breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 87,793 A→C 13.8% E146A (GAG→GCG)  ilvH → acetolactate synthase 3, small subunit, valine‑sensitive
RA 120,158:1 +A 5.9% intergenic (+23/+20) ampE → / ← aroP ampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
RA 125,817 A→G 7.6% K41K (AAA→AAG aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 125,820 C→T 8.1% A42A (GCC→GCT aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,093 T→C 7.2% A133A (GCT→GCC aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,126 G→A 7.4% K144K (AAG→AAA aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 129,802 T→G 12.2% K487Q (AAG→CAG)  yacH ← DUF3300 family protein
RA 129,811 T→G 14.4% T484P (ACT→CCT)  yacH ← DUF3300 family protein
RA 181,274 C→G 6.1% H131D (CAC→GAC) ‡ degP → serine endoprotease (protease Do), membrane‑associated
RA 181,276 C→G 6.3% H131Q (CAC→CAG) ‡ degP → serine endoprotease (protease Do), membrane‑associated
RA 216,073 T→A 5.2% intergenic (+94/+106) nlpE → / ← yaeF lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
RA 216,074 T→A 5.0% intergenic (+95/+105) nlpE → / ← yaeF lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
RA 216,081 C→T 5.1% intergenic (+102/+98) nlpE → / ← yaeF lipoprotein involved with copper homeostasis and adhesion/putative lipoprotein
RA 222,958 Δ1 bp 100% coding (126/576 nt) gmhB → D,D‑heptose 1,7‑bisphosphate phosphatase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 265,573 G→A 5.5% intergenic (‑30/+47) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
RA 265,578 C→T 5.3% intergenic (‑35/+42) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
RA 265,579 T→A 7.1% intergenic (‑36/+41) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
RA 265,582 T→A 6.3% intergenic (‑39/+38) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
RA 265,583 A→G 6.4% intergenic (‑40/+37) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
RA 265,588 T→C 5.0% intergenic (‑45/+32) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
RA 290,247 A→G 6.0% F20S (TTC→TCC)  argF ← ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 304,790 T→G 5.5% E139A (GAG→GCG)  ecpE ← ECP production pilus chaperone
RA 329,493 G→A 5.1% D11N (GAC→AAC)  betT → choline transporter of high affinity
RA 329,512 T→A 5.6% V17E (GTG→GAG)  betT → choline transporter of high affinity
RA 361,220 A→C 6.5% intergenic (+74/+29) cynX → / ← lacA putative cyanate transporter/thiogalactoside acetyltransferase
RA 361,223 G→T 7.0% intergenic (+77/+26) cynX → / ← lacA putative cyanate transporter/thiogalactoside acetyltransferase
RA 361,224 C→G 6.0% intergenic (+78/+25) cynX → / ← lacA putative cyanate transporter/thiogalactoside acetyltransferase
RA 381,302 A→T 7.1% intergenic (‑42/‑49) yaiX ← / → insC1 pseudogene, interrupted by IS2A; hexapeptide transferase superfamily; putative transferase/IS2 repressor TnpA
RA 429,554 A→T 5.6% Y17F (TAT→TTT)  secF → SecYEG protein translocase auxillary subunit
RA 475,971 C→G 6.7% intergenic (+20/+11) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 475,972 C→G 6.4% intergenic (+21/+10) ybaY → / ← ybaZ outer membrane lipoprotein/excision repair protein, alkyltransferase‑like protein ATL
RA 558,385 T→G 6.9% R58R (CGT→CGG sfmA → FimA homolog, function unknown
RA 558,386 A→T 6.9% T59S (ACC→TCC) ‡ sfmA → FimA homolog, function unknown
RA 558,387 C→A 7.4% T59N (ACC→AAC) ‡ sfmA → FimA homolog, function unknown
RA 561,688 G→A 6.7% T664T (ACG→ACA sfmD → putative outer membrane export usher protein
RA 603,393 T→A 5.2% intergenic (+58/+23) pheP → / ← ybdG phenylalanine transporter/mechanosensitive channel protein, miniconductance
RA 824,572 T→A 6.2% intergenic (‑75/‑58) ybhP ← / → ybhQ endo/exonuclease/phosphatase family protein/inner membrane protein
RA 857,578 C→G 5.7% intergenic (+23/+218) ybiT → / ← ybiU ABC‑F family putative regulatory ATPase/DUF1479 family protein
RA 866,388 A→T 6.0% intergenic (‑24/‑180) moeA ← / → iaaA molybdopterin molybdenumtransferase; molybdopterin biosynthesis protein/Isoaspartyl peptidase
RA 942,080 C→T 5.7% I374I (ATC→ATT dmsA → dimethyl sulfoxide reductase, anaerobic, subunit A
RA 985,853 C→T 6.3% intergenic (‑144/+41) aspC ← / ← ompF aspartate aminotransferase, PLP‑dependent/outer membrane porin 1a (Ia;b;F)
RA 985,854 A→T 5.6% intergenic (‑145/+40) aspC ← / ← ompF aspartate aminotransferase, PLP‑dependent/outer membrane porin 1a (Ia;b;F)
RA 985,855 A→T 7.3% intergenic (‑146/+39) aspC ← / ← ompF aspartate aminotransferase, PLP‑dependent/outer membrane porin 1a (Ia;b;F)
RA 985,856 A→G 6.3% intergenic (‑147/+38) aspC ← / ← ompF aspartate aminotransferase, PLP‑dependent/outer membrane porin 1a (Ia;b;F)
RA 1,030,081 A→G 6.0% I6M (ATA→ATG yccX → weak acylphosphatase
RA 1,088,633 C→T 5.4% D409N (GAT→AAT)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
RA 1,134,668 T→A 6.9% intergenic (+111/‑61) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
RA 1,147,980 G→T 7.6% K120N (AAG→AAT plsX → putative phosphate acyltransferase
RA 1,147,986 G→C 7.6% L122L (CTG→CTC plsX → putative phosphate acyltransferase
RA 1,147,987 G→C 7.3% E123Q (GAG→CAG)  plsX → putative phosphate acyltransferase
RA 1,147,993 A→C 7.6% I125L (ATT→CTT)  plsX → putative phosphate acyltransferase
RA 1,159,335 G→T 6.0% intergenic (+33/+27) ptsG → / ← fhuE fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
RA 1,159,336 A→C 5.6% intergenic (+34/+26) ptsG → / ← fhuE fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
RA 1,169,380 T→C 5.7% intergenic (+50/+32) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,169,382 C→G 6.4% intergenic (+52/+30) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,169,384 G→A 5.9% intergenic (+54/+28) bhsA → / ← ldtC biofilm, cell surface and signaling protein/L,D‑transpeptidase linking Lpp to murein
RA 1,232,481 C→T 5.8% intergenic (+27/+19) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
RA 1,232,484 A→G 5.7% intergenic (+30/+16) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
RA 1,286,793 A→G 8.2% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,344,033 A→G 7.5% L105P (CTG→CCG)  yciT ← global regulator of transcription; DeoR family
RA 1,344,036 G→C 7.3% T104S (ACT→AGT)  yciT ← global regulator of transcription; DeoR family
RA 1,344,039 C→T 7.3% R103Q (CGG→CAG)  yciT ← global regulator of transcription; DeoR family
RA 1,429,021 T→A 7.6% intergenic (+37/‑28) lomR → / → stfR pseudogene, Rac prophage lom homolog;Phage or Prophage Related; interrupted by IS5 and N‑ter deletion/Rac prophage; putative tail fiber protein
RA 1,703,251 A→T 6.0% intergenic (+17/+17) add → / ← ydgJ adenosine deaminase/putative oxidoreductase
RA 1,747,155 G→T 14.8% A9S (GCC→TCC)  ydhS → putative oxidoreductase
RA 1,823,335 G→C 7.0% intergenic (+50/‑180) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
RA 1,823,338 G→C 6.3% intergenic (+53/‑177) nadE → / → cho NAD synthetase, NH3/glutamine‑dependent/endonuclease of nucleotide excision repair
RA 1,856,333 T→G 7.0% E199A (GAG→GCG)  ydjH ← putative kinase
RA 1,892,358 C→T 6.1% G293D (GGT→GAT)  yoaA ← putative ATP‑dependent helicase, DinG family
RA 1,892,363 A→G 6.4% Y291Y (TAT→TAC yoaA ← putative ATP‑dependent helicase, DinG family
RA 1,906,229 T→G 5.3% I181S (ATC→AGC)  mntP → putative Mn(2+) efflux pump, mntR‑regulated
RA 1,932,095 T→A 5.3% intergenic (+36/+20) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
RA 1,932,096 C→G 5.5% intergenic (+37/+19) purT → / ← eda phosphoribosylglycinamide formyltransferase 2/KHG/KDPG aldolase; 2‑dehydro‑3‑deoxy‑phosphogluconate/4‑hydroxy‑2‑ oxoglutarate aldolase
RA 1,966,023 A→T 6.2% S57T (TCG→ACG)  flhB ← flagellin export apparatus, substrate specificity protein
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 1,993,582 G→A 5.9% T375T (ACC→ACT uvrC ← excinuclease UvrABC, endonuclease subunit
RA 1,996,373 T→A 6.3% M154L (ATG→TTG)  sdiA ← quorum‑sensing transcriptional activator
RA 1,996,376 T→A 5.4% K153* (AAA→TAA)  sdiA ← quorum‑sensing transcriptional activator
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,081,688 A→T 9.2% I287N (ATT→AAT)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
RA 2,126,155 A→T 5.1% I23N (ATT→AAT)  wcaH ← GDP‑mannose mannosyl hydrolase
RA 2,126,158 A→T 5.8% F22Y (TTT→TAT)  wcaH ← GDP‑mannose mannosyl hydrolase
RA 2,165,264 A→G 5.5% T26A (ACT→GCT) ‡ yegP → UPF0339 family protein
RA 2,165,265 C→A 5.8% T26N (ACT→AAT) ‡ yegP → UPF0339 family protein
RA 2,165,269 C→T 5.6% I27I (ATC→ATT yegP → UPF0339 family protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,242,571 T→A 5.8% N133Y (AAT→TAT)  yeiB ← DUF418 family putative inner membrane protein
RA 2,272,016 T→A 6.0% F431I (TTT→ATT)  rtn → resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
RA 2,311,616 C→T 5.2% intergenic (‑82/+30) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
RA 2,311,619 A→G 5.1% intergenic (‑85/+27) apbE ← / ← ompC putative thiamine‑synthetic flavin transferase lipoprotein/outer membrane porin protein C
RA 2,354,318 C→T 5.7% G18G (GGC→GGT glpB → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
RA 2,357,653 A→G 8.9% L250L (CTA→CTG yfaD → transposase_31 family protein
RA 2,357,665 G→A 7.7% G254G (GGG→GGA yfaD → transposase_31 family protein
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,399,842 C→A 5.7% E108D (GAG→GAT nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,462,677 A→T 5.5% intergenic (+31/‑335) fadL → / → yfdF long‑chain fatty acid outer membrane transporter/uncharacterized protein
RA 2,503,519 C→T 5.7% A323T (GCC→ACC)  fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,503,522 A→G 5.8% Y322H (TAT→CAT)  fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,503,523 A→C 6.8% R321R (CGT→CGG fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,561,205 A→T 5.7% intergenic (+307/‑163) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,596,878:1 +G 5.1% intergenic (‑141/+27) ypfJ ← / ← purC putative neutral zinc metallopeptidase/phosphoribosylaminoimidazole‑succinocarboxamide synthetase
RA 2,691,171 T→A 5.9% noncoding (170/184 nt) glmY ← sRNA activator of glmS mRNA, glmZ processing antagonist
RA 2,700,862 G→A 5.8% P46L (CCG→CTG)  acpS ← holo‑[acyl‑carrier‑protein] synthase 1
RA 2,731,581 G→T 8.5% intergenic (‑424/+19) rrsG ← / ← clpB 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
RA 2,754,944 A→G 6.6% N17D (AAC→GAC) ‡ smpB → tmRNA‑binding trans‑translation protein
RA 2,754,945 A→T 6.7% N17I (AAC→ATC) ‡ smpB → tmRNA‑binding trans‑translation protein
RA 2,754,946 C→T 6.5% N17N (AAC→AAT) ‡ smpB → tmRNA‑binding trans‑translation protein
RA 2,768,609 T→C 9.7% intergenic (+36/‑56) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,768,614 A→C 10.4% intergenic (+41/‑51) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,768,615 G→T 9.0% intergenic (+42/‑50) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,768,620 G→A 8.3% intergenic (+47/‑45) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
RA 2,801,056 A→T 6.1% N31I (AAT→ATT)  nrdI → NrdEF cluster assembly flavodoxin
RA 2,802,654 A→T 5.1% E436V (GAG→GTG)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit
RA 2,804,528 C→A 11.9% intergenic (+67/‑287) nrdF → / → proV ribonucleoside‑diphosphate reductase 2, beta subunit, ferritin‑like protein/glycine betaine/proline ABC transporter periplasmic binding protein
RA 2,842,438 A→C 5.4% intergenic (+24/+135) ascB → / ← hycI cryptic 6‑phospho‑beta‑glucosidase/protease involved in processing C‑terminal end of HycE
RA 2,904,212 G→A 100% intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
RA 2,906,609 G→C 5.1% intergenic (+26/+34) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase
RA 2,960,928 C→G 6.0% G501R (GGC→CGC)  recC ← exonuclease V (RecBCD complex), gamma chain
RA 2,960,930 G→C 5.2% S500C (TCC→TGC)  recC ← exonuclease V (RecBCD complex), gamma chain
RA 2,962,963 G→C 5.3% T65R (ACG→AGG) ‡ ygdB ← DUF2509 family protein
RA 2,962,964 T→G 7.2% T65P (ACG→CCG) ‡ ygdB ← DUF2509 family protein
RA 2,962,966 T→A 5.7% Q64L (CAG→CTG)  ygdB ← DUF2509 family protein
RA 2,976,321 C→A 6.5% noncoding (65/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
RA 3,059,703 A→T 6.2% intergenic (‑2/‑50) yqfE ← / → argP pseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,059,704 A→T 6.0% intergenic (‑3/‑49) yqfE ← / → argP pseudogene, LysR family/transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,061,817 G→C 7.7% W323S (TGG→TCG) ‡ scpA → methylmalonyl‑CoA mutase
RA 3,061,818 G→C 8.3% W323C (TGG→TGC) ‡ scpA → methylmalonyl‑CoA mutase
RA 3,066,310 A→T 5.2% H288Q (CAT→CAA ygfI ← putative DNA‑binding transcriptional regulator
RA 3,092,774:1 +A 7.8% coding (897/951 nt) gshB → glutathione synthetase
RA 3,092,779 Δ1 bp 7.8% coding (902/951 nt) gshB → glutathione synthetase
RA 3,139,639 T→G 5.1% intergenic (+46/+77) yghU → / ← hybG putative S‑transferase/hydrogenase 2 accessory protein
RA 3,156,591 T→G 6.2% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,181,298 A→T 5.4% H293L (CAT→CTT)  ygiC → ATP‑Grasp family ATPase
RA 3,234,507 A→T 5.9% intergenic (‑52/+232) higB ← / ← rlmG mRNA interferase toxin of the HigB‑HigA toxin‑antitoxin system/23S rRNA m(2)G1835 methyltransferase, SAM‑dependent
RA 3,250,989 G→C 7.9% intergenic (+35/‑35) yqjF → / → yqjG putative quinol oxidase subunit/putative S‑transferase
RA 3,250,990 A→T 6.4% intergenic (+36/‑34) yqjF → / → yqjG putative quinol oxidase subunit/putative S‑transferase
RA 3,250,992 G→C 7.7% intergenic (+38/‑32) yqjF → / → yqjG putative quinol oxidase subunit/putative S‑transferase
RA 3,324,952 A→G 5.5% intergenic (‑41/+49) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,955 A→G 5.7% intergenic (‑44/+46) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,956 C→T 5.6% intergenic (‑45/+45) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,959 C→T 5.5% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,368,982 G→C 7.5% intergenic (+28/+32) yhcG → / ← yhcH DUF1016 family protein in the PD‑(D/E)XK nuclease superfamily/DUF386 family protein, cupin superfamily
RA 3,390,512 A→G 5.2% intergenic (+63/+71) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase
RA 3,390,517 A→T 5.5% intergenic (+68/+66) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase
RA 3,390,518 A→T 5.3% intergenic (+69/+65) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase
RA 3,390,519 A→T 5.1% intergenic (+70/+64) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase
RA 3,390,524 C→T 5.4% intergenic (+75/+59) aaeR → / ← tldD transcriptional regulator for aaeXAB operon/putative peptidase
RA 3,407,293 T→A 6.1% intergenic (+27/‑82) accC → / → yhdT acetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
RA 3,423,540 A→G 7.5% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,469,041 A→G 5.9% S271S (AGT→AGC chiA ← periplasmic endochitinase
RA 3,469,050 G→A 5.8% N268N (AAC→AAT chiA ← periplasmic endochitinase
RA 3,544,689 A→T 5.4% R52R (CGT→CGA bioH ← pimeloyl‑ACP methyl ester carboxylesterase
RA 3,548,507 A→G 6.2% F522L (TTC→CTC)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,570,878 A→G 7.1% W148R (TGG→CGG)  glgX ← glycogen debranching enzyme
RA 3,570,880 G→C 7.2% P147R (CCG→CGG)  glgX ← glycogen debranching enzyme
RA 3,570,884 T→C 7.3% T146A (ACG→GCG)  glgX ← glycogen debranching enzyme
RA 3,570,885 G→A 7.4% R145R (CGC→CGT glgX ← glycogen debranching enzyme
RA 3,570,889 G→C 7.3% P144R (CCG→CGG)  glgX ← glycogen debranching enzyme
RA 3,570,891 C→T 7.7% P143P (CCG→CCA glgX ← glycogen debranching enzyme
RA 3,642,358 T→A 5.5% intergenic (+27/+22) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,798,234 A→T 5.2% intergenic (+27/+5) waaL → / ← waaU O‑antigen ligase/lipopolysaccharide core biosynthesis
RA 3,798,235 A→T 5.1% intergenic (+28/+4) waaL → / ← waaU O‑antigen ligase/lipopolysaccharide core biosynthesis
RA 3,809,354 A→G 5.8% E272G (GAA→GGA)  waaA → 3‑deoxy‑D‑manno‑octulosonic‑acid transferase (KDO transferase)
JC 3,815,859 Δ82 bp 35.8% [rph][rph] [rph], [rph]
JC 3,815,899 Δ8 bp 19.8% pseudogene (644‑651/669 nt) rph ← ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,891,502 C→G 5.1% intergenic (+19/‑113) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,504 T→A 5.3% intergenic (+21/‑111) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,897,613 G→A 7.2% P36P (CCG→CCA cbrB → PRK09823 family inner membrane protein, creBC regulon
RA 3,897,614 C→G 6.4% L37V (CTG→GTG) ‡ cbrB → PRK09823 family inner membrane protein, creBC regulon
RA 3,897,615 T→C 6.4% L37P (CTG→CCG) ‡ cbrB → PRK09823 family inner membrane protein, creBC regulon
RA 3,915,514 C→T 6.1% intergenic (‑314/+39) glmU ← / ← atpC fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/F1 sector of membrane‑bound ATP synthase, epsilon subunit
RA 3,915,519 T→A 6.0% intergenic (‑319/+34) glmU ← / ← atpC fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/F1 sector of membrane‑bound ATP synthase, epsilon subunit
RA 3,915,520 T→A 5.6% intergenic (‑320/+33) glmU ← / ← atpC fused N‑acetyl glucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyl transferase/F1 sector of membrane‑bound ATP synthase, epsilon subunit
RA 3,959,515 T→A 6.9% intergenic (+70/+17) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 3,959,924 A→T 5.1% intergenic (‑111/+88) ppiC ← / ← yifN peptidyl‑prolyl cis‑trans isomerase C (rotamase C)/PemK toxin family pseudogene
RA 3,969,429 A→C 5.8% K133N (AAA→AAC wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,969,434 A→C 5.8% D135A (GAT→GCT)  wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,969,436 G→T 5.5% A136S (GCG→TCG)  wzzE → Entobacterial Common Antigen (ECA) polysaccharide chain length modulation protein
RA 3,998,147 C→A 5.3% Y55* (TAC→TAA uvrD → DNA‑dependent ATPase I and helicase II
RA 4,041,221 G→A 5.0% A71A (GCC→GCT mobB ← molybdopterin‑guanine dinucleotide biosynthesis protein B
RA 4,056,038 T→G 8.1% D101A (GAC→GCC)  glnL ← sensory histidine kinase in two‑component regulatory system with GlnG
RA 4,118,829 T→C 5.2% intergenic (+69/+16) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
RA 4,118,830 G→A 5.1% intergenic (+70/+15) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
RA 4,118,831 C→A 5.4% intergenic (+71/+14) zapB → / ← rraA FtsZ stabilizer, septal ring assembly factor, cell division stimulator/ribonuclease E (RNase E) inhibitor protein
RA 4,148,049 C→G 5.1% G90A (GGC→GCC)  yijO ← AraC family putative transcriptional activator
JC 4,183,399 Δ3 bp 9.4% coding (2155‑2157/4029 nt) rpoB → RNA polymerase, beta subunit
RA 4,193,286 G→C 5.8% D97E (GAC→GAG thiF ← adenylyltransferase, modifies ThiS C‑terminus
RA 4,277,223 A→T 6.3% I54N (ATT→AAT)  soxS ← superoxide response regulon transcriptional activator; autoregulator
RA 4,277,225 G→A 5.7% Y53Y (TAC→TAT) ‡ soxS ← superoxide response regulon transcriptional activator; autoregulator
RA 4,277,226 T→C 5.9% Y53C (TAC→TGC) ‡ soxS ← superoxide response regulon transcriptional activator; autoregulator
RA 4,296,060 C→T 38.9% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,297,196 A→C 6.6% intergenic (‑71/+23) yjcO ← / ← fdhF Sel1 family TPR‑like repeat protein/formate dehydrogenase‑H, selenopolypeptide subunit
RA 4,317,985 T→A 5.2% intergenic (‑90/+21) phnL ← / ← phnK ribophosphonate triphosphate synthase subunit; putative ABC transporter‑related ATPase/carbon‑phosphorus lyase complex subunit, putative ATP transporter ATP‑binding protein
RA 4,326,058 G→A 7.6% intergenic (‑317/+341) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,059 C→A 6.7% intergenic (‑318/+340) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,065 A→T 8.8% intergenic (‑324/+334) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,066 A→T 6.5% intergenic (‑325/+333) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,072 T→G 7.6% intergenic (‑331/+327) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,073 T→C 7.3% intergenic (‑332/+326) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,339,802 A→G 5.4% A240A (GCT→GCC adiA ← arginine decarboxylase
RA 4,339,805 C→T 5.3% Q239Q (CAG→CAA adiA ← arginine decarboxylase
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
RA 4,470,958 G→T 5.7% intergenic (‑45/+28) ridA ← / ← pyrI enamine/imine deaminase, reaction intermediate detoxification/aspartate carbamoyltransferase, regulatory subunit
RA 4,476,080 G→T 6.7% G215V (GGG→GTG)  yjgL → SopA‑central‑domain‑like hexapeptide repeat protein
RA 4,484,461 A→C 5.7% A497A (GCT→GCG pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,499,885 C→G 5.1% pseudogene (650/942 nt) yjgX ← pseudogene fragment
RA 4,499,887 G→C 5.0% pseudogene (648/942 nt) yjgX ← pseudogene fragment
RA 4,520,500 Δ1 bp 5.3% intergenic (‑176/+171) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,520,504:1 +T 5.2% intergenic (‑180/+167) yjhU ← / ← yjhF putative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
RA 4,593,431 A→G 5.2% V431A (GTC→GCC)  lgoT ← putative L‑galactonate:H+ symporter
RA 4,593,434 C→T 5.4% C430Y (TGC→TAC)  lgoT ← putative L‑galactonate:H+ symporter
RA 4,633,634 C→T 100% E38K (GAG→AAG)  yjjX ← non‑canonical purine NTP phosphatase, ITPase/XTPase
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 772991 774198 1208 41 [38] [38] 39 [cydA]–[cydX] [cydA],cydB,[cydX]
* * ÷ NC_000913 1037678 1039354 1677 39 [38] [38] 39 cbdA–[cbdB] cbdA,[cbdB]
* * ÷ NC_000913 1166053 1167428 1376 39 [38] [37] 39 ndh ndh
* * ÷ NC_000913 3423738–3424234 3424577–3424238 5–840 39 [38] [38] 39 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 5565 =NA (NA)3 (0.030) 3/262 NT NA noncoding (1/105 nt) RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 5597 = NA (NA)noncoding (33/105 nt) RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP1 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 = 16653NA (NA)7 (0.080) 6/262 NT NA noncoding (1267/1345 nt) IS186 repeat region
?NC_000913 = 16691 NA (NA)noncoding (1305/1345 nt) IS186 repeat region
* ? NC_000913 = 9319993 (1.030)5 (0.060) 3/266 NT 5.1% coding (34/1488 nt) murE UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase
?NC_000913 = 93223 96 (1.100)coding (58/1488 nt) murE UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate:meso‑ diaminopimelate ligase
* ? NC_000913 274347 =NA (NA)4 (0.050) 4/262 NT NA noncoding (803/1195 nt) IS5 repeat region
?NC_000913 274410 = NA (NA)noncoding (740/1195 nt) IS5 repeat region
* ? NC_000913 274620 =NA (NA)3 (0.040) 3/258 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 = 274695NA (NA)4 (0.050) 3/268 NT NA noncoding (455/1195 nt) IS5 repeat region
?NC_000913 = 274727 NA (NA)noncoding (423/1195 nt) IS5 repeat region
* ? NC_000913 381663 =NA (NA)6 (0.070) 4/262 NT NA noncoding (404/1331 nt) IS2 repeat region
?NC_000913 381703 = NA (NA)noncoding (444/1331 nt) IS2 repeat region
* ? NC_000913 = 381669NA (NA)6 (0.070) 5/262 NT NA noncoding (410/1331 nt) IS2 repeat region
?NC_000913 = 381695 NA (NA)noncoding (436/1331 nt) IS2 repeat region
* ? NC_000913 412077 =103 (1.140)5 (0.060) 4/260 NT 5.2% coding (405/1185 nt) araJ L‑arabinose‑inducible putative transporter, MFS family
?NC_000913 412119 = 87 (1.020)coding (363/1185 nt) araJ L‑arabinose‑inducible putative transporter, MFS family
* ? NC_000913 = 52182887 (0.960)5 (0.060) 5/258 NT 5.5% coding (1413/2415 nt) ybbP putative ABC transporter permease
?NC_000913 = 521858 90 (1.060)coding (1443/2415 nt) ybbP putative ABC transporter permease
* ? NC_000913 = 53551485 (0.940)5 (0.060) 5/252 NT 5.8% coding (1599/1782 nt) gcl glyoxylate carboligase
?NC_000913 = 535539 86 (1.040)coding (1624/1782 nt) gcl glyoxylate carboligase
* ? NC_000913 = 730045NA (NA)3 (0.030) 3/266 NT NA coding (463/4194 nt) rhsC Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 730067 NA (NA)coding (485/4194 nt) rhsC Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 864470 =110 (1.210)6 (0.070) 4/264 NT 5.6% coding (660/750 nt) moeB molybdopterin synthase sulfurylase
?NC_000913 864498 = 97 (1.120)coding (632/750 nt) moeB molybdopterin synthase sulfurylase
* ? NC_000913 1207790 =27 (0.300)21 (0.260) 19/244 NT 49.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 19 (0.240)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780524 (0.270)41 (0.510) 35/244 NT 67.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 19 (0.240)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)129 (1.470) 100/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 144044390 (0.990)6 (0.070) 5/262 NT 6.6% coding (342/3525 nt) pfo pyruvate‑flavodoxin oxidoreductase
?NC_000913 = 1440464 85 (0.990)coding (321/3525 nt) pfo pyruvate‑flavodoxin oxidoreductase
* ? NC_000913 = 148496769 (0.760)4 (0.050) 4/258 NT 5.7% coding (1907/3903 nt) hrpA putative ATP‑dependent helicase
?NC_000913 = 1484972 69 (0.820)coding (1912/3903 nt) hrpA putative ATP‑dependent helicase
* ? NC_000913 = 155395076 (0.840)5 (0.060) 5/258 NT 6.6% intergenic (‑112/+22) adhP/maeA ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
?NC_000913 = 1553956 71 (0.840)intergenic (‑118/+16) adhP/maeA ethanol‑active dehydrogenase/acetaldehyde‑active reductase/malate dehydrogenase, decarboxylating, NAD‑requiring; malic enzyme
* ? NC_000913 1841735 =72 (0.800)4 (0.050) 3/264 NT 5.2% coding (246/408 nt) nudG CTP pyrophosphohydrolase; also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
?NC_000913 1841775 = 77 (0.890)coding (286/408 nt) nudG CTP pyrophosphohydrolase; also hydrolyzes 2‑hydroxy‑dATP, 8‑hydroxy‑dGTP, 5‑hydroxy‑CTP, dCTP and 5‑methyl‑dCTP
* ? NC_000913 = 190927682 (0.910)5 (0.060) 4/254 NT 6.0% intergenic (+112/+32) yobH/kdgR uncharacterized protein/KDG regulon transcriptional repressor
?NC_000913 = 1909289 80 (0.960)intergenic (+125/+19) yobH/kdgR uncharacterized protein/KDG regulon transcriptional repressor
* ? NC_000913 = 199308764 (0.710)5 (0.060) 4/266 NT 7.4% coding (1620/1833 nt) uvrC excinuclease UvrABC, endonuclease subunit
?NC_000913 = 1993103 64 (0.730)coding (1604/1833 nt) uvrC excinuclease UvrABC, endonuclease subunit
* ? NC_000913 = 2021654100 (1.100)6 (0.070) 5/258 NT 6.0% coding (151/366 nt) fliO flagellar biosynthesis protein
?NC_000913 = 2021673 95 (1.120)coding (170/366 nt) fliO flagellar biosynthesis protein
* ? NC_000913 2250781 =70 (0.770)5 (0.060) 4/256 NT 7.3% intergenic (+15/‑59) yeiH/nfo UPF0324 family inner membrane protein/endonuclease IV with intrinsic 3'‑5' exonuclease activity
?NC_000913 2250833 = 62 (0.740)intergenic (+67/‑7) yeiH/nfo UPF0324 family inner membrane protein/endonuclease IV with intrinsic 3'‑5' exonuclease activity
* ? NC_000913 2440343 =NA (NA)3 (0.030) 3/264 NT NA noncoding (1/36 nt) REP170 (repetitive extragenic palindromic) element; contains 1 REP sequences REP170 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 2440375 = NA (NA)noncoding (33/36 nt) REP170 (repetitive extragenic palindromic) element; contains 1 REP sequences REP170 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? NC_000913 2726200 =NA (NA)6 (0.070) 6/264 NT NA intergenic (‑12/+81) rrfG/rrlG 5S ribosomal RNA of rrnG operon/23S ribosomal RNA of rrnG operon
?NC_000913 2726224 = NA (NA)intergenic (‑36/+57) rrfG/rrlG 5S ribosomal RNA of rrnG operon/23S ribosomal RNA of rrnG operon
* ? NC_000913 = 342355492 (1.020)7 (0.080) 6/274 NT 7.1% intergenic (‑12/+26) rrfF/thrV 5S ribosomal RNA of rrnD operon/tRNA‑Thr
?NC_000913 4171603 = NA (NA)intergenic (+59/‑34) rrlB/rrfB 23S ribosomal RNA of rrnB operon/5S ribosomal RNA of rrnB operon
* ? NC_000913 = 3620220NA (NA)4 (0.050) 4/264 NT 100% coding (1029/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620255 0 (0.000)coding (1064/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 3620363NA (NA)3 (0.030) 3/266 NT 100% coding (1172/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 3620412 0 (0.000)coding (1221/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 3700159122 (1.350)7 (0.080) 5/264 NT 5.5% noncoding (24/66 nt) rdlD sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD
?NC_000913 = 3700189 124 (1.430)noncoding (54/66 nt) rdlD sRNA antisense regulator affects LdrD translation; proposed addiction module in LDR‑D repeat, with toxic peptide LdrD
* ? NC_000913 3910443 =NA (NA)4 (0.050) 3/266 NT NA noncoding (1/35 nt) REP284 (repetitive extragenic palindromic) element; contains 1 REP sequences REP284 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 3910474 = NA (NA)noncoding (32/35 nt) REP284 (repetitive extragenic palindromic) element; contains 1 REP sequences REP284 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? NC_000913 = 4040481NA (NA)8 (0.090) 6/264 NT 22.4% intergenic (+58/‑36) rrlA/rrfA 23S ribosomal RNA of rrnA operon/5S ribosomal RNA of rrnA operon
?NC_000913 = 4040505 29 (0.320)intergenic (+82/‑12) rrlA/rrfA 23S ribosomal RNA of rrnA operon/5S ribosomal RNA of rrnA operon
* ? NC_000913 = 416623069 (0.760)6 (0.070) 4/268 NT 7.8% coding (803/858 nt) murI glutamate racemase
?NC_000913 = 4166269 75 (0.850)coding (842/858 nt) murI glutamate racemase
* ? NC_000913 4284390 =54 (0.600)6 (0.070) 5/262 NT 10.8% coding (513/1650 nt) actP acetate transporter
?NC_000913 4284425 = 48 (0.560)coding (478/1650 nt) actP acetate transporter