breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 58,302 T→G intergenic (+123/‑172) djlA → / → yabP co‑chaperone protein DjlA/putative uncharacterized protein YabP
RA 60,250 A→G L33L (TTG→CTG)  rluA ← 23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
RA 76,877 A→G R141R (CGT→CGC sgrR ← DNA‑binding transcriptional dual regulator SgrR
RA 94,350 T→C R395R (CGT→CGC murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 157,738 A→G G463G (GGT→GGC pcnB ← poly(A) polymerase I
RA 169,237 A→G D585G (GAC→GGC)  fhuA → ferrichrome outer membrane transporter/phage receptor
RA 218,835 (A)8→9 intergenic (‑60/+52) proS ← / ← trmO proline‑‑tRNA ligase/tRNA m(6)t(6)A37 methyltransferase
RA 236,132 T→C G22G (GGT→GGC dnaQ → DNA polymerase III subunit epsilon
RA 252,978 T→C R90R (CGT→CGC yafP → putative acyltransferase with acyl‑CoA N‑acyltransferase domain
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 266,477 T→C D26G (GAT→GGT)  yafY ← CP4‑6 prophage; inner membrane lipoprotein YafY
RA 267,569 A→G V146A (GTG→GCG)  yafZ ← CP4‑6 prophage; conserved protein YafZ
RA 276,001 C→T A226V (GCC→GTC)  mmuP → S‑methyl‑L‑methionine transporter
RA 279,941 Δ1 bp intergenic (‑66/+444) insA‑2 ← / ← yagB IS1 protein InsA/orphan antitoxin YagB
RA 293,637 T→C Q94Q (CAA→CAG yagL ← CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL
RA 300,630 C→T G102D (GGC→GAC)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 341,258 A→G N45S (AAC→AGC)  yahJ → putative deaminase with metallo‑dependent hydrolase domain
RA 366,110 C→T A66T (GCA→ACA)  lacZ ← beta‑galactosidase
RA 370,051 A→G T481A (ACC→GCC)  mhpA → putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 390,331 A→G pseudogene (81/1458 nt) yaiT → putative autotransporter YaiT
RA 393,275 T→C pseudogene (306/1449 nt) yaiT → putative autotransporter YaiT
RA 394,868 G→A W121* (TGG→TGA iprA → putative DNA‑binding transcriptional regulator IprA
RA 407,061 A→G Y28C (TAC→TGC)  yaiA → protein YaiA
RA 447,812 T→C T79A (ACG→GCG)  cyoD ← cytochrome bo3 ubiquinol oxidase subunit 4
RA 460,324 A→G A479A (GCA→GCG lon → Lon protease
JC 472,742 (G)6→9 coding (145/339 nt) glnK → nitrogen regulator GlnK
RA 479,422 A→G V166A (GTC→GCC)  maa ← maltose O‑acetyltransferase
RA 524,518 G→T A420S (GCG→TCG)  rhsD → protein RhsD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 535,831 A→G D41G (GAC→GGC)  hyi → hydroxypyruvate isomerase
RA 542,669 T→C L261P (CTC→CCC)  glxK → glycerate 2‑kinase 2
RA 590,918 A→G S13P (TCT→CCT)  nfrA ← bacteriophage N4 receptor, outer membrane protein
RA 596,751 T→C R384R (CGT→CGC cusC → copper/silver export system outer membrane channel
RA 614,854 A→G D233G (GAC→GGC)  entF → apo‑serine activating enzyme
RA 657,119 A→G intergenic (+2/‑173) pagP → / → cspE Lipid IVA palmitoyltransferase/transcription antiterminator and regulator of RNA stability
RA 694,215 A→G D247D (GAT→GAC miaB ← isopentenyl‑adenosine A37 tRNA methylthiolase
RA 699,726 C→T D201N (GAT→AAT)  umpH ← UMP phosphatase
RA 701,932 C→T A274T (GCC→ACC)  nagA ← N‑acetylglucosamine‑6‑phosphate deacetylase
RA 723,528 (T)8→7 coding (887/2685 nt) kdpD ← sensory histidine kinase KdpD
RA 731,035 A→T I485M (ATA→TTG)  rhsC → rhs element protein RhsC
RA 731,037 A→G I485M (ATA→TTG rhsC → rhs element protein RhsC
RA 731,982 C→T G800G (GGC→GGT rhsC → rhs element protein RhsC
RA 731,984 2 bp→AC coding (2402‑2403/4194 nt) rhsC → rhs element protein RhsC
RA 731,989 T→G S803A (TCA→GCA)  rhsC → rhs element protein RhsC
MC JC 770,007 Δ36 bp coding (2030‑2065/2634 nt) mngB → alpha‑mannosidase
RA 773,511 A→G Y157C (TAC→TGC)  cydB → cytochrome bd‑I ubiquinol oxidase subunit II
RA 778,928 A→G M397V (ATG→GTG)  tolB → Tol‑Pal system periplasmic protein TolB
RA 801,849 A→G intergenic (+38/‑38) ybhH → / → ybhI putative isomerase YbhH/putative tricarboxylate transporter
RA 832,792 T→C A53A (GCA→GCG ybiA ← N‑glycosidase YbiA
RA 842,966 A→G D212G (GAT→GGT)  rlmF → 23S rRNA m(6)A1618 methyltransferase
RA 858,019 (G)5→6 coding (1043/1266 nt) ybiU ← DUF1479 domain‑containing protein YbiU
RA 866,548 A→G intergenic (‑184/‑20) moeA ← / → iaaA molybdopterin molybdotransferase/isoaspartyl dipeptidase proenzyme
RA 871,446 C→T G160G (GGC→GGT gsiC → glutathione ABC transporter membrane subunit GsiC
RA 880,940 A→G T72A (ACC→GCC)  dacC → D‑alanyl‑D‑alanine carboxypeptidase DacC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 894,968 A→G intergenic (+72/‑23) potF → / → potG putrescine ABC transporter periplasmic binding protein/putrescine ABC transporter ATP binding subunit
RA 897,059 A→G Q309R (CAG→CGG)  potH → putrescine ABC transporter membrane subunit PotH
RA 902,802 T→C T63A (ACT→GCT)  artI ← putative ABC transporter periplasmic binding protein ArtI
RA 907,602 A→G G227G (GGT→GGC ybjT ← putative NAD(P)‑dependent oxidoreductase YbjT
RA 915,196 T→C intergenic (‑339/+156) lysO ← / ← aqpZ L‑lysine exporter/water channel AqpZ
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 946,269 T→C A133A (GCT→GCC ycaD → putative transporter YcaD
RA 950,923 A→G V53A (GTT→GCT)  pflA ← pyruvate formate‑lyase activating enzyme
RA 995,271 A→G W240R (TGG→CGG)  ssuD ← FMNH2‑dependent alkanesulfonate monooxygenase
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,018,976 (A)8→9 intergenic (+39/+37) matP → / ← ompA macrodomain Ter protein/outer membrane porin A
RA 1,048,907 A→G S142S (AGT→AGC gfcB ← lipoprotein GfcB
RA 1,057,881 T→C E25G (GAG→GGG)  torR ← DNA‑binding transcriptional dual regulator TorR
RA 1,107,290 A→G L312L (CTA→CTG clsC → cardiolipin synthase C
RA 1,113,143 A→G T761A (ACC→GCC)  opgH → osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
RA 1,122,625 C→T intergenic (‑18/+88) pyrC ← / ← yceB dihydroorotase/putative lipid‑binding lipoprotein YceB
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,146,273 A→G L108S (TTG→TCG)  yceF ← m(7)GTP pyrophosphatase
RA 1,157,840 (A)7→6 intergenic (+266/‑29) ycfH → / → ptsG putative metal‑dependent hydrolase YcfH/glucose‑specific PTS enzyme IIBC component
RA 1,187,833 A→G G136G (GGT→GGC roxA ← ribosomal protein‑arginine oxygenase
RA 1,190,955 A→G R344R (CGT→CGC purB ← adenylosuccinate lyase
RA 1,203,536 A→G D90G (GAC→GGC)  ymfL → e14 prophage; uncharacterized protein YmfL
RA 1,207,942 (G)7→8 coding (442/630 nt) ycfK → e14 prophage; protein StfP
RA 1,212,088 (C)8→7 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,215,964 A→G H59R (CAC→CGC)  ycgZ → putative two‑component system connector protein YcgZ
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,221,495 (G)5→6 pseudogene (1895/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,235,072 T→C R45R (CGT→CGC fadR → DNA‑binding transcriptional dual regulator FadR
RA 1,276,245 C→T E458E (GAG→GAA narX ← sensory histidine kinase NarX
RA 1,302,015 G→A L278L (TTG→TTA oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,336,475 C→T T215T (ACC→ACT acnA → aconitate hydratase 1
RA 1,347,071 A→G G614G (GGT→GGC rnb ← RNase II
RA 1,354,843 A→G Y210H (TAT→CAT)  sapB ← putrescine ABC exporter membrane subunit SapB
RA 1,361,982 T→C R24R (CGT→CGC puuR → DNA‑binding transcriptional repressor PuuR
RA 1,396,097 A→G R8G (AGA→GGA)  insH‑4 → IS5 transposase and trans‑activator
RA 1,412,277 G→A P320L (CCC→CTC)  intR ← Rac prophage; putative integrase
RA 1,420,793 (G)6→5 coding (110/423 nt) ydaT → Rac prophage; protein YdaT
RA 1,447,390 C→T intergenic (‑107/‑129) feaR ← / → feaB DNA‑binding transcriptional activator FeaR/phenylacetaldehyde dehydrogenase
RA 1,456,663 A→G G78G (GGA→GGG paaE → phenylacetyl‑CoA 1,2‑epoxidase, reductase subunit
RA 1,458,625 (G)6→7 coding (362/789 nt) paaG → putative ring 1,2‑epoxyphenylacetyl‑CoA isomerase (oxepin‑CoA forming)
RA 1,499,780 C→T A224T (GCC→ACC)  ydcK ← putative enzyme YdcK
RA 1,515,125 A→G T122A (ACC→GCC)  ydcV → putative ABC transporter membrane subunit YdcV
JC 1,533,857 +TATC intergenic (‑6/‑167) yddH ← / → nhoA flavin reductase‑like protein YddH/arylamine N‑acetyltransferase
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,569,312 A→G V393A (GTT→GCT)  gadC ← L‑glutamate:4‑aminobutyrate antiporter
RA 1,584,678 +A coding (1809/2280 nt) ydeP ← putative oxidoreductase YdeP
RA 1,594,132 (C)8→7 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,594,633 T→C pseudogene (3454/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,597,313 (C)7→8 pseudogene (774/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,632,770 T→C intergenic (‑485/‑120) ydfJ ← / → ynfT putative transporter YdfJ/Qin prophage; protein YnfT
RA 1,632,837 A→G intergenic (‑552/‑53) ydfJ ← / → ynfT putative transporter YdfJ/Qin prophage; protein YnfT
RA 1,632,949 C→T A20A (GCC→GCT ynfT → Qin prophage; protein YnfT
RA 1,639,896 A→G G54G (GGT→GGC rrrQ ← Qin prophage; putative lysozyme
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,694,138 A→G G42G (GGT→GGC uidB ← glucuronide:H(+) symporter
RA 1,709,415 (C)7→9 coding (274/1059 nt) rsxD → SoxR [2Fe‑2S] reducing system protein RsxD
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,721,159 T→C R45R (CGT→CGC ydhI → DUF1656 domain‑containing protein YdhI
RA 1,727,494 (G)6→8 coding (836/1098 nt) nemA → N‑ethylmaleimide reductase
RA 1,730,557 C→T P491S (CCG→TCG)  lhr → putative ATP‑dependent helicase Lhr
RA 1,742,242 T→C V277A (GTT→GCT)  cfa → cyclopropane fatty acyl phospholipid synthase
RA 1,784,262 G→A G429D (GGC→GAC)  fadK → short chain acyl‑CoA synthetase
RA 1,813,656 T→C K3R (AAA→AGA)  cedA ← cell division modulator
RA 1,866,080 A→G D131D (GAT→GAC mipA ← scaffolding protein that interacts with murein polymerase and murein hydrolase
RA 1,871,508 G→A W375* (TGG→TAG)  cdgI → putative c‑di‑GMP binding protein CdgI
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,903,423 T→C R114R (CGT→CGC manY → mannose‑specific PTS enzyme IIC component
RA 1,904,364 T→C V157A (GTG→GCG)  manZ → mannose‑specific PTS enzyme IID component
RA 1,944,255 T→C intergenic (+56/+91) znuB → / ← ruvB Zn(2(+)) ABC transporter membrane subunit/Holliday junction branch migration complex subunit RuvB
RA 1,974,645 T→C E227G (GAA→GGA)  cheA ← chemotaxis protein CheA
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,988,106 (C)6→7 intergenic (‑147/‑116) azuC ← / → yecR uncharacterized protein AzuC/lipoprotein YecR
RA 1,993,987 G→A T240T (ACC→ACT uvrC ← excision nuclease subunit C
RA 1,995,334 T→C D9G (GAC→GGC)  uvrY ← DNA‑binding transcriptional activator UvrY
RA 2,073,574 A→G N679S (AAC→AGC)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,137,892 Δ1 bp intergenic (‑649/‑10) wza ← / → yegH outer membrane polysaccharide export protein Wza/inner membrane protein YegH
RA 2,167,478 T→C T15A (ACA→GCA)  ogrK ← prophage P2 late control protein OgrK
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,194,993 A→G I232V (ATC→GTC)  metG → methionine‑‑tRNA ligase
RA 2,230,727 C→T T35I (ACC→ATC)  yohJ → PF03788 family membrane protein YohJ
RA 2,258,737 T→C N187S (AAC→AGC)  psuK ← putative pseudouridine kinase
RA 2,281,874 T→C F415L (TTT→CTT)  radD → putative DNA repair helicase RadD
RA 2,299,683 (T)5→4 coding (578/696 nt) napG ← ferredoxin‑type protein NapG
RA 2,305,810 T→C K315K (AAA→AAG mqo ← malate:quinone oxidoreductase
RA 2,307,612 T→G N335T (AAC→ACC)  yojI ← ABC transporter family protein/microcin J25 efflux protein
RA 2,320,303 (C)5→4 coding (261/1827 nt) atoS → sensory histidine kinase AtoS
RA 2,368,443 G→A M135I (ATG→ATA arnA → fused UDP‑4‑amino‑4‑deoxy‑L‑arabinose formyltransferase/UDP‑glucuronate dehydrogenase
RA 2,373,161 T→C A90A (GCT→GCC arnF → undecaprenyl‑phosphate‑alpha‑L‑Ara4N flippase ‑ ArnF subunit
RA 2,381,909 T→C V101A (GTG→GCG)  rbn → ribonuclease BN
RA 2,400,895 T→C T221A (ACC→GCC)  nuoF ← NADH:quinone oxidoreductase subunit F
RA 2,405,417 (C)6→7 intergenic (‑345/+286) nuoA ← / ← lrhA NADH:quinone oxidoreductase subunit A/DNA‑binding transcriptional dual regulator LrhA
RA 2,425,237 T→C D227G (GAC→GGC)  hisQ ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ
RA 2,426,785 (T)6→7 coding (4/783 nt) hisJ ← histidine ABC transporter periplasmic binding protein
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,472,070 T→C intergenic (‑8/+42) yfdL ← / ← yfdM CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL/CPS‑53 (KpLE1) prophage; putative methyltransferase
RA 2,474,455 T→C W142R (TGG→CGG)  yfdQ → CPS‑53 (KpLE1) prophage; protein YfdQ
RA 2,478,169 T→C V108A (GTT→GCT)  dsdX → D‑serine transporter
RA 2,518,470 A→G T2A (ACA→GCA)  yfeC → putative DNA‑binding transcriptional regulator YfeC
RA 2,526,791 A→G R22R (CGT→CGC yfeR ← putative LysR‑type DNA‑binding transcriptional regulator YfeR
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,552,141 T→C intergenic (‑3/‑211) ypeA ← / → amiA putative acetyltransferase YpeA/N‑acetylmuramoyl‑L‑alanine amidase A
RA 2,552,558 T→C D69D (GAT→GAC amiA → N‑acetylmuramoyl‑L‑alanine amidase A
RA 2,564,534 A→G E4E (GAA→GAG yffS → CPZ‑55 prophage; uncharacterized protein YffS
RA 2,565,560 T→C D442G (GAC→GGC)  eutA ← reactivating factor for ethanolamine ammonia‑lyase EutBC
