breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 5,439 A→C 17.7% H69P (CAC→CCC)  Y75_RS00030 → DUF2502 domain‑containing protein
RA 8,698 A→G 100% N154S (AAC→AGC)  tal → transaldolase
RA 8,972 A→G 100% A245A (GCA→GCG tal → transaldolase
RA 19,427 A→G 30.0% N236S (AAT→AGT)  nhaR → transcriptional activator NhaR
RA 19,523 A→G 100% Y268C (TAC→TGC)  nhaR → transcriptional activator NhaR
RA 21,017 T→C 6.6% N21S (AAC→AGC)  rpsT ← 30S ribosomal protein S20
RA 56,379 T→G 12.1% Y244S (TAC→TCC)  lptD ← LPS assembly protein LptD
RA 73,407 T→C 11.3% E372G (GAG→GGG)  thiP ← thiamine/thiamine pyrophosphate ABC transporter permease ThiP
RA 75,079 C→T 20.1% L134L (CTG→CTA thiB ← thiamine ABC transporter substrate binding subunit
RA 82,766 A→G 7.6% L255P (CTC→CCC)  leuA ← 2‑isopropylmalate synthase
RA 119,148 C→G 9.6% A139G (GCA→GGA)  ampD → 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD
RA 119,388 A→G 8.7% K36K (AAA→AAG ampE → beta‑lactamase regulator AmpE
RA 152,161 A→G 40.2% V24A (GTA→GCA)  yadL ← fimbrial‑like protein YadL
RA 160,542 T→C 100% E21E (GAA→GAG Y75_RS00735 ← RNA polymerase‑binding protein DksA
RA 176,773 T→G 7.7% V55G (GTG→GGG)  erpA → iron‑sulfur cluster insertion protein ErpA
RA 179,350 T→C 100% R38R (CGT→CGC dgt → dGTPase
RA 228,688 Δ1 bp 100% intergenic (+9/‑70) Y75_RS01035 → / → rrf 23S ribosomal RNA/5S ribosomal RNA
RA 245,321 A→G 100% L162P (CTG→CCG)  Y75_RS01145 ← murein L,D‑transpeptidase
RA 265,219 A→C 13.7% G31G (GGT→GGG Y75_RS01275 ← hypothetical protein
RA 265,233 A→C 14.6% F27V (TTC→GTC)  Y75_RS01275 ← hypothetical protein
RA 267,754 T→C 100% Y144C (TAC→TGC)  Y75_RS01295 ← GTPase family protein
RA 275,552 T→C 100% V335A (GTG→GCG)  mmuP → S‑methylmethionine permease
RA 300,601 A→C 8.5% L171V (TTG→GTG)  Y75_RS01475 ← aldehyde oxidoreductase FAD‑binding subunit PaoB
RA 332,215 G→T 14.2% L207F (TTG→TTT Y75_RS01630 → DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
RA 344,526 C→A 16.4% intergenic (+311/‑102) Y75_RS01685 → / → Y75_RS01690 hypothetical protein/hypothetical protein
RA 352,830 A→G 15.2% M301V (ATG→GTG)  prpE → propionate‑‑CoA ligase
RA 359,586 T→G 19.1% V133G (GTG→GGG)  cynX → cyanate transporter CynX
RA 359,594 C→G 7.5% L136V (CTG→GTG)  cynX → cyanate transporter CynX
RA 363,258 G→T 6.2% Q758K (CAA→AAA)  Y75_RS01770 ← beta‑galactosidase
RA 365,732 G→T 14.6% Q335K (CAA→AAA)  Y75_RS01775 ← DNA‑binding transcriptional repressor LacI
RA 374,269 G→A 5.2% intergenic (+164/‑414) dmpG → / → Y75_RS01815 4‑hydroxy‑2‑oxovalerate aldolase/3‑(3‑hydroxy‑phenyl)propionate transporter
RA 392,802 A→G 6.9% pseudogene (603/1443 nt) Y75_RS01915 → autotransporter outer membrane beta‑barrel domain‑containing protein
RA 422,258 A→G 100% T174A (ACG→GCG)  malZ → maltodextrin glucosidase
RA 432,873 T→G 33.8% G65G (GGT→GGG ribD → bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase RibD
RA 446,901 G→T 11.0% T10K (ACG→AAG)  cyoE ← protoheme IX farnesyltransferase
RA 474,969 A→G 23.3% T123A (ACC→GCC)  Y75_RS02330 → hypothetical protein
RA 497,850 T→C 10.8% L191P (CTC→CCC)  hemH → ferrochelatase
RA 501,700 A→G 100% F255L (TTC→CTC)  Y75_RS02460 ← Kef family K(+) transporter
RA 537,594 C→G 12.3% S246R (AGC→AGG Y75_RS02615 → putative allantoin permease
RA 537,607 A→T 11.5% T251S (ACG→TCG)  Y75_RS02615 → putative allantoin permease
RA 547,694 A→G 100% E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,831:1 +G 100% coding (251/261 nt) Y75_RS02655 → hypothetical protein
RA 550,454 A→G 12.6% S265G (AGT→GGT)  Y75_RS02670 → carbamate kinase
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
RA 595,716 C→G 9.8% G298G (GGC→GGG cusC → Cu(+)/Ag(+) efflux RND transporter outer membrane channel CusC
RA 595,722 A→C 12.2% A300A (GCA→GCC cusC → Cu(+)/Ag(+) efflux RND transporter outer membrane channel CusC
RA 601,858 A→G 15.1% E226G (GAG→GGG)  pheP → phenylalanine transporter
RA 616,788 T→C 45.0% Y1137H (TAT→CAT)  entF → enterobactin non‑ribosomal peptide synthetase EntF
RA 627,875 T→G 8.8% G34G (GGT→GGG dhbA → 2,3‑dihydro‑2,3‑dihydroxybenzoate dehydrogenase
RA 629,410 A→G 10.0% G98G (GGA→GGG cstA → carbon starvation protein CstA
RA 645,964 C→T 6.7% A257T (GCC→ACC)  citG ← triphosphoribosyl‑dephospho‑CoA synthase CitG
RA 647,873 T→C 100% T308A (ACC→GCC)  citF ← citrate lyase subunit alpha
RA 649,967 A→G 100% S14P (TCT→CCT)  citD ← citrate lyase acyl carrier protein
RA 658,072 C→A 13.4% G97C (GGC→TGC)  crcB ← fluoride efflux transporter CrcB
RA 658,098 A→C 21.6% V88G (GTG→GGG)  crcB ← fluoride efflux transporter CrcB
RA 663,570 A→G 100% R272R (CGT→CGC dacA ← D‑alanyl‑D‑alanine carboxypeptidase DacA
RA 693,319 T→C 10.7% E174G (GAG→GGG)  Y75_RS03425 ← PhoH family protein
RA 717,946 T→C 13.3% I248V (ATT→GTT)  potE ← putrescine‑ornithine antiporter
RA 718,961 T→C 100% E641G (GAA→GGA)  speF ← ornithine decarboxylase SpeF
RA 728,912 C→A 12.3% L81F (TTG→TTT kdpA ← potassium‑transporting ATPase subunit KdpA
RA 762,477 T→C 11.4% R178R (CGT→CGC odhB → 2‑oxoglutarate dehydrogenase complex dihydrolipoyllysine‑residue succinyltransferase
RA 773,431 A→G 28.0% T518A (ACT→GCT)  cydA → cytochrome ubiquinol oxidase subunit I
RA 783,159 A→G 13.8% Y218C (TAC→TGC)  nadA → quinolinate synthase NadA
RA 783,715 A→G 8.4% E43G (GAG→GGG)  Y75_RS03920 → nicotinamide riboside transporter PnuC
RA 798,640 A→G 100% Y72H (TAT→CAT)  Y75_RS04000 ← bifunctional pyridoxal phosphate/fructose‑1,6‑bisphosphate phosphatase
RA 812,143 T→G 23.7% V67G (GTG→GGG)  bioC → malonyl‑ACP O‑methyltransferase BioC
RA 821,688 A→G 100% E158E (GAA→GAG Y75_RS04105 → Bax inhibitor‑1/YccA family protein
RA 837,003 Δ1 bp 100% coding (231/1086 nt) Y75_RS04175 → hydroxycarboxylate dehydrogenase HcXB
RA 838,200 C→T 100% A50T (GCC→ACC)  Y75_RS04180 ← DUF1471 domain‑containing protein
RA 910,815 T→C 7.7% L219L (TTA→TTG poxB ← ubiquinone‑dependent pyruvate dehydrogenase
RA 912,676 T→C 100% T521A (ACA→GCA)  hcp ← hydroxylamine reductase
RA 944,418 A→G 24.4% T195A (ACC→GCC)  dmsB → dimethylsulfoxide reductase subunit B
RA 948,653 A→G 100% T335A (ACC→GCC)  Y75_RS04680 → amino acid permease
RA 954,782 T→A 13.8% Y36F (TAT→TTT)  focA ← formate transporter FocA
RA 954,796 C→G 22.5% P31P (CCG→CCC focA ← formate transporter FocA
RA 959,499 A→G 13.6% E89G (GAG→GGG)  aroA → 3‑phosphoshikimate 1‑carboxyvinyltransferase
RA 965,122 A→G 5.4% Q127Q (CAA→CAG Y75_RS04750 → ComEC family protein
RA 970,634 C→T 100% A275V (GCG→GTG)  ycaQ → winged helix DNA‑binding protein YcaQ
RA 975,783 A→G 8.9% Y348C (TAC→TGC)  mukF → chromosome partition protein MukF
RA 981,675 T→G 16.4% G69G (GGT→GGG ldtD → L,D‑transpeptidase
RA 986,789 C→T 8.4% G206S (GGC→AGC)  ompF ← porin OmpF
RA 990,365 T→G 26.1% S138S (TCA→TCC pncB ← nicotinate phosphoribosyltransferase
RA 990,394 T→A 12.3% I129F (ATC→TTC)  pncB ← nicotinate phosphoribosyltransferase
RA 990,816 A→G 100% intergenic (‑38/‑228) pncB ← / → pepN nicotinate phosphoribosyltransferase/aminopeptidase N
RA 1,005,273 T→C 27.0% R28R (CGT→CGC pyrD → quinone‑dependent dihydroorotate dehydrogenase
RA 1,009,414 T→C 9.3% T383T (ACT→ACC rlmKL → bifunctional 23S rRNA (guanine(2069)‑N(7))‑methyltransferase RlmK/23S rRNA (guanine(2445)‑N(2))‑methyltransferase RlmL
RA 1,026,432 C→T 100% R514W (CGG→TGG)  helD → DNA helicase IV
RA 1,040,392 T→G 14.9% G225G (GGT→GGG appB → cytochrome d ubiquinol oxidase subunit II
RA 1,058,908 T→C 13.4% S135P (TCG→CCG)  torC → pentaheme c‑type cytochrome TorC
RA 1,065,700 C→T 100% R415C (CGT→TGT)  Y75_RS05220 → YccE family protein
RA 1,079,081 T→G 11.1% H75P (CAC→CCC)  putA ← trifunctional transcriptional regulator/proline dehydrogenase/L‑glutamate gamma‑semialdehyde dehydrogenase
RA 1,080,315 G→T 7.4% A197S (GCC→TCC)  putP → sodium/proline symporter PutP
RA 1,093,686 T→C 100% V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,126,189 C→T 100% A239T (GCT→ACT)  mdtH ← multidrug efflux MFS transporter MdtH
RA 1,163,018 T→G 7.2% A37A (GCA→GCC fhuE ← ferric‑rhodotorulic acid/ferric‑coprogen receptor FhuE
RA 1,174,783 T→G 11.3% L253F (TTA→TTC mfd ← transcription‑repair coupling factor
RA 1,185,092 C→A 8.1% G36C (GGC→TGC)  potC ← spermidine/putrescine ABC transporter permease PotC
RA 1,216,504 T→C 14.9% L183L (TTA→TTG bluF ← blue light‑responsive regulator BluF
RA 1,219,107 A→G 16.1% N68S (AAC→AGC)  Y75_RS06095 → cyclic diguanylate phosphodiesterase
RA 1,234,093 T→A 9.0% D172V (GAT→GTT)  dsbB ← disulfide bond formation protein DsbB
RA 1,260,091:1 +T 100% intergenic (‑1/‑277) pth ← / → ychH aminoacyl‑tRNA hydrolase/stress‑induced protein YchH
RA 1,260,573 T→G 13.8% V69G (GTG→GGG)  ychH → stress‑induced protein YchH
RA 1,270,808 T→C 7.3% A15A (GCA→GCG Y75_RS06360 ← small toxic polypeptide LdrA/LdrC
RA 1,270,827 A→G 5.7% I9T (ATT→ACT)  Y75_RS06360 ← small toxic polypeptide LdrA/LdrC
RA 1,271,028 G→T 5.1% intergenic (‑176/+252) Y75_RS06360 ← / ← Y75_RS06365 small toxic polypeptide LdrA/LdrC/small toxic polypeptide LdrB
RA 1,271,051 A→T 5.8% intergenic (‑199/+229) Y75_RS06360 ← / ← Y75_RS06365 small toxic polypeptide LdrA/LdrC/small toxic polypeptide LdrB
RA 1,273,404 A→T 21.2% V8E (GTA→GAA)  chaA ← sodium‑potassium/proton antiporter ChaA
RA 1,273,462 T→A 8.7% intergenic (‑36/‑234) chaA ← / → chaB sodium‑potassium/proton antiporter ChaA/putative cation transport regulator ChaB
RA 1,284,989 T→C 100% G1183G (GGT→GGC Y75_RS06420 → nitrate reductase subunit alpha
RA 1,303,712 A→G 100% S273G (AGC→GGC)  oppA → oligopeptide ABC transporter substrate‑binding protein OppA
RA 1,329,104 T→C 5.2% I180T (ATC→ACC)  rluB → 23S rRNA pseudouridine(2605) synthase RluB
RA 1,331,342 T→C 7.4% S99S (AGT→AGC sohB → protease SohB
RA 1,333,363 T→C 5.5% V201A (GTT→GCT)  topA → type I DNA topoisomerase
RA 1,349,144 A→G 52.8% W495R (TGG→CGG)  rnb ← exoribonuclease II
RA 1,349,152 T→C 5.2% Y492C (TAC→TGC)  rnb ← exoribonuclease II
RA 1,350,607 A→G 20.4% L7P (CTG→CCG)  rnb ← exoribonuclease II
RA 1,356,817 A→G 100% V123A (GTG→GCG)  sapB ← peptide ABC transporter permease SapB
RA 1,365,413 A→G 100% L319L (TTA→TTG puuC → aldehyde dehydrogenase PuuC
RA 1,379,398 A→C 10.2% T285P (ACC→CCC)  Y75_RS06935 → Gfo/Idh/MocA family oxidoreductase
RA 1,386,502 T→G 8.6% G224G (GGT→GGG Y75_RS06965 → YcjX family protein
RA 1,393,120 T→C 51.6% N150D (AAT→GAT)  Y75_RS07000 ← alpha/beta hydrolase
MC JC 1,413,639 Δ23,060 bp 100% [ttcA]Y75_RS24865 33 genes
RA 1,457,560 A→C 10.6% H228P (CAC→CCC)  paaC → phenylacetate‑CoA oxygenase subunit PaaC
RA 1,457,976 C→A 100% A113D (GCC→GAC)  paaD → phenylacetate degradation protein PaaD
RA 1,464,132 T→C 100% G98G (GGT→GGC paaF → phenylacetate‑‑CoA ligase
RA 1,482,657 C→T 33.7% A20V (GCC→GTC)  Y75_RS07450 → phosphatase PAP2/dual specificity phosphatase family protein
RA 1,531,018 A→C 13.9% H389P (CAC→CCC)  Y75_RS07695 → RHS repeat protein
RA 1,550,225 T→C 100% Y371H (TAC→CAC)  fdnG → formate dehydrogenase‑N subunit alpha
RA 1,553,109 A→G 8.1% T20A (ACC→GCC)  fdnI → formate dehydrogenase‑N subunit gamma
RA 1,555,753 A→G 18.6% L544P (CTG→CCG)  Y75_RS07805 ← malate dehydrogenase
RA 1,565,102 T→G 13.1% L782L (CTA→CTC dosP ← oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
RA 1,568,023 G→T 14.3% Q278K (CAA→AAA)  dosC ← diguanylate cyclase DosC
RA 1,590,915 A→G 100% pseudogene (879/1227 nt) Y75_RS07940 ← fimbrial biogenesis outer membrane usher protein
RA 1,597,551 A→G 13.1% pseudogene (2250/4025 nt) Y75_RS07965 ← autotransporter barrel domain‑containing lipoprotein
RA 1,605,637 T→G 16.8% V302G (GTG→GGG)  lsrC → autoinducer 2 ABC transporter permease LsrC
RA 1,623,852 C→G 30.9% N269K (AAC→AAG ydeE → efflux MFS transporter YdeE
RA 1,628,459 A→G 100% G212G (GGT→GGC dcp ← peptidyl‑dipeptidase Dcp
RA 1,674,232 T→C 5.6% Y187H (TAC→CAC)  Y75_RS08420 → serine protease
RA 1,687,275 T→C 100% N343S (AAC→AGC)  fumC ← class II fumarate hydratase
RA 1,707,042 A→G 100% T27A (ACG→GCG)  Y75_RS08565 → DUF2569 domain‑containing protein
RA 1,707,215 T→C 100% R84R (CGT→CGC Y75_RS08565 → DUF2569 domain‑containing protein
RA 1,712,704 A→G 100% T55A (ACC→GCC)  Y75_RS08595 → electron transport complex subunit RsxE
RA 1,715,402 T→C 13.6% V307A (GTT→GCT)  dtpA → dipeptide/tripeptide permease DtpA
RA 1,715,957 A→G 100% D492G (GAC→GGC)  dtpA → dipeptide/tripeptide permease DtpA
RA 1,733,814 G→A 100% G1005D (GGC→GAC)  Y75_RS08705 → ATP‑dependent helicase
RA 1,739,298 A→G 100% intergenic (‑39/‑260) ynhF ← / → purR stress response protein YnhF/HTH‑type transcriptional repressor PurR
RA 1,743,754 T→C 25.8% C210R (TGT→CGT)  cfa → cyclopropane fatty acyl phospholipid synthase
RA 1,773,659 T→C 7.2% V292A (GTC→GCC)  Y75_RS08900 → MFS transporter
RA 1,774,129 T→C 9.1% intergenic (+130/‑97) Y75_RS08900 → / → Y75_RS08905 MFS transporter/MFS transporter
RA 1,789,889 A→G 100% Y244C (TAC→TGC)  ppsR → posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR
RA 1,795,962 T→C 100% E302G (GAG→GGG)  btuC ← vitamin B12 ABC transporter permease BtuC
RA 1,813,544 G→A 100% V299V (GTG→GTA Y75_RS09105 → cystine/sulfocysteine:cation symporter
RA 1,826,815 T→C 100% intergenic (‑164/+39) ves ← / ← spy environmental stress‑induced protein Ves/ATP‑independent periplasmic protein‑refolding chaperone
RA 1,840,153 G→A 10.8% A410T (GCG→ACG)  Y75_RS09240 → ABC transporter permease subunit
RA 1,848,612 T→C 23.2% K21K (AAA→AAG Y75_RS09280 ← DNA topoisomerase III
RA 1,878,196 A→T 11.6% L270F (TTA→TTT nimT → 2‑nitroimidazole transporter
RA 1,880,274 A→G 11.0% G282G (GGA→GGG dgcP → diguanylate cyclase DgcP
RA 1,881,068:1 +T 100% intergenic (‑99/+49) Y75_RS09455 ← / ← Y75_RS09460 GlsB/YeaQ/YmgE family stress response membrane protein/DUF1869 domain‑containing protein
RA 1,889,098 A→G 8.4% I199T (ATC→ACC)  rnd ← ribonuclease D
RA 1,891,010 G→A 100% R151C (CGT→TGT)  fadD ← long‑chain‑fatty‑acid‑‑CoA ligase FadD
RA 1,892,829 A→G 6.2% T51T (ACT→ACC tsaB ← tRNA (adenosine(37)‑N6)‑threonylcarbamoyltransferase complex dimerization subunit type 1 TsaB
RA 1,895,620 A→G 5.8% intergenic (+195/‑167) Y75_RS09525 → / → Y75_RS09530 RidA family protein/DUF1889 family protein
RA 1,897,648 A→G 100% E377G (GAA→GGA)  pabB → aminodeoxychorismate synthase component 1
RA 1,925,886 T→C 100% E140E (GAA→GAG copD ← copper homeostasis membrane protein CopD
RA 1,977,463 T→C 100% E169E (GAA→GAG Y75_RS09960 ← flagellar motor protein MotB
RA 1,977,759 T→C 100% T71A (ACG→GCG)  Y75_RS09960 ← flagellar motor protein MotB
RA 2,003,875 G→A 9.1% Q18* (CAG→TAG)  Y75_RS10125 ← RNA polymerase sigma factor FliA
RA 2,005,721 T→C 8.2% N8S (AAC→AGC)  fliC ← H48 family flagellin FliC
RA 2,005,956 A→G 100% intergenic (‑213/‑53) fliC ← / → fliD H48 family flagellin FliC/flagellar filament capping protein FliD
RA 2,008,180 G→A 6.4% G111S (GGC→AGC)  fliT → flagella biosynthesis regulatory protein FliT
RA 2,047,421 A→G 100% D116G (GAT→GGT)  yeeJ → inverse autotransporter adhesin YeeJ
RA 2,064,365 A→G 5.1% intergenic (‑295/‑32) nac ← / → Y75_RS10445 nitrogen assimilation transcriptional regulator/tRNA‑Asn
RA 2,065,115 T→C 100% T117A (ACT→GCT)  ldtA ← L,D‑transpeptidase
RA 2,095,845 A→C 10.5% T81P (ACC→CCC)  hisB → bifunctional histidinol‑phosphatase/imidazoleglycerol‑phosphate dehydratase HisB
RA 2,119,805 G→A 100% P246L (CCG→CTG)  wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA 2,123,615 A→G 100% I26T (ATC→ACC)  Y75_RS10745 ← UDP‑glucose‑‑undecaprenyl‑phosphate glucose‑1‑phosphate transferase
RA 2,126,697 T→C 8.3% N396S (AAC→AGC)  wcaI ← colanic acid biosynthesis fucosyltransferase WcaI
RA 2,127,707 T→C 5.6% K59K (AAA→AAG wcaI ← colanic acid biosynthesis fucosyltransferase WcaI
RA 2,144,211 T→C 9.5% T48A (ACG→GCG)  dcd ← dCTP deaminase
RA 2,144,946 T→C 100% D47G (GAC→GGC)  udk ← uridine kinase
RA 2,152,044 A→C 17.4% V360G (GTG→GGG)  yegI ← protein kinase YegI
RA 2,153,722 C→T 23.3% P134L (CCT→CTT)  Y75_RS10870 → YegJ family protein
RA 2,153,826 G→T 28.7% intergenic (+43/+22) Y75_RS10870 → / ← pphC YegJ family protein/protein‑serine/threonine phosphatase PphC
RA 2,153,828 C→T 23.7% intergenic (+45/+20) Y75_RS10870 → / ← pphC YegJ family protein/protein‑serine/threonine phosphatase PphC
RA 2,158,112 T→C 5.3% L238P (CTT→CCT)  mdtB → multidrug efflux RND transporter permease subunit MdtB
RA 2,173,673 A→C 9.8% pseudogene (198/306 nt) Y75_RS10955 ← DeoR/GlpR transcriptional regulator
RA 2,179,979 G→A 100% P187L (CCG→CTG)  gatY ← tagatose‑bisphosphate aldolase subunit GatY
RA 2,188,533 T→G 21.7% T35P (ACC→CCC)  thiM ← hydroxyethylthiazole kinase
RA 2,204,029 T→C 19.2% P139P (CCT→CCC Y75_RS11105 → DUF4132 domain‑containing protein
RA 2,234,532 A→G 100% E60E (GAA→GAG Y75_RS11235 → CidB/LrgB family autolysis modulator
RA 2,235,859 G→A 6.1% A228T (GCT→ACT)  cdd → cytidine deaminase
RA 2,236,204 T→C 100% intergenic (+142/‑8) cdd → / → sanA cytidine deaminase/outer membrane permeability protein SanA
RA 2,249,292 C→T 13.8% R271H (CGC→CAC)  cirA ← catecholate siderophore receptor CirA
RA 2,269,816 A→G 5.1% T80A (ACC→GCC)  Y75_RS11400 → mannitol dehydrogenase family protein
RA 2,271,636 G→A 82.3% T158T (ACG→ACA Y75_RS11405 → zinc‑binding GTPase YeiR
RA 2,274,900 T→C 5.3% Y281H (TAT→CAT)  Y75_RS11420 → cyclic di‑GMP phosphodiesterase
RA 2,274,950 A→G 27.6% V297V (GTA→GTG Y75_RS11420 → cyclic di‑GMP phosphodiesterase
RA 2,293,723 T→C 10.0% intergenic (‑308/‑111) Y75_RS11495 ← / → narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
RA 2,294,824 C→T 11.8% Q307Q (CAG→CAA Y75_RS11510 ← cytochrome c‑type biogenesis thiol:disulfide oxidoreductase CcmH
RA 2,307,983 C→T 7.2% intergenic (+256/+459) eco → / ← mqo serine protease inhibitor ecotin/malate dehydrogenase (quinone)
RA 2,308,240 T→C 8.1% intergenic (+513/+202) eco → / ← mqo serine protease inhibitor ecotin/malate dehydrogenase (quinone)
RA 2,320,623 A→G 100% pseudogene (170/432 nt) Y75_RS11645 ← response regulator
MC JC 2,320,795 Δ1,336 bp 100% Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,324,420 G→A 7.4% pseudogene (127/2421 nt) rcsC ← two‑component system sensor histidine kinase RcsC
RA 2,341,264 A→C 16.8% G17G (GGT→GGG Y75_RS11720 ← DUF2138 domain‑containing protein
RA 2,347,609 C→T 100% G411S (GGC→AGC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA 2,353,303 T→G 11.5% G40G (GGT→GGG yfaE → ferredoxin‑like diferric‑tyrosyl radical cofactor maintenance protein YfaE
RA 2,365,255 T→C 8.5% H277R (CAC→CGC)  rhmD ← L‑rhamnonate dehydratase
RA 2,372,052 G→A 13.2% Q104Q (CAG→CAA arnC → undecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
RA 2,374,075 T→C 17.8% V456A (GTG→GCG)  arnA → bifunctional UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronic acid oxidase ArnA
RA 2,380,056 A→G 5.8% C193R (TGC→CGC)  menC ← o‑succinylbenzoate synthase
RA 2,381,027 T→C 12.3% T155A (ACT→GCT)  menB ← 1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase
RA 2,383,639 T→C 18.0% G97G (GGA→GGG menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase
RA 2,383,659 T→C 11.6% I91V (ATT→GTT)  menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylic‑acid synthase
RA 2,418,020 T→C 15.9% Q7Q (CAA→CAG Y75_RS12060 ← YfbU family protein
RA 2,429,952 A→G 22.3% S2P (TCC→CCC)  hisP ← histidine ABC transporter ATP‑binding protein HisP
RA 2,458,635 T→C 13.0% pseudogene (1813/2646 nt) yfcU ← fimbrial usher protein YfcU
RA 2,464,818 T→C 6.5% A366A (GCA→GCG fadI ← acetyl‑CoA C‑acyltransferase FadI
RA 2,469,945 T→A 11.2% Y170F (TAC→TTC)  mlaA ← phospholipid‑binding lipoprotein MlaA
RA 2,477,843 A→G 26.8% V162A (GTG→GCG)  Y75_RS12375 ← PerC family transcriptional regulator
RA 2,484,772 T→C 100% V42A (GTT→GCT)  dsdA → D‑serine ammonia‑lyase
RA 2,511,805 T→C 8.2% T92A (ACG→GCG)  ypdF ← aminopeptidase
RA 2,513,660 G→A 100% A10V (GCC→GTC)  Y75_RS12535 ← fructose‑like phosphotransferase enzyme IIB component 1
RA 2,530,688 C→A 7.2% T39N (ACC→AAC)  Y75_RS12650 → outer membrane protein
RA 2,535,727 T→C 100% D318G (GAC→GGC)  zipA ← cell division protein ZipA
RA 2,536,366 A→G 8.1% V105A (GTC→GCC)  zipA ← cell division protein ZipA
RA 2,538,886 T→C 50.6% intergenic (+60/‑324) cysK → / → ptsH cysteine synthase A/phosphocarrier protein Hpr
RA 2,545,887 T→C 100% Q125R (CAG→CGG)  cysA ← sulfate/thiosulfate ABC transporter ATP‑binding protein CysA
RA 2,565,892 A→G 100% V291A (GTG→GCG)  eutH ← ethanolamine utilization protein EutH
RA 2,579,295 A→G 8.2% D335G (GAC→GGC)  tkt → transketolase
RA 2,605,808 T→C 24.3% F79S (TTC→TCC)  hyfF → hydrogenase 4 subunit F
RA 2,606,882 T→G 21.0% V437G (GTA→GGA)  hyfF → hydrogenase 4 subunit F
RA 2,612,091 A→G 8.1% P512P (CCA→CCG Y75_RS13040 → DNA‑binding transcriptional regulator HyfR
RA 2,616,247 T→A 8.7% V5E (GTA→GAA)  arsC → arsenate reductase (glutaredoxin)
RA 2,621,296 T→C 100% V136A (GTG→GCG)  purN → phosphoribosylglycinamide formyltransferase
RA 2,621,951 G→A 100% V84V (GTG→GTA ppk1 → polyphosphate kinase 1
RA 2,627,276 T→A 20.8% T107S (ACT→TCT)  pdeF ← cyclic‑guanylate‑specific phosphodiesterase PdeF
RA 2,627,287 T→A 9.5% Y103F (TAC→TTC)  pdeF ← cyclic‑guanylate‑specific phosphodiesterase PdeF
RA 2,634,872 T→C 22.8% T381A (ACC→GCC)  der ← ribosome biogenesis GTPase Der
RA 2,646,727 T→C 77.6% Q1406R (CAG→CGG)  yfhM ← alpha2‑macroglobulin
RA 2,650,563 G→A 6.1% R127R (CGC→CGT yfhM ← alpha2‑macroglobulin
RA 2,666,599 A→G 100% intergenic (‑97/+63) Y75_RS13270 ← / ← Y75_RS13275 3‑phenylpropionate MFS transporter/DNA‑binding transcriptional regulator HcaR
RA 2,668,960 T→C 100% Y425H (TAC→CAC)  Y75_RS13280 → aromatic ring‑hydroxylating dioxygenase subunit alpha
RA 2,675,299 G→A 100% A61A (GCC→GCT Y75_RS13320 ← ABC transporter permease
RA 2,676,395 C→T 10.2% R208Q (CGG→CAG)  Y75_RS13325 ← sugar ABC transporter ATP‑binding protein
RA 2,685,344 G→A 9.5% G285D (GGC→GAC)  hmpA → NO‑inducible flavohemoprotein
RA 2,693,128 G→A 20.5% R358C (CGC→TGC)  purL ← phosphoribosylformylglycinamidine synthase
RA 2,704,068 A→C 10.0% G571G (GGT→GGG lepA ← elongation factor 4
RA 2,704,827 G→A 7.1% S318S (AGC→AGT lepA ← elongation factor 4
RA 2,723,361 G→A 81.2% L348L (CTG→TTG)  kgtP ← alpha‑ketoglutarate permease
RA 2,743,082 A→G 48.5% G35G (GGT→GGC rplS ← 50S ribosomal protein L19
RA 2,747,579 A→G 100% K50K (AAA→AAG Y75_RS13680 → HlyC/CorC family transporter
RA 2,751,156 G→A 8.0% D236N (GAC→AAC)  recN → DNA repair protein RecN
RA 2,764,229 T→C 6.9% E68E (GAA→GAG Y75_RS13765 ← DUF1524 domain‑containing protein
RA 2,768,585 T→C 100% V76A (GTG→GCG)  Y75_RS13790 → WYL domain‑containing protein
RA 2,775,211 A→G 10.1% E57G (GAG→GGG)  Y75_RS13830 → RadC family protein
RA 2,779,100 A→G 100% D761D (GAT→GAC ypjA ← adhesin‑like autotransporter YpjA/EhaD
RA 2,790,905 T→C 100% V326A (GTC→GCC)  gabD → NADP‑dependent succinate‑semialdehyde dehydrogenase
RA 2,795,799 T→C 100% intergenic (‑115/‑68) Y75_RS13915 ← / → Y75_RS13920 YqaE/Pmp3 family membrane protein/helix‑turn‑helix transcriptional regulator
RA 2,818,531 G→A 28.5% R713C (CGC→TGC)  alaS ← alanine‑‑tRNA ligase
RA 2,819,373 T→C 6.9% Q432R (CAG→CGG)  alaS ← alanine‑‑tRNA ligase
RA 2,834,637 G→A 100% A482V (GCG→GTG)  hypF ← carbamoyltransferase HypF
RA 2,851,804 T→C 27.6% L338P (CTG→CCG)  hypD → hydrogenase formation protein HypD
RA 2,856,122 T→A 100% L125* (TTG→TAG)  mutS → DNA mismatch repair protein MutS
RA 2,858,090 T→G 15.2% V781G (GTG→GGG)  mutS → DNA mismatch repair protein MutS
RA 2,858,102 T→G 28.6% V785G (GTT→GGT)  mutS → DNA mismatch repair protein MutS
RA 2,866,109 C→A 100% pseudogene (99/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 2,875,230 A→G 23.2% intergenic (‑245/‑7) cysD ← / → iap sulfate adenylyltransferase subunit CysD/alkaline phosphatase isozyme conversion aminopeptidase
RA 2,875,251 G→T 10.9% L5F (TTG→TTT iap → alkaline phosphatase isozyme conversion aminopeptidase
RA 2,890,186 T→C 46.8% E123E (GAA→GAG cysJ ← NADPH‑dependent assimilatory sulfite reductase flavoprotein subunit
RA 2,890,541 A→G 100% V5A (GTC→GCC)  cysJ ← NADPH‑dependent assimilatory sulfite reductase flavoprotein subunit
RA 2,890,872 G→T 12.1% M1M (ATG→ATT) † queD → 6‑carboxytetrahydropterin synthase QueD
RA 2,906,975 C→G 100% D450H (GAC→CAC)  pyrG ← CTP synthase (glutamine hydrolyzing)
JC 2,910,136 Δ12 bp 100% coding (2161‑2172/2235 nt) Y75_RS14515 ← GTP pyrophosphokinase
RA 2,939,008 A→C 11.6% C298G (TGC→GGC)  rlmM ← 23S rRNA (cytidine(2498)‑2'‑O)‑methyltransferase RlmM
RA 2,941,170 T→C 100% A18A (GCA→GCG Y75_RS14640 ← glycine cleavage system transcriptional regulator GcvA
RA 2,973,749 T→G 9.8% T308P (ACT→CCT)  aas ← bifunctional acyl‑ACP‑‑phospholipid O‑acyltransferase/long‑chain‑fatty‑acid‑‑ACP ligase
RA 2,976,822 T→A 16.2% Y245F (TAT→TTT)  lysA ← diaminopimelate decarboxylase
RA 2,981,321 T→C 100% Q198Q (CAA→CAG kduD ← 2‑dehydro‑3‑deoxy‑D‑gluconate 5‑dehydrogenase KduD
RA 2,999,613 A→G 100% T218A (ACC→GCC)  xdhA → xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 2,999,815 T→C 13.5% L285P (CTC→CCC)  xdhA → xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,033,104 A→G 46.6% Y243H (TAC→CAC)  lysS ← lysine‑‑tRNA ligase
RA 3,038,640 G→A 7.2% E44K (GAG→AAG)  fldB → flavodoxin FldB
RA 3,043,955 T→C 7.0% G201G (GGA→GGG Y75_RS15125 ← SDR family oxidoreductase
RA 3,045,505 T→C 26.9% A731A (GCA→GCG gcvP ← aminomethyl‑transferring glycine dehydrogenase
RA 3,061,374 A→C 6.8% T623T (ACA→ACC scpA → methylmalonyl‑CoA mutase
RA 3,071,463 G→A 100% A295A (GCC→GCT epd ← erythrose‑4‑phosphate dehydrogenase
RA 3,085,013 G→A 100% R57R (CGG→CGA Y75_RS15310 → hypothetical protein
RA 3,104,180 G→A 7.4% intergenic (+12/‑190) mltC → / → Y75_RS15435 membrane‑bound lytic murein transglycosylase MltC/nucleoside permease NupG
RA 3,108,691 T→C 8.1% S161S (AGT→AGC Y75_RS15445 → DUF554 domain‑containing protein
RA 3,146,883 G→A 15.0% G111S (GGT→AGT)  gpr → L‑glyceraldehyde 3‑phosphate reductase
RA 3,152,550 A→C 16.1% H111P (CAC→CCC)  Y75_RS15660 → DedA family protein
RA 3,155,352 C→A 8.6% A25E (GCA→GAA)  dkgA → 2,5‑didehydrogluconate reductase DkgA
RA 3,168,585 T→C 100% R34R (CGT→CGC qseB → two‑component system response regulator QseB
RA 3,178,798 T→C 37.1% R133R (CGT→CGC Y75_RS15800 → DUF1190 family protein
RA 3,194,166 T→C 100% D415G (GAC→GGC)  hldE ← bifunctional D‑glycero‑beta‑D‑manno‑heptose‑7‑phosphate kinase/D‑glycero‑beta‑D‑manno‑heptose 1‑phosphate adenylyltransferase HldE
RA 3,196,264 G→A 7.9% R678R (CGC→CGT glnE ← bifunctional [glutamate‑‑ammonia ligase]‑adenylyl‑L‑tyrosine phosphorylase/[glutamate‑‑ammonia‑ligase] adenylyltransferase
RA 3,202,142 A→G 9.4% W216R (TGG→CGG)  bacA ← undecaprenyl‑diphosphate phosphatase
RA 3,203,757 G→T 11.6% L136F (TTG→TTT plsY → glycerol‑3‑phosphate 1‑O‑acyltransferase PlsY
RA 3,205,094 G→A 23.9% intergenic (‑182/‑25) ttdR ← / → ttdA DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
RA 3,205,108 C→A 21.3% intergenic (‑196/‑11) ttdR ← / → ttdA DNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
RA 3,205,127 C→G 11.9% S3R (AGC→AGG ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,139 G→A 22.2% K7K (AAG→AAA ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,140 C→A 16.6% Q8K (CAA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,205,162 C→A 9.4% T15K (ACA→AAA)  ttdA → L(+)‑tartrate dehydratase subunit alpha
RA 3,222,220 T→C 100% V311A (GTG→GCG)  ebgA → beta‑galactosidase subunit alpha
RA 3,226,690 T→C 100% G78G (GGT→GGC ygjJ → protein YgjJ
RA 3,230,205 T→C 100% intergenic (+310/‑116) ygjK → / → Y75_RS16040 alpha‑glucosidase/NADPH‑dependent 2,4‑dienoyl‑CoA reductase
RA 3,231,977 C→T 5.2% R553C (CGT→TGT)  Y75_RS16040 → NADPH‑dependent 2,4‑dienoyl‑CoA reductase
RA 3,247,046 T→C 100% G206G (GGT→GGC Y75_RS16115 → DedA family protein
RA 3,252,124 G→A 100% V249V (GTC→GTT Y75_RS16165 ← DNA‑binding transcriptional regulator YhaJ
RA 3,258,241 G→T 6.2% P22Q (CCA→CAA)  Y75_RS16190 ← L‑serine ammonia‑lyase
MC JC 3,261,134 Δ1,199 bp 100% Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,277,364 A→G 6.8% E58G (GAG→GGG)  Y75_RS16280 → type II toxin‑antitoxin system ribonuclease toxin YhaV
RA 3,278,946 A→C 13.9% T60P (ACC→CCC)  kbaZ → tagatose‑bisphosphate aldolase subunit KbaZ
RA 3,280,261 C→A 8.2% Q64K (CAA→AAA)  Y75_RS16295 → PTS N‑acetylgalactosamine transporter subunit IIB
RA 3,284,089 T→C 100% V22A (GTG→GCG)  Y75_RS16320 → PTS N‑acetylgalactosamine transporter subunit IIB
RA 3,285,754 A→T 12.2% Y141F (TAC→TTC)  Y75_RS16330 → PTS N‑acetylgalactosamine transporter subunit IID
RA 3,292,756 T→C 8.9% L145L (TTA→TTG rsmI ← 16S rRNA (cytidine(1402)‑2'‑O)‑methyltransferase
RA 3,309,942 T→G 100% D361A (GAC→GCC)  pnp ← polyribonucleotide nucleotidyltransferase
RA 3,316,515 T→G 100% A289A (GCA→GCC nusA ← transcription termination/antitermination protein NusA
RA 3,317,320 T→C 100% E21G (GAG→GGG)  nusA ← transcription termination/antitermination protein NusA
RA 3,324,200 A→C 8.8% G189G (GGT→GGG folP ← dihydropteroate synthase
RA 3,325,735 A→G 8.1% G352G (GGT→GGC ftsH ← ATP‑dependent zinc metalloprotease FtsH
RA 3,335,060 C→T 53.0% intergenic (+18/+30) sfsB → / ← murA DNA‑binding transcriptional regulator SfsB/UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,337,200 T→C 100% T183A (ACG→GCG)  mlaC ← phospholipid‑binding protein MlaC
RA 3,340,133 C→T 9.0% L2F (CTT→TTT)  Y75_RS16615 → calcium/sodium antiporter
RA 3,370,730 A→C 17.7% G54G (GGT→GGG Y75_RS16755 ← N‑acetylmannosamine‑6‑phosphate 2‑epimerase
RA 3,390,080 A→G 15.7% I236V (ATC→GTC)  aaeR → HTH‑type transcriptional activator AaeR
RA 3,397,873 C→T 100% V121I (GTC→ATC)  yhdE ← nucleoside triphosphate pyrophosphatase YhdE
RA 3,442,749 A→G 100% Q91Q (CAA→CAG thiE → thiamine phosphate synthase
RA 3,455,034 T→C 100% T135A (ACC→GCC)  rpoB ← DNA‑directed RNA polymerase subunit beta
RA 3,474,155 C→A 5.9% Q249K (CAA→AAA)  trmA → tRNA (uridine(54)‑C5)‑methyltransferase TrmA
RA 3,475,831 A→G 100% intergenic (‑271/‑60) fabR ← / → sthA HTH‑type transcriptional repressor FabR/Si‑specific NAD(P)(+) transhydrogenase
RA 3,485,349 C→G 15.5% G589G (GGC→GGG ppc → phosphoenolpyruvate carboxylase
RA 3,486,908 C→T 6.3% L165L (CTG→TTG)  cptA → phosphoethanolamine transferase CptA
RA 3,498,598 T→C 100% V318A (GTC→GCC)  gldA → bifunctional L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA 3,519,498 A→G 18.0% T14A (ACC→GCC)  glpK → glycerol kinase GlpK
RA 3,521,495 A→C 10.3% T132P (ACC→CCC)  glpX → class II fructose‑bisphosphatase
RA 3,541,864 A→G 9.4% E389G (GAA→GGA)  rhaA → L‑rhamnose isomerase
RA 3,571,110 A→T 13.6% T66S (ACC→TCC)  Y75_RS17735 → MFS transporter
RA 3,604,149 A→G 51.9% V14A (GTT→GCT)  Y75_RS17890 ← IMPACT family protein
RA 3,609,529 A→T 8.0% I209N (ATT→AAT)  fre ← NAD(P)H‑flavin reductase
RA 3,609,535 G→C 11.6% A207G (GCG→GGG)  fre ← NAD(P)H‑flavin reductase
RA 3,620,605 Δ1 bp 100% coding (94/816 nt) Y75_RS17970 → dienelactone hydrolase family protein
RA 3,632,590 A→T 10.9% I30F (ATT→TTT)  rarD → EamA family transporter RarD
RA 3,645,110 A→T 100% L140Q (CTG→CAG)  cyaA ← class I adenylate cyclase
RA 3,651,720 G→A 10.1% T349T (ACG→ACA Y75_RS18115 → arylsulfatase AslA
RA 3,656,455 C→T 100% V91I (GTT→ATT)  rffM ← lipopolysaccharide N‑acetylmannosaminouronosyltransferase
RA 3,678,574 G→A 15.0% G46G (GGC→GGT ilvC ← ketol‑acid reductoisomerase
RA 3,682,574 C→T 78.6% A210A (GCG→GCA ilvD ← dihydroxy‑acid dehydratase
RA 3,692,566 T→C 100% noncoding (421/2925 nt) Y75_RS18325 ← 23S ribosomal RNA
RA 3,709,331 G→A 27.4% Q66* (CAG→TAG)  asnA ← aspartate‑‑ammonia ligase
RA 3,710,290 T→C 100% L22P (CTG→CCG)  mioC → FMN‑binding protein MioC
RA 3,711,764 G→A 13.2% Q239Q (CAG→CAA mnmG → tRNA uridine(34) 5‑carboxymethylaminomethyl synthesis enzyme MnmG
RA 3,712,414 G→C 100% G456A (GGC→GCC)  mnmG → tRNA uridine(34) 5‑carboxymethylaminomethyl synthesis enzyme MnmG
RA 3,715,056 C→T 100% P143S (CCG→TCG)  atpB → F0F1 ATP synthase subunit A
RA 3,741,162 A→T 9.2% Y364F (TAC→TTC)  Y75_RS18545 → adenine permease AdeP
MC JC 3,742,936 Δ1,336 bp 100% Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
RA 3,746,364 T→G 7.6% L13F (TTA→TTC mdtL ← multidrug efflux MFS transporter MdtL
RA 3,746,729 A→G 5.3% pseudogene (180/375 nt) Y75_RS18580 ← tryptophan permease
MC JC 3,746,911 Δ1,199 bp 100% Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,750,591 Δ1,199 bp 100% Y75_RS18600 Y75_RS18600
RA 3,757,172 G→A 9.7% G163S (GGT→AGT)  dnaA → chromosomal replication initiator protein DnaA
RA 3,764,879 G→T 8.7% T33T (ACC→ACA Y75_RS18670 ← hypothetical protein
RA 3,766,807 A→G 9.1% A289A (GCA→GCG
Q2R (CAG→CGG) 
Y75_RS18680 →
Y75_RS18685 →
2‑dehydro‑3‑deoxygalactonokinase
2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA 3,777,745 A→T 24.0% I312F (ATT→TTT)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,777,754 A→C 27.0% T315P (ACC→CCC)  Y75_RS18735 → PTS transporter subunit EIIC
RA 3,778,980 C→T 26.9% pseudogene (553/815 nt) Y75_RS18745 → inactive 6‑phospho‑alpha‑glucosidase
RA 3,784,364 A→G 100% D86G (GAC→GGC)  Y75_RS18770 → YidH family protein
RA 3,810,059 A→T 9.9% intergenic (‑101/+20) Y75_RS18905 ← / ← xanP AsmA family protein/xanthine/proton symporter XanP
RA 3,819,328 C→A 7.8% A28A (GCC→GCA ligB → NAD‑dependent DNA ligase LigB
RA 3,824,607 Δ1 bp 100% intergenic (+55/‑40) rph → / → pyrE ribonuclease PH/orotate phosphoribosyltransferase
RA 3,855,515 T→G 9.1% V39G (GTG→GGG)  Y75_RS19125 → rhodanese‑like domain‑containing protein
RA 3,857,496 A→G 26.1% D248G (GAT→GGT)  gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,857,502 T→G 16.9% V250G (GTG→GGG)  gpsA → NAD(P)H‑dependent glycerol‑3‑phosphate dehydrogenase
RA 3,858,404 T→G 16.1% G184G (GGT→GGG cysE → serine O‑acetyltransferase
RA 3,858,449 T→G 17.0% G199G (GGT→GGG cysE → serine O‑acetyltransferase
RA 3,858,503 C→G 11.6% G217G (GGC→GGG cysE → serine O‑acetyltransferase
RA 3,858,538 C→G 16.4% P229R (CCG→CGG)  cysE → serine O‑acetyltransferase
RA 3,885,052 T→G 11.8% G383G (GGT→GGG Y75_RS19250 → aldehyde dehydrogenase AldB
RA 3,892,539 T→C 6.1% T265A (ACC→GCC)  lyxK ← L‑xylulokinase
RA 3,908,726 G→T 9.2% Q187K (CAA→AAA)  Y75_RS19370 ← D‑xylose ABC transporter substrate‑binding protein
RA 3,914,027 C→A 7.5% E168* (GAA→TAA)  Y75_RS19395 ← O‑acetyltransferase WecH
RA 3,942,761 T→C 100% V399V (GTA→GTG bcsE ← cellulose biosynthesis protein BcsE
RA 3,950,257 A→G 100% Y35C (TAC→TGC)  bcsZ → cellulase
RA 3,969,883 T→C 100% Y314C (TAC→TGC)  treF ← alpha,alpha‑trehalase TreF
RA 3,984,411 A→G 100% T97A (ACC→GCC)  hdeB → acid‑activated periplasmic chaperone HdeB
RA 3,994,332 A→C 7.1% R233R (CGT→CGG Y75_RS19765 ← 23S rRNA (adenine(2030)‑N(6))‑methyltransferase
RA 3,999,427 T→A 17.0% K43* (AAA→TAA)  dtpB ← dipeptide/tripeptide permease DtpB
RA 4,016,621 A→G 11.4% intergenic (‑78/+12) Y75_RS19850 ← / ← yhhH hypothetical protein/protein YhhH
RA 4,021,098 T→C 14.8% G42G (GGA→GGG Y75_RS19860 ← rhs element protein RhsB
RA 4,023,842 C→T 13.3% G131D (GGC→GAC)  nikC ← nickel ABC transporter permease subunit NikC
RA 4,024,550 T→G 14.9% T209P (ACC→CCC)  nikB ← nickel ABC transporter permease subunit NikB
RA 4,045,020 A→G 16.7% E29G (GAG→GGG)  Y75_RS19995 → branched chain amino acid ABC transporter permease LivM
RA 4,064,228 T→C 100% V292A (GTG→GCG)  gntU → gluconate transporter
RA 4,085,943 A→G 7.9% G463G (GGT→GGC malT ← HTH‑type transcriptional regulator MalT
RA 4,086,357 A→G 5.9% G325G (GGT→GGC malT ← HTH‑type transcriptional regulator MalT
RA 4,099,913 A→G 100% W33R (TGG→CGG)  feoB ← Fe(2+) transporter permease subunit FeoB
RA 4,119,620 C→A 8.7% T75K (ACA→AAA)  hofP → DNA utilization protein HofP
RA 4,123,731 C→T 6.0% P208S (CCA→TCA)  damX → cell division protein DamX
RA 4,129,705 C→G 13.8% A89G (GCG→GGG)  Y75_RS20365 → YhfX family PLP‑dependent enzyme
RA 4,134,515 A→T 9.0% L40F (TTA→TTT Y75_RS20390 → aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
RA 4,140,765 C→A 15.1% intergenic (‑208/+36) frlA ← / ← Y75_RS20420 fructoselysine/psicoselysine transporter/YhfL family protein
RA 4,140,794 C→A 10.3% intergenic (‑237/+7) frlA ← / ← Y75_RS20420 fructoselysine/psicoselysine transporter/YhfL family protein
RA 4,140,812 T→G 7.6% T53P (ACT→CCT)  Y75_RS20420 ← YhfL family protein
RA 4,166,134 C→T 100% P91S (CCG→TCG)  rpsL → 30S ribosomal protein S12
RA 4,179,639 T→G 16.8% T321P (ACC→CCC)  gspF ← type II secretion system inner membrane protein GspF
RA 4,179,667 A→T 10.3% G311G (GGT→GGA gspF ← type II secretion system inner membrane protein GspF
RA 4,198,857 C→T 5.4% G39G (GGC→GGT rpsD → 30S ribosomal protein S4
RA 4,231,754 A→G 13.3% E145G (GAG→GGG)  Y75_RS20955 → Na/Pi cotransporter family protein
RA 4,245,517 T→A 7.3% T110S (ACC→TCC)  Y75_RS21020 ← D‑xylose transporter XylE
RA 4,249,861 C→T 100% G50D (GGT→GAT)  malE ← maltose/maltodextrin ABC transporter substrate‑binding protein MalE
RA 4,262,675 T→C 100% R448R (CGT→CGC dinF → MATE family efflux transporter DinF
RA 4,264,473 T→C 100% V95V (GTT→GTC Y75_RS21115 → DUF2713 domain‑containing protein
RA 4,273,205 T→G 19.2% F68V (TTT→GTT)  Y75_RS21155 → acid phosphatase AphA
RA 4,278,433 T→G 12.1% T67P (ACC→CCC)  Y75_RS21180 ← membrane protein
RA 4,306,137 T→G 13.9% T141P (ACC→CCC)  mdtO ← multidrug efflux transporter permease subunit MdtO
RA 4,311,992 G→C 5.2% pseudogene (486/921 nt) alsK ← allose kinase
RA 4,318,372 A→G 100% E115E (GAA→GAG rpiB → bifunctional allose‑6‑phosphate isomerase/ribose‑5‑phosphate isomerase RpiB
RA 4,322,712 A→G 8.9% L244P (CTG→CCG)  phnK ← phosphonate C‑P lyase system protein PhnK
RA 4,330,626 A→G 6.0% intergenic (‑207/+451) yjdN ← / ← Y75_RS21430 VOC family metalloprotein YjdN/phnA family protein
RA 4,331,214 T→C 100% K67E (AAA→GAA)  Y75_RS21430 ← phnA family protein
RA 4,341,538 T→C 11.0% M58V (ATG→GTG)  adiC ← arginine/agmatine antiporter
RA 4,341,565 T→C 12.6% T49A (ACG→GCG)  adiC ← arginine/agmatine antiporter
RA 4,341,575 T→C 17.9% G45G (GGA→GGG adiC ← arginine/agmatine antiporter
RA 4,341,589 T→C 17.0% I41V (ATT→GTT)  adiC ← arginine/agmatine antiporter
RA 4,347,208 A→C 9.9% Y207S (TAC→TCC)  melA → alpha‑galactosidase
RA 4,348,548 A→T 8.9% N164Y (AAT→TAT)  melB → melibiose:sodium transporter MelB
RA 4,371,271 Δ2 bp 100% pseudogene (182‑183/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
RA 4,408,585 T→G 11.2% G202G (GGT→GGG hflC → protease modulator HflC
RA 4,419,600 T→G 9.7% F216V (TTT→GTT)  Y75_RS21855 → isovaleryl‑CoA dehydrogenase
RA 4,434,652 A→C 15.7% I37L (ATT→CTT)  cycA → D‑serine/D‑alanine/glycine transporter
RA 4,436,515 A→C 11.9% V50G (GTC→GGC)  ytfE ← iron‑sulfur cluster repair protein YtfE
RA 4,442,880 T→C 31.7% V165A (GTG→GCG)  Y75_RS22000 → hypothetical protein
RA 4,443,654 T→C 100% T97A (ACC→GCC)  Y75_RS22005 ← YtfJ family protein
RA 4,455,503 A→G 100% T288A (ACC→GCC)  ytfQ → galactofuranose ABC transporter substrate‑binding protein YtfQ
RA 4,463,104 A→C 14.8% T156P (ACC→CCC)  pmbA → metalloprotease PmbA
RA 4,463,791 A→G 24.1% T385A (ACC→GCC)  pmbA → metalloprotease PmbA
RA 4,469,539 A→C 7.5% V441G (GTG→GGG)  treB ← PTS trehalose transporter subunit IIBC
RA 4,547,544 T→C 100% intergenic (+231/‑251) fimE → / → fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
RA 4,562,036 C→G 10.0% R122G (CGC→GGC)  iraD → anti‑adapter protein IraD
RA 4,562,052 A→G 10.1% E127G (GAG→GGG)  iraD → anti‑adapter protein IraD
RA 4,573,193 G→A 7.4% R3C (CGT→TGT)  mdtM ← multidrug efflux MFS transporter MdtM
RA 4,573,240 C→A 8.7% intergenic (‑41/‑438) mdtM ← / → Y75_RS25100 multidrug efflux MFS transporter MdtM/Rpn family recombination‑promoting nuclease/putative transposase
RA 4,573,245 T→A 7.6% intergenic (‑46/‑433) mdtM ← / → Y75_RS25100 multidrug efflux MFS transporter MdtM/Rpn family recombination‑promoting nuclease/putative transposase
RA 4,574,132 A→G 100% pseudogene (455/921 nt) Y75_RS25100 → Rpn family recombination‑promoting nuclease/putative transposase
RA 4,583,124 T→C 9.8% E298E (GAA→GAG Y75_RS22715 ← 5‑methylcytosine‑specific restriction endonuclease subunit McrB
RA 4,584,336 A→C 13.6% V62G (GTG→GGG)  symE ← endoribonuclease SymE
RA 4,592,222 A→G 7.3% G198G (GGA→GGG Y75_RS22740 → adenine/cytosine‑specific restriction endonuclease Mrr
RA 4,594,941 A→G 6.4% V340A (GTA→GCA)  btsT ← pyruvate/proton symporter BtsT
MC JC 4,631,897 Δ461 bp 100% [radA][nadR] [radA], [nadR]
RA 4,635,020 A→C 11.0% G61G (GGT→GGG ettA ← energy‑dependent translational throttle protein EttA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1301041–1302370 1302407 38–1367 172 [0] [0] 173 Y75_RS06530–Y75_RS06530 Y75_RS06530,Y75_RS06530
* * ÷ NC_007779 1397912–1398957 1406267 7311–8356 65 [63] [0] 140 [Y75_RS07025]–[Y75_RS07065] [Y75_RS07025],Y75_RS07030,uspE,Y75_RS07040,ogt,abgT,Y75_RS07060,[Y75_RS07065]
* * ÷ NC_007779 3986216 3988225–3987179 964–2010 273 [0] [61] 67 [slp]–[Y75_RS19725] [slp],Y75_RS19720,[Y75_RS19725]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 273180 =NA (NA)161 (0.960) 98/432 NT 100% intergenic (+3/+145) Y75_RS01330/Y75_RS01335 hypothetical protein/IS5‑like element IS5 family transposase
?NC_007779 701988 = 0 (0.000)coding (29/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 = 381377NA (NA)6 (0.040) 4/422 NT NA coding (488/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
?NC_007779 = 381470 NA (NA)coding (581/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 = 575008NA (NA)12 (0.070) 10/434 NT 5.7% coding (1041/1068 nt) Y75_RS02850 porin
?NC_007779 579322 = 197 (1.170)intergenic (+13/+152) Y75_RS02880/Y75_RS02885 hypothetical protein/YlcI/YnfO family protein
* ? NC_007779 = 6542130 (0.000)178 (1.090) 95/424 NT 100% pseudogene (1/408 nt) dcuC anaerobic C4‑dicarboxylate transporter DcuC
?NC_007779 655413 = 0 (0.000)pseudogene (978/981 nt) dcuC anaerobic C4‑dicarboxylate transporter DcuC
* ? NC_007779 = 689467NA (NA)160 (0.950) 88/434 NT 100% intergenic (‑32/+298) Y75_RS03405/lnt IS5‑like element IS5 family transposase/apolipoprotein N‑acyltransferase
?NC_007779 = 701992 0 (0.000)coding (25/1221 nt) nagC DNA‑binding transcriptional regulator NagC
* ? NC_007779 1106373 =NA (NA)10 (0.060) 5/412 NT 51.3% coding (174/914 nt) Y75_RS05455 IS3‑like element IS2 family transposase
?NC_007779 1106739 = 10 (0.060)coding (168/361 nt) Y75_RS05455 IS3‑like element IS2 family transposase
* ? NC_007779 1209367 =83 (0.490)114 (0.730) 78/402 NT 61.6% coding (290/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 1211196 = 65 (0.420)intergenic (‑6/‑66) Y75_RS06035/pinE tail fiber protein/site‑specific DNA recombinase
* ? NC_007779 = 120938283 (0.490)71 (0.460) 50/402 NT 50.0% coding (305/630 nt) Y75_RS06020 hypothetical protein
?NC_007779 = 1211179 65 (0.420)coding (12/495 nt) Y75_RS06035 tail fiber protein
* ? NC_007779 1294773 =230 (1.370)26 (0.160) 25/434 NT 10.2% intergenic (‑274/‑331) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 2176983 = NA (NA)intergenic (‑3/‑32) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 = 1294776234 (1.400)24 (0.140) 20/434 NT 9.3% intergenic (‑277/‑328) hns/tdk DNA‑binding transcriptional regulator H‑NS/thymidine kinase
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 = 13010400 (0.000)137 (0.940)
+28 bp
80/378 NT 100% intergenic (+218/‑45) ychE/Y75_RS06530 NAAT family transporter YchE/IS3‑like element IS2 family transposase
?NC_007779 = 4311564 NA (NA)pseudogene (914/921 nt) alsK allose kinase
* ? NC_007779 1302408 =0 (0.000)163 (0.970) 87/434 NT 100% intergenic (+50/‑488) Y75_RS06530/oppA IS3‑like element IS2 family transposase/oligopeptide ABC transporter substrate‑binding protein OppA
?NC_007779 1397758 = NA (NA)intergenic (‑122/‑32) ynaI/Y75_RS07025 low conductance mechanosensitive channel YnaI/IS5‑like element IS5 family transposase
* ? NC_007779 1406268 =0 (0.000)135 (0.810) 78/434 NT 100% coding (12/1311 nt) Y75_RS07065 p‑aminobenzoyl‑glutamate hydrolase subunit AbgA
?NC_007779 1980209 = NA (NA)intergenic (‑298/+146) flhD/Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
* ? NC_007779 = 21769820 (0.000)193 (1.180) 94/424 NT 100% intergenic (‑2/‑33) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
?NC_007779 2178182 = 0 (0.000)pseudogene (202/207 nt) gatA PTS galactitol transporter subunit IIA
* ? NC_007779 = 238050693 (0.550)9 (0.050) 9/428 NT 8.9% coding (127/963 nt) menC o‑succinylbenzoate synthase
?NC_007779 2519723 = NA (NA)intergenic (+33/‑54) nupC/Y75_RS12570 nucleoside permease NupC/IS4‑like element IS421 family transposase
* ? NC_007779 3024216 =150 (0.890)9 (0.050) 9/434 NT 5.7% coding (1210/1401 nt) xanQ xanthine/proton symporter XanQ
?NC_007779 = 3129996 NA (NA)intergenic (+146/+1) Y75_RS15530/Y75_RS15535 IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein
* ? NC_007779 3592355 =1 (0.010)147 (0.880) 78/434 NT 99.3% coding (128/1431 nt) Y75_RS17825 YdgA family protein
?NC_007779 3746911 = NA (NA)intergenic (‑3/‑32) Y75_RS18580/Y75_RS18585 tryptophan permease/IS5‑like element IS5 family transposase
* ? NC_007779 = 35923581 (0.010)174 (1.040) 101/434 NT 99.4% coding (125/1431 nt) Y75_RS17825 YdgA family protein
?NC_007779 = 3748105 NA (NA)intergenic (+146/+3) Y75_RS18585/tnaB IS5‑like element IS5 family transposase/low affinity tryptophan permease TnaB
* ? NC_007779 3748316 =0 (0.000)217 (1.290) 100/434 NT 100% pseudogene (665/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 = 4056987 NA (NA)coding (391/417 nt) Y75_RS20060 hypothetical protein
* ? NC_007779 = 37483241 (0.010)164 (0.980) 88/434 NT 99.4% pseudogene (657/873 nt) tnaB low affinity tryptophan permease TnaB
?NC_007779 4056220 = NA (NA)intergenic (‑209/+15) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
* ? NC_007779 3876427 =NA (NA)4 (0.030) 4/412 NT 16.7% coding (1806/4134 nt) Y75_RS19225 RHS element protein RhsA
?NC_007779 3876545 = 21 (0.130)coding (1688/4134 nt) Y75_RS19225 RHS element protein RhsA
* ? NC_007779 = 39862150 (0.000)259 (1.540) 121/434 NT 100% coding (240/567 nt) slp outer membrane lipoprotein Slp
?NC_007779 3987185 = NA (NA)pseudogene (3/379 nt) Y75_RS19720 hypothetical protein
* ? NC_007779 3987185 =NA (NA)38 (0.230) 34/434 NT 19.5% pseudogene (3/379 nt) Y75_RS19720 hypothetical protein
?NC_007779 4315241 = 157 (0.940)coding (418/1533 nt) Y75_RS21330 D‑allose ABC transporter ATP‑binding protein AlsA
* ? NC_007779 = 4019417131 (0.780)4 (0.030) 4/412 NT 5.4% coding (1807/4236 nt) Y75_RS19860 rhs element protein RhsB
?NC_007779 = 4019535 17 (0.110)coding (1689/4236 nt) Y75_RS19860 rhs element protein RhsB
* ? NC_007779 4056221 =NA (NA)181 (1.080) 97/432 NT 97.3% intergenic (‑210/+14) Y75_RS25055/Y75_RS20055 hypothetical protein/IS1‑like element IS1A family transposase
?NC_007779 4370787 = 5 (0.030)pseudogene (667/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 4056987NA (NA)186 (1.110) 105/434 NT 98.4% coding (391/417 nt) Y75_RS20060 hypothetical protein
?NC_007779 = 4370795 3 (0.020)pseudogene (659/1304 nt) Y75_RS21600 anaerobic C4‑dicarboxylate transporter
* ? NC_007779 = 43103620 (0.000)133 (0.810) 62/426 NT 100% intergenic (‑286/‑33) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 4311562 = 0 (0.000)pseudogene (916/921 nt) alsK allose kinase
* ? NC_007779 = 4311557NA (NA)50 (0.300) 41/434 NT 24.3% pseudogene (921/921 nt) alsK allose kinase
?NC_007779 = 4315244 156 (0.930)coding (415/1533 nt) Y75_RS21330 D‑allose ABC transporter ATP‑binding protein AlsA