,686 (A)5→4 coding (83/1539 nt) narH → nitrate reductase A subunit beta
RA 1,286,570 (T)7→6 intergenic (+44/‑139) narI → / → ychS nitrate reductase A subunit gamma/putative uncharacterized protein YchS
RA 1,291,035 T→C V265A (GTT→GCT)  rssB → regulator of RpoS
RA 1,296,844 A→G G426G (GGT→GGC adhE ← aldehyde‑alcohol dehydrogenase
RA 1,323,030 T→C intergenic (‑84/+8) trpE ← / ← trpL anthranilate synthase subunit TrpE/trp operon leader peptide
JC 1,351,196 (G)7→10 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,398,741 T→C intergenic (‑119/+33) uspE ← / ← fnr universal stress protein with a role cellular motility/DNA‑binding transcriptional dual regulator FNR
RA 1,491,129 A→G K76K (AAA→AAG cybB → cytochrome b561
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,543,407 T→C V218V (GTA→GTG narU ← nitrate/nitrite transporter NarU
RA 1,567,363 A→G intergenic (‑144/+141) yddY ← / ← yddW protein YddY/putative lipoprotein YddW
JC 1,594,132 (C)8→11 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,643,501 T→C T268T (ACA→ACG ydfU ← Qin prophage; protein YdfU
RA 1,660,581 T→C L9P (CTC→CCC)  ynfF → putative selenate reductase YnfF
RA 1,681,163 A→C D63A (GAC→GCC)  folM → dihydromonapterin reductase
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,746,014 G→A G38G (GGC→GGT ydhQ ← adhesin‑like autotransporter YdhQ
RA 1,849,310 Δ1 bp coding (474/1857 nt) sppA → protease IV, a signal peptide peptidase
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,875,056 (C)6→7 coding (521/822 nt) nimR ← DNA‑binding transcriptional repressor NimR
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,885,910 A→G K22K (AAA→AAG yeaX → carnitine monooxygenase subunit YeaX
RA 1,921,492 T→C W424R (TGG→CGG)  rsmF → 16S rRNA m(5)C1407 methyltransferase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,008,322 G→C A16P (GCC→CCC)  yedE → putative selenium transporter YedE
RA 2,123,008 G→A intergenic (‑29/+76) cpsG ← / ← cpsB phosphomannomutase/mannose‑1‑phosphate guanylyltransferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,188,193 T→C G74G (GGA→GGG yehA ← putative fimbrial adhesin YehA
RA 2,274,432 T→C V85A (GTG→GCG)  yejB → putative oligopeptide ABC transporter membrane subunit YejB
RA 2,339,970 T→C V135A (GTC→GCC)  ubiG → bifunctional 3‑demethylubiquinone‑8 3‑O‑methyltransferase and 2‑octaprenyl‑6‑hydroxyphenol methylase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,592,616 T→C V337A (GTG→GCG)  dapE → succinyl‑diaminopimelate desuccinylase
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,603,074 T→C R419R (CGT→CGC hyfB → hydrogenase 4 component B
RA 2,630,822 T→C V166A (GTG→GCG)  yfgI → nalidixic acid resistance protein YfgI
RA 2,644,674 A→G V64A (GTT→GCT)  ndk ← nucleoside diphosphate kinase
RA 2,687,452 T→C intergenic (‑44/+17) glnB ← / ← glrR nitrogen regulatory protein PII‑1/DNA‑binding transcriptional activator GlrR
RA 2,731,729 A→G R815R (CGT→CGC clpB ← ClpB chaperone
RA 2,734,543 (C)8→9 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,740,439 Δ1 bp coding (712/1071 nt) aroF ← 3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive
RA 2,799,453 T→C V97A (GTG→GCG)  alaE → L‑alanine exporter
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,824,478 (C)6→7 intergenic (‑132/+13) pncC ← / ← mltB NMN aminohydrolase/membrane‑bound lytic murein transglycosylase B
RA 2,839,731 A→G T70A (ACC→GCC)  ascF → beta‑glucoside specific PTS enzyme IIBC component
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,883,287 A→G C284C (TGT→TGC casA ← type I‑E CRISPR system Cascade subunit CasA
RA 2,897,937 Δ1 bp intergenic (‑67/+27) ygcS ← / ← ygcU putative transporter YgcS/putative FAD‑containing dehydrogenase
RA 2,906,780 C→T A388T (GCT→ACT)  eno ← enolase
RA 2,910,916 G→A P59L (CCG→CTG)  mazF ← endoribonuclease toxin MazF
RA 2,920,095 T→C T218A (ACT→GCT)  gudX ← glucarate dehydratase‑related protein
RA 2,942,553 T→C L5L (TTA→TTG gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,968,911 T→C M23V (ATG→GTG)  rppH ← RNA pyrophosphohydrolase
RA 2,978,049 A→G V284A (GTG→GCG)  lysA ← diaminopimelate decarboxylase
RA 2,978,378 T→C A174A (GCA→GCG lysA ← diaminopimelate decarboxylase
RA 2,994,310 G→A pseudogene (372/503 nt) pbl → transglycosylase domain‑containing protein Pbl
RA 2,999,680 (G)7→8 coding (212/756 nt) ygeR ← LysM domain‑containing putative peptidase lipoprotein YgeR
RA 3,039,599 T→C T49A (ACG→GCG)  xerD ← site‑specific recombinase
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,151,539 A→G V149A (GTC→GCC)  exbB ← Ton complex subunit ExbB
RA 3,192,793 A→G T196A (ACG→GCG)  yqiJ → putative inner membrane protein
RA 3,230,773 T→C V629A (GTA→GCA)  ygjK → glycoside hydrolase
RA 3,252,614 +A coding (311/366 nt) yhaH → putative inner membrane protein
RA 3,274,206 T→C A234A (GCA→GCG garP ← galactarate/glucarate/glycerate transporter GarP
RA 3,292,985 C→T V117V (GTG→GTA rsmI ← 16S rRNA 2'‑O‑ribose C1402 methyltransferase
RA 3,305,589 T→C T77A (ACC→GCC)  mtr ← tryptophan:H(+) symporter Mtr
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,354,895 G→A L57L (TTG→TTA gltB → glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,456,195 A→G L206L (CTA→CTG gspC → Type II secretion system protein GspC
RA 3,499,074 A→G Q416R (CAG→CGG)  cysG → siroheme synthase
RA 3,521,612 T→C T121A (ACG→GCG)  hofN ← DNA utilization protein HofN
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,640,471 T→C S121P (TCT→CCT)  uspA → universal stress global stress response regulator
RA 3,658,602 A→G K79K (AAA→AAG gadE → DNA‑binding transcriptional activator GadE
RA 3,664,363 (G)6→7 coding (256/729 nt) gadW ← DNA‑binding transcriptional dual regulator GadW
RA 3,728,118 (C)8→7 coding (1254/1455 nt) xylB ← xylulokinase
RA 3,779,008 T→C V537A (GTG→GCG)  lldP → lactate/glycolate:H(+) symporter LldP
RA 3,782,359 T→C E68E (GAA→GAG cysE ← serine acetyltransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,834,428 (G)6→7 coding (110/2319 nt) yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,863,987 (A)7→8 coding (89/717 nt) yidP → putative DNA‑binding transcriptional regulator YidP
RA 3,870,267 T→C R297R (CGT→CGC cbrA → colicin M resistance protein
RA 3,874,882 C→T R93R (CGG→CGA dgoR ← putative DNA‑binding transcriptional regulator DgoR
RA 3,894,975 T→C G108G (GGT→GGC yieF → chromate reductase
RA 3,961,491 A→G K272R (AAG→AGG)  rep → ATP‑dependent DNA helicase Rep
RA 3,969,767 T→C V246A (GTA→GCA)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,996,724 T→C G146G (GGT→GGC xerC → site‑specific tyrosine recombinase
RA 4,027,317 A→G intergenic (+89/+292) fre → / ← fadA flavin reductase/3‑ketoacyl‑CoA thiolase
RA 4,050,693 T→C T25A (ACC→GCC)  yihA ← GTP‑binding protein YihA
RA 4,062,890 T→C V215A (GTG→GCG)  yihN → putative transporter YihN
RA 4,086,523 A→G T170A (ACT→GCT)  fdhD → sulfurtransferase for molybdenum cofactor sulfuration
RA 4,103,043 A→G E74E (GAA→GAG yiiM → 2‑amino‑6‑N‑hydroxylaminopurine resistance protein
RA 4,242,404 (A)5→4 intergenic (‑150/+222) xylE ← / ← malG D‑xylose:H(+) symporter/maltose ABC transporter membrane subunit MalG
RA 4,253,150 G→A Q45Q (CAG→CAA ubiA → 4‑hydroxybenzoate octaprenyltransferase
RA 4,270,600 A→G E121E (GAA→GAG yjbQ → UPF0047 protein YjbQ
RA 4,274,964 T→C T26T (ACA→ACG yjcB ← uncharacterized protein YjcB
RA 4,287,164 A→G Y70H (TAC→CAC)  acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,329,894 (G)6→7 coding (535/879 nt) yjcZ → uncharacterized protein YjcZ
RA 4,434,440 A→G I110V (ATT→GTT)  ytfH → putative transcriptional regulator YtfH
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,450,726 A→G K255K (AAA→AAG ytfQ → galactofuranose ABC transporter periplasmic binding protein
RA 4,468,925 C→T A434V (GCC→GTC)  mgtA → Mg(2(+)) importing P‑type ATPase
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,610,444 A→G D344G (GAC→GGC)  prfC → peptide chain release factor RF3
RA 4,632,411 A→G Y560C (TAC→TGC)  slt → soluble lytic murein transglycosylase
RA 4,633,097 C→T intergenic (+11/+136) trpR → / ← yjjX DNA‑binding transcriptional repressor TrpR/ITPase/XTPase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423770–3424234 3424523–3424240 7–754 18 [11] [5] 15 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 291401NA (NA)18 (0.280) 17/274 2.2 21.2% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 4260767 67 (1.040)coding (169/708 nt) yjbM uncharacterized protein YjbM
* ? NC_000913 1207790 =20 (0.310)74 (1.310) 51/242 0.1 84.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 9 (0.160)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780520 (0.310)38 (0.670) 32/242 0.6 74.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 9 (0.160)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)46 (0.740) 29/266 0.9 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =65 (1.010)86 (1.350) 55/272 0.1 72.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)72 (1.120) 49/274 0.2 97.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =30 (0.470)23 (0.380) 18/256 1.9 41.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 38 (0.630)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269030 (0.470)19 (0.320) 17/256 2.0 36.5% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 38 (0.630)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit