breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 4,237 A→G K168K (AAA→AAG thrC → threonine synthase
RA 5,201 A→G intergenic (+181/‑33) thrC → / → yaaX threonine synthase/DUF2502 domain‑containing protein YaaX
RA 9,927 A→C intergenic (+34/+1) mog → / ← satP molybdopterin adenylyltransferase/acetate/succinate:H(+) symporter
RA 58,877 A→G N135S (AAT→AGT)  yabP → putative uncharacterized protein YabP
RA 92,197 C→T A262V (GCC→GTC)  ftsI → peptidoglycan DD‑transpeptidase FtsI
RA 111,230 (C)6→7 coding (187/390 nt) mutT → 8‑oxo‑dGTP diphosphatase
RA 132,902 T→C C430R (TGC→CGC)  acnB → hypothetical protein
RA 164,927 A→C L66L (CTA→CTC mrcB → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
RA 166,957 A→C Y743S (TAT→TCT)  mrcB → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
RA 168,090 A→G T203A (ACT→GCT)  fhuA → ferrichrome outer membrane transporter/phage receptor
RA 188,652 G→T intergenic (‑2/+60) glnD ← / ← map PII uridylyltransferase/uridylyl removing enzyme/methionine aminopeptidase
RA 202,482 A→C K128Q (AAA→CAA)  fabZ → 3‑hydroxy‑acyl‑[acyl‑carrier‑protein] dehydratase
RA 205,071 A→G A193A (GCA→GCG rnhB → RNase HII
RA 249,082 G→A pseudogene (989/1713 nt) lfhA ← flagellar system protein, promoterless fragment
RA 249,545 T→C pseudogene (526/1713 nt) lfhA ← flagellar system protein, promoterless fragment
RA 256,406 G→C V144L (GTA→CTA)  gpt → xanthine‑guanine phsophoribosyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 277,906 A→T Y299* (TAT→TAA afuC ← CP4‑6 prophage; ABC transporter ATP‑binding protein AfuC
RA 283,510 A→G T104A (ACC→GCC)  yagF → CP4‑6 prophage; D‑xylonate dehydratase
RA 308,054 (C)7→8 coding (1279/2526 nt) ecpC ← putative fimbrial usher protein EcpC
RA 327,519 A→C I405M (ATT→ATG betB ← betaine aldehyde dehydrogenase
RA 328,751 T→G D195A (GAT→GCT)  betI ← DNA‑binding transcriptional repressor BetI
RA 362,014 G→A A389V (GCG→GTG)  lacY ← lactose permease
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 417,935 T→C L16P (CTT→CCT)  phoR → sensory histidine kinase PhoR
RA 423,231 G→A Q238Q (CAG→CAA malZ → maltodextrin glucosidase
RA 431,181 T→C N278S (AAC→AGC)  tsx ← nucleoside‑specific channel‑forming protein Tsx
RA 474,228 C→T H421H (CAC→CAT amtB → ammonia/ammonium transporter
RA 485,852 C→T S31F (TCC→TTC)  acrR → DNA‑binding transcriptional repressor AcrR
RA 501,943 (C)5→6 coding (1296/1677 nt) ybaL ← putative transporter YbaL
RA 510,996 A→C T128T (ACT→ACG copA ← Cu(+) exporting P‑type ATPase
RA 514,711 G→A P50L (CCG→CTG)  ybbJ ← NfeD‑like family protein
RA 522,457 T→G L681R (CTG→CGG)  ybbP → putative ABC transporter membrane subunit YbbP
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 573,777 (G)5→4 coding (48/141 nt) ylcG → DLP12 prophage; uncharacterized protein YlcG
RA 581,288 A→C K152T (AAA→ACA)  nohD → DLP12 prophage; putative DNA‑packaging protein NohD
RA 656,095 A→C intergenic (‑127/‑462) dcuC ← / → pagP anaerobic C4‑dicarboxylate transporter DcuC/Lipid IVA palmitoyltransferase
RA 666,217 G→T L33M (CTG→ATG)  mrdB ← SEDS family protein MrdB
RA 669,834 (G)6→7 coding (74/612 nt) cobC ← putative adenosylcobalamin phosphatase/alpha‑ribazole phosphatase
RA 669,942 A→G L211L (TTG→CTG)  nadD ← nicotinate‑mononucleotide adenylyltransferase
RA 705,972 (T)9→8 intergenic (+82/‑121) nagE → / → glnS N‑acetylglucosamine specific PTS enzyme IIABC component/glutamine‑‑tRNA ligase
RA 742,864 A→G K13R (AAA→AGA)  ybgI → radiation resistance protein YbgI
RA 744,100 A→G T170A (ACT→GCT)  pxpB → 5‑oxoprolinase component B
RA 752,258 T→G N180H (AAC→CAC)  ybgD ← putative fimbrial protein YbgD
RA 784,092 T→G V244V (GTA→GTC zitB ← Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
RA 786,340 T→C G236G (GGT→GGC aroG → 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive
RA 792,328 (T)5→4 coding (1461/1473 nt) modF ← ABC family protein ModF
RA 817,869 T→C F276L (TTT→CTT)  moaA → GTP 3',8'‑cyclase
RA 830,378 T→C Q89R (CAG→CGG)  cecR ← DNA‑binding transcriptional dual regulator CecR
RA 858,226 A→G V279A (GTG→GCG)  ybiU ← DUF1479 domain‑containing protein YbiU
RA 874,796 A→G R606R (AGA→AGG pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 890,526 A→G A76A (GCT→GCC grxA ← reduced glutaredoxin 1
RA 898,332 A→G Y115C (TAC→TGC)  ybjO → putative inner membrane protein
RA 917,539 T→G L356W (TTG→TGG)  ybjD → DUF2813 domain‑containing protein YbjD
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 934,042 T→G D273E (GAT→GAG ftsK → cell division DNA translocase FtsK
RA 956,500 A→G S45P (TCT→CCT)  ycaO ← ribosomal protein S12 methylthiotransferase accessory factor YcaO
RA 958,854 A→G T15A (ACT→GCT)  aroA → 3‑phosphoshikimate 1‑carboxyvinyltransferase
RA 981,308 C→T P88S (CCG→TCG)  ldtD → L,D‑transpeptidase LdtD
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,011,270 C→T T436M (ACG→ATG)  uup → ATP‑binding protein with possible role in replication
RA 1,044,474 G→T A68D (GCC→GAC)  etp ← phosphotyrosine‑protein phosphatase
RA 1,048,748 C→T R195R (CGG→CGA gfcB ← lipoprotein GfcB
RA 1,051,398 C→T intergenic (‑223/‑63) cspH ← / → cspG CspA family protein CspH/cold shock protein CspG
RA 1,053,497 T→C E894E (GAA→GAG torS ← sensory histidine kinase TorS
RA 1,059,436 (G)6→7 coding (181/2547 nt) torA → trimethylamine N‑oxide reductase 1
RA 1,079,319 A→G T5T (ACA→ACG putP → proline:Na(+) symporter
RA 1,081,167 A→C intergenic (+354/‑189) putP → / → efeU proline:Na(+) symporter/inactive ferrous iron permease EfeU, N‑terminal fragment
RA 1,106,285 T→C F156L (TTT→CTT)  ymdB → 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,191,861 A→G R42R (CGT→CGC purB ← adenylosuccinate lyase
RA 1,192,954 C→T A274T (GCT→ACT)  mnmA ← tRNA‑specific 2‑thiouridylase
RA 1,208,003 A→G N168S (AAT→AGT)  ycfK → e14 prophage; protein StfP
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,230,015 A→G L127P (CTG→CCG)  hlyE ← hemolysin E
RA 1,248,707 A→G G136G (GGT→GGC dhaM ← dihydroxyacetone kinase subunit M
RA 1,314,972 C→T G85S (GGT→AGT)  yciE ← DUF892 domain‑containing protein YciE
RA 1,363,657 A→C G305G (GGA→GGC puuC → gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
RA 1,406,519 C→T R181C (CGC→TGC)  smrA → DNA endonuclease SmrA
RA 1,417,350 T→G K13Q (AAA→CAA)  recE ← Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
RA 1,454,938 T→C V24A (GTT→GCT)  paaB → phenylacetyl‑CoA 1,2‑epoxidase subunit B
RA 1,487,050 T→G intergenic (+87/‑185) hrpA → / → ydcF ATP‑dependent RNA helicase HrpA/DUF218 domain‑containing protein YdcF
RA 1,511,133 T→C R377R (CGT→CGC ydcR → fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
RA 1,511,751 A→G E33G (GAA→GGA)  ydcS → putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
RA 1,535,078 G→A R261C (CGT→TGT)  yddE ← PF02567 family protein YddE
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,550,077 A→G T893A (ACG→GCG)  fdnG → formate dehydrogenase N subunit alpha
RA 1,568,689 T→C P45P (CCA→CCG yddW ← putative lipoprotein YddW
RA 1,577,522 A→C M33R (ATG→AGG)  yddB ← putative TonB‑dependent receptor
RA 1,585,101 (C)6→7 coding (1386/2280 nt) ydeP ← putative oxidoreductase YdeP
RA 1,590,491 T→C pseudogene (46/203 nt) yneL ← putative HTH‑type transcriptional regulator YneL
JC 1,594,256 (C)8→11 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,612,466 A→C V262V (GTT→GTG glsB ← glutaminase 2
RA 1,631,808 C→A A160S (GCG→TCG)  ydfJ ← putative transporter YdfJ
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,635,269 C→T A193A (GCG→GCA ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,293 G→A T185T (ACC→ACT ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,296 T→G G184G (GGA→GGC ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,322 C→T E176K (GAA→AAA)  ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,635,335 C→T T171T (ACG→ACA ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,666,361 A→G E349E (GAA→GAG clcB → putative chloride:H(+) antiporter ClcB
RA 1,667,893 (C)5→6 coding (672/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,677,049 T→C A285A (GCA→GCG pntA ← pyridine nucleotide transhydrogenase subunit alpha
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,700,138 A→G T262A (ACC→GCC)  malX → PTS enzyme IIBC component MalX
RA 1,730,251 A→G I389V (ATT→GTT)  lhr → putative ATP‑dependent helicase Lhr
RA 1,747,140 A→C I4L (ATT→CTT)  ydhS → FAD/NAD(P) binding domain‑containing protein YdhS
RA 1,762,442 A→G V24A (GTT→GCT)  sufC ← Fe‑S cluster scaffold complex subunit SufC
RA 1,767,223 A→C L488R (CTG→CGG)  ydiJ ← putative FAD‑linked oxidoreductase
RA 1,769,478 (G)7→8 coding (405/1113 nt) ydiK → putative transporter YdiK
RA 1,770,656 C→T A14A (GCC→GCT ydiL → DUF1870 domain‑containing protein YdiL
RA 1,814,705 T→G I280S (ATT→AGT)  katE → catalase II
RA 1,815,057 G→A V397V (GTG→GTA katE → catalase II
RA 1,828,768 A→G S323P (TCT→CCT)  astD ← aldehyde dehydrogenase
RA 1,842,177 T→G intergenic (‑42/‑194) ynjH ← / → gdhA DUF1496 domain‑containing protein YnjH/glutamate dehydrogenase
RA 1,853,832 T→C S50G (AGC→GGC)  ydjE ← putative transporter YdjE
RA 1,865,059 T→C Y193C (TAC→TGC)  yeaE ← methylglyoxal reductase YeaE
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,878,841 (T)6→5 coding (69/99 nt) yoaK → putative membrane protein YoaK
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,887,685 T→C T103A (ACG→GCG)  rnd ← RNase D
RA 1,894,366 T→C R78R (CGT→CGC yoaC → DUF1889 domain‑containing protein YoaC
RA 1,928,390 T→C R150R (CGA→CGG ptrB ← oligopeptidase B
RA 1,942,858 A→C K66T (AAA→ACA)  znuC → Zn(2(+)) ABC transporter ATP binding subunit
RA 1,945,078 C→T K93K (AAG→AAA ruvB ← Holliday junction branch migration complex subunit RuvB
RA 1,954,604 A→G F802L (TTT→CTT)  torZ ← trimethylamine N‑oxide reductase 2
RA 1,968,611 G→T L252I (CTT→ATT)  cheR ← chemotaxis protein methyltransferase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,987,434 A→G intergenic (+6/+73) ftnB → / ← yecJ putative ferritin‑like protein/DUF2766 domain‑containing protein YecJ
RA 1,999,707 C→A A227S (GCG→TCG)  tcyJ ← cystine ABC transporter periplasmic binding protein
RA 2,001,157 G→A G211G (GGC→GGT fliA ← RNA polymerase, sigma 28 (sigma F) factor
RA 2,003,791 (A)8→7 intergenic (‑185/‑81) fliC ← / → fliD flagellar filament structural protein/flagellar filament capping protein
RA 2,031,817 T→C E167E (GAA→GAG dcm ← DNA‑cytosine methyltransferase
RA 2,066,253 A→G intergenic (‑489/+52) cobU ← / ← insH‑6 cobinamide‑P guanylyltransferase/cobinamide kinase/CP4‑44 prophage; IS5 transposase and trans‑activator
RA 2,090,243 A→C S18R (AGT→CGT)  hisG → ATP phosphoribosyltransferase
RA 2,110,387 T→C G211G (GGA→GGG rfbA ← dTDP‑glucose pyrophosphorylase
RA 2,114,146 T→C N61S (AAC→AGC)  galF ← UTP:glucose‑1‑phosphate uridylyltransferase, low activity
RA 2,139,083 C→T A394A (GCC→GCT yegH → inner membrane protein YegH
RA 2,152,194 A→G R93R (CGT→CGC pphC ← protein‑serine/threonine phosphatase PphC
RA 2,172,654 C→T D74N (GAT→AAT)  gatD ← galactitol‑1‑phosphate 5‑dehydrogenase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,194,757 A→T D153V (GAT→GTT)  metG → methionine‑‑tRNA ligase
RA 2,201,704 A→G S476G (AGC→GGC)  yehI → DUF4132 domain‑containing protein YehI
RA 2,227,340 (C)6→7 pseudogene (1419/1436 nt) mdtQ ← putative multidrug resistance outer membrane protein MdtQ
RA 2,229,870 T→C K172K (AAA→AAG dusC ← tRNA‑dihydrouridine(16) synthase
RA 2,268,972 A→G Y40C (TAT→TGT)  lpxT → Kdo2‑lipid A phosphotransferase
RA 2,277,598 T→C R433R (CGT→CGC yejF → putative oligopeptide ABC transporter ATP binding subunit
RA 2,337,723 A→C G566G (GGT→GGG gyrA ← DNA gyrase subunit A
RA 2,371,433 (G)5→4 coding (526/1653 nt) arnT → lipid IVA 4‑amino‑4‑deoxy‑L‑arabinosyltransferase
RA 2,389,175 Δ1 bp coding (60/849 nt) yfbP → uncharacterized protein YfbP
RA 2,433,011 A→G intergenic (‑69/+1) folC ← / ← accD bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase/acetyl‑CoA carboxyltransferase subunit beta
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,454,187 A→C pseudogene (815/2646 nt) yfcU ← putative outer membrane usher protein YfcU
RA 2,462,880 T→C intergenic (+234/‑132) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,559,952 G→A R365R (AGG→AGA intZ → CPZ‑55 prophage; putative phage integrase IntZ
RA 2,564,783 T→C G87G (GGT→GGC yffS → CPZ‑55 prophage; uncharacterized protein YffS
RA 2,606,743 G→A G161D (GGC→GAC)  hyfE → hydrogenase 4 component E
RA 2,626,828 T→C Y704C (TAC→TGC)  pdeF ← cyclic di‑GMP phosphodiesterase PdeF
RA 2,635,685 A→G L44L (TTA→CTA)  yfgJ ← zinc ribbon domain‑containing protein YfgJ
RA 2,669,362 T→C S111P (TCT→CCT)  hcaE → putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
RA 2,671,917 Δ1 bp coding (693/813 nt) hcaB → 2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
RA 2,721,756 G→A G602S (GGT→AGT)  patZ → peptidyl‑lysine acetyltransferase
RA 2,752,917 C→G L375V (CTT→GTT)  recN → DNA repair protein RecN
RA 2,753,762 A→C Q53P (CAA→CCA)  bamE → outer membrane protein assembly factor BamE
RA 2,762,713 T→C T276T (ACA→ACG abpB ← CP4‑57 prophage; putative helicase YfjK
RA 2,765,794 (C)7→8 intergenic (‑18/‑124) yfjM ← / → rnlA CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,775,273 T→C intergenic (+252/‑272) yfjW → / → ypjI CP4‑57 prophage; uncharacterized protein YfjW/putative UPF0401 protein YpjI
RA 2,810,164 C→T A183V (GCA→GTA)  ygaZ → L‑valine exporter subunit YgaZ
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,812,082 T→C I219T (ATC→ACC)  emrA → multidrug efflux pump membrane fusion protein EmrA
RA 2,819,780 T→C K744K (AAA→AAG alaS ← alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,825,817 C→A intergenic (‑241/‑15) mltB ← / → srlA membrane‑bound lytic murein transglycosylase B/sorbitol‑specific PTS enzyme IIC2 component
RA 2,829,365 G→A V107I (GTT→ATT)  srlR → DNA‑binding transcriptional repressor SrlR
RA 2,831,712 C→T G193D (GGC→GAC)  norR ← DNA‑binding transcriptional dual regulator NorR
RA 2,842,850 T→G D65A (GAT→GCT)  hycI ← hydrogenase 3 maturation protease
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,896,787 T→C S362G (AGT→GGT)  ygcS ← putative transporter YgcS
RA 2,905,219 (C)6→8 coding (200/672 nt) queE ← putative 7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,905,733 T→G F8C (TTC→TGC)  ygcG → protein YgcG
RA 2,918,126 G→A G420G (GGC→GGT gudD ← D‑glucarate dehydratase
RA 2,922,549 T→G T146P (ACG→CCG)  yqcA ← putative flavodoxin YqcA
RA 2,942,466 T→C Q34Q (CAA→CAG gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,955,714 T→C Y97C (TAC→TGC)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,995,816 T→C pseudogene (205/707 nt) ygeN ← putative type III secretion system protein
RA 3,008,544 C→T G345G (GGC→GGT ygeX → 2,3‑diaminopropionate ammonia‑lyase
RA 3,009,043 G→A D94N (GAC→AAC)  ygeY → putative peptidase YgeY
RA 3,025,943 A→C I60L (ATT→CTT)  guaD → guanine deaminase
RA 3,038,560 A→G L88S (TTA→TCA)  dsbC ← protein disulfide isomerase DsbC
RA 3,054,895 G→A P184S (CCA→TCA)  ygfB ← UPF0149 family protein YgfB
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,096,766 T→C V29A (GTG→GCG)  rdgB → dITP/XTP pyrophosphatase
RA 3,101,039 (C)7→9 coding (585/720 nt) yggN ← putative EcfF
RA 3,103,600 +A coding (588/1053 nt) mutY → adenine DNA glycosylase
RA 3,104,918 T→C R162R (CGT→CGC mltC → membrane‑bound lytic murein transglycosylase C
RA 3,109,283 T→C intergenic (‑128/‑270) speC ← / → yqgA ornithine decarboxylase, biosynthetic/DUF554 domain‑containing protein YqgA
RA 3,119,228 A→G intergenic (‑116/+369) yghJ ← / ← glcA putative lipoprotein YghJ/glycolate/lactate:H(+) symporter GlcA
RA 3,142,721 T→C Y90C (TAC→TGC)  hybC ← hydrogenase 2 large subunit
RA 3,143,320 T→C D282G (GAC→GGC)  hybB ← hydrogenase 2 membrane subunit
RA 3,156,480 T→C L376L (TTG→CTG)  yqhD → NADPH‑dependent aldehyde reductase YqhD
RA 3,193,178 A→C V105V (GTA→GTC yqiK → protein YqiK
RA 3,200,134 T→C T278A (ACT→GCT)  ygiF ← inorganic triphosphatase
RA 3,216,481 (G)6→5 coding (11/765 nt) nfeF ← NADPH‑dependent ferric chelate reductase
RA 3,224,676 C→A R682S (CGC→AGC)  ebgA → evolved beta‑D‑galactosidase subunit alpha
RA 3,240,984 (G)7→9 coding (1041/1245 nt) sstT → serine/threonine:Na(+) symporter
RA 3,260,758 T→C D554G (GAC→GGC)  tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
RA 3,299,534 T→G N128H (AAC→CAC)  yhbP ← putative FMN binding protein YhbP
RA 3,304,813 (C)7→6 coding (1005/1245 nt) mtr ← tryptophan:H(+) symporter Mtr
RA 3,309,390 A→G G593G (GGT→GGC pnp ← polynucleotide phosphorylase
RA 3,323,624 G→A S149S (AGC→AGT glmM ← phosphoglucosamine mutase
RA 3,332,241 G→A V165V (GTC→GTT yhbE ← inner membrane protein YhbE
RA 3,344,248 T→C V102A (GTA→GCA)  lptB → lipopolysaccharide transport system ATP binding protein
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,356,708 T→G F662V (TTC→GTC)  gltB → glutamate synthase subunit GltB
RA 3,372,139 T→G S146R (AGT→CGT)  nanT ← N‑acetylneuraminate:H(+) symporter
RA 3,379,381 T→C N206S (AAC→AGC)  zapE ← cell division factor ZapE with ATPase activity
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,411,752 G→A V34I (GTC→ATC)  yhdJ → DNA adenine methyltransferase
RA 3,451,194 G→A G57G (GGC→GGT rplB ← 50S ribosomal subunit protein L2
RA 3,480,828 T→C T112A (ACC→GCC)  kefG ← protein required for KefB activity
RA 3,519,256 T→C intergenic (‑192/+209) aroK ← / ← hofQ shikimate kinase 1/DNA utilization protein HofQ
RA 3,522,408 T→C D115G (GAC→GGC)  hofM ← DNA utilization protein HofM
RA 3,540,301 G→A D47N (GAC→AAC)  feoA → ferrous iron transport protein A
RA 3,544,959 T→C C26C (TGT→TGC yhgH → DNA utilization protein YhgH
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,567,257 T→C L259L (TTA→TTG glgA ← glycogen synthase
RA 3,571,824 A→C F560C (TTC→TGC)  glgB ← 1,4‑alpha‑glucan branching enzyme
RA 3,577,903 T→C H275R (CAC→CGC)  gntR ← DNA‑binding transcriptional repressor GntR
RA 3,579,267 T→C T127A (ACG→GCG)  yhhW ← quercetin 2,3‑dioxygenase
RA 3,582,299 T→C F146S (TTC→TCC)  yhhZ → putative endonuclease YhhZ
RA 3,590,928 A→G intergenic (‑17/+81) ugpA ← / ← ugpB sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpA/sn‑glycerol 3‑phosphate ABC transporter periplasmic binding protein
RA 3,608,682 T→C intergenic (+33/+69) zntA → / ← tusA Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase/sulfur transfer protein TusA
RA 3,645,004 T→C D60G (GAC→GGC)  prlC ← oligopeptidase A
RA 3,723,278 A→C W374G (TGG→GGG)  glyS ← glycine‑‑tRNA ligase subunit beta
RA 3,749,644 G→A W138* (TGG→TAG)  sgbU → putative L‑xylulose 5‑phosphate 3‑epimerase
RA 3,757,772 A→C L19W (TTG→TGG)  yiaY ← L‑threonine dehydrogenase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,838,052 T→C I367T (ATC→ACC)  setC → putative arabinose exporter
RA 3,842,237 C→A E56* (GAA→TAA)  yicN ← conserved protein YicN
RA 3,846,324 A→G S282P (TCA→CCA)  uhpT ← hexose‑6‑phosphate:phosphate antiporter
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,866,463 A→G intergenic (‑190/+6) yidE ← / ← ibpB putative transport protein YidE/small heat shock protein IbpB
RA 3,884,194 A→C intergenic (+88/‑142) ysdD → / → rpmH protein YsdD/50S ribosomal subunit protein L34
RA 3,920,598 T→C E97E (GAA→GAG atpF ← ATP synthase Fo complex subunit b
RA 3,920,613 A→C A92A (GCT→GCG atpF ← ATP synthase Fo complex subunit b
RA 4,008,263 C→T S169S (AGC→AGT rhtC → L‑threonine exporter
RA 4,042,023 G→C intergenic (‑24/‑46) mobA ← / → yihD molybdenum cofactor guanylyltransferase/conserved protein YihD
RA 4,060,280 (T)9→8 intergenic (+50/‑167) bipA → / → yihL ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL
RA 4,060,875 A→G E143E (GAA→GAG yihL → putative transcriptional regulator YihL
RA 4,073,698 A→C intergenic (‑127/‑41) yihU ← / → yihV 3‑sulfolactaldehyde reductase/6‑deoxy‑6‑sulfofructose kinase
RA 4,090,640 C→T V96I (GTA→ATA)  frvX ← peptidase M42 family protein
RA 4,101,728 (G)7→8 coding (39/984 nt) kdgT → 2‑dehydro‑3‑deoxy‑D‑gluconate:H(+) symporter
RA 4,103,311 T→C S164P (TCC→CCC)  yiiM → 2‑amino‑6‑N‑hydroxylaminopurine resistance protein
RA 4,177,658 A→G T101A (ACC→GCC)  secE → Sec translocon subunit SecE
RA 4,205,209 A→C D241E (GAT→GAG purD ← phosphoribosylamine‑‑glycine ligase
RA 4,262,370 G→A E234E (GAG→GAA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,300,748 A→G S95P (TCC→CCC)  mdtP ← putative multidrug efflux pump outer membrane channel
RA 4,329,752 A→G E131E (GAA→GAG yjcZ → uncharacterized protein YjcZ
RA 4,338,406 A→G S706P (TCT→CCT)  adiA ← arginine decarboxylase, degradative
RA 4,355,554 A→G S286S (AGT→AGC dtpC ← dipeptide/tripeptide:H(+) symporter DtpC
RA 4,359,871 G→A A54V (GCG→GTG)  cadB ← lysine:cadaverine antiporter
RA 4,362,343 A→G intergenic (‑409/+208) cadC ← / ← pheU DNA‑binding transcriptional activator CadC/tRNA‑Phe
RA 4,389,226 G→A L49F (CTC→TTC)  mscM ← miniconductance mechanosensitive channel MscM
RA 4,397,834 G→A V141V (GTG→GTA mutL → DNA mismatch repair protein MutL
RA 4,404,529 A→G G48G (GGA→GGG yjeT → DUF2065 domain‑containing protein YjeT
RA 4,413,295 A→G S90G (AGC→GGC)  yjfC → putative acid‑‑amine ligase YjfC
RA 4,418,632 A→G Y332H (TAC→CAC)  ulaG ← L‑ascorbate‑6‑phosphate lactonase
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,474,725 C→T intergenic (+8/‑137) bdcR → / → tabA putative transcriptional regulator BdcR/DUF386 domain‑containing toxin‑antitoxin biofilm protein TabA
RA 4,480,876 T→G F383V (TTC→GTC)  yjgN → conserved inner membrane protein YjgN
RA 4,488,579 (C)7→8 coding (1485/1503 nt) yjgR ← DUF853 domain‑containing protein YjgR
RA 4,516,115 C→T R188H (CGC→CAC)  fecA ← ferric citrate outer membrane transporter
RA 4,525,117 A→G N229N (AAT→AAC yjhI ← KpLE2 phage‑like element; putative DNA‑binding transcriptional regulator YjhI
RA 4,540,613 A→G intergenic (‑1112/‑344) nanC ← / → fimB N‑acetylneuraminic acid outer membrane channel/regulator for fimA
RA 4,551,114 (C)5→6 coding (183/1344 nt) gntP ← fructuronate transporter
RA 4,563,850 T→C M26V (ATG→GTG)  ytiA ← uncharacterized protein YtiA
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,608,076 A→G T92A (ACT→GCT)  holD → DNA polymerase III subunit psi
RA 4,621,161 C→T G93G (GGC→GGT deoD → purine nucleoside phosphorylase
RA 4,627,920 C→A T202T (ACC→ACA nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 433032 433101 70 7 [4] [2] 6 nrdR NrdR transcriptional repressor
* * ÷ NC_000913 730239–730416 730416 1–178 6 [4] [5] 9 rhsC rhs element protein RhsC
* * ÷ NC_000913 730553–730565 731113–731058 494–561 10 [1] [4] 6 rhsC rhs element protein RhsC
* * ÷ NC_000913 1432756–1433198 1434232–1434077 880–1477 7 [3] [5] 6 [tfaR]–ynaE [tfaR],pinR,ynaE
* * ÷ NC_000913 3423789–3424234 3424531–3424239 6–743 6 [5] [5] 6 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)38 (1.110) 32/256 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =62 (1.760)83 (2.370) 44/262 0.0 73.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)33 (0.940) 29/264 0.2 97.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA