| ALE replicate | Flask count | Isolate count |
|---|---|---|
| 0 | 1 | 1 |
| 1 | 1 | 1 |
| 2 | 1 | 1 |
| 3 | 1 | 1 |
| 4 | 1 | 1 |
| 5 | 1 | 1 |
| 6 | 1 | 1 |
| 7 | 1 | 1 |
| 8 | 1 | 1 |
| 9 | 1 | 1 |
| 10 | 1 | 1 |
| 11 | 1 | 1 |
| 12 | 1 | 1 |
| 13 | 1 | 1 |
| 14 | 1 | 1 |
| 15 | 1 | 1 |
| 16 | 1 | 1 |
| 17 | 1 | 1 |
| 18 | 1 | 1 |
| 20 | 1 | 1 |
| 21 | 1 | 1 |
| 22 | 1 | 1 |
| 23 | 1 | 1 |
| 24 | 1 | 1 |
| 25 | 1 | 1 |
| 26 | 1 | 1 |
| 27 | 1 | 1 |
| 28 | 1 | 1 |
| 31 | 1 | 1 |
| 32 | 1 | 1 |
| 33 | 1 | 1 |
| 34 | 1 | 1 |
| 35 | 1 | 1 |
| 38 | 1 | 1 |
| 39 | 1 | 1 |
| 40 | 1 | 1 |
| 41 | 1 | 1 |
| 42 | 1 | 1 |
| 43 | 1 | 1 |
| 44 | 1 | 1 |
| 45 | 1 | 1 |
| 46 | 1 | 1 |
| 47 | 1 | 1 |
| 48 | 1 | 1 |
| 51 | 1 | 1 |
| 52 | 1 | 1 |
| 53 | 1 | 1 |
| 54 | 1 | 1 |
| 55 | 1 | 1 |
| 56 | 1 | 1 |
| 57 | 1 | 1 |
| 58 | 1 | 1 |
| 59 | 1 | 1 |
| 60 | 1 | 1 |
| 61 | 1 | 1 |
| 64 | 1 | 1 |
| 65 | 1 | 1 |
| 66 | 1 | 1 |
| 67 | 1 | 1 |
| 68 | 1 | 1 |
| 69 | 1 | 1 |
| 70 | 1 | 1 |
| 71 | 1 | 1 |
| 72 | 1 | 1 |
| 73 | 1 | 1 |
| 74 | 1 | 1 |
| 75 | 1 | 1 |
| 76 | 1 | 1 |
| 77 | 1 | 1 |
| 78 | 1 | 1 |
| 79 | 1 | 1 |
| 80 | 1 | 1 |
| 81 | 1 | 1 |
| 82 | 1 | 1 |
| 83 | 1 | 1 |
| 84 | 1 | 1 |
| 85 | 1 | 1 |
| 86 | 1 | 1 |
| 87 | 1 | 1 |
| 89 | 1 | 1 |
| 91 | 1 | 1 |
| 92 | 1 | 1 |
| 93 | 1 | 1 |
| 94 | 1 | 1 |
| 95 | 1 | 1 |
| 97 | 1 | 1 |
| 101 | 1 | 1 |
| 105 | 1 | 1 |
| 106 | 1 | 1 |
| 107 | 1 | 1 |
| 108 | 1 | 1 |
| 110 | 1 | 1 |
| 112 | 1 | 1 |
| 114 | 1 | 1 |
| 118 | 1 | 1 |
| 119 | 1 | 1 |
| 120 | 1 | 1 |
| 122 | 1 | 1 |
| 124 | 1 | 1 |
| 126 | 1 | 1 |
| 127 | 1 | 1 |
| 130 | 1 | 1 |
| 131 | 1 | 1 |
| 132 | 1 | 1 |
| 133 | 1 | 1 |
| 134 | 1 | 1 |
| 135 | 1 | 1 |
| 136 | 1 | 1 |
| 137 | 1 | 1 |
| 138 | 1 | 1 |
| 139 | 1 | 1 |
| 142 | 1 | 1 |
| 143 | 1 | 1 |
| 113 | 113 | 113 |
| Mutation Type | Unique | Observed |
|---|---|---|
| Single Base Substitutions | 690 | 1153 |
| Multiple Base Substitutions | 4 | 4 |
| Deletions | 172 | 343 |
| Insertions | 70 | 313 |
| Mobile Element Insertions | 46 | 172 |
| Amplifications | 0 | 0 |
| Gene Conversions | 0 | 0 |
| Inversions | 0 | 0 |
| Total | 982 | 1985 |
| ALE, Flask, Isolate | Predicted Mutations | Unassigned Missing Coverage Evidence | Mean Coverage | Total Reads | Percent Mapped | Mapped Reads | Average Read Length |
|---|---|---|---|---|---|---|---|
| A0 F1 I1 R1 | 7 | 0 | 175.1 | 22577466 | 95.2% | 21493747 | 37.0 |
| A1 F1 I1 R1 | 17 | 0 | 37.0 | 2377229 | 96.2% | 2286894 | 75.9 |
| A2 F1 I1 R1 | 14 | 0 | 24.7 | 1576304 | 96.5% | 1521133 | 75.8 |
| A3 F1 I1 R1 | 18 | 0 | 29.2 | 1933165 | 95.1% | 1838439 | 75.7 |
| A4 F1 I1 R1 | 15 | 0 | 35.6 | 2322208 | 94.1% | 2185197 | 75.8 |
| A5 F1 I1 R1 | 19 | 0 | 34.1 | 2156969 | 96.4% | 2079318 | 75.9 |
| A6 F1 I1 R1 | 20 | 0 | 51.0 | 3438118 | 91.8% | 3156192 | 75.9 |
| A7 F1 I1 R1 | 20 | 0 | 54.5 | 3547384 | 95.1% | 3373562 | 76.0 |
| A8 F1 I1 R1 | 15 | 0 | 61.9 | 4096487 | 94.5% | 3871180 | 75.9 |
| A9 F1 I1 R1 | 16 | 0 | 55.7 | 3673927 | 94.4% | 3468187 | 75.9 |
| A10 F1 I1 R1 | 14 | 0 | 23.9 | 1506608 | 98.1% | 1477982 | 76.0 |
| A11 F1 I1 R1 | 21 | 0 | 62.2 | 4089897 | 94.3% | 3856772 | 75.9 |
| A12 F1 I1 R1 | 18 | 0 | 38.4 | 2430560 | 95.7% | 2326045 | 75.9 |
| A13 F1 I1 R1 | 17 | 0 | 44.3 | 2973330 | 95.1% | 2827636 | 75.9 |
| A14 F1 I1 R1 | 17 | 0 | 21.6 | 1356596 | 98.4% | 1334890 | 76.0 |
| A15 F1 I1 R1 | 14 | 0 | 43.4 | 2705329 | 98.7% | 2670159 | 76.0 |
| A16 F1 I1 R1 | 16 | 0 | 38.1 | 2332770 | 98.7% | 2302443 | 76.0 |
| A17 F1 I1 R1 | 15 | 0 | 26.4 | 1686296 | 95.9% | 1617157 | 76.0 |
| A18 F1 I1 R1 | 18 | 0 | 50.6 | 3235256 | 96.4% | 3118786 | 76.0 |
| A20 F1 I1 R1 | 17 | 0 | 44.7 | 2849266 | 96.8% | 2758089 | 76.0 |
| A21 F1 I1 R1 | 16 | 0 | 43.9 | 3070277 | 96.2% | 2953606 | 75.9 |
| A22 F1 I1 R1 | 17 | 0 | 44.7 | 2806446 | 96.5% | 2708220 | 76.0 |
| A23 F1 I1 R1 | 14 | 0 | 40.5 | 2605368 | 96.4% | 2511574 | 75.9 |
| A24 F1 I1 R1 | 18 | 0 | 37.8 | 2419812 | 96.5% | 2335118 | 76.0 |
| A25 F1 I1 R1 | 14 | 0 | 46.3 | 3075166 | 96.7% | 2973685 | 76.0 |
| A26 F1 I1 R1 | 14 | 0 | 45.0 | 2861610 | 96.8% | 2770038 | 76.0 |
| A27 F1 I1 R1 | 17 | 0 | 37.2 | 2333115 | 96.5% | 2251455 | 75.9 |
| A28 F1 I1 R1 | 15 | 0 | 45.6 | 2893554 | 96.7% | 2798066 | 76.0 |
| A31 F1 I1 R1 | 21 | 0 | 37.9 | 2424921 | 96.5% | 2340048 | 76.0 |
| A32 F1 I1 R1 | 13 | 0 | 41.3 | 2638873 | 95.5% | 2520123 | 75.9 |
| A33 F1 I1 R1 | 22 | 0 | 54.4 | 3455062 | 95.8% | 3309949 | 75.9 |
| A34 F1 I1 R1 | 18 | 0 | 56.1 | 3547650 | 96.9% | 3437672 | 76.0 |
| A35 F1 I1 R1 | 21 | 0 | 46.3 | 3071142 | 93.0% | 2856162 | 75.9 |
| A38 F1 I1 R1 | 17 | 0 | 54.1 | 3391312 | 97.1% | 3292963 | 75.9 |
| A39 F1 I1 R1 | 15 | 0 | 59.3 | 3695894 | 97.1% | 3588713 | 75.9 |
| A40 F1 I1 R1 | 16 | 0 | 59.7 | 3732628 | 97.2% | 3628114 | 76.0 |
| A41 F1 I1 R1 | 21 | 0 | 57.1 | 3643130 | 97.0% | 3533836 | 75.9 |
| A42 F1 I1 R1 | 17 | 0 | 53.4 | 3386926 | 97.4% | 3298865 | 76.0 |
| A43 F1 I1 R1 | 17 | 0 | 61.8 | 3886062 | 97.4% | 3785024 | 76.0 |
| A44 F1 I1 R1 | 19 | 0 | 54.5 | 3474856 | 97.7% | 3394934 | 76.0 |
| A45 F1 I1 R1 | 14 | 0 | 53.1 | 3307936 | 97.7% | 3231853 | 76.0 |
| A46 F1 I1 R1 | 17 | 0 | 71.0 | 4433184 | 98.0% | 4344520 | 76.0 |
| A47 F1 I1 R1 | 17 | 0 | 69.1 | 4502028 | 96.3% | 4335452 | 75.9 |
| A48 F1 I1 R1 | 19 | 0 | 65.6 | 4133022 | 97.0% | 4009031 | 76.0 |
| A51 F1 I1 R1 | 20 | 0 | 58.0 | 3778966 | 96.2% | 3635365 | 75.9 |
| A52 F1 I1 R1 | 19 | 0 | 66.0 | 4263516 | 97.2% | 4144137 | 76.0 |
| A53 F1 I1 R1 | 17 | 0 | 63.8 | 4051746 | 97.3% | 3942348 | 76.0 |
| A54 F1 I1 R1 | 16 | 0 | 53.7 | 3449390 | 96.4% | 3325211 | 75.9 |
| A55 F1 I1 R1 | 18 | 0 | 62.6 | 3986840 | 96.5% | 3847300 | 75.9 |
| A56 F1 I1 R1 | 16 | 0 | 62.6 | 4005459 | 97.2% | 3893306 | 75.9 |
| A57 F1 I1 R1 | 16 | 0 | 65.9 | 4148770 | 97.1% | 4028455 | 75.9 |
| A58 F1 I1 R1 | 18 | 0 | 55.3 | 3756050 | 96.8% | 3635856 | 75.9 |
| A59 F1 I1 R1 | 21 | 0 | 55.8 | 3542164 | 96.7% | 3425272 | 76.0 |
| A60 F1 I1 R1 | 14 | 0 | 61.1 | 3976207 | 96.5% | 3837039 | 76.0 |
| A61 F1 I1 R1 | 19 | 0 | 50.7 | 3232285 | 96.8% | 3128851 | 74.9 |
| A64 F1 I1 R1 | 17 | 0 | 58.5 | 3743480 | 96.8% | 3623688 | 76.0 |
| A65 F1 I1 R1 | 19 | 0 | 67.5 | 4307580 | 97.2% | 4186967 | 74.9 |
| A66 F1 I1 R1 | 19 | 0 | 57.4 | 3651244 | 97.1% | 3545357 | 76.0 |
| A67 F1 I1 R1 | 17 | 0 | 65.0 | 4212288 | 95.3% | 4014310 | 75.9 |
| A68 F1 I1 R1 | 17 | 0 | 56.4 | 3914180 | 96.2% | 3765441 | 75.9 |
| A69 F1 I1 R1 | 20 | 0 | 59.3 | 3764421 | 95.9% | 3610079 | 75.9 |
| A70 F1 I1 R1 | 18 | 0 | 48.6 | 3152226 | 95.8% | 3019832 | 76.0 |
| A71 F1 I1 R1 | 18 | 0 | 59.4 | 3821164 | 95.9% | 3664496 | 75.9 |
| A72 F1 I1 R1 | 16 | 0 | 60.6 | 3810144 | 97.0% | 3695839 | 75.8 |
| A73 F1 I1 R1 | 21 | 0 | 62.0 | 3993596 | 96.1% | 3837845 | 76.0 |
| A74 F1 I1 R1 | 16 | 0 | 49.9 | 3507628 | 96.2% | 3374338 | 75.9 |
| A75 F1 I1 R1 | 16 | 0 | 50.8 | 3316832 | 95.7% | 3174208 | 75.9 |
| A76 F1 I1 R1 | 13 | 0 | 52.2 | 3332858 | 96.5% | 3216207 | 76.0 |
| A77 F1 I1 R1 | 21 | 0 | 53.0 | 3608474 | 95.6% | 3449701 | 76.0 |
| A78 F1 I1 R1 | 16 | 0 | 49.7 | 3184232 | 95.8% | 3050494 | 76.0 |
| A79 F1 I1 R1 | 19 | 0 | 52.0 | 3333102 | 95.7% | 3189778 | 75.9 |
| A80 F1 I1 R1 | 17 | 0 | 48.4 | 3161007 | 95.9% | 3031405 | 76.0 |
| A81 F1 I1 R1 | 13 | 0 | 64.8 | 4113440 | 96.3% | 3961242 | 75.9 |
| A82 F1 I1 R1 | 16 | 0 | 52.8 | 3323890 | 97.1% | 3227497 | 76.0 |
| A83 F1 I1 R1 | 17 | 0 | 39.7 | 2446701 | 98.6% | 2412447 | 76.0 |
| A84 F1 I1 R1 | 17 | 0 | 47.9 | 3074446 | 96.0% | 2951468 | 76.0 |
| A85 F1 I1 R1 | 15 | 0 | 51.9 | 3515760 | 95.5% | 3357550 | 75.9 |
| A86 F1 I1 R1 | 15 | 0 | 50.6 | 3276358 | 95.9% | 3142027 | 75.9 |
| A87 F1 I1 R1 | 13 | 0 | 46.2 | 2986023 | 95.8% | 2860610 | 75.9 |
| A89 F1 I1 R1 | 15 | 0 | 55.0 | 3646496 | 95.5% | 3482403 | 75.9 |
| A91 F1 I1 R1 | 18 | 0 | 55.4 | 3661525 | 95.1% | 3482110 | 76.0 |
| A92 F1 I1 R1 | 15 | 0 | 58.2 | 3997918 | 96.1% | 3841999 | 76.0 |
| A93 F1 I1 R1 | 15 | 0 | 55.4 | 3682408 | 94.4% | 3476193 | 75.9 |
| A94 F1 I1 R1 | 15 | 0 | 48.6 | 3202402 | 95.4% | 3055091 | 75.9 |
| A95 F1 I1 R1 | 16 | 0 | 38.4 | 2546724 | 96.0% | 2444855 | 75.9 |
| A97 F1 I1 R1 | 18 | 0 | 21.9 | 1431356 | 95.6% | 1368376 | 76.0 |
| A101 F1 I1 R1 | 20 | 0 | 160.9 | 10398134 | 92.8% | 9649468 | 75.9 |
| A105 F1 I1 R1 | 16 | 0 | 277.3 | 18051348 | 92.9% | 16769702 | 75.9 |
| A106 F1 I1 R1 | 16 | 0 | 286.4 | 19211214 | 90.7% | 17424571 | 75.8 |
| A107 F1 I1 R1 | 19 | 0 | 326.9 | 20855348 | 93.1% | 19416328 | 75.9 |
| A108 F1 I1 R1 | 80 | 0 | 195.1 | 12639756 | 93.3% | 11792892 | 75.9 |
| A110 F1 I1 R1 | 14 | 0 | 278.2 | 17968888 | 92.5% | 16621221 | 75.9 |
| A112 F1 I1 R1 | 17 | 0 | 249.1 | 16570612 | 93.0% | 15410669 | 76.0 |
| A114 F1 I1 R1 | 20 | 0 | 239.5 | 15463816 | 93.1% | 14396812 | 76.0 |
| A118 F1 I1 R1 | 15 | 0 | 203.6 | 13305188 | 92.8% | 12347214 | 75.9 |
| A119 F1 I1 R1 | 20 | 0 | 247.5 | 15984500 | 92.7% | 14817631 | 75.9 |
| A120 F1 I1 R1 | 20 | 0 | 248.8 | 16334864 | 93.0% | 15191423 | 76.0 |
| A122 F1 I1 R1 | 27 | 0 | 151.2 | 10056804 | 92.4% | 9292486 | 76.0 |
| A124 F1 I1 R1 | 18 | 0 | 228.8 | 14151462 | 95.4% | 13500494 | 76.0 |
| A126 F1 I1 R1 | 14 | 0 | 223.8 | 14402144 | 95.1% | 13696438 | 76.0 |
| A127 F1 I1 R1 | 15 | 0 | 330.5 | 20417406 | 95.5% | 19498622 | 76.0 |
| A130 F1 I1 R1 | 13 | 0 | 250.2 | 15681636 | 95.5% | 14975962 | 76.0 |
| A131 F1 I1 R1 | 13 | 0 | 244.3 | 15300636 | 95.6% | 14627408 | 76.0 |
| A132 F1 I1 R1 | 21 | 0 | 265.3 | 16744490 | 95.4% | 15974243 | 76.0 |
| A133 F1 I1 R1 | 21 | 0 | 238.6 | 15028552 | 95.5% | 14352267 | 76.0 |
| A134 F1 I1 R1 | 15 | 0 | 220.5 | 13917488 | 95.6% | 13305118 | 76.0 |
| A135 F1 I1 R1 | 14 | 0 | 200.0 | 12709596 | 94.6% | 12023277 | 76.0 |
| A136 F1 I1 R1 | 21 | 0 | 250.8 | 15742942 | 95.7% | 15065995 | 76.0 |
| A137 F1 I1 R1 | 18 | 0 | 221.5 | 13936938 | 95.6% | 13323712 | 76.0 |
| A138 F1 I1 R1 | 17 | 0 | 163.5 | 10262604 | 95.7% | 9821312 | 76.0 |
| A139 F1 I1 R1 | 15 | 0 | 48.9 | 3081653 | 97.3% | 2998448 | 76.0 |
| A142 F1 I1 R1 | 21 | 0 | 42.6 | 2691336 | 96.3% | 2591756 | 76.0 |
| A143 F1 I1 R1 | 16 | 0 | 39.8 | 2536279 | 96.2% | 2439900 | 76.0 |