breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 5,144 G→A 83.6% intergenic (+124/‑90) thrC → / → yaaX L‑threonine synthase/DUF2502 family putative periplasmic protein
RA 5,789 A→C 9.9% L224R (CTG→CGG)  yaaA ← peroxide resistance protein, lowers intracellular iron
RA 10,444 G→T 89.5% F17L (TTC→TTA satP ← succinate‑acetate transporter
RA 12,901 G→A 100% D247N (GAT→AAT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ
RA 29,139 G→T 78.7% G256C (GGT→TGT)  dapB → dihydrodipicolinate reductase
RA 34,345 G→T 9.6% E16* (GAA→TAA)  caiF → cai operon transcriptional activator
RA 56,677 G→T 76.8% R145S (CGC→AGC)  lptD ← LPS assembly OM complex LptDE, beta‑barrel component
RA 65,335 G→T 5.7% P149Q (CCG→CAG)  polB ← DNA polymerase II
RA 68,504 T→G 9.7% L223V (TTG→GTG)  yabI → DedA family inner membrane protein
RA 72,337 C→A 27.8% D484Y (GAC→TAC)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 72,392 G→T 51.7% F465L (TTC→TTA sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 104,948 A→T 67.5% E61D (GAA→GAT secA → preprotein translocase subunit, ATPase
RA 105,563 C→A 8.7% D266E (GAC→GAA secA → preprotein translocase subunit, ATPase
RA 118,873 G→T 100% D99Y (GAC→TAC)  pdhR → pyruvate dehydrogenase complex repressor; autorepressor
RA 123,870 G→T 55.9% P563P (CCG→CCT aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 142,460 T→A 92.8% F298Y (TTC→TAC)  yadE → putative polysaccharide deacetylase lipoprotein
RA 147,946 G→T 47.6% I47I (ATC→ATA yadK ← putative fimbrial‑like adhesin protein
RA 153,472 G→T 74.9% intergenic (‑102/+268) yadN ← / ← folK putative fimbrial‑like adhesin protein/2‑amino‑4‑hydroxy‑6‑hydroxymethyldihyropteridine pyrophosphokinase
RA 157,178 C→A 52.8% intergenic (‑87/+91) dksA ← / ← sfsA transcriptional regulator of rRNA transcription, DnaK suppressor protein/sugar fermentation stimulation protein A
RA 158,287 C→A 83.5% D78Y (GAC→TAC)  ligT ← 2'‑5' RNA ligase
RA 161,171 G→T 8.7% intergenic (+150/‑46) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
RA 170,868 C→A 15.6% V168F (GTT→TTT)  hemL ← glutamate‑1‑semialdehyde aminotransferase (aminomutase)
RA 198,022 C→A 50.7% R189S (CGT→AGT)  lpxD → UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
RA 220,338 A→C 7.5% noncoding (81/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 230,579 C→T 100% E231K (GAA→AAA)  gloB ← hydroxyacylglutathione hydrolase
RA 232,860 G→T 90.4% D103Y (GAT→TAT)  dnaQ → DNA polymerase III epsilon subunit
RA 233,053 A→C 5.5% D167A (GAT→GCT)  dnaQ → DNA polymerase III epsilon subunit
RA 234,994 G→T 15.7% S77* (TCA→TAA)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 235,006 G→T 13.6% T73K (ACG→AAG)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 235,010 G→T 17.0% L72I (CTT→ATT)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 235,019 C→A 17.1% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 235,022 G→T 19.0% H68N (CAT→AAT)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 235,036 G→T 14.8% S63Y (TCC→TAC)  ykfM ← lethality reduction protein, putative inner membrane protein
RA 238,195 G→T 79.8% V532V (GTC→GTA fadE ← acyl coenzyme A dehydrogenase
RA 248,470 A→C 6.5% intergenic (+30/‑22) dinB → / → yafN DNA polymerase IV/antitoxin of the YafO‑YafN toxin‑antitoxin system
RA 249,629 G→A 8.3% R145Q (CGA→CAA)  yafP → putative acyl‑CoA transferase
RA 281,469 A→T 12.1% T122S (ACC→TCC)  yagG → CP4‑6 prophage; putative sugar transporter
RA 306,949 G→A 11.1% A33A (GCC→GCT ecpR ← putative transcriptional regulator for the ecp operon
RA 312,532 G→A 87.6% pseudogene (349/684 nt) ykgA ← pseudogene, AraC family;putative regulator; Not classified; putative ARAC‑type regulatory protein
RA 320,920 G→A 75.1% S52S (AGC→AGT ykgH ← putative inner membrane protein
RA 327,491 G→A 9.8% intergenic (+284/‑591) betT → / → yahA choline transporter of high affinity/c‑di‑GMP‑specific phosphodiesterase
RA 334,250 G→T 9.5% R72L (CGT→CTT)  yahG → DUF1116 family protein
RA 341,060 C→A 9.3% intergenic (+358/‑55) yahL → / → yahM uncharacterized protein/uncharacterized protein
RA 351,359 G→T 7.5% G243C (GGT→TGT)  codB → cytosine transporter
RA 353,486 A→T 6.0% intergenic (+321/+16) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon; autorepressor
RA 355,982 A→C 10.9% S94R (AGC→CGC)  cynX → putative cyanate transporter
RA 358,609 G→T 5.3% I94I (ATC→ATA lacY ← lactose permease
RA 360,173 C→A 7.6% intergenic (‑107/+488) lacZ ← / ← lacI pseudogene, truncated/DNA‑binding transcriptional repressor
RA 360,181 G→C 7.0% intergenic (‑115/+480) lacZ ← / ← lacI pseudogene, truncated/DNA‑binding transcriptional repressor
RA 360,752 A→G 100% V331A (GTG→GCG)  lacI ← DNA‑binding transcriptional repressor
JC 361,139 +GCCA 9.1% coding (605/1083 nt) lacI ← DNA‑binding transcriptional repressor
RA 361,806 G→A 100% intergenic (‑63/+14) lacI ← / ← mhpR DNA‑binding transcriptional repressor/mhp operon transcriptional activator
RA 362,159 G→T 73.7% I165I (ATC→ATA mhpR ← mhp operon transcriptional activator
RA 376,532 C→A 58.8% pseudogene (184/416 nt) yaiX ← pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
RA 379,943 C→T 5.2% G44R (GGA→AGA)  yaiS ← putative PIG‑L family deacetylase
RA 379,966 G→A 6.8% S36L (TCG→TTG)  yaiS ← putative PIG‑L family deacetylase
RA 380,508 C→T 6.1% intergenic (‑436/‑180) yaiS ← / → tauA putative PIG‑L family deacetylase/taurine transporter subunit
RA 389,725 G→T 8.9% pseudogene (1300/1449 nt) yaiT → pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
RA 402,340 G→T 90.5% V160V (GTG→GTT aroL → shikimate kinase II
RA 406,075 C→A 92.0% P159Q (CCG→CAG)  mak → manno(fructo)kinase
RA 411,027 G→T 86.3% L61L (CTC→CTA sbcC ← exonuclease, dsDNA, ATP‑dependent
RA 419,663 G→T 52.0% E564* (GAA→TAA)  malZ → maltodextrin glucosidase
RA 433,551 G→T 89.5% S14Y (TCC→TAC)  yajO ← 2‑carboxybenzaldehyde reductase
RA 434,663 A→C 87.8% I324S (ATC→AGC)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
RA 435,336 G→T 81.3% Q100K (CAG→AAG)  dxs ← 1‑deoxyxylulose‑5‑phosphate synthase, thiamine triphosphate‑binding, FAD‑requiring
RA 437,672 T→A 79.6% V223E (GTG→GAG)  thiI → tRNA s(4)U8 sulfurtransferase
RA 442,299 G→T 8.8% S288* (TCG→TAG)  cyoE ← protoheme IX farnesyltransferase
RA 456,588 G→T 90.5% D749Y (GAC→TAC)  lon → DNA‑binding ATP‑dependent protease La
RA 460,067 A→C 5.1% L70F (TTA→TTC fadM → long‑chain acyl‑CoA thioesterase III
RA 461,641 G→A 53.9% V376V (GTC→GTT ybaE ← putative ABC superfamily transporter periplasmic‑binding protein
RA 474,138 G→T 89.1% intergenic (‑65/+99) ylaB ← / ← ylaC putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase/DUF1449 family inner membrane protein
RA 479,244 C→A 67.4% A206S (GCC→TCC)  acrB ← multidrug efflux system protein
RA 480,820 C→T 91.0% D86N (GAC→AAC)  acrA ← multidrug efflux system
RA 486,021 A→C 8.2% L83R (CTG→CGG)  priC ← primosomal replication protein N''
RA 495,504 A→T 59.7% intergenic (‑75/‑77) aes ← / → gsk acetyl esterase/inosine/guanosine kinase
RA 530,466 C→A 90.9% F365L (TTC→TTA gcl → glyoxylate carboligase
RA 535,589 G→T 5.5% M329I (ATG→ATT allB → allantoinase
RA 542,200 Δ1 bp 73.4% coding (65/1668 nt) fdrA → putative NAD(P)‑binding acyl‑CoA synthetase
RA 546,491 G→T 87.0% V199V (GTG→GTT ybcF → putative carbonate kinase
RA 549,397 G→T 93.1% intergenic (‑1/+2) lpxH ← / ← ppiB UDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)
RA 553,502 C→A 12.6% intergenic (‑305/‑166) folD ← / → sfmA bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/ 5,10‑methylene‑tetrahydrofolate cyclohydrolase/FimA homolog
RA 578,719 G→T 11.5% intergenic (+128/‑418) tfaX → / → appY pseudogene, DLP12 prophage;Phage or Prophage Related/global transcriptional activator; DLP12 prophage
RA 579,710 G→A 59.4% A192T (GCA→ACA)  appY → global transcriptional activator; DLP12 prophage
RA 581,203 A→T 6.0% intergenic (‑114/‑310) ompT ← / → pauD DLP12 prophage; outer membrane protease VII (outer membrane protein 3b)/tRNA‑OTHER
RA 583,341 T→G 93.6% S33R (AGT→CGT)  ybcH ← PRK09936 family protein
RA 596,756 G→T 7.2% E863* (GAG→TAG)  cusA → copper/silver efflux system, membrane component
RA 601,154 T→A 8.2% L63F (TTA→TTT ybdF ← DUF419 family protein
RA 607,931 G→A 90.5% S7F (TCC→TTC)  fepA ← iron‑enterobactin outer membrane transporter
RA 608,174 G→A 7.4% intergenic (‑224/‑19) fepA ← / → fes iron‑enterobactin outer membrane transporter/enterobactin/ferric enterobactin esterase
RA 625,363 G→T 6.0% G5V (GGG→GTG) ‡ cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
RA 625,364 G→T 60.1% G5G (GGG→GGT) ‡ cstA → carbon starvation protein involved in peptide utilization; APC peptide transporter family protein
RA 628,153 T→A 10.8% N261Y (AAC→TAC)  ybdH ← putative oxidoreductase
RA 632,406 T→A 52.0% K223N (AAA→AAT ybdO ← putative DNA‑binding transcriptional regulator
RA 639,231 G→A 90.6% L65F (CTT→TTT)  rnk ← regulator of nucleoside diphosphate kinase
RA 641,886 C→A 55.4% E51* (GAG→TAG)  citT ← citrate:succinate antiporter
RA 647,422 G→T 76.0% intergenic (‑110/‑269) citC ← / → citA [citrate [pro‑3S]‑lyase] ligase/sensory histidine kinase in two‑component regulatory system with CitB
RA 648,056 C→A 90.2% F122L (TTC→TTA citA → sensory histidine kinase in two‑component regulatory system with CitB
RA 652,865 Δ1 bp 84.7% coding (118/210 nt) cspE → constitutive cold shock family transcription antitermination protein; negative regulator of cspA transcription; RNA melting protein; ssDNA‑binding protein
RA 660,816 G→A 7.9% V318V (GTC→GTT mrdB ← cell wall shape‑determining protein
RA 662,631 C→A 78.5% R348L (CGC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 668,211 C→A 5.6% V677F (GTT→TTT)  leuS ← leucyl‑tRNA synthetase
RA 681,491 A→C 8.2% F212C (TTT→TGT)  gltJ ← glutamate, aspartate ABC transporter permease subunit
RA 705,588 C→A 77.1% intergenic (+16/+68) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 707,409 C→A 8.1% intergenic (‑55/+85) ybfE ← / ← ybfF LexA‑regulated protein, CopB family/acyl‑CoA esterase
RA 754,572 G→T 94.6% E137D (GAG→GAT sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 757,524 C→A 7.2% R183S (CGC→AGC)  sucB → dihydrolipoyltranssuccinase
RA 759,000 C→A 94.7% F177L (TTC→TTA sucC → succinyl‑CoA synthetase, beta subunit
RA 760,181 G→T 8.2% P182P (CCG→CCT sucD → succinyl‑CoA synthetase, NAD(P)‑binding, alpha subunit
RA 763,080 C→T 5.0% T547T (ACC→ACT mngA → fused 2‑O‑a‑mannosyl‑D‑glycerate specific PTS enzymes: IIA component/IIB component/IIC component
RA 773,443 C→A 10.6% A83E (GCG→GAG)  tolB → periplasmic protein
RA 788,902 G→T 89.5% R115S (CGT→AGT)  modF ← fused molybdate transporter subunits of ABC superfamily: ATP‑binding components
RA 800,779 G→T 92.1% E607D (GAG→GAT ybhJ → putative hydratase
RA 828,374 G→T 88.3% V11V (GTC→GTA ybiA ← DUF1768 family protein
RA 832,574 G→T 5.5% T256T (ACG→ACT ybiC → putative dehydrogenase
RA 868,618 G→T 9.1% E62* (GAA→TAA)  yliE → putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 871,072 C→A 12.4% V94V (GTC→GTA yliF → putative membrane‑anchored diguanylate cyclase
RA 874,768 G→T 9.6% E191* (GAA→TAA)  yliI → soluble aldose sugar dehydrogenase
RA 876,447 G→A 88.9% D89N (GAT→AAT)  dacC → D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 6a)
RA 879,085 G→T 86.5% intergenic (‑241/‑44) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein
RA 882,441 A→T 7.3% S119T (TCT→ACT)  ybjJ ← putative transporter
RA 920,469 G→T 5.5% G584C (GGT→TGT)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 923,312 G→T 9.3% S447* (TCG→TAG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 923,339 G→T 29.0% S438* (TCG→TAG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 924,296 A→C 7.2% L119W (TTG→TGG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 924,344 A→T 13.2% L103* (TTG→TAG)  cydC ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 947,211 C→A 5.3% P600P (CCG→CCT pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 948,890 C→A 8.3% E41* (GAG→TAG)  pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 950,103 T→C 30.8% intergenic (‑181/+225) focA ← / ← ycaO formate channel/ribosomal protein S12 methylthiotransferase accessory factor
RA 968,415 G→T 89.4% S148* (TCG→TAG)  ycbC ← DUF218 superfamily protein
RA 979,650 T→A 5.8% L182Q (CTG→CAG)  ycbL → putative metal‑binding enzyme
RA 1,006,218 G→T 9.4% E267* (GAG→TAG)  uup → putative transporter subunit of ABC superfamily: ATP‑binding component
RA 1,011,972 A→T 7.0% intergenic (‑46/+23) fabA ← / ← ycbZ beta‑hydroxydecanoyl thioester dehydrase/putative peptidase
RA 1,050,246 G→T 9.2% L463L (CTC→CTA torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,077,911 A→C 89.9% K71N (AAA→AAC efeO → inactive ferrous ion transporter EfeUOB
RA 1,090,918 T→A 7.7% intergenic (‑25/‑43) insE1 ← / → ymdE IS3 transposase A/pseudogene
RA 1,116,964 A→G 88.5% intergenic (‑21/+53) dinI ← / ← pyrC DNA damage‑inducible protein I/dihydro‑orotase
RA 1,118,740 G→T 7.0% intergenic (‑11/+123) yceB ← / ← grxB lipoprotein, DUF1439 family/glutaredoxin 2 (Grx2)
RA 1,129,363 G→A 13.1% A36T (GCG→ACG)  flgF → flagellar component of cell‑proximal portion of basal‑body rod
RA 1,136,758 T→A 10.3% A1022A (GCA→GCT rne ← fused ribonucleaseE: endoribonuclease/RNA‑binding protein/RNA degradosome binding protein
RA 1,151,750 G→T 74.9% R178L (CGC→CTC)  holB → DNA polymerase III, delta prime subunit
RA 1,152,278 G→T 89.1% E16* (GAA→TAA)  ycfH → putative DNase
RA 1,193,873 G→T 88.5% intergenic (‑180/‑278) ymfE ← / → lit e14 prophage; putative inner membrane protein/e14 prophage; cell death peptidase, inhibitor of T4 late gene expression
RA 1,194,365 G→T 6.4% W72L (TGG→TTG)  lit → e14 prophage; cell death peptidase, inhibitor of T4 late gene expression
RA 1,197,027 G→T 5.9% M25I (ATG→ATT ymfI → e14 prophage; uncharacterized protein
RA 1,215,174 G→T 51.3% pseudogene (118/2648 nt) ycgH → pseudogene; putative ATP‑binding component of a transport system
RA 1,217,884 A→T 9.5% I69I (ATT→ATA ymgD ← uncharacterized protein
RA 1,220,996 G→T 86.6% R181R (CGG→AGG)  minC ← cell division inhibitor
RA 1,228,591 G→T 89.2% intergenic (‑105/+41) dsbB ← / ← nhaB oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I/sodium:proton antiporter
RA 1,229,460 G→T 6.1% F238L (TTC→TTA nhaB ← sodium:proton antiporter
RA 1,243,278 G→A 7.1% S431S (AGC→AGT dhaM ← putative dihydroxyacetone‑specific PTS enzymes: HPr component/EI component
RA 1,245,461 C→A 8.2% V278V (GTG→GTT dhaK ← dihydroxyacetone kinase, PTS‑dependent, dihydroxyacetone‑binding subunit
RA 1,247,376 G→T 10.8% V285V (GTG→GTT dhaR → dhaKLM operon transcription activator
RA 1,282,421 A→G 9.5% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,306,244 G→A 6.8% S87L (TCG→TTG)  yciA ← acyl‑CoA esterase
RA 1,318,864 G→T 8.3% L170F (TTG→TTT yciO → putative RNA binding protein
RA 1,322,140 T→A 87.4% T159S (ACG→TCG)  btuR ← cob(I)yrinic acid a,c‑diamide adenosyltransferase
RA 1,345,484 A→C 5.8% intergenic (‑188/+180) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
RA 1,365,202 C→A 7.2% H244N (CAT→AAT)  ycjM → alpha amylase catalytic domain family protein
RA 1,365,283 G→T 78.3% E271* (GAG→TAG)  ycjM → alpha amylase catalytic domain family protein
JC JC 1,376,043 IS5 (–) +4 bp 100% pseudogene (10‑13/126 nt) ycjV → pseudogene; putative ATP‑binding component of a transport system
RA 1,377,394 C→T 84.9% A275A (GCG→GCA ycjW ← LacI family putative transcriptional repressor
RA 1,380,297 C→A 6.5% S177* (TCA→TAA)  ycjF → UPF0283 family inner membrane protein
RA 1,386,008 G→T 80.5% Q35K (CAG→AAG)  ycjY ← S9 homolog non‑peptidase family protein
RA 1,388,406 G→T 73.4% R308L (CGC→CTC)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
RA 1,398,482 C→A 5.6% R114L (CGT→CTT)  abgA ← p‑aminobenzoyl‑glutamate hydrolase, A subunit
RA 1,460,752 C→T 5.2% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,761 A→G 6.2% pseudogene (1113/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,471,707 A→T 53.9% *769C (TGA→TGT ydbD → PF10971 family putative periplasmic methylglyoxal resistance protein
RA 1,502,324 C→A 9.6% T429T (ACC→ACA ydcP → putative peptidase
RA 1,503,048 G→T 5.4% intergenic (+49/+43) ydcP → / ← yncJ putative peptidase/uncharacterized protein
RA 1,513,778 G→T 11.1% T165T (ACG→ACT curA → curcumin/dihydrocurcumin reductase, NADPH‑dependent
RA 1,519,349 T→A 90.0% K297* (AAA→TAA)  ansP ← L‑asparagine transporter
RA 1,524,654 G→T 6.8% W159L (TGG→TTG)  ydcD → uncharacterized protein
RA 1,538,906 G→A 8.2% pseudogene (116/381 nt) yddK ← pseudogene, leucine‑rich protein; putative glycoportein
RA 1,539,484 G→A 31.4% pseudogene (488/951 nt) yddK ← pseudogene, leucine‑rich protein; putative glycoportein
RA 1,548,144 G→T 89.0% intergenic (‑49/+85) adhP ← / ← maeA ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, (decarboxylating, NAD‑requiring) (malic enzyme)
RA 1,563,276 G→T 93.5% H491N (CAC→AAC)  gadC ← glutamate:gamma‑aminobutyric acid antiporter
RA 1,565,497 G→A 91.6% S269L (TCG→TTG)  gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,566,172 T→A 7.6% Q44L (CAG→CTG)  gadB ← glutamate decarboxylase B, PLP‑dependent
RA 1,571,071 T→A 10.8% N269I (AAC→ATC)  yddB ← putative TonB‑dependent outer membrane receptor
RA 1,576,869 G→A 6.0% intergenic (‑88/+314) ydeN ← / ← ydeO putative Ser‑type periplasmic non‑aryl sulfatase/UV‑inducible global regulator, EvgA‑, GadE‑dependent
RA 1,579,818 A→T 5.7% L309* (TTG→TAG)  ydeP ← putative oxidoreductase
RA 1,604,068 G→T 43.5% T290T (ACC→ACA uxaB ← altronate oxidoreductase, NAD‑dependent
RA 1,628,207 G→A 100% G110E (GGA→GAA)  pinQ → Qin prophage; putative site‑specific recombinase
RA 1,640,268 A→C 6.2% K39Q (AAG→CAG)  ydfV → Qin prophage; uncharacterized protein
RA 1,649,456 C→T 53.4% intergenic (‑58/+148) rspA ← / ← ynfA bifunctional D‑altronate/D‑mannonate dehydratase/UPF0060 family inner membrane protein
RA 1,661,763 C→A 87.1% P353P (CCG→CCT mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor
RA 1,662,484 G→A 5.8% S113L (TCG→TTG)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor
RA 1,671,110 C→A 7.2% D351Y (GAT→TAT)  pntA ← pyridine nucleotide transhydrogenase, alpha subunit
RA 1,672,822 A→T 12.4% N47Y (AAT→TAT)  ydgH → DUF1471 family periplasmic protein
RA 1,673,380 T→A 7.7% S233T (TCA→ACA)  ydgH → DUF1471 family periplasmic protein
RA 1,701,282 A→C 6.2% Q36P (CAG→CCG)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,101 C→G 8.6% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,703,104 G→A 5.3% E643E (GAG→GAA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,711,397 C→T 90.4% R28Q (CGA→CAA)  tyrS ← tyrosyl‑tRNA synthetase
RA 1,748,094 T→A 9.6% intergenic (‑7/+14) ydhV ← / ← ydhY putative oxidoreductase subunit/putative 4Fe‑4S ferridoxin‑type protein; FNR, Nar, NarP‑regulated protein; putative subunit of YdhYVWXUT oxidoreductase complex
RA 1,770,281 T→A 76.4% D146E (GAT→GAA ydiF → putative acetyl‑CoA:acetoacetyl‑CoA transferase: alpha subunit/beta subunit
RA 1,784,952 A→G 53.7% F184L (TTT→CTT)  ydiU ← UPF0061 family protein
RA 1,802,047 G→A 84.3% intergenic (+100/‑6) ydiZ → / → yniA uncharacterized protein/fructosamine kinase family protein
RA 1,807,639 G→A 6.6% intergenic (‑250/+39) ydjO ← / ← cedA uncharacterized protein/cell division modulator
RA 1,823,835 A→C 6.2% W53G (TGG→GGG)  astD ← succinylglutamic semialdehyde dehydrogenase
RA 1,832,413 C→A 75.9% L315L (CTC→CTA ynjC → inner membrane putative ABC superfamily transporter permease
RA 1,833,730 C→A 92.8% R3S (CGT→AGT)  ynjE → molybdopterin synthase sulfurtransferase
RA 1,849,412 T→A 9.3% T273S (ACT→TCT)  ydjG ← alpha‑Keto reductase, NADH‑dependent; can use methylglyoxal as substrate
RA 1,859,012 C→G 7.3% intergenic (+21/+27) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,865,422 C→A 92.5% R261R (CGA→AGA)  yeaI → putative membrane‑anchored diguanylate cyclase
RA 1,872,520 G→T 88.3% P183P (CCG→CCT yeaP → diguanylate cyclase
RA 1,909,342 T→A 89.5% E150D (GAA→GAT proQ ← RNA chaperone, putative ProP translation regulator
RA 1,915,555 A→C 14.4% Q359P (CAG→CCG)  rsmF → 16S rRNA m(5)C1407 methyltransferase, SAM‑dependent
RA 1,924,898 T→A 13.9% D36V (GAT→GTT)  yebG ← DNA damage‑inducible protein regulated by LexA
RA 1,934,928 G→T 7.6% P322Q (CCG→CAG)  mepM ← murein DD‑endopeptidase, space‑maker hydrolase, septation protein
RA 1,944,177 C→A 77.8% L201L (CTG→CTT aspS ← aspartyl‑tRNA synthetase
RA 1,995,152 T→A 12.4% E40V (GAA→GTA)  fliA ← RNA polymerase, sigma 28 (sigma F) factor
RA 2,020,166 C→A 75.5% D445Y (GAC→TAC)  yedQ ← putative membrane‑anchored diguanylate cyclase
RA 2,070,058 C→A 55.0% A90A (GCC→GCA yeeS → CP4‑44 prophage; putative DNA repair protein
RA 2,078,521 C→A 51.8% V162V (GTG→GTT yeeE ← UPF0394 family inner membrane protein
RA 2,098,995 C→A 81.5% G182* (GGA→TGA)  wbbI ← d‑Galf:alpha‑d‑Glc beta‑1,6‑galactofuranosyltransferase
RA 2,114,911 A→T 74.9% L42Q (CTG→CAG)  wcaJ ← colanic biosynthesis UDP‑glucose lipid carrier transferase
RA 2,127,716 G→T 15.7% S473* (TCG→TAG)  wzc ← colanic acid production tyrosine‑protein kinase; autokinase; Ugd phosphorylase
RA 2,129,796 C→A 5.3% R310L (CGT→CTT)  wza ← colanic acid export protein; outer membrane auxillary lipoprotein
RA 2,136,421 A→G 24.6% D3D (GAT→GAC udk ← uridine/cytidine kinase
RA 2,139,910 G→T 92.1% R1055L (CGA→CTA)  yegE → putative diguanylate cyclase
RA 2,144,474 C→A 90.2% intergenic (‑8/‑192) yegI ← / → yegJ protein kinase‑related putative non‑specific DNA‑binding protein/uncharacterized protein
RA 2,170,708 G→T 6.2% intergenic (‑25/+283) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,713 C→A 20.1% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,724 G→T 15.8% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,170,757 T→A 8.8% intergenic (‑74/+234) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
RA 2,195,449 A→T 91.6% E564D (GAA→GAT yehI → DUF4132 domain‑containing protein
RA 2,216,296 C→A 75.4% I211I (ATC→ATA dld → D‑lactate dehydrogenase, FAD‑binding, NADH independent
RA 2,237,098 G→T 79.5% H12N (CAT→AAT)  folE ← GTP cyclohydrolase I
RA 2,237,441 G→T 14.6% R18L (CGA→CTA)  yeiG → S‑formylglutathione hydrolase
RA 2,242,501 C→A 8.0% E199D (GAG→GAT yeiE ← putative DNA‑binding transcriptional regulator
RA 2,254,517 C→T 57.9% A125T (GCC→ACC)  fruA ← fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
RA 2,258,182 A→T 5.6% K281* (AAG→TAG)  setB → lactose/glucose efflux system
RA 2,264,143 G→A 78.2% intergenic (+119/‑62) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 2,289,374 C→A 90.0% V142V (GTG→GTT ccmC ← heme exporter subunit
RA 2,295,611 C→T 7.7% A208T (GCG→ACG)  napA ← nitrate reductase, periplasmic, large subunit
RA 2,298,128 C→T 5.8% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,159 T→C 5.9% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,167 A→G 7.1% intergenic (+295/+420) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,298,492 A→G 5.3% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,116 C→A 89.5% intergenic (‑103/+9) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
RA 2,319,735 G→T 5.4% E50* (GAA→TAA)  atoB → acetyl‑CoA acetyltransferase
RA 2,352,573 C→A 88.9% P353P (CCG→CCT rhmT ← putative L‑rhamnonate transporter
RA 2,359,673 G→T 89.9% M89I (ATG→ATT arnB → uridine 5'‑(beta‑1‑threo‑pentapyranosyl‑4‑ulose diphosphate) aminotransferase, PLP‑dependent
RA 2,362,338 T→A 84.7% L274Q (CTG→CAG)  arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
RA 2,370,361:1 +G 89.2% coding (711/759 nt) menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,370,987 C→A 90.0% E29* (GAA→TAA)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,376,381 G→A 14.4% A64T (GCT→ACT)  elaD → protease, capable of cleaving an AMC‑ubiquitin model substrate
RA 2,390,862 C→A 10.8% V20V (GTG→GTT nuoH ← NADH:ubiquinone oxidoreductase, membrane subunit H
RA 2,399,210 G→T 5.8% S304* (TCA→TAA)  lrhA ← transcriptional repressor of flagellar, motility and chemotaxis genes
RA 2,405,946 T→G 91.2% Q43P (CAG→CCG)  yfbU ← UPF0304 family protein
RA 2,407,495 G→T 11.6% G183C (GGC→TGC)  ackA → acetate kinase A and propionate kinase 2
RA 2,407,538 T→G 8.3% L197R (CTG→CGG)  ackA → acetate kinase A and propionate kinase 2
RA 2,422,841 C→A 87.7% D293Y (GAT→TAT)  purF ← amidophosphoribosyltransferase
RA 2,424,074 C→T 87.2% D57N (GAC→AAC)  cvpA ← colicin V production membrane protein
RA 2,447,733 A→C 7.8% pseudogene (748/2646 nt) yfcU ← pseudogene, FimD fimbrial export usher family;putative membrane; Not classified; putative outer membrane protein
RA 2,450,335 C→T 90.7% intergenic (‑44/+159) sixA ← / ← fadJ phosphohistidine phosphatase/fused enoyl‑CoA hydratase and epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
RA 2,454,297 T→G 78.0% E39D (GAA→GAC yfcZ ← UPF0381 family protein
RA 2,463,906 C→A 14.2% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,463,915 C→A 10.8% Q436K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,473,273 A→T 5.9% K198I (AAA→ATA)  dsdA → D‑serine dehydratase
RA 2,474,418 G→T 85.7% S413* (TCA→TAA)  emrY ← putative multidrug efflux system
RA 2,493,283 C→A 22.5% N378K (AAC→AAA ypdA → sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system
RA 2,520,863 G→T 6.8% D187Y (GAC→TAC)  yfeH → putative inorganic ion transporter
RA 2,553,563 T→G 9.8% intergenic (+138/‑53) intZ → / → yffL CPZ‑55 prophage; putative phage integrase/CPZ‑55 prophage; uncharacterized protein
RA 2,576,325 C→A 90.1% E171D (GAG→GAT nudK ← GDP‑mannose pyrophosphatase
RA 2,587,029:1 +G 87.6% coding (101/699 nt) ypfH ← palmitoyl‑CoA esterase activity, uncertain physiological substrate
RA 2,605,773 A→T 8.2% D172V (GAT→GTT)  hyfR → hydrogenase‑4 transcriptional activator
RA 2,605,882 C→A 11.9% S208R (AGC→AGA hyfR → hydrogenase‑4 transcriptional activator
RA 2,606,516 G→T 87.3% D420Y (GAC→TAC)  hyfR → hydrogenase‑4 transcriptional activator
RA 2,609,187 C→A 57.7% A18A (GCG→GCT yfgO ← putative UPF0118 family inner membrane permease
RA 2,614,168 C→A 88.6% D22Y (GAT→TAT)  upp ← uracil phosphoribosyltransferase
RA 2,651,665 G→T 88.7% G210G (GGC→GGA hscA ← DnaK‑like molecular chaperone specific for IscU
RA 2,653,668 G→T 9.0% intergenic (‑20/+8) iscU ← / ← iscS iron‑sulfur cluster assembly scaffold protein/cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
RA 2,654,099 C→A 7.7% A264A (GCG→GCT iscS ← cysteine desulfurase (tRNA sulfurtransferase), PLP‑dependent
RA 2,655,839 A→T 8.0% intergenic (‑349/+103) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
RA 2,671,274 C→A 92.1% T149T (ACG→ACT yphE ← putative sugar transporter subunit of ABC superfamily, ATP‑binding component
RA 2,674,941 G→T 46.1% I388I (ATC→ATA yphG ← DUF4380 domain‑containing TPR repeat protein
RA 2,679,933 C→A 7.9% A247D (GCT→GAT)  hmp → fused nitric oxide dioxygenase/dihydropteridine reductase 2
RA 2,681,873 T→A 88.1% Q97L (CAG→CTG)  glrR ← response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK
RA 2,702,974 C→A 6.2% R133L (CGA→CTA)  rpoE ← RNA polymerase sigma E factor
RA 2,708,587 C→A 7.7% T31T (ACG→ACT yfiE ← putative DNA‑binding transcriptional regulator
RA 2,715,643 G→T 91.5% E778* (GAA→TAA)  pka → protein lysine acetyltransferase
RA 2,718,953 G→T 52.2% I51I (ATC→ATA kgtP ← alpha‑ketoglutarate transporter
RA 2,719,426 T→C 100% intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,733,753 C→T 100% E253K (GAG→AAG)  aroF ← 3‑deoxy‑D‑arabino‑heptulosonate‑7‑phosphate synthase, tyrosine‑repressible
RA 2,759,184 C→T 6.0% intergenic (‑49/‑93) yfjM ← / → rnlA CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; RNase LS
RA 2,767,403 G→A 6.3% D243N (GAT→AAT)  yfjW → CP4‑57 prophage; putative inner membrane protein
RA 2,771,358 G→A 77.8% intergenic (+217/+147) ypjF → / ← ypjA CP4‑57 prophage; toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter
RA 2,775,830 C→T 52.0% D86N (GAT→AAT)  ypjA ← adhesin‑like autotransporter
RA 2,777,166 A→T 7.4% pseudogene (1205/1374 nt) ypjC ← pseudogene
RA 2,804,103 G→A 7.9% intergenic (+65/‑26) ygaH → / → mprA putative L‑valine exporter, norvaline resistance protein/transcriptional repressor of microcin B17 synthesis and multidrug efflux
RA 2,825,783 C→T 87.5% intergenic (‑135/‑52) norR ← / → norV anaerobic nitric oxide reductase DNA‑binding transcriptional activator/anaerobic nitric oxide reductase flavorubredoxin
RA 2,834,252 A→T 5.0% E457V (GAG→GTG)  ascF → fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
RA 2,835,291 T→G 7.9% C315G (TGC→GGC)  ascB → cryptic 6‑phospho‑beta‑glucosidase
RA 2,835,413 C→A 7.2% I355I (ATC→ATA ascB → cryptic 6‑phospho‑beta‑glucosidase
RA 2,850,790 C→A 86.3% I113I (ATC→ATA mutS → methyl‑directed mismatch repair protein
RA 2,873,087 G→T 89.3% V15V (GTC→GTA ygbT ← multifunctional endonuclease Cas1, CRISPR adaptation protein; DNA repair enzyme
RA 2,876,760 C→T 89.3% G246G (GGG→GGA casA ← CRISP RNA (crRNA) containing Cascade antiviral complex protein
RA 2,878,668 G→T 75.0% V637V (GTC→GTA ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
RA 2,890,873 A→T 12.1% I119I (ATT→ATA ygcS ← putative transporter
RA 2,899,859 G→T 6.2% G264C (GGT→TGT)  ygcG → uncharacterized protein
RA 2,928,446 G→T 51.7% A285S (GCT→TCT)  fucP → L‑fucose transporter
RA 2,931,717 C→A 88.4% N307K (AAC→AAA fucK → L‑fuculokinase
RA 2,934,843 G→T 87.6% H50N (CAT→AAT)  ygdD ← UPF0382 family inner membrane protein
RA 2,936,672 C→A 15.4% intergenic (‑168/‑24) ygdI ← / → csdA DUF903 family verified lipoprotein/cysteine sulfinate desulfinase
RA 2,983,868 G→T 55.6% intergenic (‑149/‑45) yqeK ← / → ygeF uncharacterized protein/pseudogene
RA 2,988,774 C→A 27.7% pseudogene (346/447 nt) ygeN ← pseudogene
RA 2,989,295 C→A 93.1% pseudogene (85/261 nt) ygeN ← pseudogene
RA 2,993,211 C→A 91.7% V14L (GTG→TTG)  ygeR ← LysM domain‑containing M23 family putative peptidase; septation lipoprotein
RA 3,001,919 C→A 26.1% H351N (CAT→AAT)  ygeX → 2,3‑diaminopropionate ammonia lyase, PLP‑dependent
RA 3,010,725 A→G 14.1% K436R (AAA→AGA)  ygfK → putative Fe‑S subunit oxidoreductase subunit
RA 3,013,226 C→A 5.2% S236Y (TCC→TAC)  ssnA → putative chlorohydrolase/aminohydrolase
RA 3,020,629 C→A 50.3% I50I (ATC→ATA ygfQ → putative purine permease
RA 3,024,983 C→G 77.3% G86G (GGC→GGG uacT → uric acid permease
RA 3,039,409 T→G 8.4% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase with NAD(P)‑binding Rossmann‑fold domain/glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
RA 3,044,464 G→A 90.0% intergenic (‑438/+10) gcvT ← / ← ubiI aminomethyltransferase, tetrahydrofolate‑dependent, subunit (T protein) of glycine cleavage complex/2‑octaprenylphenol hydroxylase, FAD‑dependent
RA 3,050,166 C→A 8.7% intergenic (+18/‑42) fau → / → sibC 5‑formyltetrahydrofolate cyclo‑ligase family protein/sRNA antisense regulator of toxic IbsC protein
RA 3,050,591 C→A 26.6% A393A (GCG→GCT serA ← D‑3‑phosphoglycerate dehydrogenase
RA 3,075,114 C→A 91.2% intergenic (‑120/‑158) tktA ← / → loiP transketolase 1, thiamine triphosphate‑binding/Phe‑Phe periplasmic metalloprotease, OM lipoprotein; low salt‑inducible; heat shock protein that binds Era
RA 3,076,526 C→A 78.8% D211Y (GAC→TAC)  speB ← agmatinase
RA 3,080,618 T→A 94.4% V185E (GTG→GAG)  metK → S‑adenosylmethionine synthetase
RA 3,080,755 T→G 20.3% F231V (TTC→GTC)  metK → S‑adenosylmethionine synthetase
RA 3,080,759 T→G 8.9% V232G (GTT→GGT)  metK → S‑adenosylmethionine synthetase
RA 3,103,319 C→T 5.3% I136I (ATC→ATT yqgA → DUF554 family putative inner membrane protein
RA 3,105,207 G→T 15.7% pseudogene (243/963 nt) yghE ← pseudogene, secretion pathway protein, L‑type protein homology; putative general secretion pathway for protein export (GSP)
RA 3,111,434 C→A 90.9% A346A (GCG→GCT yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,120,216 C→A 91.6% D389Y (GAT→TAT)  glcD ← glycolate oxidase subunit, FAD‑linked
RA 3,173,669 G→T 77.0% A189A (GCG→GCT ygiB → DUF1190 family protein
RA 3,182,319 G→T 9.4% pseudogene (1540/2445 nt) yqiG → pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein
RA 3,185,542 C→T 5.5% intergenic (‑244/‑25) glgS ← / → yqiJ motility and biofilm regulator/DUF1449 family inner membrane protein
RA 3,194,733 G→T 10.6% V56V (GTG→GTT ygiM → SH3 domain protein
RA 3,202,506 C→A 5.7% S375* (TCG→TAG)  ttdT → L‑tartrate/succinate antiporte
RA 3,211,932 C→A 7.8% V168V (GTG→GTT aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
RA 3,243,810 C→T 27.1% intergenic (+75/‑111) yqjK → / → yqjF uncharacterized protein/putative quinol oxidase subunit
RA 3,247,010 C→A 30.8% P188P (CCG→CCT yhaJ ← LysR family putative transcriptional regulator
RA 3,252,258 C→A 91.6% D251Y (GAT→TAT)  tdcG ← L‑serine dehydratase 3, anaerobic
RA 3,260,296 G→T 12.2% N43K (AAC→AAA tdcA ← tdc operon transcriptional activator
RA 3,263,460 C→A 10.6% intergenic (+499/+115) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,278,427 C→A 57.8% C128* (TGC→TGA agaC → N‑acetylgalactosamine‑specific enzyme IIC component of PTS
RA 3,302,027 G→T 81.1% S86* (TCG→TAG)  nlpI ← lipoprotein involved in osmotic sensitivity and filamentation
RA 3,309,359 C→A 5.8% T5T (ACG→ACT infB ← translation initiation factor IF‑2
RA 3,311,854 G→T 6.1% intergenic (‑206/‑142) metY ← / → argG tRNA‑Met/argininosuccinate synthetase
RA 3,314,944 G→A 8.4% T10I (ACT→ATT)  yhbX ← putative EptAB family phosphoethanolamine transferase, inner membrane protein
RA 3,354,405 C→T 88.7% intergenic (+430/‑130) gltD → / → gltF glutamate synthase, 4Fe‑4S protein, small subunit/periplasmic protein
RA 3,371,372 G→T 48.7% I65I (ATC→ATA rpsI ← 30S ribosomal subunit protein S9
RA 3,373,985 C→A 92.0% intergenic (+37/‑117) yhcB → / → degQ DUF1043 family inner membrane‑anchored protein/serine endoprotease, periplasmic
RA 3,375,260 G→T 86.3% D387Y (GAT→TAT)  degQ → serine endoprotease, periplasmic
RA 3,394,910 G→A 5.3% S594S (TCC→TCT csrD ← targeting factor for csrBC sRNA degradation
RA 3,396,082 G→T 78.5% R204S (CGT→AGT)  csrD ← targeting factor for csrBC sRNA degradation
RA 3,422,390 C→A 26.9% intergenic (‑269/‑205) rrsD ← / → yrdA 16S ribosomal RNA of rrnD operon/bacterial transferase hexapeptide domain protein
RA 3,440,151 G→T 74.8% I31I (ATC→ATA rpsN ← 30S ribosomal subunit protein S14
RA 3,440,444 G→T 92.6% S118S (TCC→TCA rplE ← 50S ribosomal subunit protein L5
RA 3,455,867 C→A 89.0% I55I (ATC→ATA gspJ → putative general secretory pathway component, cryptic
RA 3,471,787 C→A 14.3% D24Y (GAT→TAT)  slyD ← FKBP‑type peptidyl prolyl cis‑trans isomerase (rotamase)
RA 3,473,139 G→T 92.2% I276I (ATC→ATA kefB ← potassium:proton antiporter
RA 3,492,803 G→T 7.4% intergenic (+243/‑4) cysG → / → yhfL fused siroheme synthase 1,3‑dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase/small lipoprotein
RA 3,494,082 T→A 5.2% W272R (TGG→AGG)  frlA → putative fructoselysine transporter
RA 3,494,195 G→T 9.1% A309A (GCG→GCT frlA → putative fructoselysine transporter
RA 3,498,214 T→G 9.7% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,498,239 A→C 6.5% intergenic (+97/+55) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,519,657 G→T 100% intergenic (‑149/‑171) nudE ← / → yrfF adenosine nucleotide hydrolase; substrates include Ap3A, Ap2A, ADP‑ribose, NADH/inner membrane protein
RA 3,530,481 G→T 5.5% R104L (CGT→CTT)  greB → transcript cleavage factor
RA 3,543,959 G→T 86.5% A625E (GCG→GAG)  malP ← maltodextrin phosphorylase
RA 3,561,574 C→A 84.5% W372L (TGG→TTG)  glgC ← glucose‑1‑phosphate adenylyltransferase
RA 3,622,519 C→A 54.7% R127L (CGA→CTA)  rbbA ← ribosome‑associated ATPase: ATP‑binding protein/ATP‑binding membrane protein
RA 3,635,421 C→T 10.5% G400G (GGC→GGT dtpB → dipeptide and tripeptide permease B
RA 3,664,370 G→T 6.7% A473A (GCG→GCT treF → cytoplasmic trehalase
RA 3,675,072 A→C 8.1% Y77D (TAC→GAC)  yhjJ ← putative periplasmic M16 family chaperone
RA 3,679,301 T→G 78.2% Q1078P (CAG→CCG)  bcsC ← cellulose synthase subunit
RA 3,679,324 C→A 90.2% M1070I (ATG→ATT bcsC ← cellulose synthase subunit
RA 3,679,887 C→A 5.2% G883C (GGT→TGT)  bcsC ← cellulose synthase subunit
RA 3,681,219 C→A 56.1% A439S (GCG→TCG)  bcsC ← cellulose synthase subunit
RA 3,698,074 C→A 5.9% M16I (ATG→ATT dppC ← dipeptide/heme transporter
RA 3,721,485 T→G 9.9% Y416S (TAC→TCC)  xylB ← xylulokinase
RA 3,738,431 C→A 90.6% P90T (CCA→ACA)  yiaN → 2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
RA 3,774,382 T→G 11.8% intergenic (+5/‑193) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,785,915 G→T 91.5% intergenic (‑4/+271) kbl ← / ← yibB glycine C‑acetyltransferase/YibB family protein, function unknown
RA 3,810,195 C→A 90.3% R54S (CGT→AGT)  yicC → UPF0701 family protein
RA 3,816,601 A→T 100% H281L (CAC→CTC)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,818,010 C→A 92.0% H46N (CAT→AAT)  trmH → tRNA mG18‑2'‑O‑methyltransferase, SAM‑dependent
RA 3,822,340 A→C 14.8% Q12H (CAA→CAC xanP → xanthine permease
RA 3,822,345 T→G 7.4% V14G (GTT→GGT)  xanP → xanthine permease
RA 3,825,702 C→A 89.5% K732N (AAG→AAT yicI ← putative alpha‑glucosidase
RA 3,830,311 G→T 76.4% intergenic (+395/‑2) yicT → / → setC pseudogene/putative arabinose efflux transporter
RA 3,837,177 C→T 60.1% intergenic (‑28/‑147) yicO ← / → ade putative adenine permease/cryptic adenine deaminase
RA 3,839,562 C→A 6.4% A322A (GCG→GCT uhpT ← hexose phosphate transporter
RA 3,844,801 C→A 6.3% M448I (ATG→ATT ilvB ← acetolactate synthase 2 large subunit
RA 3,850,839 G→T 53.6% P309Q (CCG→CAG)  yidJ ← sulfatase/phosphatase superfamily protein
RA 3,860,159 C→A 87.1% P33P (CCG→CCT ibpB ← heat shock chaperone
RA 3,869,280 G→T 10.9% M161I (ATG→ATT yidX → putative lipoprotein
RA 3,870,977 T→A 7.1% intergenic (‑152/+88) yidB ← / ← gyrB DUF937 family protein/DNA gyrase, subunit B
RA 3,888,527 G→T 51.3% L172L (CTG→CTT chrR → chromate reductase, Class I, flavoprotein
RA 3,896,145 G→T 8.9% S306Y (TCC→TAC)  bglB ← cryptic phospho‑beta‑glucosidase B
RA 3,901,867 T→G 5.4% intergenic (‑141/+42) pstB ← / ← pstA phosphate transporter subunit/phosphate transporter subunit
RA 3,907,094 G→A 49.7% intergenic (‑66/+96) glmS ← / ← glmU L‑glutamine:D‑fructose‑6‑phosphate aminotransferase/fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase
RA 3,910,894 C→A 89.3% M244I (ATG→ATT atpG ← F1 sector of membrane‑bound ATP synthase, gamma subunit
RA 3,916,989 C→A 7.5% D18Y (GAT→TAT)  rsmG ← 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein
RA 3,919,832 T→A 11.8% intergenic (‑17/+73) mioC ← / ← asnC FMN‑binding protein MioC/transcriptional activator of asnA; autorepressor
RA 3,926,609 C→A 5.5% intergenic (+65/‑102) kup → / → rbsD potassium transporter/putative cytoplasmic sugar‑binding protein
RA 3,930,583 A→T 7.4% intergenic (+55/‑71) rbsB → / → rbsK D‑ribose transporter subunit/ribokinase
RA 3,952,842 A→C 5.8% intergenic (+37/+50) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,960,036 G→A 100% R87H (CGC→CAC)  rho → transcription termination factor
RA 3,994,247 A→T 92.6% V57E (GTG→GAG)  yigE ← DUF2233 family protein
RA 4,009,705 G→A 90.6% intergenic (‑176/‑86) ysgA ← / → udp putative carboxymethylenebutenolidase/uridine phosphorylase
RA 4,049,008 A→T 6.8% L231Q (CTG→CAG)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG
RA 4,056,018 T→A 94.4% F138I (TTC→ATC)  yihN → MFS transporter family protein
RA 4,062,424 G→T 85.2% I71I (ATC→ATA yihQ ← alpha‑glucosidase
RA 4,066,133 T→G 93.0% S267R (AGC→CGC)  yihU ← gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
RA 4,068,208 C→A 89.7% G60G (GGC→GGA yihW → DeoR family putative transcriptional regulator
RA 4,094,362 C→A 93.3% W181L (TGG→TTG)  cpxA ← sensory histidine kinase in two‑component regulatory system with CpxR
RA 4,097,822 G→T 75.9% E115* (GAA→TAA)  pfkA → 6‑phosphofructokinase I
RA 4,127,022 T→G 87.6% E188D (GAA→GAC yijF ← DUF1287 family protein
RA 4,142,369 A→T 6.2% Y220N (TAC→AAC)  ppc ← phosphoenolpyruvate carboxylase
RA 4,143,375 A→G 5.6% intergenic (‑349/+249) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,152,581 T→C 5.4% V206V (GTA→GTG trmA ← tRNA m(5)U54 methyltransferase, SAM‑dependent; tmRNA m(5)U341 methyltransferase
RA 4,161,800 A→C 7.0% intergenic (+116/‑185) rrfB → / → murB 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding
RA 4,173,276 A→T 7.6% T702S (ACT→TCT)  rpoB → RNA polymerase, beta subunit
RA 4,175,869 T→A 5.9% C198S (TGC→AGC)  rpoC → RNA polymerase, beta prime subunit
RA 4,176,823 G→T 73.7% D516Y (GAC→TAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,181,800 G→T 5.8% S255* (TCG→TAG)  thiG ← thiamine biosynthesis ThiGH complex subunit
RA 4,211,576 G→T 5.6% S280R (AGC→AGA arpA ← ankyrin repeat protein
RA 4,211,803 G→A 76.3% P205S (CCT→TCT)  arpA ← ankyrin repeat protein
RA 4,212,014 G→T 6.5% F134L (TTC→TTA arpA ← ankyrin repeat protein
RA 4,230,913 A→T 76.5% W424R (TGG→AGG)  xylE ← D‑xylose transporter
RA 4,273,158 A→T 5.7% intergenic (‑155/+23) yjcF ← / ← actP pentapeptide repeats protein/acetate transporter
RA 4,290,080 G→T 80.7% I256I (ATC→ATA mdtP ← outer membrane factor of efflux pump
RA 4,291,537 G→T 6.3% I453I (ATC→ATA mdtO ← membrane translocase (MDR) of MdtNOP efflux pump, PET family
RA 4,295,721 A→G 6.4% Y232H (TAC→CAC)  yjcS ← metallo‑beta‑lactamase superfamily protein
RA 4,297,846 C→A 100% M150I (ATG→ATT alsE ← allulose‑6‑phosphate 3‑epimerase
RA 4,303,256 G→T 5.5% V30V (GTG→GTT rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,303,614 T→A 78.8% *150R (TGA→AGA)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,306,969 T→A 57.3% N23Y (AAC→TAC)  phnM ← ribophosphonate triphosphate hydrolase
RA 4,312,246 C→A 54.4% intergenic (‑7/+14) phnF ← / ← phnE putative DNA‑binding transcriptional regulator of phosphonate uptake and biodegradation/defective phosphonate ABC transporter permease;transport; Transport of small molecules: Anions; membrane channel protein component of Pn transporter; membrane component of ABC superfamily
RA 4,315,765 A→G 5.7% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,373,326 C→A 5.4% V289V (GTC→GTA epmA → Elongation Factor P Lys34 lysyltransferase
RA 4,382,288 A→C 5.2% intergenic (+36/‑1) glyV → / → glyX tRNA‑Gly/tRNA‑Gly
RA 4,382,806 T→A 49.8% Q360L (CAA→CTA)  queG ← epoxyqueuosine reductase, cobalamine‑stimulated; queosine biosynthesis
RA 4,383,318 G→T 8.1% V189V (GTC→GTA queG ← epoxyqueuosine reductase, cobalamine‑stimulated; queosine biosynthesis
RA 4,388,902 G→T 89.1% A558A (GCG→GCT mutL → methyl‑directed mismatch repair protein
RA 4,396,598 A→T 87.9% E43V (GAA→GTA)  rnr → exoribonuclease R, RNase R
RA 4,436,661 C→A 11.6% N911K (AAC→AAA tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,458,254 T→A 89.3% V271V (GTT→GTA mgtA → magnesium transporter
RA 4,458,609 G→T 91.5% D390Y (GAT→TAT)  mgtA → magnesium transporter
RA 4,464,572 G→T 58.2% intergenic (+38/‑107) bdcR → / → tabA transcriptional repressor for divergent bdcA/biofilm modulator regulated by toxins; DUF386 family protein, cupin superfamily
RA 4,469,550 G→A 78.6% A2T (GCT→ACT)  yjgN → DUF898 family inner membrane protein
RA 4,470,017 G→A 93.3% M157I (ATG→ATA yjgN → DUF898 family inner membrane protein
RA 4,489,542 A→T 48.9% pseudogene (810/942 nt) yjgX ← pseudogene fragment
RA 4,495,002 C→A 11.9% F376L (TTC→TTA yjhB → putative transporter
RA 4,510,428 G→T 6.4% intergenic (‑287/+60) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,511,877 G→A 52.1% intergenic (‑40/+67) yjhF ← / ← yjhG putative transporter/putative dehydratase
RA 4,512,693 T→A 86.4% K407* (AAG→TAG)  yjhG ← putative dehydratase
RA 4,525,234 G→A 90.1% pseudogene (415/1029 nt) yjhR → pseudogene, helicase family; putative frameshift suppressor
RA 4,531,143 A→T 90.1% I124F (ATT→TTT)  fimB → tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,546,084 G→A 5.8% H18H (CAC→CAT yjiC ← uncharacterized protein
RA 4,556,296 C→T 57.8% Q256Q (CAG→CAA yjiN ← zinc‑type alcohol dehydrogenase‑like protein
RA 4,562,043 C→A 88.0% intergenic (+311/‑188) yjiS → / → yjiT DUF1127 family protein/pseudogene
RA 4,584,381 G→T 89.0% I53I (ATC→ATA yjjL ← L‑galactonate transporter
RA 4,601,014 T→C 51.9% intergenic (+194/‑199) prfC → / → osmY peptide chain release factor RF‑3/periplasmic protein
RA 4,606,972 G→T 9.7% intergenic (‑90/‑168) yjjI ← / → deoC DUF3029 family protein, putative glycine radical enzyme/2‑deoxyribose‑5‑phosphate aldolase, NAD(P)‑linked
RA 4,626,235 G→T 52.6% E138* (GAA→TAA)  creB → response regulator in two‑component regulatory system with CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 373854 375109 1256 186 [184] [182] 186 frmA–[frmR] frmA,[frmR]
* * ÷ CP009273 4100605 4101526 922 187 [185] [183] 186 [cdh]–tpiA [cdh],tpiA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =245 (0.820)245 (0.820) 140/276 NT 50.0% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 360278NA (NA)7819 (27.850)
+TTAATTAA
197/260 NT 96.2% intergenic (‑212/+383) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 361823 325 (1.090)coding (831/834 nt) mhpR mhp operon transcriptional activator
* ? CP009273 360652 =355 (1.190)6912 (34.900)
+46 bp
184/184 NT 97.1% intergenic (‑586/+9) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 1317577 260 (0.870)coding (101/882 nt) yciV PHP domain protein
* ? CP009273 376716 =NA (NA)124 (0.420) 83/276 NT 53.7% intergenic (‑1/‑91) yaiX/insC1 pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase/IS2 repressor TnpA
?CP009273 = 386403 107 (0.360)pseudogene (697/1458 nt) yaiT pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
* ? CP009273 376716 =NA (NA)275 (0.920) 136/276 NT 96.5% intergenic (‑1/‑91) yaiX/insC1 pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase/IS2 repressor TnpA
?CP009273 566411 = 10 (0.030)coding (63/552 nt) ybcL inactive polymorphonuclear leukocyte migration suppressor; DLP12 prophage; secreted protein, UPF0098 family
* ? CP009273 = 378046NA (NA)162 (0.540) 101/276 NT 60.4% intergenic (+11/+1) insD1/yaiX IS2 transposase TnpB/pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
?CP009273 386399 = 106 (0.360)pseudogene (693/1458 nt) yaiT pseudogene, autotransporter family;putative structure; Not classified; interrupted by IS3; putative flagellin structural protein
* ? CP009273 = 378046NA (NA)251 (0.840) 132/276 NT 96.5% intergenic (+11/+1) insD1/yaiX IS2 transposase TnpB/pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
?CP009273 = 566415 9 (0.030)coding (67/552 nt) ybcL inactive polymorphonuclear leukocyte migration suppressor; DLP12 prophage; secreted protein, UPF0098 family
* ? CP009273 776825 =188 (0.630)23 (0.080)
+G
3/274 NT 9.5% noncoding (1/76 nt) lysZ tRNA‑Lys
?CP009273 = 2514487 255 (0.860)noncoding (215/1345 nt) IS186 repeat region
* ? CP009273 1203246 =19 (0.060)199 (0.760) 115/244 NT 94.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 5 (0.020)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326120 (0.070)300 (1.140) 128/244 NT 96.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 5 (0.020)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 1635219 =22 (0.070)256 (0.870) 126/272 NT 92.1% intergenic (‑377/+377) essQ/cspB Qin prophage; putative S lysis protein/Qin prophage; cold shock protein
?CP009273 = 2063752 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? CP009273 = 163522323 (0.080)192 (0.640) 111/276 NT 89.3% intergenic (‑381/+373) essQ/cspB Qin prophage; putative S lysis protein/Qin prophage; cold shock protein
?CP009273 = 1645805 NA (NA)intergenic (+11/‑3) insD1/intQ IS2 transposase TnpB;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related/pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
* ? CP009273 = 2811301220 (0.740)15 (0.060) 4/236 NT 6.9% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?CP009273 2811851 = 217 (0.850)noncoding (58/77 nt) argV tRNA‑Arg
* ? CP009273 3179455 =NA (NA)17 (0.060) 13/276 NT 5.9% intergenic (+7/‑91) yqiG/insC1 pseudogene; fimbrial export usher family;putative membrane; Not classified; putative membrane protein/IS2 repressor TnpA
?CP009273 = 3360465 269 (0.900)coding (181/717 nt) yhcF putative transcriptional regulator