breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 34,839 T→C Q178R (CAA→CGA)  caiE ← putative transferase CaiE
RA 67,549 C→T M263I (ATG→ATA araA ← L‑arabinose isomerase
RA 68,506 C→T A515T (GCC→ACC)  araB ← ribulokinase
RA 72,878 C→T R17H (CGT→CAT)  thiQ ← thiamine ABC transporter ATP binding subunit
RA 81,515 C→T Q148Q (CAG→CAA leuB ← 3‑isopropylmalate dehydrogenase
RA 98,181 A→G V365V (GTA→GTG murD → UDP‑N‑acetylmuramoyl‑L‑alanine‑‑D‑glutamate ligase
RA 122,170 A→G T27A (ACT→GCT)  pdhR → DNA‑binding transcriptional dual regulator PdhR
RA 144,822 A→G K82K (AAA→AAG yadI → putative PTS enzyme IIA component YadI
RA 149,567 T→C Y12C (TAC→TGC)  panB ← 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
RA 167,602 A→G D40G (GAC→GGC)  fhuA → ferrichrome outer membrane transporter/phage receptor
RA 200,200 C→T P758L (CCG→CTG)  bamA → outer membrane protein assembly factor BamA
RA 211,877 A→G M1M (ATG→GTG) † yaeR → VOC domain‑containing protein YaeR
RA 215,141 (A)8→7 coding (309/423 nt) arfB → peptidyl‑tRNA hydrolase, ribosome rescue factor
RA 242,717 A→T I196N (ATC→AAC)  fadE ← acyl‑CoA dehydrogenase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 263,909 C→T R33R (CGG→CGA yafW ← antitoxin of the YkfI‑YafW toxin‑antitoxin pair
RA 267,042 A→G intergenic (‑489/+142) yafY ← / ← yafZ CP4‑6 prophage; inner membrane lipoprotein YafY/CP4‑6 prophage; conserved protein YafZ
RA 298,172 A→G R185R (CGT→CGC paoD ← molybdenum cofactor insertion chaperone for PaoABC
RA 380,021 (G)10→8 intergenic (‑140/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 397,915 T→C V15A (GTT→GCT)  yaiW → surface‑exposed outer membrane lipoprotein
RA 404,305 A→G E201E (GAA→GAG dgcC → diguanylate cyclase DgcC
RA 406,193 T→C W144R (TGG→CGG)  yaiI → DUF188 domain‑containing protein YaiI
RA 442,234 A→G N229S (AAC→AGC)  thiI → tRNA uridine 4‑sulfurtransferase
RA 445,815 G→A C284C (TGC→TGT yajR ← putative major facilitator superfamily transport protein YajR
RA 471,607 A→G G324G (GGA→GGG mdlB → ABC transporter family protein MdlB
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 550,199 T→C G191G (GGT→GGC ylbF → DUF2877 domain‑containing protein YlbF
MC JC 551,375 Δ70 bp intergenic (+43/+83) ybcF → / ← purK putative carbamate kinase/5‑(carboxyamino)imidazole ribonucleotide synthase
RA 556,489 A→G V22A (GTC→GCC)  ybcI ← conserved inner membrane protein YbcI
RA 566,871 (A)6→5 coding (30/300 nt) insE‑3 → DLP12 prophage; IS3 element protein InsE
RA 579,108 A→G intergenic (‑215/+76) borD ← / ← ybcV DLP12 prophage; prophage lipoprotein BorD/DLP12 prophage; protein YbcV
RA 637,019 T→C Q200Q (CAA→CAG ybdO ← putative LysR family DNA‑binding transcriptional regulator YbdO
RA 640,119 A→G T123A (ACC→GCC)  ahpF → alkyl hydroperoxide reductase, AhpF component
RA 645,711 C→T L290L (CTG→CTA citT ← citrate:succinate antiporter
RA 646,353 A→G D76D (GAT→GAC citT ← citrate:succinate antiporter
RA 647,203 A→G F103L (TTC→CTC)  citG ← triphosphoribosyl‑dephospho‑CoA synthase
RA 667,244 A→G V325A (GTT→GCT)  mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 686,826 +C intergenic (‑157/+13) gltJ ← / ← gltI glutamate/aspartate ABC transporter membrane subunit GltJ/glutamate/aspartate ABC transporter periplasmic binding protein
RA 687,043 A→G G235G (GGT→GGC gltI ← glutamate/aspartate ABC transporter periplasmic binding protein
RA 693,311 C→G G23A (GGC→GCC)  ybeZ ← PhoH‑like protein
RA 710,089 G→A W100* (TGG→TGA chiQ → lipoprotein ChiQ
RA 768,123 A→G Y49C (TAT→TGT)  mngB → alpha‑mannosidase
RA 783,280 C→T L39F (CTT→TTT)  pnuC → nicotinamide riboside transporter
RA 864,274 G→A W211* (TGG→TGA fsaA → fructose‑6‑phosphate aldolase 1
JC 868,422 (C)8→11 coding (903/1872 nt) gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 921,427 A→G N361D (AAC→GAC)  macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 938,898 A→G D302G (GAC→GGC)  rarA → recombination factor
RA 942,977 T→A D673E (GAT→GAA dmsA → dimethyl sulfoxide reductase subunit A
RA 948,681 A→G S296S (AGT→AGC ycaN ← putative LysR‑type transcriptional regulator YcaN
RA 961,535 C→T A112V (GCA→GTA)  cmk → cytidylate kinase
RA 1,002,224 (G)7→8 coding (418/1071 nt) elfG → putative fimbrial‑like adhesin protein
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,062,228 A→G T144A (ACC→GCC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
RA 1,062,547 A→G intergenic (+149/+3) torD → / ← cbpM trimethylamine‑N‑oxide reductase‑specific chaperone/chaperone modulator CbpM
RA 1,108,782 A→G F54L (TTC→CTC)  opgC ← protein required for succinyl modification of osmoregulated periplasmic glucans
RA 1,135,901 Δ1 bp coding (338/699 nt) flgH → flagellar L‑ring protein
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,240,582 C→T G497D (GGT→GAT)  cvrA ← putative K(+):H(+) antiporter
RA 1,257,583 A→G V77A (GTA→GCA)  ychF ← redox‑responsive ATPase YchF
RA 1,260,656 A→G L50L (TTG→CTG)  dauA ← aerobic C4‑dicarboxylate transporter DauA
RA 1,264,036 A→G D108G (GAT→GGT)  hemA → glutamyl‑tRNA reductase
RA 1,270,445 T→C noncoding (53/68 nt) rdlC → small regulatory antisense RNA RdlC
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,308,423 A→G W75R (TGG→CGG)  clsA ← cardiolipin synthase A
RA 1,344,172 T→C S59G (AGT→GGT)  yciT ← putative DNA‑binding transcriptional regulator YciT
RA 1,359,363 T→C intergenic (‑22/+127) ymjE ← / ← puuA protein YmjE/glutamate‑putrescine ligase
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
RA 1,399,667 T→C intergenic (‑141/+54) fnr ← / ← ogt DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,405,021 C→T A94V (GCA→GTA)  abgR → putative LysR‑type DNA‑binding transcriptional regulator AbgR
RA 1,405,078 T→C V113A (GTC→GCC)  abgR → putative LysR‑type DNA‑binding transcriptional regulator AbgR
RA 1,442,261 A→G F195L (TTC→CTC)  ldhA ← D‑lactate dehydrogenase
RA 1,475,227 T→C C28C (TGT→TGC ydbD → DUF2773 domain‑containing protein YdbD
RA 1,505,308 Δ1 bp coding (773/1176 nt) ydcO ← putative transport protein YdcO
RA 1,510,788 (A)8→7 coding (786/1407 nt) ydcR → fused putative DNA‑binding transcriptional regulator/putative aminotransferase YdcR
RA 1,511,968 A→G K105K (AAA→AAG ydcS → putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
RA 1,521,361 T→C T569A (ACC→GCC)  yncD ← putative TonB‑dependent outer membrane receptor
RA 1,538,868 T→C V1241V (GTA→GTG narZ ← nitrate reductase Z subunit alpha
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,577,857 C→T A496T (GCC→ACC)  yddA ← ABC transporter family protein YddA
RA 1,582,309 T→C Q72Q (CAA→CAG ydeN ← putative sulfatase
RA 1,594,132 (C)8→9 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,594,256 (C)8→9 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,597,313 (C)7→6 pseudogene (774/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,606,796 C→T R233C (CGC→TGC)  lsrF → 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
RA 1,606,917 T→C V273A (GTT→GCT)  lsrF → 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
RA 1,612,010 C→T A106T (GCG→ACG)  yneG ← conserved protein YneG
RA 1,618,524 T→C T129A (ACT→GCT)  marC ← inner membrane protein MarC
RA 1,636,290 T→C G26G (GGA→GGG nohA ← Qin prophage; putative prophage DNA‑packaging protein NohA
RA 1,668,069 G→A R166C (CGC→TGC)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,670,408 A→G D237G (GAC→GGC)  ynfM → putative transporter YnfM
RA 1,680,973 A→G intergenic (+34/‑3) ydgI → / → folM putative arginine:ornithine antiporter/dihydromonapterin reductase
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,711,998 T→C V159A (GTA→GCA)  nth → endonuclease III
RA 1,776,375 A→G A263A (GCA→GCG ydiF → putative acetate‑CoA transferase
RA 1,792,782 T→C intergenic (‑51/+27) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
RA 1,793,863 A→G C83R (TGT→CGT)  btuE ← thioredoxin/glutathione peroxidase
RA 1,865,335 C→T R101H (CGT→CAT)  yeaE ← methylglyoxal reductase YeaE
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,879,362 (T)8→7 intergenic (‑107/+41) yeaQ ← / ← yoaG PF04226 family protein YeaQ/DUF1869 domain‑containing protein YoaG
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,717 T→C L5L (TTA→TTG yoaH ← UFP0181 family protein YoaH
RA 1,899,621 G→A V399I (GTT→ATT)  pdeD → putative c‑di‑GMP phosphodiesterase PdeD
RA 1,901,309 (C)6→7 coding (277/1557 nt) yoaE ← putative inner membrane protein
RA 1,906,464 T→C A199A (GCA→GCG rlmA ← 23S rRNA m(1)G745 methyltransferase
RA 1,921,182 (C)5→6 coding (960/1440 nt) rsmF → 16S rRNA m(5)C1407 methyltransferase
RA 1,923,946 (G)6→7 coding (646/873 nt) yebZ ← putative inner membrane protein
RA 1,928,727 T→A D38V (GAC→GTC)  ptrB ← oligopeptidase B
RA 1,934,624 T→C intergenic (‑20/+215) edd ← / ← zwf phosphogluconate dehydratase/NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
RA 1,936,923 T→C V91A (GTT→GCT)  yebK → DNA‑binding transcriptional repressor YebK
RA 1,965,120 G→A Q358* (CAG→TAG)  flhB ← flagellar biosynthesis protein FlhB
RA 1,967,179 T→C T87A (ACT→GCT)  cheY ← chemotaxis protein CheY
RA 1,967,855 T→C D216G (GAC→GGC)  cheB ← protein‑glutamate methylesterase/protein glutamine deamidase
RA 1,974,881 T→C E148E (GAA→GAG cheA ← chemotaxis protein CheA
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,996,183 T→C N217S (AAC→AGC)  sdiA ← DNA‑binding transcriptional dual regulator SdiA
RA 1,998,555 G→A P309L (CCG→CTG)  dcyD ← D‑cysteine desulfhydrase
RA 2,016,404 T→C A179A (GCT→GCC fliH → flagellar biosynthesis protein FliH
RA 2,031,164 C→G G385A (GGT→GCT)  dcm ← DNA‑cytosine methyltransferase
RA 2,033,739 (T)8→9 noncoding (91/91 nt) rseX → small regulatory RNA RseX
RA 2,072,948 T→C D470D (GAT→GAC flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,100,810 T→C A153A (GCA→GCG gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating
RA 2,120,995 T→C N187S (AAC→AGC)  wcaJ ← undecaprenyl‑phosphate glucose phosphotransferase
RA 2,133,799 T→C E618E (GAA→GAG wzc ← protein‑tyrosine kinase Wzc
RA 2,165,573 (C)6→7 intergenic (+52/‑95) yegP → / → yegQ DUF1508 domain‑containing protein YegP/putative peptidase YegQ
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,180,672 T→C V193A (GTT→GCT)  yegU → putative ADP‑ribosylglycohydrolase YegU
RA 2,192,815 T→C intergenic (‑19/+244) yehE ← / ← mrp DUF2574 domain‑containing protein YehE/P‑loop NTPase family protein
RA 2,193,578 T→C G197G (GGA→GGG mrp ← P‑loop NTPase family protein
RA 2,202,629 (G)6→7 coding (2351/3633 nt) yehI → DUF4132 domain‑containing protein YehI
RA 2,202,630 A→G G784G (GGA→GGG yehI → DUF4132 domain‑containing protein YehI
RA 2,210,473 (C)6→7 coding (1374/1845 nt) yehQ → SWIM zinc finger domains‑containing protein YehQ
RA 2,251,286 T→C G149G (GGT→GGC nfo → endonuclease IV
RA 2,259,447 G→A intergenic (‑151/+272) psuK ← / ← fruA putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,295,780 T→C N181D (AAC→GAC)  ccmC ← heme trafficking system membrane protein CcmC
RA 2,312,660 G→A N30N (AAC→AAT ompC ← outer membrane porin C
RA 2,317,538 T→C E780G (GAG→GGG)  rcsC ← sensory histidine kinase RcsC
RA 2,324,731 A→T D208V (GAT→GTT)  atoA → acetyl‑CoA:acetoacetyl‑CoA transferase subunit beta
RA 2,347,460 T→C V26A (GTG→GCG)  nrdB → ribonucleoside‑diphosphate reductase 1 subunit beta
RA 2,359,279 T→C T292A (ACC→GCC)  yfaV ← putative transporter YfaV
RA 2,376,098 T→C D241G (GAC→GGC)  menB ← 1,4‑dihydroxy‑2‑naphthoyl‑CoA synthase
RA 2,386,054 G→A R65R (AGG→AGA yfbL → putative peptidase YfbL
RA 2,429,677 T→C I188V (ATT→GTT)  purF ← amidophosphoribosyltransferase
RA 2,431,651 T→C T12A (ACG→GCG)  dedD ← cell division protein DedD
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,499,137 T→C V156A (GTA→GCA)  ypdA → sensory histidine kinase YpdA
RA 2,501,698 T→C V190A (GTT→GCT)  ypdC → putative AraC‑type DNA‑binding transcriptional regulator YpdC
RA 2,502,375 G→A A704V (GCG→GTG)  fryA ← putative PTS multiphosphoryl transfer protein FryA
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,589,906 T→C V771A (GTG→GCG)  acrD → multidrug efflux pump RND permease AcrD
RA 2,596,881 A→G intergenic (‑144/+24) ypfJ ← / ← purC uncharacterized protein YpfJ/phosphoribosylaminoimidazole‑succinocarboxamide synthase
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,603,492 T→C F559L (TTT→CTT)  hyfB → hydrogenase 4 component B
RA 2,638,026 (C)6→7 coding (627/1179 nt) bamB ← outer membrane protein assembly factor BamB
RA 2,695,419 T→C D42G (GAC→GGC)  purL ← phosphoribosylformylglycinamide synthetase
RA 2,725,940 T→C intergenic (‑194/+129) kgtP ← / ← rrfG alpha‑ketoglutarate:H(+) symporter/5S ribosomal RNA
RA 2,731,168 T→C intergenic (‑11/+432) rrsG ← / ← clpB 16S ribosomal RNA/ClpB chaperone
RA 2,732,082 G→A R698C (CGT→TGT)  clpB ← ClpB chaperone
RA 2,734,543 (C)8→10 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,736,212 T→C S23P (TCA→CCA)  bamD → outer membrane protein assembly factor BamD
RA 2,767,482 T→C intergenic (+127/‑228) rnlB → / → yfjP CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP
RA 2,797,288 A→G R26R (CGA→CGG ygaV → putative DNA‑binding transcriptional regulator YgaV
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,819,194 G→A intergenic (‑48/+187) csrA ← / ← alaS carbon storage regulator/alanine‑‑tRNA ligase/DNA‑binding transcriptional repressor
RA 2,827,043 G→A A218T (GCG→ACG)  srlE → sorbitol‑specific PTS enzyme IIBC1 component
RA 2,834,011 G→A A34T (GCC→ACC)  norW → NADH:flavorubredoxin reductase
RA 2,845,002 G→A N490N (AAC→AAT hycE ← formate hydrogenlyase subunit HycE
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,859,970 T→C F71L (TTT→CTT)  pphB → phosphoprotein phosphatase 2
RA 2,886,819 T→C D134G (GAT→GGT)  cas3 ← CRISPR‑associated endonuclease/helicase Cas3
RA 2,906,010 G→A W100* (TGG→TGA ygcG → protein YgcG
RA 2,942,448 T→C Q40Q (CAA→CAG gcvA ← DNA‑binding transcriptional dual regulator GcvA
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,976,076 A→G intergenic (‑62/+26) aas ← / ← omrA acyltransferase/small regulatory RNA OmrA
RA 2,983,415 A→G V237A (GTG→GCG)  kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 3,000,395 C→T D17D (GAC→GAT xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,001,304 (G)7→8 coding (960/2259 nt) xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,002,278 T→C V645A (GTA→GCA)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,052,554 T→C D322G (GAC→GGC)  ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase
RA 3,054,245 G→A A199V (GCG→GTG)  pepP ← proline aminopeptidase P II
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,108,233 A→G F308S (TTT→TCT)  speC ← ornithine decarboxylase, biosynthetic
RA 3,121,258 A→G Y8H (TAT→CAT)  glcA ← glycolate/lactate:H(+) symporter GlcA
RA 3,146,661 T→C K26R (AAA→AGA)  yghW ← DUF2623 domain‑containing protein YghW
RA 3,244,143 T→C Y200C (TAC→TGC)  uxaC ← D‑glucoronate/D‑galacturonate isomerase
RA 3,245,135 (T)8→7 intergenic (‑394/‑89) uxaC ← / → exuT D‑glucoronate/D‑galacturonate isomerase/hexuronate transporter
RA 3,272,907 C→T A217A (GCG→GCA garL ← alpha‑dehydro‑beta‑deoxy‑D‑glucarate aldolase
RA 3,284,724 G→A A14T (GCG→ACG)  agaC → galactosamine‑specific PTS enzyme IIC component
RA 3,305,770 G→A G16G (GGC→GGT mtr ← tryptophan:H(+) symporter Mtr
RA 3,309,066 T→C A701A (GCA→GCG pnp ← polynucleotide phosphorylase
RA 3,326,822 T→C L38L (CTA→CTG ftsH ← ATP‑dependent zinc metalloprotease FtsH
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,362,127 A→G T6A (ACA→GCA)  yhcA → putative fimbrial chaperone YhcA
RA 3,375,276 T→C G136G (GGT→GGC dcuD → putative transporter DcuD
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,399,078 A→G L301L (TTG→CTG)  mreC ← cell shape determining protein MreC
RA 3,407,849 G→A M81I (ATG→ATA panF → pantothenate:Na(+) symporter
RA 3,437,256 C→T L247L (CTG→TTG)  trkA → NAD‑binding component of Trk potassium transporters
RA 3,461,863 C→T I132I (ATC→ATT gspH → Type II secretion system protein GspH
RA 3,491,988 C→T G104S (GGC→AGC)  ppiA ← peptidyl‑prolyl cis‑trans isomerase A
RA 3,515,696 T→C Y73C (TAC→TGC)  dam ← DNA adenine methyltransferase
RA 3,542,962 T→C *79Q (TAA→CAA)  feoC → ferrous iron transport protein FeoC
RA 3,545,166 T→C R95R (CGT→CGC yhgH → DNA utilization protein YhgH
RA 3,545,888 T→C L89L (TTA→CTA)  nfuA → iron‑sulfur cluster carrier protein NfuA
RA 3,550,178 T→C T766A (ACT→GCT)  malP ← maltodextrin phosphorylase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,581,070 (A)7→8 intergenic (‑54/‑68) ryhB ← / → yhhY small regulatory RNA RyhB/N‑acetyltransferase YhhY
RA 3,588,812 C→T E123E (GAG→GAA ugpC ← sn‑glycerol 3‑phosphate ABC transporter ATP binding subunit
RA 3,697,872 T→C V472A (GTG→GCG)  bcsE → c‑di‑GMP‑binding protein BcsE
RA 3,800,268 (T)8→9 coding (698/699 nt) waaY ← lipopolysaccharide core heptose (II) kinase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,835,396 T→C K178K (AAA→AAG yicJ ← putative xyloside transporter YicJ
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,878,249 T→C Q624R (CAG→CGG)  gyrB ← DNA gyrase subunit B
RA 3,894,503 T→C V208A (GTC→GCC)  yieE → putative phosphopantetheinyl transferase
RA 3,924,004 T→C I544V (ATT→GTT)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 3,965,557 (T)8→7 coding (74/1266 nt) rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,969,103 A→G T25A (ACC→GCC)  wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,970,032 (G)6→7 coding (1002/1047 nt) wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,997,767 T→C G195G (GGT→GGC yigB → 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
RA 4,043,008 A→G L198L (CTA→CTG srkA → stress response kinase A
RA 4,085,064 C→T A253A (GCG→GCA fdoG ← formate dehydrogenase O subunit alpha
RA 4,156,864 C→T G5G (GGC→GGT argH → argininosuccinate lyase
RA 4,163,215 A→G V19A (GTG→GCG)  trmA ← tRNA m(5)U54 methyltransferase
RA 4,173,834 A→G E251E (GAA→GAG birA → DNA‑binding transcriptional repressor/biotin‑[acetyl‑CoA‑carboxylase] ligase BirA
RA 4,183,531 A→G T763A (ACC→GCC)  rpoB → RNA polymerase subunit beta
RA 4,200,176 (G)6→7 intergenic (+82/‑105) yjaG → / → hupA conserved protein YjaG/DNA‑binding protein HU‑alpha
RA 4,219,224 T→C V210A (GTA→GCA)  aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,224,648 A→G N274S (AAC→AGC)  metH → cobalamin‑dependent methionine synthase
RA 4,278,421 C→T intergenic (‑355/‑58) ryjA ← / → ghxP small RNA RyjA/guanine/hypoxanthine transporter GhxP
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,317,069 C→T G50G (GGG→GGA phnM ← RPnTP hydrolase
RA 4,324,474 A→G V231A (GTC→GCC)  phnC ← phosphonate ABC transporter ATP binding subunit
RA 4,329,694 T→C L112P (CTC→CCC)  yjcZ → uncharacterized protein YjcZ
RA 4,330,680 A→G D60G (GAC→GGC)  proP → osmolyte:H(+) symporter ProP
RA 4,348,226 A→G D173D (GAT→GAC dcuB ← anaerobic C4‑dicarboxylate transporter DcuB
RA 4,364,164 (C)5→4 coding (879/1698 nt) dsbD ← protein disulfide oxidoreductase ‑ DsbDreduced
RA 4,395,322 C→T A419A (GCC→GCT nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase
RA 4,422,036 C→T A110V (GCC→GTC)  ulaC → L‑ascorbate specific PTS enzyme IIA component
RA 4,440,503 A→G V238A (GTG→GCG)  ytfL ← putative inner membrane protein
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,448,009 G→A G39D (GGC→GAC) 
G79G (GGC→GGT
ytfP →
yzfA ←
gamma‑glutamylamine cyclotransferase family protein YtfP
putative YjfA
RA 4,487,255 C→T N346N (AAC→AAT lptF → lipopolysaccharide transport system protein LptF
RA 4,501,271 T→C P4P (CCT→CCC yjgZ → KpLE2 phage‑like element; uncharacterized protein YjgZ
RA 4,515,070 G→A D536D (GAC→GAT fecA ← ferric citrate outer membrane transporter
RA 4,537,245 G→A P117L (CCG→CTG)  nanS ← N‑acetyl‑9‑O‑acetylneuraminate esterase
RA 4,548,979 G→A D58N (GAT→AAT)  fimH → type 1 fimbriae D‑mannose specific adhesin
RA 4,585,505 C→T L419L (CTG→CTA hsdR ← type I restriction enzyme EcoKI endonuclease component
RA 4,592,406 Δ1 bp coding (750/1656 nt) tsr → methyl‑accepting chemotaxis protein Tsr
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,330 (G)8→10 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,608,076 A→G T92A (ACT→GCT)  holD → DNA polymerase III subunit psi
RA 4,628,092 G→A A260T (GCG→ACG)  nadR → DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR
RA 4,630,159 C→T A122T (GCC→ACC)  ettA ← energy‑dependent translational throttle protein EttA
RA 4,638,175 A→G intergenic (+55/‑3) creC → / → creD sensory histidine kinase CreC/putative inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 223870–224771 225464–224800 30–1595 12 [10] [10] 11 [rrsH]–ileV [rrsH],ileV
* * ÷ NC_000913 225664–225720 225898–225738 19–235 11 [10] [10] 14 [rrlH] [rrlH]
* * ÷ NC_000913 226146–226968 228561–226989 22–2416 11 [9] [9] 13 rrlH 23S ribosomal RNA
* * ÷ NC_000913 1633759–1634066 1634909–1634644 579–1151 13 [10] [10] 14 [pinQ]–[ydfN] [pinQ],tfaQ,[ydfN]
* * ÷ NC_000913 3423770–3424233 3424518–3424242 10–749 12 [9] [10] 11 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =12 (0.200)73 (1.370) 51/244 0.0 88.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 9 (0.170)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780512 (0.200)35 (0.660) 25/244 0.8 78.1% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 9 (0.170)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =137 (2.270)117 (1.950) 66/274 0.0 63.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)77 (1.280) 49/276 0.1 93.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.080)coding (3907/4134 nt) rhsA rhs element protein RhsA