breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 3,827,189 Δ438 bp coding (385‑822/1020 nt) ptxS ← 2‑ketogluconate utilization repressor
RA 4,717,498 C→G A254G (GCC→GGC)  PP_4173 → two‑component system sensor histidine kinase/response regulator
RA 4,743,195 +C intergenic (+464/+288) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,197 A→G intergenic (+466/+286) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,199 C→T intergenic (+468/+284) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,202 C→T intergenic (+471/+281) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,205 C→G intergenic (+474/+278) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,207 2 bp→CT intergenic (+476/+275) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,221 C→T intergenic (+490/+262) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,223 A→C intergenic (+492/+260) gltA → / ← yeiW citrate synthase/putative oxidoreductase
RA 4,743,226 2 bp→GC intergenic (+495/+256) gltA → / ← yeiW citrate synthase/putative oxidoreductase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_002947 171395–172965 176268–172972 8–4874 3 [1] [1] 2 [PP_16SA]–[PP_5SA] [PP_16SA],PP_23SA,[PP_5SA]
* * ÷ NC_002947 176688–178395 181689–178402 8–5002 3 [1] [1] 3 PP_16SB–[PP_r01] PP_16SB,PP_23SB,[PP_r01]
* * ÷ NC_002947 4552100 4552174 75 2 [1] [1] 2 pydX NADP‑dependent dihydropyrimidine dehydrogenase subunit
* * ÷ NC_002947 6183852 6183858 7 25 [0] [0] NA atpI/– ATP synthase protein I/–

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_002947 1 =0 (0.000)25 (1.500) 21/286 0.0 100% intergenic (–/+146) –/parB –/chromosome‑partitioning protein
?NC_002947 = 6183851 0 (0.000)intergenic (‑3/–) atpI/– ATP synthase protein I/–
* ? NC_002947 139996 =12 (0.720)3 (0.190) 3/276 1.8 21.0% coding (508/1782 nt) kinB alginate biosynthesis sensor protein KinB
?NC_002947 140016 = 11 (0.680)coding (488/1782 nt) kinB alginate biosynthesis sensor protein KinB
* ? NC_002947 164102 =12 (0.720)3 (0.180) 3/280 1.9 22.4% coding (1355/1437 nt) pntB pyridine nucleotide transhydrogenase subunit beta
?NC_002947 164137 = 9 (0.550)coding (1320/1437 nt) pntB pyridine nucleotide transhydrogenase subunit beta
* ? NC_002947 274147 =5 (0.300)3 (0.180) 3/282 1.9 37.7% coding (423/801 nt) PP_0221 methionine ABC transporter substrate‑binding protein
?NC_002947 274172 = 5 (0.300)coding (398/801 nt) PP_0221 methionine ABC transporter substrate‑binding protein
* ? NC_002947 298218 =16 (0.960)4 (0.250) 4/278 1.6 20.2% coding (324/384 nt) PP_0244 hypothetical protein
?NC_002947 298244 = 16 (0.990)coding (298/384 nt) PP_0244 hypothetical protein
* ? NC_002947 = 35338115 (0.900)4 (0.250) 4/278 1.6 21.3% coding (952/1179 nt) cbcV choline/betaine/carnitine ABC transporter ATP binding protein
?NC_002947 = 353398 15 (0.930)coding (935/1179 nt) cbcV choline/betaine/carnitine ABC transporter ATP binding protein
* ? NC_002947 401360 =12 (0.720)3 (0.200) 3/260 1.7 25.1% coding (943/1059 nt) PP_0334 transposase
?NC_002947 3970871 = 7 (0.460)coding (1033/1179 nt) PP_3498 transposase
* ? NC_002947 = 41362012 (0.720)3 (0.190) 3/272 1.8 20.4% coding (2571/2934 nt) glnE glutamate‑ammonia‑ligase adenylyltransferase
?NC_002947 = 413635 12 (0.760)coding (2586/2934 nt) glnE glutamate‑ammonia‑ligase adenylyltransferase
* ? NC_002947 511871 =15 (0.900)4 (0.250) 4/270 1.5 21.5% intergenic (+8/‑140) trpC/PP_0423 indole‑3‑glycerol phosphate synthase/hypothetical protein
?NC_002947 511893 = 15 (0.950)intergenic (+30/‑118) trpC/PP_0423 indole‑3‑glycerol phosphate synthase/hypothetical protein
* ? NC_002947 = 69566912 (0.720)4 (0.250) 4/270 1.5 27.3% coding (87/1494 nt) mmsA‑I methylmalonate‑semialdehyde dehydrogenase
?NC_002947 = 695687 10 (0.640)coding (105/1494 nt) mmsA‑I methylmalonate‑semialdehyde dehydrogenase
* ? NC_002947 732787 =1 (0.060)3 (0.190) 3/274 1.8 75.4% coding (2324/2565 nt) clpB chaperone protein ClpB
?NC_002947 732821 = 1 (0.060)coding (2358/2565 nt) clpB chaperone protein ClpB
* ? NC_002947 = 86792118 (1.080)5 (0.310) 4/280 1.6 21.0% intergenic (+16/+161) PP_0750/mqo‑I hypothetical protein/malate:quinone oxidoreductase
?NC_002947 = 868013 20 (1.220)intergenic (+108/+69) PP_0750/mqo‑I hypothetical protein/malate:quinone oxidoreductase
* ? NC_002947 = 9142158 (0.480)6 (0.380) 6/274 1.1 34.6% coding (923/1743 nt) fruA PTS fructose transporter subunit IIBC
?NC_002947 = 914241 15 (0.940)coding (949/1743 nt) fruA PTS fructose transporter subunit IIBC
* ? NC_002947 1076410 =13 (0.780)3 (0.190) 3/274 1.8 21.9% intergenic (+4/‑217) mreB/mreC cell wall structural actin‑like protein MreB/cell shape‑determining protein MreC
?NC_002947 1076429 = 9 (0.560)intergenic (+23/‑198) mreB/mreC cell wall structural actin‑like protein MreB/cell shape‑determining protein MreC
* ? NC_002947 = 1426821NA (NA)5 (0.310) 5/276 1.3 NA coding (712/1422 nt) PP_1250 group II intron‑encoding maturase
?NC_002947 = 1426852 NA (NA)coding (743/1422 nt) PP_1250 group II intron‑encoding maturase
* ? NC_002947 1436025 =10 (0.600)4 (0.250) 4/270 1.5 37.3% intergenic (‑55/+98) PP_1256/PP_1257 alpha‑ketoglutarate semialdehyde dehydrogenase/1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase
?NC_002947 1436102 = 4 (0.250)intergenic (‑132/+21) PP_1256/PP_1257 alpha‑ketoglutarate semialdehyde dehydrogenase/1‑pyrroline‑4‑hydroxy‑2‑carboxylate deaminase
* ? NC_002947 = 152670711 (0.660)4 (0.260) 3/268 1.8 24.8% coding (852/957 nt) ddlB D‑alanine‑‑D‑alanine ligase B
?NC_002947 = 1526720 14 (0.900)coding (865/957 nt) ddlB D‑alanine‑‑D‑alanine ligase B
* ? NC_002947 1762884 =14 (0.840)3 (0.190) 3/276 1.8 20.3% coding (476/477 nt) PP_1569 hypothetical protein
?NC_002947 1762915 = 10 (0.620)coding (31/342 nt) PP_1570 head‑tail adaptor
* ? NC_002947 = 17801272 (0.120)8 (0.550) 7/248 0.8 77.2% intergenic (+44/+62) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
?NC_002947 = 1780151 3 (0.210)intergenic (+68/+38) PP_1587/dapC NhaP‑type Na+(K+)/H+ antiporter/N‑succinyl‑L,L‑diaminopimelate aminotransferase
* ? NC_002947 1798642 =16 (0.960)5 (0.310) 5/276 1.3 24.7% coding (523/1128 nt) lpxB lipid‑A‑disaccharide synthase
?NC_002947 1798676 = 15 (0.930)coding (557/1128 nt) lpxB lipid‑A‑disaccharide synthase
* ? NC_002947 1811061 =14 (0.840)3 (0.190) 3/270 1.8 22.1% coding (334/708 nt) ispD 2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase
?NC_002947 1811088 = 8 (0.510)coding (361/708 nt) ispD 2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase
* ? NC_002947 = 18210270 (0.000)3 (0.190) 3/276 1.8 100% coding (748/1422 nt) PP_1624 group II intron‑encoding maturase
?NC_002947 = 2072298 NA (NA)coding (707/1422 nt) PP_1846 group II intron‑encoding maturase
* ? NC_002947 2050759 =8 (0.480)4 (0.270) 3/252 1.7 44.3% intergenic (+33/+106) PP_1821/PP_1822 glutathione S‑transferase family protein/hypothetical protein
?NC_002947 2050850 = 3 (0.200)intergenic (+124/+15) PP_1821/PP_1822 glutathione S‑transferase family protein/hypothetical protein
* ? NC_002947 = 205256011 (0.660)5 (0.400) 3/212 1.4 33.2% intergenic (‑88/+87) PP_1824/PP_1825 Smr domain‑containing protein/hypothetical protein
?NC_002947 = 2052596 12 (0.970)intergenic (‑124/+51) PP_1824/PP_1825 Smr domain‑containing protein/hypothetical protein
* ? NC_002947 = 205794313 (0.780)4 (0.270) 4/254 1.4 30.1% intergenic (+27/‑116) aroC/PP_1831 chorismate synthase/membrane protein
?NC_002947 = 2058017 7 (0.470)intergenic (+101/‑42) aroC/PP_1831 chorismate synthase/membrane protein
* ? NC_002947 = 207314411 (0.660)4 (0.270) 4/250 1.4 29.0% intergenic (+131/+96) PP_1846/PP_1847 group II intron‑encoding maturase/TonB‑dependent ferric siderophore receptor
?NC_002947 = 2073181 10 (0.690)intergenic (+168/+59) PP_1846/PP_1847 group II intron‑encoding maturase/TonB‑dependent ferric siderophore receptor
* ? NC_002947 = 2088215NA (NA)3 (0.190) 3/270 1.8 NA coding (365/1302 nt) PP_1865 transposase
?NC_002947 = 2088261 NA (NA)coding (411/1302 nt) PP_1865 transposase
* ? NC_002947 2103478 =18 (1.080)7 (0.430)
+TT
4/282 1.6 31.4% intergenic (+49/+27) msrC/PP_1878 free methionine‑(R)‑sulfoxide reductase/hypothetical protein
?NC_002947 4898402 = 13 (0.780)intergenic (+68/‑140) PP_4304/sbp‑I VIC family cation transporter/sulfate ABC transporter substrate‑binding protein
* ? NC_002947 = 226785415 (0.900)4 (0.240) 3/282 1.9 21.2% coding (949/1311 nt) folC bifunctional folypolyglutamate synthase/dihydrofolate synthase
?NC_002947 = 2267875 15 (0.910)coding (970/1311 nt) folC bifunctional folypolyglutamate synthase/dihydrofolate synthase
* ? NC_002947 = 233523614 (0.840)4 (0.240) 4/280 1.6 22.4% coding (203/1185 nt) fadA 3‑ketoacyl‑CoA thiolase
?NC_002947 = 2335264 14 (0.860)coding (231/1185 nt) fadA 3‑ketoacyl‑CoA thiolase
* ? NC_002947 2458799 =16 (0.960)4 (0.290) 4/234 1.3 29.5% intergenic (+85/‑96) gapB/PP_2150 glyceraldehyde‑3‑phosphate dehydrogenase/thiamine biosynthesis lipoprotein
?NC_002947 2458863 = 6 (0.440)intergenic (+149/‑32) gapB/PP_2150 glyceraldehyde‑3‑phosphate dehydrogenase/thiamine biosynthesis lipoprotein
* ? NC_002947 = 26854129 (0.540)3 (0.190) 3/272 1.8 24.4% coding (1118/1128 nt) PP_2352 hypothetical protein
?NC_002947 = 2685422 10 (0.630)coding (1108/1128 nt) PP_2352 hypothetical protein
* ? NC_002947 = 269383715 (0.900)4 (0.240) 3/286 1.9 25.8% coding (33/534 nt) PP_2359 putative Type 1 pili subunit CsuA/B protein
?NC_002947 = 2693873 8 (0.480)coding (69/534 nt) PP_2359 putative Type 1 pili subunit CsuA/B protein
* ? NC_002947 2703660 =9 (0.540)3 (0.190) 3/276 1.8 26.5% coding (136/1188 nt) PP_2367 transport protein
?NC_002947 2703708 = 8 (0.500)coding (88/1188 nt) PP_2367 transport protein
* ? NC_002947 = 279688711 (0.660)4 (0.260) 4/260 1.4 25.0% intergenic (+30/+82) PP_2447/puuB hypothetical protein/gamma‑glutamylputrescine oxidase
?NC_002947 = 2796938 14 (0.920)intergenic (+81/+31) PP_2447/puuB hypothetical protein/gamma‑glutamylputrescine oxidase
* ? NC_002947 = 297319312 (0.720)3 (0.190) 3/272 1.8 21.1% coding (1567/2391 nt) PP_2599 chemotaxis sensory transducer family protein
?NC_002947 = 2973206 11 (0.690)coding (1580/2391 nt) PP_2599 chemotaxis sensory transducer family protein
* ? NC_002947 3124809 =15 (0.900)5 (0.310) 3/274 1.8 25.4% coding (279/888 nt) PP_2740 MerR family transcriptional regulator
?NC_002947 3124830 = 15 (0.940)coding (300/888 nt) PP_2740 MerR family transcriptional regulator
* ? NC_002947 = 363785118 (1.080)6 (0.360) 4/286 1.6 27.3% coding (2284/2481 nt) PP_3202 transporter
?NC_002947 = 3637872 14 (0.840)coding (2305/2481 nt) PP_3202 transporter
* ? NC_002947 3773205 =5 (0.300)3 (0.190) 3/264 1.8 38.4% coding (653/1494 nt) PP_3331 membrane protein
?NC_002947 3773238 = 5 (0.320)coding (686/1494 nt) PP_3331 membrane protein
* ? NC_002947 3903718 =17 (1.020)5 (0.300) 3/286 1.9 22.7% coding (430/480 nt) PP_3444 glyoxalase family protein
?NC_002947 3903739 = 17 (1.020)coding (451/480 nt) PP_3444 glyoxalase family protein
* ? NC_002947 3949879 =7 (0.420)3 (0.220) 3/230 1.5 36.1% coding (136/1797 nt) PP_3483 type II secretion system protein
?NC_002947 3949964 = 5 (0.370)coding (51/1797 nt) PP_3483 type II secretion system protein
* ? NC_002947 = 39499068 (0.480)3 (0.220) 3/230 1.5 34.4% coding (109/1797 nt) PP_3483 type II secretion system protein
?NC_002947 = 3949935 5 (0.370)coding (80/1797 nt) PP_3483 type II secretion system protein
* ? NC_002947 3953036 =10 (0.600)3 (0.220) 3/230 1.5 31.5% intergenic (‑34/+161) PP_3486/PP_3487 cytochrome c/hypothetical protein
?NC_002947 3953152 = 5 (0.370)intergenic (‑150/+45) PP_3486/PP_3487 cytochrome c/hypothetical protein
* ? NC_002947 4132612 =20 (1.200)5 (0.300) 4/284 1.6 24.5% coding (245/687 nt) PP_3634 GNAT family acetyltransferase
?NC_002947 4132646 = 11 (0.660)coding (279/687 nt) PP_3634 GNAT family acetyltransferase
* ? NC_002947 4322733 =10 (0.600)4 (0.250) 4/270 1.5 29.1% intergenic (‑3/‑415) PP_3791/PP_5629 site‑specific recombinase/protease/amidase
?NC_002947 4322760 = 10 (0.640)intergenic (‑30/‑388) PP_3791/PP_5629 site‑specific recombinase/protease/amidase
* ? NC_002947 = 447796713 (0.780)4 (0.250) 4/272 1.5 24.4% coding (251/321 nt) PP_3966 transposase
?NC_002947 = 4477991 NA (NA)coding (227/321 nt) PP_3966 transposase
* ? NC_002947 = 45355585 (0.300)3 (0.200) 3/262 1.8 36.2% intergenic (+34/‑140) PP_4020/cpo oxidoreductase/non‑heme chloroperoxidase
?NC_002947 = 4535597 6 (0.390)intergenic (+73/‑101) PP_4020/cpo oxidoreductase/non‑heme chloroperoxidase
* ? NC_002947 = 45880226 (0.360)6 (0.490) 4/210 1.1 65.2% intergenic (+139/+78) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
?NC_002947 = 4588050 2 (0.160)intergenic (+167/+50) PP_4061/PP_4063 hypothetical protein/long‑chain fatty acid‑‑CoA ligase
* ? NC_002947 4649525 =12 (0.720)6 (0.490) 5/208 0.9 50.6% intergenic (+10/+121) fusB/PP_4112 elongation factor G 2/sulfate transporter
?NC_002947 4649633 = 3 (0.250)intergenic (+118/+13) fusB/PP_4112 elongation factor G 2/sulfate transporter
* ? NC_002947 4702712 =20 (1.200)5 (0.330) 4/258 1.4 25.0% intergenic (‑57/+93) kdpD/kdpC sensor protein/potassium‑transporting ATPase subunit C
?NC_002947 4702784 = 12 (0.800)intergenic (‑129/+21) kdpD/kdpC sensor protein/potassium‑transporting ATPase subunit C
* ? NC_002947 4725869 =8 (0.480)3 (0.190) 3/274 1.8 27.7% coding (215/483 nt) yigI hypothetical protein
?NC_002947 4725900 = 8 (0.500)coding (184/483 nt) yigI hypothetical protein
* ? NC_002947 = 48101466 (0.360)10 (0.660)
+CGGCGCTTCGCGG
7/260 0.8 50.0% intergenic (‑288/‑267) PP_4234/dsbD‑II bifunctional aldehyde oxidase/xanthine dehydrogenase/thiol/disulfide interchange protein 2
?NC_002947 4947183 = 16 (0.960)intergenic (+576/+222) PP_5673/flhB D‑alanine‑‑D‑alanine ligase/flagellin export apparatus substrate specificity protein
* ? NC_002947 5212189 =13 (0.780)5 (0.340) 3/254 1.7 30.7% intergenic (‑85/+93) rnd/PP_4592 ribonuclease D/metal dependent hydrolase
?NC_002947 5212280 = 11 (0.740)intergenic (‑176/+2) rnd/PP_4592 ribonuclease D/metal dependent hydrolase
* ? NC_002947 5335165 =4 (0.240)4 (0.270) 3/256 1.7 51.3% intergenic (+27/‑216) gluQ/PP_5704 glutamyl‑Q tRNA(Asp) synthetase/hypothetical protein
?NC_002947 5335225 = 4 (0.270)intergenic (+87/‑156) gluQ/PP_5704 glutamyl‑Q tRNA(Asp) synthetase/hypothetical protein
* ? NC_002947 = 55960926 (0.360)7 (0.520) 5/230 1.0 61.3% intergenic (‑66/+79) PP_4921/thiC NCS1 family transporter/phosphomethylpyrimidine synthase
?NC_002947 = 5596129 4 (0.300)intergenic (‑103/+42) PP_4921/thiC NCS1 family transporter/phosphomethylpyrimidine synthase
* ? NC_002947 5639467 =10 (0.600)3 (0.190) 3/274 1.8 21.8% coding (28/1443 nt) PP_4949 TldD/PmbA family peptidase
?NC_002947 5639485 = 12 (0.750)coding (46/1443 nt) PP_4949 TldD/PmbA family peptidase
* ? NC_002947 = 58060268 (0.480)3 (0.190) 3/276 1.8 26.4% coding (283/537 nt) pilN type IV pili biogenesis protein PilN
?NC_002947 = 5806044 9 (0.560)coding (265/537 nt) pilN type IV pili biogenesis protein PilN
* ? NC_002947 = 59057649 (0.540)4 (0.270) 4/254 1.4 36.4% intergenic (+42/+65) PP_5176/spuH hypothetical protein/spermidine/putrescine ABC transporter permease
?NC_002947 = 5905792 6 (0.410)intergenic (+70/+37) PP_5176/spuH hypothetical protein/spermidine/putrescine ABC transporter permease
* ? NC_002947 6029871 =9 (0.540)6 (0.420) 6/244 0.9 43.4% intergenic (‑37/+74) PP_5277/kauB MFS transporter/4‑guanidinobutyraldehyde dehydrogenase
?NC_002947 6029928 = 8 (0.560)intergenic (‑94/+17) PP_5277/kauB MFS transporter/4‑guanidinobutyraldehyde dehydrogenase
* ? NC_002947 = 60298919 (0.540)4 (0.280) 4/244 1.3 33.8% intergenic (‑57/+54) PP_5277/kauB MFS transporter/4‑guanidinobutyraldehyde dehydrogenase
?NC_002947 = 6029906 8 (0.560)intergenic (‑72/+39) PP_5277/kauB MFS transporter/4‑guanidinobutyraldehyde dehydrogenase
* ? NC_002947 6174998 =7 (0.420)3 (0.180) 3/284 1.9 27.3% coding (62/777 nt) PP_5410 DeoR family transcriptional regulator
?NC_002947 6175017 = 9 (0.540)coding (43/777 nt) PP_5410 DeoR family transcriptional regulator