breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 45,294 A→G N372S (AAC→AGC)  fixC → putative oxidoreductase FixC
RA 55,528 A→G Y528H (TAC→CAC)  lptD ← lipopolysaccharide assembly protein LptD
RA 110,478 A→G T734A (ACG→GCG)  secA → protein translocation ATPase
RA 114,800 T→C N309D (AAC→GAC)  hofC ← inner membrane protein HofC
RA 158,295 T→G T278P (ACC→CCC)  pcnB ← poly(A) polymerase I
RA 169,707 A→G T742A (ACC→GCC)  fhuA → ferrichrome outer membrane transporter/phage receptor
RA 170,273 A→G S166G (AGC→GGC)  fhuC → iron(III) hydroxamate ABC transporter ATP binding subunit
RA 187,736 A→G G305G (GGT→GGC glnD ← PII uridylyltransferase/uridylyl removing enzyme
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 263,502 A→G G56G (GGT→GGC ykfI ← CP4‑6 prophage; toxin of the YkfI‑YafW toxin‑antitoxin system
RA 303,093 (G)6→7 coding (103/615 nt) yagU → inner membrane protein that contributes to acid resistance
RA 321,564 (G)7→8 intergenic (+483/‑44) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 374,141 A→G T92A (ACT→GCT)  mhpE → 4‑hydroxy‑2‑oxovalerate aldolase
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 395,146 A→G V381A (GTT→GCT)  ampH ← peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
RA 416,750 A→G V68A (GTC→GCC)  sbcD ← ATP dependent, structure specific DNA nuclease ‑ SbcD subunit
RA 425,420 T→C V137A (GTG→GCG)  queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase
RA 427,546 A→G D87G (GAC→GGC)  yajC → Sec translocon accessory complex subunit YajC
RA 451,331 T→C T94A (ACG→GCG)  cyoA ← cytochrome bo3 ubiquinol oxidase subunit 2
RA 477,402 C→T A406T (GCC→ACC)  pdeB ← c‑di‑GMP phosphodiesterase PdeB
RA 484,885 T→C S245S (TCA→TCG acrA ← multidrug efflux pump membrane fusion lipoprotein AcrA
RA 503,672 T→C E342G (GAG→GGG)  fsr ← fosmidomycin efflux pump
RA 506,730 (G)5→4 coding (353/480 nt) ybaK ← Cys‑tRNA(Pro) and Cys‑tRNA(Cys) deacylase
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 539,690 C→T P182S (CCG→TCG)  allB → allantoinase
RA 544,463 A→G V277A (GTG→GCG)  allC ← allantoate amidohydrolase
RA 563,450 A→G T41A (ACC→GCC)  sfmF → putative fimbrial protein SfmF
RA 564,661 C→A intergenic (‑181/‑62) fimZ ← / → argU putative LuxR family transcriptional regulator FimZ/tRNA‑Arg
RA 571,587 A→G E45G (GAG→GGG)  ybcM → DLP12 prophage; putative DNA‑binding transcriptional regulator
RA 607,626 T→C intergenic (‑243/‑108) ybdK ← / → sokE carboxylate‑amine ligase/small RNA SokE
RA 614,362 T→C V69A (GTC→GCC)  entF → apo‑serine activating enzyme
RA 629,579 T→C S94P (TCT→CCT)  entH → proofreading thioesterase in enterobactin biosynthesis
RA 682,304 A→G L364L (TTG→CTG)  hscC ← chaperone protein HscC
RA 706,889 A→G Y266C (TAC→TGC)  glnS → glutamine‑‑tRNA ligase
RA 723,528 (T)8→7 coding (887/2685 nt) kdpD ← sensory histidine kinase KdpD
RA 731,859 T→C H759H (CAT→CAC rhsC → rhs element protein RhsC
RA 731,869 A→G N763E (AAT→GAA)  rhsC → rhs element protein RhsC
RA 731,871 T→A N763E (AAT→GAA rhsC → rhs element protein RhsC
RA 731,873 A→C E764A (GAA→GCA)  rhsC → rhs element protein RhsC
RA 731,913 A→G A777A (GCA→GCG rhsC → rhs element protein RhsC
RA 731,919 A→G G779G (GGA→GGG rhsC → rhs element protein RhsC
RA 735,023 C→G pseudogene (804/1434 nt) ybfO → conserved protein, rhs‑like YbfO
RA 766,957 (G)6→7 coding (974/1977 nt) mngA → 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
RA 787,470 A→G G42G (GGT→GGC gpmA ← 2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
RA 796,249 G→A G130R (GGG→AGG)  modB → molybdate ABC transporter membrane subunit
RA 830,264 A→G L127P (CTC→CCC)  cecR ← DNA‑binding transcriptional dual regulator CecR
RA 837,737 A→G G63G (GGT→GGC ybiJ ← DUF1471 domain‑containing protein YbiJ
RA 855,968 A→G L2L (TTA→TTG ybiT → ABC transporter ATP‑binding protein YbiT
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 900,275 T→C T101A (ACC→GCC)  artJ ← L‑arginine ABC transporter periplasmic binding protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 933,443 A→G T74A (ACG→GCG)  ftsK → cell division DNA translocase FtsK
RA 935,434 A→G E737E (GAA→GAG ftsK → cell division DNA translocase FtsK
RA 939,183 A→G N397S (AAC→AGC)  rarA → recombination factor
RA 954,097 C→T G124S (GGC→AGC)  focA ← formate channel FocA
RA 987,008 (T)8→7 intergenic (‑26/+577) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 1,003,357 Δ1 bp coding (469/543 nt) ycbU → putative fimbrial protein YcbU
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,016,118 A→G V118A (GTA→GCA)  fabA ← beta‑hydroxyacyl‑acyl carrier protein dehydratase/isomerase
RA 1,019,811 A→G R81R (CGT→CGC ompA ← outer membrane porin A
RA 1,056,094 A→G Y29H (TAC→CAC)  torS ← sensory histidine kinase TorS
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,148,063 G→A G148E (GGG→GAG)  plsX → putative phosphate acyltransferase
RA 1,181,071 T→C D66G (GAC→GGC)  ycfZ ← putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,213,369 A→G W231R (TGG→CGG)  bluR ← DNA‑binding transcriptional repressor BluR
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,245,412 (A)7→8 coding (253/441 nt) ycgY → uncharacterized protein YcgY
RA 1,249,873 T→C S322S (TCA→TCG dhaK ← dihydroxyacetone kinase subunit K
RA 1,264,631 C→T I306I (ATC→ATT hemA → glutamyl‑tRNA reductase
RA 1,272,154 C→T S12S (AGC→AGT chaB → putative cation transport regulator ChaB
RA 1,280,952 T→C D363D (GAT→GAC narG → nitrate reductase A subunit alpha
RA 1,295,063 Δ1 bp pseudogene (260/897 nt) insZ ← putative transposase InsZ
RA 1,295,065 C→T pseudogene (258/897 nt) insZ ← putative transposase InsZ
RA 1,305,170 A→G K139K (AAA→AAG oppD → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppD
JC 1,318,571 (C)6→9 coding (1218/1362 nt) trpC ← fused indole‑3‑glycerol phosphate synthase/phosphoribosylanthranilate isomerase
RA 1,351,196 (G)7→6 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,391,095 T→C A253A (GCA→GCG ycjY ← putative hydrolase YcjY
RA 1,414,119 C→T L225L (CTG→CTA recT ← Rac prophage; recombinase, DNA renaturation
RA 1,415,222 A→G V722A (GTT→GCT)  recE ← Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' ‑‑> 3' specific
RA 1,428,923 G→A pseudogene (128/189 nt) lomR → Rac prophage; protein LomR_1
RA 1,439,190 A→G V532A (GTG→GCG)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,451,628 A→G V672A (GTG→GCG)  paaZ ← oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,458,575 T→C C104C (TGT→TGC paaG → putative ring 1,2‑epoxyphenylacetyl‑CoA isomerase (oxepin‑CoA forming)
RA 1,472,600 T→C pseudogene (2082/3495 nt) ydbA → putative outer membrane protein, N‑terminal fragment
RA 1,509,335 T→C V17A (GTA→GCA)  hicA → toxin of the HicA‑HicB toxin‑antitoxin system
RA 1,535,998 A→G V207A (GTG→GCG)  narV ← nitrate reductase Z subunit gamma
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,545,775 T→C N85S (AAC→AGC)  yddL ← putative uncharacterized protein YddL
RA 1,569,819 A→G V224A (GTC→GCC)  gadC ← L‑glutamate:4‑aminobutyrate antiporter
RA 1,584,611 T→C T626A (ACC→GCC)  ydeP ← putative oxidoreductase YdeP
RA 1,586,334 A→G G51G (GGT→GGC ydeP ← putative oxidoreductase YdeP
RA 1,592,389 T→C K18K (AAA→AAG hipB ← antitoxin/DNA‑binding transcriptional repressor HipB
RA 1,594,132 (C)8→7 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,619,930 A→G L119L (TTA→TTG marA → DNA‑binding transcriptional dual regulator MarA
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,646,935 A→G intergenic (+198/+239) flxA → / ← ydfW Qin prophage; protein FlxA/Qin prophage; uncharacterized protein YdfW
RA 1,661,045 A→G T164A (ACA→GCA)  ynfF → putative selenate reductase YnfF
RA 1,662,588 A→G D678G (GAT→GGT)  ynfF → putative selenate reductase YnfF
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,692,183 (C)5→4 coding (669/1266 nt) uidC ← outer membrane porin family protein
RA 1,705,392 G→A G48D (GGC→GAC)  ydgK → conserved inner membrane protein YdgK
RA 1,717,008 A→G V72A (GTA→GCA)  tyrS ← tyrosine‑‑tRNA ligase
RA 1,718,882 T→C T241A (ACC→GCC)  anmK ← anhydro‑N‑acetylmuramic acid kinase
RA 1,732,427 T→C V1114A (GTA→GCA)  lhr → putative ATP‑dependent helicase Lhr
RA 1,742,580 (C)5→6 intergenic (+19/+21) cfa → / ← ribC cyclopropane fatty acyl phospholipid synthase/riboflavin synthase
RA 1,752,975 T→C T286A (ACC→GCC)  ydhV ← putative oxidoreductase
RA 1,795,493 C→T K20K (AAG→AAA ihfA ← integration host factor subunit alpha
RA 1,847,303 G→A H236Y (CAC→TAC)  selD ← selenide, water dikinase
RA 1,852,912 T→C G356G (GGA→GGG ydjE ← putative transporter YdjE
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,882,567 A→G D219G (GAC→GGC)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,906,322 T→C T247A (ACA→GCA)  rlmA ← 23S rRNA m(1)G745 methyltransferase
RA 1,921,384 G→A A388T (GCC→ACC)  rsmF → 16S rRNA m(5)C1407 methyltransferase
RA 1,933,049 A→G V519A (GTG→GCG)  edd ← phosphogluconate dehydratase
RA 1,947,931 A→G V74A (GTG→GCG)  yebC ← putative transcriptional regulator YebC
RA 1,949,210 T→C D438G (GAC→GGC)  aspS ← aspartate‑‑tRNA ligase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,996,183 T→C N217S (AAC→AGC)  sdiA ← DNA‑binding transcriptional dual regulator SdiA
RA 2,019,197 T→C V271A (GTG→GCG)  fliK → flagellar hook‑length control protein
RA 2,024,716 C→T S36S (TCG→TCA dsrB ← protein DsrB
RA 2,104,882 (A)9→8 coding (203/591 nt) wbbJ ← putative acyl transferase
RA 2,110,586 C→T R145H (CGC→CAC)  rfbA ← dTDP‑glucose pyrophosphorylase
RA 2,117,460 T→C E315E (GAA→GAG wcaK ← putative colanic acid biosynthesis pyruvyl transferase
RA 2,143,095 (G)5→6 intergenic (‑147/‑171) udk ← / → dgcE uridine/cytidine kinase/putative diguanylate cyclase DgcE
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,227,340 (C)6→7 pseudogene (1419/1436 nt) mdtQ ← putative multidrug resistance outer membrane protein MdtQ
RA 2,231,507 T→C H163H (CAT→CAC yohK → PF04712 family membrane protein YohK
RA 2,275,075 (T)6→5 coding (897/1095 nt) yejB → putative oligopeptide ABC transporter membrane subunit YejB
RA 2,321,433 T→C V464A (GTT→GCT)  atoS → sensory histidine kinase AtoS
RA 2,330,974 G→A pseudogene (3429/4605 nt) yfaS ← putative macroglobulin family protein, N‑terminal fragment
RA 2,336,818 A→G V868A (GTT→GCT)  gyrA ← DNA gyrase subunit A
RA 2,342,105 T→C T689A (ACC→GCC)  yfaL ← putative autotransporter adhesin YfaL
RA 2,357,848 A→G pseudogene (33/186 nt) ypaA → protein YpaA
RA 2,400,589 T→C T323A (ACG→GCG)  nuoF ← NADH:quinone oxidoreductase subunit F
RA 2,407,653 A→G E31E (GAA→GAG alaA → glutamate‑‑pyruvate aminotransferase AlaA
RA 2,421,167 T→C Y183H (TAT→CAT)  yfcG → disulfide reductase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,462,950 (T)6→5 intergenic (+304/‑62) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,473,773 A→G N57S (AAC→AGC)  yfdP → CPS‑53 (KpLE1) prophage; protein YfdP
RA 2,482,011 T→C T56A (ACC→GCC)  emrY ← tripartite efflux pump membrane subunit EmrY
RA 2,488,994 C→T G59R (GGA→AGA)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 2,490,407 T→C N515S (AAC→AGC)  oxc ← oxalyl‑CoA decarboxylase
RA 2,511,786 T→C T307T (ACA→ACG mntH ← Mn(2(+))/Fe(2(+)): H(+) symporter MntH
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,533,577 T→C intergenic (+197/‑187) cysK → / → ptsH O‑acetylserine sulfhydrylase A/phosphocarrier protein HPr
RA 2,561,765 T→C C47C (TGT→TGC yffN → CPZ‑55 prophage; uncharacterized protein YffN
RA 2,576,882 A→G V499A (GTC→GCC)  maeB ← malate dehydrogenase
RA 2,675,642 G→A C40C (TGC→TGT yphC ← putative zinc‑binding dehydrogenase YphC
RA 2,695,585 (G)7→8 intergenic (‑42/‑216) purL ← / → mltF phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F
RA 2,722,210 A→G Y753C (TAC→TGC)  patZ → peptidyl‑lysine acetyltransferase
RA 2,748,674 T→C V238A (GTC→GCC)  ypjD → cytochrome c assembly family protein
RA 2,765,794 (C)7→6 intergenic (‑18/‑124) yfjM ← / → rnlA CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,793,078 T→C V115A (GTG→GCG)  gabT → 4‑aminobutyrate aminotransferase GabT
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,830,289 T→C N159N (AAT→AAC gutQ → D‑arabinose 5‑phosphate isomerase GutQ
RA 2,840,034 A→G I171V (ATC→GTC)  ascF → beta‑glucoside specific PTS enzyme IIBC component
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,870,786 (C)5→6 coding (519/1050 nt) truD ← tRNA pseudouridine(13) synthase
RA 2,900,306 (A)9→8 intergenic (‑33/‑286) ygcW ← / → yqcE putative deoxygluconate dehydrogenase/putative transport protein YqcE
RA 2,902,347 A→G D151G (GAT→GGT)  ygcE → putative sugar kinase YgcE
RA 2,903,376 T→C intergenic (+2/+1371) ygcE → / ← queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,906,811 G→A D377D (GAC→GAT eno ← enolase
RA 2,942,289 (C)5→6 coding (279/918 nt) gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,957,264 A→G Y541H (TAT→CAT)  ptrA ← protease 3
RA 2,964,480 G→A P226S (CCG→TCG)  thyA ← thymidylate synthase
RA 2,990,286 T→C K25K (AAA→AAG yqeK ← protein YqeK
RA 2,994,202 (A)7→6 pseudogene (264/503 nt) pbl → transglycosylase domain‑containing protein Pbl
RA 3,000,529 T→C V62A (GTG→GCG)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,013,537 T→C T235A (ACG→GCG)  yqeB ← XdhC‑CoxI family protein YqeB
RA 3,022,651 G→A G446S (GGT→AGT)  xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,028,788 G→A G75G (GGC→GGT ygfS ← putative oxidoreductase, 4Fe‑4S ferredoxin‑type subunit
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,076,176 A→G S3P (TCC→CCC)  yggF ← fructose 1,6‑bisphosphatase YggF
RA 3,081,769 T→C intergenic (‑134/‑144) tktA ← / → loiP transketolase 1/metalloprotease LoiP
RA 3,084,908 T→C Y335C (TAC→TGC)  speA ← arginine decarboxylase, biosynthetic
RA 3,118,282 A→G G277G (GGT→GGC yghJ ← putative lipoprotein YghJ
RA 3,130,114 Δ1 bp coding (102/1173 nt) yghO ← putative DNA‑binding transcriptional regulator YghO
RA 3,133,542 G→A P139L (CCG→CTG)  yghS ← putative ATP‑binding protein YghS
RA 3,140,431 G→A G123G (GGC→GGT hybE ← hydrogenase 2‑specific chaperone
RA 3,192,722 T→C L172P (CTA→CCA)  yqiJ → putative inner membrane protein
RA 3,240,984 (G)7→6 coding (1041/1245 nt) sstT → serine/threonine:Na(+) symporter
RA 3,246,323 G→A G367E (GGG→GAG)  exuT → hexuronate transporter
RA 3,268,018 A→G E55E (GAA→GAG yhaB → protein YhaB
RA 3,283,461 C→T H107Y (CAC→TAC)  kbaY → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaY
RA 3,311,807 G→A intergenic (‑123/+26) rpsO ← / ← truB 30S ribosomal subunit protein S15/tRNA pseudouridine(55) synthase
RA 3,328,883 T→C intergenic (‑168/‑80) greA ← / → dacB transcription elongation factor GreA/peptidoglycan DD‑endopeptidase DacB
RA 3,331,902 C→T M278I (ATG→ATA yhbE ← inner membrane protein YhbE
RA 3,344,165 A→G A74A (GCA→GCG lptB → lipopolysaccharide transport system ATP binding protein
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,375,656 A→G H263R (CAC→CGC)  dcuD → putative transporter DcuD
RA 3,384,864 T→C G54G (GGT→GGC argR → DNA‑binding transcriptional dual regulator ArgR
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,411,866 T→C C72R (TGC→CGC)  yhdJ → DNA adenine methyltransferase
RA 3,422,782 T→C V116A (GTA→GCA)  yhdZ → putative ABC transporter ATP‑binding subunit YhdZ
RA 3,423,705 +C noncoding (83/120 nt) rrfD ← 5S ribosomal RNA
RA 3,465,960 T→C C140R (TGT→CGT)  gspO → Type II secretion system prepilin peptidase
RA 3,503,538 A→G G124G (GGA→GGG frlD → fructoselysine 6‑kinase
RA 3,507,384 T→C E106E (GAA→GAG yhfU ← DUF2620 domain‑containing protein YhfU
RA 3,546,546 G→A intergenic (+347/‑13) nfuA → / → gntT iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,587,714 G→A R134C (CGC→TGC)  ugpQ ← glycerophosphodiester phosphodiesterase
RA 3,622,960 (G)5→6 coding (3769/4236 nt) rhsB → rhs element protein RhsB
RA 3,627,336 C→T A735T (GCC→ACC)  rbbA ← ribosome‑associated ATPase
RA 3,642,649 T→C T162A (ACC→GCC)  rsmJ ← 16S rRNA m(2)G1516 methyltransferase
RA 3,728,118 (C)8→7 coding (1254/1455 nt) xylB ← xylulokinase
RA 3,744,442 G→A E39K (GAA→AAA)  yiaM → 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
RA 3,767,186 T→C intergenic (+7/‑35) yibA → / → yibJ putative lyase containing HEAT‑repeat/putative RHS domain‑containing protein YibJ
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,877,479 A→G intergenic (‑14/+226) yidB ← / ← gyrB conserved protein YidB/DNA gyrase subunit B
RA 3,879,714 G→A R136C (CGC→TGC)  gyrB ← DNA gyrase subunit B
RA 3,894,463 T→C W195R (TGG→CGG)  yieE → putative phosphopantetheinyl transferase
RA 3,905,639 (A)9→8 intergenic (‑42/+92) bglF ← / ← bglG beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase/transcriptional antiterminator BglG
RA 3,931,949 A→G T212A (ACG→GCG)  kup → K(+):H(+) symporter Kup
RA 3,944,114 (G)6→7 noncoding (411/2904 nt) rrlC → 23S ribosomal RNA
RA 3,960,276 A→G pseudogene (185/449 nt) yifN ← putative toxin YifN
RA 3,998,655 A→G T225A (ACC→GCC)  uvrD → ssDNA translocase and dsDNA helicase ‑ DNA helicase II
RA 4,002,135 T→C V237A (GTG→GCG)  corA → Ni(2(+))/Co(2(+))/Mg(2(+)) transporter
RA 4,002,463 A→G F113L (TTC→CTC)  yigF ← DUF2628 domain‑containing protein YigF
RA 4,003,610 G→A A190V (GCG→GTG)  rarD ← putative transporter RarD
RA 4,060,280 (T)9→8 intergenic (+50/‑167) bipA → / → yihL ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL
RA 4,143,734 A→G V37V (GTA→GTG frwB → putative PTS enzyme IIB component FrwB
RA 4,186,870 A→G V507V (GTA→GTG rpoC → RNA polymerase subunit beta'
RA 4,189,927 A→G T48A (ACC→GCC)  yjaZ → conserved protein YjaZ
RA 4,198,875 A→T D29V (GAT→GTT)  nfi → endonuclease V
RA 4,206,768 A→G V255V (GTT→GTC purH ← bifunctional AICAR transformylase/IMP cyclohydrolase
RA 4,241,977 A→G V93A (GTC→GCC)  xylE ← D‑xylose:H(+) symporter
RA 4,283,282 A→G F541L (TTT→CTT)  actP ← acetate/glycolate:cation symporter
RA 4,288,518 A→G N252S (AAC→AGC)  nrfA → cytochrome c552 nitrite reductase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,311,353 A→G G230G (GGT→GGC alsB ← D‑allose ABC transporter periplasmic binding protein
RA 4,321,563 C→T G45D (GGC→GAC)  phnG ← carbon‑phosphorus lyase core complex subunit PhnG
RA 4,334,604 A→G C329C (TGT→TGC eptA ← phosphoethanolamine transferase EptA
RA 4,356,045 A→G L123L (TTG→CTG)  dtpC ← dipeptide/tripeptide:H(+) symporter DtpC
RA 4,362,037 A→G intergenic (‑103/+514) cadC ← / ← pheU DNA‑binding transcriptional activator CadC/tRNA‑Phe
RA 4,379,460 C→T G105R (GGA→AGA)  frdC ← fumarate reductase membrane protein FrdC
MC JC 4,396,110 (CTG)4→3 coding (46‑48/1338 nt) amiB → N‑acetylmuramoyl‑L‑alanine amidase B
RA 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA 2'‑O‑ribose G2251 methyltransferase/conserved protein YjfI
RA 4,434,195 A→G H28R (CAC→CGC)  ytfH → putative transcriptional regulator YtfH
RA 4,438,594 (A)6→7 coding (888/939 nt) ytfI → protein YtfI
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,495,766 C→T G148G (GGG→GGA ahr ← aldehyde reductase, NADPH‑dependent
RA 4,502,150 G→A P428S (CCC→TCC)  insG ← KpLE2 phage‑like element; IS4 putative transposase
RA 4,556,769 (T)9→8 coding (116/144 nt) ytiD → protein YtiD
RA 4,580,713 A→G H250H (CAT→CAC hsdS ← type I restriction enzyme EcoKI specificity protein
RA 4,585,687 A→G Y359H (TAT→CAT)  hsdR ← type I restriction enzyme EcoKI endonuclease component
RA 4,593,116 A→G N487S (AAC→AGC)  tsr → methyl‑accepting chemotaxis protein Tsr
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 226393–226968 228563–226971 4–2171 21 [19] [18] 20 rrlH 23S ribosomal RNA
* * ÷ NC_000913 731950 732351–731990 41–402 20 [19] [19] 20 rhsC rhs element protein RhsC
* * ÷ NC_000913 732523–732953 733134–733096 144–612 22 [15] [19] 20 rhsC rhs element protein RhsC
* * ÷ NC_000913 734273–734290 734327 38–55 21 [18] [15] 20 ybfO conserved protein, rhs‑like YbfO
* * ÷ NC_000913 734861–734923 734977–734944 22–117 20 [19] [19] 20 ybfO conserved protein, rhs‑like YbfO
* * ÷ NC_000913 1196277 1211428 15152 24 [19] [18] 21 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1286955 1287090 136 20 [19] [19] 24 [ychS]–[tpr] [ychS],[rttR],[tpr]
* * ÷ NC_000913 2818261 2818491 231 22 [18] [0] 53 [argY]–[argV] [argY],[argV]
* * ÷ NC_000913 3762359–3765288 3765529–3765414 127–3171 20 [18] [17] 24 rhsA rhs element protein RhsA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)88 (0.990) 53/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 281794290 (0.980)37 (0.470) 19/236 2.1 49.0% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 0 (0.000)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 3363001 =166 (1.810)174 (1.920) 73/274 0.1 67.1% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 6 (0.070)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)113 (1.230) 68/276 0.1 93.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 8 (0.090)coding (3907/4134 nt) rhsA rhs element protein RhsA