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,604,561 G→A A239T (GCA→ACA)  hyfC → hydrogenase 4 component C
RA 2,626,471 A→G T453A (ACC→GCC)  ppx → exopolyphosphatase
RA 2,646,583 (G)6→5 coding (743/2313 nt) pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,725,721 T→C D9G (GAC→GGC)  kgtP ← alpha‑ketoglutarate:H(+) symporter
RA 2,734,543 (C)8→9 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,744,611 T→C E243E (GAA→GAG trmD ← tRNA m(1)G37 methyltransferase
RA 2,747,069 T→C T243A (ACC→GCC)  ffh ← signal recognition particle protein component
RA 2,754,863 T→C intergenic (‑99/‑33) ratA ← / → smpB ribosome association toxin RatA/SsrA‑binding protein
RA 2,768,347 G→A C213Y (TGT→TAT)  yfjP → CP4‑57 prophage; putative GTP‑binding protein YfjP
RA 2,786,623 (A)6→5 pseudogene (227/2253 nt) ygaQ → putative uncharacterized protein YgaQ
RA 2,790,141 G→A V54I (GTC→ATC)  lhgO → L‑2‑hydroxyglutarate oxidase
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,844,595 T→C N53S (AAC→AGC)  hycF ← formate hydrogenlyase subunit HycF
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,861,229 T→C I2M (ATA→ATG ygbI ← putative DeoR‑type DNA‑binding transcriptional regulator YgbI
RA 2,863,460 T→C F376L (TTT→CTT)  ygbK → putative 3‑oxo‑tetronate kinase YgbK
RA 2,890,515 T→C T462A (ACC→GCC)  cysJ ← sulfite reductase, flavoprotein subunit
RA 2,894,436 A→G T81A (ACC→GCC)  ygcP → putative anti‑terminator regulatory protein
RA 2,911,200 A→G V47A (GTG→GCG)  mazE ← antitoxin of the MazF‑MazE toxin‑antitoxin system MazE
RA 2,935,949 A→G A122A (GCA→GCG fucI → L‑fucose isomerase
RA 2,949,236 T→C intergenic (‑226/‑6) amiC ← / → argA N‑acetylmuramoyl‑L‑alanine amidase C/N‑acetylglutamate synthase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,953,839 G→A A722V (GCC→GTC)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,966,109 G→A intergenic (‑72/+79) lgt ← / ← ptsP phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase/phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 2,968,718 T→C D87G (GAC→GGC)  rppH ← RNA pyrophosphohydrolase
RA 2,988,019 T→C Y162H (TAT→CAT)  yqeH → putative LuxR family transcriptional regulator YqeH
RA 2,999,680 (G)7→8 coding (212/756 nt) ygeR ← LysM domain‑containing putative peptidase lipoprotein YgeR
RA 3,006,352 A→G T31A (ACG→GCG)  ygeW → putative carbamoyltransferase YgeW
RA 3,016,733 T→C L225P (CTC→CCC)  ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,021,444 Δ1 bp coding (129/2871 nt) xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,031,583 Δ1 bp coding (217/1449 nt) uacT → urate:H(+) symporter
RA 3,033,157 A→G L31L (TTA→TTG idi → isopentenyl‑diphosphate Delta‑isomerase
RA 3,045,619 T→C T95A (ACC→GCC)  ygfF ← putative NAD(P)‑binding oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,081,892 A→G intergenic (‑257/‑21) tktA ← / → loiP transketolase 1/metalloprotease LoiP
RA 3,093,919 C→T intergenic (+3/+181) yqgF → / ← yggR ribonuclease H‑like domain containing nuclease/Type II/IV secretion system family protein
RA 3,095,346 C→T G83G (GGC→GGT yggS → PLP homeostasis protein
RA 3,101,067 T→C N186S (AAC→AGC)  yggN ← putative EcfF
RA 3,109,075 A→G D27D (GAT→GAC speC ← ornithine decarboxylase, biosynthetic
RA 3,119,973 G→A S436F (TCC→TTC)  glcA ← glycolate/lactate:H(+) symporter GlcA
RA 3,139,399 T→C Y225H (TAT→CAT)  yghU → disulfide reductase/organic hydroperoxide reductase
RA 3,148,428 (A)6→7 coding (532/1041 nt) gpr → L‑glyceraldehyde 3‑phosphate reductase
RA 3,153,839 T→C L93L (TTA→CTA)  yghB → DedA family protein YghB
RA 3,157,712 (C)6→7 coding (63/927 nt) yqhG → DUF3828 domain‑containing protein YqhG
RA 3,165,384 T→C D197G (GAC→GGC)  parC ← DNA topoisomerase IV subunit A
RA 3,180,279 T→C V179A (GTA→GCA)  ygiB → DUF1190 domain‑containing protein YgiB
RA 3,181,931 C→T M168I (ATG→ATA ygiD ← 4,5‑DOPA dioxygenase extradiol
RA 3,203,779 T→C N118S (AAC→AGC)  bacA ← undecaprenyl pyrophosphate phosphatase
RA 3,210,821 T→C V14A (GTA→GCA)  rpsU → 30S ribosomal subunit protein S21
RA 3,238,460 A→G intergenic (+163/‑120) ygjR → / → alx putative oxidoreductase YgjR/putative membrane‑bound redox modulator Alx
RA 3,245,550 T→C G109G (GGT→GGC exuT → hexuronate transporter
RA 3,270,488 (C)7→9 noncoding (105/377 nt) rnpB ← RNase P catalytic RNA component
RA 3,286,059 T→C H194H (CAT→CAC agaD → galactosamine‑specific PTS enzyme IID component
RA 3,291,477 A→G N46S (AAC→AGC)  yraK → putative fimbrial adhesin YraK
RA 3,301,885 A→G D134G (GAC→GGC)  yhbU → putative peptidase YhbU
RA 3,337,780 T→C T38A (ACG→GCG)  mlaC ← intermembrane phospholipid transport system ‑ periplasmic binding protein
RA 3,344,042 T→C G33G (GGT→GGC lptB → lipopolysaccharide transport system ATP binding protein
RA 3,348,053 T→C F30L (TTT→CTT)  npr → phosphorelay protein Npr
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,373,324 T→C T85A (ACC→GCC)  nanA ← N‑acetylneuraminate lyase
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,400,773 A→G R105R (CGT→CGC mreB ← dynamic cytoskeletal protein MreB
RA 3,413,816 T→C intergenic (‑351/‑48) envR ← / → acrE DNA‑binding transcriptional repressor EnvR/multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,424,645 A→G noncoding (2139/2904 nt) rrlD ← 23S ribosomal RNA
RA 3,426,921 A→G intergenic (‑138/+37) rrlD ← / ← alaU 23S ribosomal RNA/tRNA‑Ala
RA 3,428,808 T→C intergenic (‑46/‑428) rrsD ← / → yrdA 16S ribosomal RNA/protein YrdA
RA 3,467,767 T→C D696G (GAC→GGC)  chiA ← endochitinase
RA 3,475,019 G→A A105V (GCC→GTC)  tusC ← sulfurtransferase complex subunit TusC
RA 3,537,315 G→A intergenic (+27/‑70) greB → / → yhgF transcription elongation factor GreB/putative RNA‑binding protein YhgF
RA 3,537,763 G→A A127T (GCT→ACT)  yhgF → putative RNA‑binding protein YhgF
RA 3,541,770 T→C V455A (GTC→GCC)  feoB → Fe(2(+)) transporter FeoB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,576,646 T→C N139S (AAC→AGC)  gntU ← low‑affinity gluconate transporter
RA 3,625,168 T→C V264A (GTC→GCC)  yhhI → putative transposase
RA 3,631,981 T→C T204A (ACC→GCC)  yhiJ ← DUF4049 domain‑containing protein YhiJ
RA 3,650,871 T→C intergenic (+209/‑420) arsC → / → yhiS arsenate reductase/putative uncharacterized protein YhiS
RA 3,668,544 (C)6→7 coding (645/1398 nt) ccp ← cytochrome c peroxidase
RA 3,676,025 G→A V414M (GTG→ATG)  yhjE → putative transporter YhjE
RA 3,683,474 T→C intergenic (‑27/+156) dctA ← / ← pdeK C4 dicarboxylate/orotate:H(+) symporter/putative c‑di‑GMP phosphodiesterase PdeK
RA 3,692,951 Δ1 bp coding (2286/2619 nt) bcsA ← cellulose synthase catalytic subunit
RA 3,714,076 T→C V142A (GTG→GCG) 
P773P (CCA→CCG
yiaC →
bisC ←
putative acyltransferase with acyl‑CoA N‑acyltransferase domain
biotin sulfoxide reductase
RA 3,735,669 G→A A231T (GCC→ACC)  xylR → DNA‑binding transcriptional dual regulator XylR
RA 3,739,696 T→C intergenic (+169/‑9) malS → / → avtA alpha‑amylase/valine‑‑pyruvate aminotransferase
RA 3,772,697 G→A G139G (GGG→GGA mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,812,130 T→C T144A (ACG→GCG)  yicR ← RadC‑like JAB domain‑containing protein YicR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,818,975 T→C I34I (ATT→ATC yicG → conserved inner membrane protein YicG
RA 3,834,428 (G)6→5 coding (110/2319 nt) yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,849,433 G→A T235M (ACG→ATG)  uhpB ← sensory histidine kinase UhpB
RA 3,852,208 C→T A193T (GCC→ACC)  ilvB ← acetohydroxy acid synthase I subunit IlvB
RA 3,854,090 G→A G57S (GGT→AGT)  emrD → multidrug efflux pump EmrD
RA 3,856,753 (T)5→6 coding (339/450 nt) yidI → putative inner membrane protein
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,864,868 T→C Y469C (TAT→TGT)  yidE ← putative transport protein YidE
RA 3,885,636 G→A P187P (CCG→CCA yidC → membrane protein insertase YidC
RA 3,890,443 G→A G70S (GGC→AGC)  tnaB → tryptophan:H(+) symporter TnaB
RA 3,898,816 A→C V193G (GTC→GGC)  yieK ← putative glucosamine‑6‑phosphate deaminase YieK
RA 3,922,549 (C)5→4 intergenic (‑109/+508) atpI ← / ← rsmG ATP synthase accessory factor/16S rRNA m(7)G527 methyltransferase
RA 3,925,336 C→G D100H (GAT→CAT)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 3,931,183 (G)7→8 intergenic (‑90/‑133) ravA ← / → kup regulatory ATPase RavA/K(+):H(+) symporter Kup
RA 3,949,304 A→G G222G (GGT→GGC yifB ← putative magnesium chelatase YifB
RA 3,953,356 C→T G291G (GGC→GGT ilvE → branched‑chain‑amino‑acid aminotransferase
RA 3,956,738 A→G T470A (ACC→GCC)  ilvA → threonine deaminase
RA 3,976,956 A→G T227A (ACG→GCG)  wzxE → lipid III flippase
RA 3,999,720 T→C F580L (TTC→CTC)  uvrD → ssDNA translocase and dsDNA helicase ‑ DNA helicase II
RA 4,007,246 C→T G461G (GGC→GGT recQ → ATP‑dependent DNA helicase RecQ
RA 4,010,309 A→G N37S (AAC→AGC)  yigL → phosphosugar phosphatase YigL
RA 4,032,000 A→G E280E (GAA→GAG pepQ → Xaa‑Pro dipeptidase
RA 4,053,014 A→G M324V (ATG→GTG)  hemN → coproporphyrinogen III dehydrogenase
RA 4,062,831 A→G A195A (GCA→GCG yihN → putative transporter YihN
RA 4,104,208 A→G R256R (CGT→CGC cpxA ← sensory histidine kinase CpxA
RA 4,126,222 A→G L197L (TTG→CTG)  priA ← primosome factor N'
RA 4,151,270 A→G V610A (GTA→GCA)  ppc ← phosphoenolpyruvate carboxylase
RA 4,156,584 A→G Q191R (CAA→CGA)  argB → acetylglutamate kinase
RA 4,181,903 T→C I220T (ATC→ACC)  rpoB → RNA polymerase subunit beta
RA 4,233,858 A→G D34G (GAC→GGC)  pgi → glucose‑6‑phosphate isomerase
RA 4,241,484 T→C K257K (AAA→AAG xylE ← D‑xylose:H(+) symporter
RA 4,267,558 C→T P149S (CCC→TCC)  tyrB → tyrosine aminotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,302,825 A→G V85A (GTG→GCG)  mdtO ← putative multidrug efflux pump subunit MdtO
JC JC 4,306,431 IS1 (+) +9 bp coding (159‑167/1986 nt) yjcS ← alkyl sulfatase
RA 4,429,416 C→T A161V (GCA→GTA)  fklB → FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB
RA 4,441,848 T→C Q110R (CAG→CGG)  msrA ← methionine sulfoxide reductase A
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,451,045 A→G intergenic (+127/‑13) ytfQ → / → ytfR galactofuranose ABC transporter periplasmic binding protein/galactofuranose ABC transporter putative ATP binding subunit
RA 4,454,129 A→G T183A (ACC→GCC)  yjfF → galactofuranose ABC transporter putative membrane subunit YjtF
RA 4,468,257 G→A S211S (TCG→TCA mgtA → Mg(2(+)) importing P‑type ATPase
RA 4,474,275 (C)6→7 coding (152/594 nt) bdcR → putative transcriptional regulator BdcR
RA 4,488,579 (C)7→8 coding (1485/1503 nt) yjgR ← DUF853 domain‑containing protein YjgR
RA 4,493,414 A→G G331G (GGT→GGC idnD ← L‑idonate 5‑dehydrogenase
RA 4,504,246 A→G R63R (AGA→AGG yjhB → putative sialic acid transporter
RA 4,520,902 A→G G373G (GGT→GGC yjhF ← KpLE2 phage‑like element; putative transporter YjhF
RA 4,528,706 A→G V71A (GTG→GCG)  sgcQ ← KpLE2 phage‑like element; putative nucleoside triphosphatase
RA 4,565,988 A→G L420S (TTG→TCG)  yjiN ← DUF445 domain‑containing protein YjiN
RA 4,578,548 G→A Q264* (CAG→TAG)  mcrB ← 5‑methylcytosine‑specific restriction enzyme subunit McrB
RA 4,586,655 A→G L36P (CTG→CCG)  hsdR ← type I restriction enzyme EcoKI endonuclease component
RA 4,590,443 T→C A279A (GCA→GCG btsT ← pyruvate:H(+) symporter
RA 4,593,296 A→G N547S (AAC→AGC)  tsr → methyl‑accepting chemotaxis protein Tsr
RA 4,604,288 T→C S43S (AGT→AGC bglJ → DNA‑binding transcriptional regulator BglJ
JC 4,606,215 (G)8→11 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,329 (G)8→6 noncoding (72‑73/87 nt) leuQ ← tRNA‑Leu
RA 4,611,965 T→C G190G (GGT→GGC osmY → periplasmic chaperone OsmY
RA 4,629,935 T→C E196E (GAA→GAG ettA ← energy‑dependent translational throttle protein EttA
RA 4,630,384 T→C T47A (ACC→GCC)  ettA ← energy‑dependent translational throttle protein EttA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 270640–271482 271482 1–843 40 [35] [33] 40 insI‑1 IS30 transposase
* * ÷ NC_000913 731427–731470 731586–731521 52–160 38 [35] [32] 37 rhsC rhs element protein RhsC
* * ÷ NC_000913 732702–732959 734610–734476 1518–1909 37 [35] [35] 37 [rhsC]–[ybfO] [rhsC],ybfB,[ybfO]
* * ÷ NC_000913 1269855–1269921 1270363–1270091 171–509 36 [35] [35] 36 rdlB–ldrC rdlB,ldrC
* * ÷ NC_000913 1286784 1286915–1286885 102–132 37 [35] [35] 36 ychS putative uncharacterized protein YchS
* * ÷ NC_000913 3423762–3424234 3424525–3424238 5–764 36 [35] [29] 37 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =11 (0.070)145 (1.110) 73/244 0.2 91.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 18 (0.140)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780511 (0.070)83 (0.640) 52/244 0.7 85.7% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 18 (0.140)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)204 (1.420) 97/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =305 (2.070)338 (2.310) 99/274 0.0 68.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 8 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)194 (1.310) 90/276 0.1 94.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 11 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA