breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 64,626 A→G G385G (GGT→GGC polB ← DNA polymerase II
RA 78,635 (G)5→6 coding (1015/1179 nt) setA → sugar exporter SetA
RA 92,022 C→T P204S (CCG→TCG)  ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 125,814 C→T D40D (GAC→GAT aceF → pyruvate dehydrogenase, E2 subunit
RA 202,830 C→T R91C (CGC→TGC)  lpxA → UDP‑N‑acetylglucosamine acyltransferase
RA 211,164 C→T P496S (CCG→TCG)  ldcC → lysine decarboxylase 2
RA 211,686 C→T R670C (CGC→TGC)  ldcC → lysine decarboxylase 2
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 282,806 C→T R177C (CGC→TGC)  yagE → CP4‑6 prophage; putative 2‑keto‑3‑deoxygluconate aldolase
RA 312,889 A→G intergenic (‑112/+252) ykgM ← / ← ykgR putative ribosomal protein/putative membrane protein YkgR
RA 316,465 T→C pseudogene (672/687 nt) ykgA ← putative DNA‑binding transcriptional regulator YkgA
RA 325,853 A→G R465R (CGT→CGC betA ← choline dehydrogenase
RA 343,869 A→G E329G (GAG→GGG)  yahK → aldehyde reductase, NADPH‑dependent
RA 350,125 T→C S38S (AGT→AGC prpC → 2‑methylcitrate synthase
RA 367,514 C→T intergenic (‑4/+73) lacI ← / ← mhpR DNA‑binding transcriptional repressor LacI/DNA‑binding transcriptional activator MhpR
RA 371,588 (G)6→7 coding (350/867 nt) mhpC → 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
RA 372,096 A→G A286A (GCA→GCG mhpC → 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
RA 399,283 A→G N17N (AAT→AAC yaiY ← inner membrane protein
RA 452,876 C→T A224T (GCC→ACC)  ampG ← muropeptide:H(+) symporter
RA 453,367 (A)5→6 coding (179/1476 nt) ampG ← muropeptide:H(+) symporter
RA 482,381 C→T G675S (GGT→AGT)  acrB ← multidrug efflux pump RND permease AcrB
RA 524,888 T→C L543S (TTG→TCG)  rhsD → protein RhsD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 549,208 T→C G284G (GGT→GGC ylbE → DUF1116 domain‑containing protein YlbE
RA 561,696 (G)6→7 coding (2000/2604 nt) sfmD → putative fimbrial usher protein SfmD
RA 626,394 C→T R109C (CGT→TGT)  entE → 2,3‑dihydroxybenzoate‑AMP ligase
RA 656,991 C→T I145I (ATC→ATT pagP → Lipid IVA palmitoyltransferase
RA 671,928 C→T A87T (GCA→ACA)  lptE ← lipopolysaccharide assembly protein LptE
RA 699,967 A→G R120R (CGT→CGC umpH ← UMP phosphatase
RA 732,974 G→A S1131N (AGC→AAC)  rhsC → rhs element protein RhsC
RA 752,962 (G)6→7 intergenic (‑167/+223) ybgD ← / ← gltA putative fimbrial protein YbgD/citrate synthase
RA 788,084 C→T A252T (GCC→ACC)  galM ← galactose‑1‑epimerase
RA 868,422 (C)8→7 coding (903/1872 nt) gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 894,989 C→T intergenic (+93/‑2) potF → / → potG putrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATP binding subunit
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 941,941 C→T P328L (CCA→CTA)  dmsA → dimethyl sulfoxide reductase subunit A
RA 956,966 +T coding (205/693 nt) ycaP → DUF421 domain‑containing protein YcaP
RA 980,658 A→G T1445A (ACC→GCC)  mukB → chromosome partitioning protein MukB
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,041,264 A→G L216L (TTA→TTG appA → periplasmic phosphoanhydride phosphatase/multiple inositol‑polyphosphate phosphatase
RA 1,053,516 (G)6→7 coding (2663/2745 nt) torS ← sensory histidine kinase TorS
RA 1,102,607 T→C K197R (AAG→AGG)  csgD ← DNA‑binding transcriptional dual regulator CsgD
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,210,758 C→T A138V (GCG→GTG)  mcrA → e14 prophage; 5‑methylcytosine‑specific restriction enzyme McrA
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,231,060 (A)6→7 coding (294/420 nt) umuD → DNA polymerase V protein UmuD
RA 1,238,009 T→C Y147H (TAT→CAT)  dadA → D‑amino acid dehydrogenase
RA 1,245,841 A→G Y512Y (TAT→TAC treA ← periplasmic trehalase
RA 1,298,691 A→G I32V (ATC→GTC)  ychE → putative inner membrane protein
RA 1,327,734 T→C intergenic (+7/+33) rluB → / ← btuR 23S rRNA pseudouridine(2605) synthase/cobinamide/cobalamin adenosyltransferase
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
RA 1,437,392 A→G R1131R (CGT→CGC ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,453,613 A→G G10G (GGT→GGC paaZ ← oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,464,087 T→C R183R (CGT→CGC paaX → DNA‑binding transcriptional repressor PaaX
RA 1,538,650 A→G R68R (CGT→CGC narY ← nitrate reductase Z subunit beta
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,547,687 C→G A96G (GCC→GGC)  fdnG → formate dehydrogenase N subunit alpha
RA 1,555,706 (G)6→7 intergenic (‑37/+120) maeA ← / ← sra malate dehydrogenase, NAD‑requiring/30S ribosomal subunit protein S22
RA 1,563,067 T→C T4A (ACC→GCC)  ddpX ← D‑alanyl‑D‑alanine dipeptidase
RA 1,590,521 A→G pseudogene (16/203 nt) yneL ← putative HTH‑type transcriptional regulator YneL
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,656,524 T→C V114A (GTT→GCT)  speG → spermidine N‑acetyltransferase
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,728,154 T→C G106G (GGT→GGC gloA → glyoxalase I
RA 1,738,112 A→G Y90C (TAC→TGC)  purR → DNA‑binding transcriptional repressor PurR
RA 1,741,941 G→A G177S (GGC→AGC)  cfa → cyclopropane fatty acyl phospholipid synthase
RA 1,763,242 A→G A256A (GCT→GCC sufB ← Fe‑S cluster scaffold complex subunit SufB
RA 1,807,950 T→C L52P (CTT→CCT)  yniA → putative kinase YniA
RA 1,847,724 (C)6→5 coding (285/1044 nt) selD ← selenide, water dikinase
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,875,056 (C)6→5 coding (521/822 nt) nimR ← DNA‑binding transcriptional repressor NimR
RA 1,877,189 T→C L94P (CTC→CCC)  yeaO → conserved protein YeaO
RA 1,879,362 (T)8→7 intergenic (‑107/+41) yeaQ ← / ← yoaG PF04226 family protein YeaQ/DUF1869 domain‑containing protein YoaG
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,882,687 G→A G259D (GGC→GAC)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,911,124 A→G A283A (GCA→GCG yebQ → putative transporter YebQ
RA 1,933,564 G→A F347F (TTC→TTT edd ← phosphogluconate dehydratase
RA 1,953,927 A→G E162E (GAA→GAG cmoB → tRNA U34 carboxymethyltransferase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,006,753 C→T L200L (CTG→TTG)  amyA → alpha‑amylase
RA 2,070,892 T→C pseudogene (107/426 nt) yeeP → CP4‑44 prophage; putative GTP‑binding protein
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,202,729 T→C G817G (GGT→GGC yehI → DUF4132 domain‑containing protein YehI
RA 2,263,065 T→C Q144R (CAG→CGG)  fruB ← fructose‑specific PTS multiphosphoryl transfer protein FruB
RA 2,329,648 T→C L50L (TTA→TTG yfaQ ← tandem DUF2300 domain‑containing protein YfaQ
RA 2,340,851 A→G S1107P (TCG→CCG)  yfaL ← putative autotransporter adhesin YfaL
RA 2,361,222 T→C T65A (ACG→GCG)  rhmD ← L‑rhamnonate dehydratase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,469,150 T→C V7A (GTA→GCA)  yfdI → CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase
RA 2,481,618 T→C T187A (ACA→GCA)  emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,510,259 G→A L210L (CTG→CTA yfeO → putative transport protein YfeO
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,529,127 G→A G350G (GGC→GGT ligA ← DNA ligase
RA 2,567,343 A→G G255G (GGT→GGC eutH ← putative inner membrane protein
RA 2,577,002 G→A T459I (ACT→ATT)  maeB ← malate dehydrogenase
RA 2,626,339 G→A V409M (GTG→ATG)  ppx → exopolyphosphatase
RA 2,631,526 A→G I337T (ATC→ACC)  guaA ← GMP synthetase
RA 2,658,432 G→A T168T (ACC→ACT hscA ← iron‑sulfur cluster biosynthesis chaperone HscA
RA 2,704,371 A→G T313T (ACT→ACC lepB ← signal peptidase I
RA 2,750,543 T→C T56A (ACC→GCC)  grpE ← nucleotide exchange factor GrpE
RA 2,766,869 C→T R318C (CGT→TGT)  rnlA → CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,825,612 T→C intergenic (‑36/‑220) mltB ← / → srlA membrane‑bound lytic murein transglycosylase B/sorbitol‑specific PTS enzyme IIC2 component
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,859,768 T→C S3S (TCT→TCC pphB → phosphoprotein phosphatase 2
RA 2,891,254 C→T S215S (TCG→TCA cysJ ← sulfite reductase, flavoprotein subunit
RA 2,927,899 T→C intergenic (+227/‑330) ppnN → / → sdaC nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC
RA 2,941,637 T→C intergenic (‑6/+13) ygdD ← / ← gcvA conserved inner membrane protein YgdD/DNA‑binding transcriptional dual regulator GcvA
RA 2,947,713 (T)7→8 intergenic (+30/+44) metV → / ← amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 3,026,030 G→A E89K (GAA→AAA)  guaD → guanine deaminase
RA 3,032,464 T→C G366G (GGT→GGC uacT → urate:H(+) symporter
RA 3,063,159 G→A G58D (GGC→GAC)  argK → methylmalonyl‑CoA mutase‑interacting GTPase YgfD
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,073,789 G→A intergenic (‑98/+187) epd ← / ← yggC D‑erythrose‑4‑phosphate dehydrogenase/P‑loop NTPase domain‑containing protein YggC
RA 3,101,039 (C)7→8 coding (585/720 nt) yggN ← putative EcfF
RA 3,178,288 T→C S58S (AGT→AGC tolC → outer membrane channel TolC
RA 3,179,134 T→C S340S (AGT→AGC tolC → outer membrane channel TolC
RA 3,193,489 T→C L209P (CTG→CCG)  yqiK → protein YqiK
RA 3,235,560 G→A Q106* (CAG→TAG)  rlmG ← 23S rRNA m(2)G1835 methyltransferase
RA 3,245,619 T→C R132R (CGT→CGC exuT → hexuronate transporter
RA 3,277,706 (A)7→6 coding (370/465 nt) yhaV → ribosome‑dependent mRNA interferase toxin YhaV
RA 3,281,723 T→C intergenic (+98/‑253) agaA → / → agaS putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase/putative galactosamine‑6‑phosphate deaminase/isomerase
RA 3,291,892 T→C D184D (GAT→GAC yraK → putative fimbrial adhesin YraK
RA 3,296,380 T→C F191S (TTC→TCC)  diaA → DnaA initiator‑associating factor for replication initiation
RA 3,339,332 T→C D245G (GAC→GGC)  mlaF ← intermembrane phospholipid transport system, ATP binding subunit MlaF
RA 3,339,365 G→A A234V (GCG→GTG)  mlaF ← intermembrane phospholipid transport system, ATP binding subunit MlaF
RA 3,350,184 (C)7→8 coding (276/654 nt) elbB ← low activity glyoxalase ElbB
RA 3,351,288 G→A P580S (CCA→TCA)  arcB ← sensory histidine kinase ArcB
RA 3,353,474 G→A Q193* (CAG→TAG)  yhcC ← radical SAM family oxidoreductase YhcC
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,355,029 T→C V102A (GTT→GCT)  gltB → glutamate synthase subunit GltB
RA 3,387,408 T→C T261A (ACG→GCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,394,828 G→A A477A (GCC→GCT yhdP ← AsmA2 domain‑containing protein
RA 3,404,716 G→A intergenic (+258/‑720) yhdH → / → accB acrylyl‑CoA reductase/biotin carboxyl carrier protein
RA 3,410,733 G→A E152K (GAA→AAA)  dusB → tRNA‑dihydrouridine synthase B
RA 3,421,762 (T)7→8 coding (438/1104 nt) yhdY → putative ABC transporter membrane subunit YhdY
RA 3,461,111 T→C V30A (GTT→GCT)  gspG → Type II secretion system protein GspG
RA 3,511,721 T→C E208E (GAA→GAG yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,573,145 G→A R120C (CGC→TGC)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,605,975 T→C R75R (CGT→CGC yhhN → conserved inner membrane enzyme YhhN
RA 3,639,218 G→A intergenic (+77/+167) pitA → / ← uspB metal phosphate:H(+) symporter PitA/putative universal stress (ethanol tolerance) protein B
RA 3,639,879 (T)8→7 intergenic (‑159/‑232) uspB ← / → uspA putative universal stress (ethanol tolerance) protein B/universal stress global stress response regulator
RA 3,728,118 (C)8→7 coding (1254/1455 nt) xylB ← xylulokinase
RA 3,743,852 T→C V37A (GTT→GCT)  yiaL → DUF386 domain‑containing protein YiaL
RA 3,756,719 G→A A370V (GCA→GTA)  yiaY ← L‑threonine dehydrogenase
RA 3,771,784 C→T intergenic (‑40/‑497) yibI ← / → mtlA DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
RA 3,802,488 T→C T191A (ACT→GCT)  waaO ← UDP‑D‑glucose:(glucosyl)LPS alpha‑1,3‑glucosyltransferase
RA 3,814,439 G→A intergenic (+52/‑55) dut → / → slmA deoxyuridine triphosphatase/nucleoid occlusion factor SlmA
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,894,996 C→T T115T (ACC→ACT yieF → chromate reductase
RA 3,900,259 T→C S107G (AGC→GGC)  yieL ← putative hydrolase YieL
RA 3,939,203 A→G E326G (GAA→GGA) 
V470V (GTT→GTC
rbsR →
hsrA ←
DNA‑binding transcriptional dual regulator RbsR
putative transporter HsrA
RA 3,970,324 A→G G64G (GGA→GGG wecB → UDP‑N‑acetylglucosamine 2‑epimerase
RA 3,997,902 A→G intergenic (+3/‑81) yigB → / → uvrD 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase/ssDNA translocase and dsDNA helicase ‑ DNA helicase II
RA 4,084,422 G→A Y467Y (TAC→TAT fdoG ← formate dehydrogenase O subunit alpha
RA 4,099,985 A→G C181R (TGC→CGC)  rhaT ← rhamnose/lyxose:H(+) symporter
RA 4,116,076 C→T E383K (GAG→AAG)  glpK ← glycerol kinase
RA 4,126,671 A→G V47A (GTA→GCA)  priA ← primosome factor N'
RA 4,148,286 C→T R11H (CGC→CAC)  yijO ← putative DNA‑binding transcriptional regulator YijO
RA 4,168,420 A→G noncoding (49/76 nt) gltT → tRNA‑Glu
RA 4,198,529 A→G T272A (ACA→GCA)  hemE → uroporphyrinogen decarboxylase
RA 4,246,520 C→T intergenic (‑101/‑264) malE ← / → malK maltose ABC transporter periplasmic binding protein/maltose ABC transporter ATP binding subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,361,448 (A)8→7 coding (487/1539 nt) cadC ← DNA‑binding transcriptional activator CadC
RA 4,366,861 T→C intergenic (‑88/+30) dcuA ← / ← aspA C4‑dicarboxylate transporter DcuA/aspartate ammonia‑lyase
RA 4,419,626 C→T intergenic (‑1/‑354) ulaG ← / → ulaA L‑ascorbate‑6‑phosphate lactonase/L‑ascorbate specific PTS enzyme IIC component
RA 4,435,918 A→G G216G (GGT→GGC cpdB ← 2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,455,998 A→C T72P (ACC→CCC)  mpl → UDP‑N‑acetylmuramate‑‑L‑alanyl‑gamma‑D‑glutamyl‑ meso‑2,6‑diaminoheptanedioate ligase
RA 4,490,370 A→G L257P (CTG→CCG)  idnR ← DNA‑binding transcriptional dual regulator IdnR
RA 4,569,605 A→G pseudogene (608/921 nt) rpnD → truncated recombination‑promoting nuclease‑like protein RpnD
RA 4,606,216 A→G noncoding (71/87 nt) leuP ← tRNA‑Leu
RA 4,628,442 C→T L376L (CTC→CTT nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
RA 4,639,714 T→C D198G (GAC→GGC)  arcA ← DNA‑binding transcriptional dual regulator ArcA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423766–3424234 3424525–3424239 6–760 20 [16] [15] 22 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =12 (0.170)68 (1.070) 44/242 0.2 85.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 12 (0.190)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780512 (0.170)42 (0.660) 37/242 0.5 78.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 12 (0.190)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)75 (1.080) 52/266 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =107 (1.490)93 (1.310) 60/272 0.1 63.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)92 (1.280) 52/274 0.2 97.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA