breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 46,840 C→T A345V (GCC→GTC)  yaaU → putative transporter YaaU
RA 63,556 T→C Y742C (TAC→TGC)  polB ← DNA polymerase II
RA 78,721 (G)5→7 coding (1101/1179 nt) setA → sugar exporter SetA
RA 93,241 A→G T26A (ACA→GCA)  murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 103,198 T→C V15A (GTT→GCT)  ftsQ → cell division protein FtsQ
RA 107,553 (T)9→8 intergenic (+79/‑152) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/SecA translation regulator
RA 136,457 (T)8→7 coding (8/867 nt) speE ← spermidine synthase
RA 152,110 A→G V41A (GTA→GCA)  yadL ← putative fimbrial protein YadL
RA 178,095 (G)5→6 coding (368/801 nt) btuF ← vitamin B12 ABC transporter periplasmic binding protein
RA 181,996 T→C G371G (GGT→GGC degP → periplasmic serine endoprotease DegP
RA 216,269 C→A A245A (GCG→GCT yaeF ← peptidase C92 family protein
RA 235,772 A→G G77G (GGT→GGC rnhA ← ribonuclease HI
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 281,050 (G)5→6 coding (934/1155 nt) yagA ← CP4‑6 prophage; integrase core domain‑containing protein YagA
RA 317,251 T→C intergenic (‑115/+475) ykgA ← / ← rclC putative DNA‑binding transcriptional regulator YkgA/reactive chlorine species resistance protein C
RA 333,796 A→G V213A (GTG→GCG)  yahB ← putative LysR‑type DNA‑binding transcriptional regulator YahB
RA 356,058 A→G L379L (TTA→TTG codB → cytosine transporter
RA 359,829 A→G E114G (GAG→GGG)  cynS → cyanase
RA 380,022 (G)10→9 intergenic (‑141/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 434,513 A→G I353M (ATA→ATG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase
RA 477,430 T→C E396E (GAA→GAG pdeB ← c‑di‑GMP phosphodiesterase PdeB
RA 482,784 A→G R540R (CGT→CGC acrB ← multidrug efflux pump RND permease AcrB
RA 493,451 A→G T454A (ACC→GCC)  dnaX → DNA polymerase III subunit tau
RA 505,107 G→A G65D (GGT→GAT)  ushA → 5'‑nucleotidase/UDP‑sugar hydrolase
RA 510,729 G→A S217S (AGC→AGT copA ← Cu(+) exporting P‑type ATPase
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 548,964 Δ1 bp coding (608/1260 nt) ylbE → DUF1116 domain‑containing protein YlbE
RA 555,650 A→G D347G (GAC→GGC)  cysS → cysteine‑‑tRNA ligase
RA 591,428 C→A G584V (GGC→GTC)  nfrB ← bacteriophage N4 receptor, inner membrane subunit
RA 600,439 A→G T576A (ACG→GCG)  cusA → copper/silver export system RND permease
RA 605,132 A→G V98A (GTT→GCT)  nfsB ← NAD(P)H nitroreductase NfsB
RA 655,867 A→G G34G (GGT→GGC dcuC ← anaerobic C4‑dicarboxylate transporter DcuC
RA 735,986 (A)6→7 coding (337/570 nt) ybfC → uncharacterized protein YbfC
RA 737,376 A→G pseudogene (477/1062 nt) ybfL → putative transposase YbfL
RA 791,005 G→A H9Y (CAT→TAT)  galT ← galactose‑1‑phosphate uridylyltransferase
RA 805,840 G→A intergenic (+76/+158) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 805,853 Δ1 bp intergenic (+89/+145) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 805,855 T→C intergenic (+91/+143) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 805,863 A→G intergenic (+99/+135) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 805,872 A→G intergenic (+108/+126) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 805,876 T→A intergenic (+112/+122) ybhJ → / ← ybhC putative hydratase YbhJ/outer membrane lipoprotein YhbC
RA 817,144 A→G D34G (GAT→GGT)  moaA → GTP 3',8'‑cyclase
RA 819,624 C→T A110A (GCC→GCT moaE → molybdopterin synthase catalytic subunit
RA 845,350 (T)8→9 coding (131/2226 nt) ybiO ← moderate conductance mechanosensitive channel
RA 854,750 A→G I368M (ATA→ATG ybiR → putative transporter YbiR
RA 859,698 T→C S111G (AGT→GGT)  ybiV ← sugar phosphatase
RA 886,543 A→G V266A (GTT→GCT)  ybjJ ← inner membrane protein YbjJ
RA 888,832 A→G F330L (TTC→CTC)  ybjL ← putative transport protein YbjL
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 913,631 C→T V62I (GTT→ATT)  hcp ← protein S‑nitrosylase
RA 924,084 T→C L274P (CTG→CCG)  clpA → ATP‑dependent Clp protease ATP‑binding subunit ClpA
JC 925,899 (G)7→10 noncoding (73/88 nt) serW ← tRNA‑Ser
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 989,044 (G)6→7 intergenic (‑59/+110) asnS ← / ← pncB asparagine‑‑tRNA ligase/nicotinate phosphoribosyltransferase
RA 993,073 C→T R818C (CGT→TGT)  pepN → aminopeptidase N
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,011,593 (A)7→8 coding (1630/1908 nt) uup → ATP‑binding protein with possible role in replication
RA 1,013,756 (A)6→7 coding (498/1641 nt) pqiB → intermembrane transport protein PqiB
RA 1,015,783 A→G G23G (GGA→GGG rmf → ribosome modulation factor
RA 1,019,827 T→C Y76C (TAC→TGC)  ompA ← outer membrane porin A
RA 1,048,289 A→G L135L (TTG→CTG)  gfcC ← capsule biosynthesis GfcC family protein
RA 1,093,865 (G)5→6 coding (990/1359 nt) dgcT → putative diguanylate cyclase DgcT
RA 1,103,670 C→T intergenic (‑474/‑281) csgD ← / → csgB DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit
RA 1,131,261 A→G T82A (ACC→GCC)  flgB → flagellar basal‑body rod protein FlgB
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,154,463 A→G M118V (ATG→GTG)  mltG → endolytic murein transglycosylase
RA 1,159,369 T→C Q728R (CAA→CGA)  fhuE ← ferric coprogen/ferric rhodotorulic acid outer membrane transporter
RA 1,167,348 A→G S422G (AGT→GGT)  ndh → NADH:quinone oxidoreductase II
RA 1,178,196 A→G M293V (ATG→GTG)  lolE → lipoprotein release complex ‑ inner membrane subunit
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,231,808 A→G D208G (GAT→GGT)  umuC → DNA polymerase V catalytic protein
RA 1,240,112 G→A intergenic (+163/‑148) dadX → / → ymgM alanine racemase 2/protein YmgM
RA 1,240,528 C→T G515D (GGC→GAC)  cvrA ← putative K(+):H(+) antiporter
RA 1,286,999 C→T intergenic (+15/+67) ychS → / ← rttR putative uncharacterized protein YchS/small RNA RttR
RA 1,289,658 T→C S138S (AGT→AGC rssA → putative patatin‑like phospholipase RssA
RA 1,302,129 T→C R316R (CGT→CGC oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,314,053 A→G T12A (ACA→GCA)  ompW → outer membrane protein W
RA 1,314,648 A→G Y210C (TAT→TGT)  ompW → outer membrane protein W
RA 1,324,113 (T)7→6 coding (16/621 nt) yciO → putative RNA‑binding protein YciO
RA 1,352,655 A→G L325P (CTG→CCG)  sapD ← putrescine ABC exporter ATP binding protein SapD
RA 1,352,747 Δ1 bp coding (882/993 nt) sapD ← putrescine ABC exporter ATP binding protein SapD
RA 1,373,237 (T)6→5 coding (16/882 nt) ycjO → putative ABC transporter membrane subunit YcjO
RA 1,392,761 (A)8→9 coding (771/900 nt) pgrR → DNA‑binding transcriptional repressor PgrR
RA 1,419,721 (G)7→6 intergenic (‑265/+44) ydaG ← / ← racR Rac prophage; uncharacterized protein YdaG/Rac prophage; DNA‑binding transcriptional repressor RacR
RA 1,426,089 T→C intergenic (+109/‑365) ydaY → / → ynaA Rac prophage; putative uncharacterized protein YdaY/Rac prophage; putative prophage tail length tape measure domain‑containing protein YnaA
RA 1,437,231 (T)9→8 intergenic (+2/+29) micC → / ← ydbK small regulatory RNA MicC/putative pyruvate‑flavodoxin oxidoreductase
RA 1,437,788 A→G G999G (GGT→GGC ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,495,485 A→G E66E (GAA→GAG ydcJ → DUF1338 domain‑containing protein YdcJ
RA 1,499,118 T→C L61L (TTG→CTG)  rimL → ribosomal‑protein‑L12‑serine acetyltransferase
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,544,042 (T)5→4 coding (19/1389 nt) narU ← nitrate/nitrite transporter NarU
RA 1,550,878 A→G I140V (ATT→GTT)  fdnH → formate dehydrogenase N subunit beta
RA 1,555,655 (T)7→8 coding (15/1698 nt) maeA ← malate dehydrogenase, NAD‑requiring
RA 1,577,059 A→G T187T (ACT→ACC yddB ← putative TonB‑dependent receptor
RA 1,587,142 T→C D198G (GAC→GGC)  ydeQ ← putative fimbrial adhesin protein YdeQ
RA 1,597,313 (C)7→6 pseudogene (774/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,603,815 T→C V266A (GTA→GCA)  lsrC → Autoinducer‑2 ABC transporter membrane subunit LsrC
RA 1,607,262 (A)6→7 coding (264/291 nt) lsrG → (4S)‑4‑hydroxy‑5‑phosphonooxypentane‑2,3‑dione isomerase
RA 1,655,044 T→C D33G (GAT→GGT)  rspA ← mandelate racemase/muconate lactonizing enzyme family protein RspA
RA 1,675,608 (C)5→6 coding (753/1389 nt) pntB ← pyridine nucleotide transhydrogenase subunit beta
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,695,724 G→A A116A (GCC→GCT uidA ← beta‑glucuronidase
RA 1,699,694 T→C F114L (TTC→CTC)  malX → PTS enzyme IIBC component MalX
RA 1,704,167 G→A P48S (CCA→TCA)  ydgJ ← putative oxidoreductase YdgJ
RA 1,709,945 A→G A268A (GCA→GCG rsxD → SoxR [2Fe‑2S] reducing system protein RsxD
RA 1,712,518 (C)6→7 intergenic (+360/‑251) nth → / → dtpA endonuclease III/dipeptide/tripeptide:H(+) symporter DtpA
RA 1,749,745 A→G C202R (TGT→CGT)  ydhU ← putative cytochrome YdhU
RA 1,753,415 Δ1 bp coding (416/2103 nt) ydhV ← putative oxidoreductase
RA 1,754,383 G→A A32V (GCC→GTC)  ydhY ← putative 4Fe‑4S ferredoxin‑type protein
RA 1,764,003 G→A R3C (CGT→TGT)  sufB ← Fe‑S cluster scaffold complex subunit SufB
RA 1,806,659 T→C V97A (GTG→GCG)  pfkB → 6‑phosphofructokinase II
RA 1,812,736 A→G L133L (TTG→CTG)  ydjO ← protein YdjO
RA 1,818,550 (C)7→8 intergenic (‑50/+55) chbF ← / ← chbR monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR
RA 1,853,893 A→G F29F (TTT→TTC ydjE ← putative transporter YdjE
RA 1,860,891 C→T V148I (GTT→ATT)  ydjL ← putative zinc‑binding dehydrogenase YdjL
RA 1,864,670 C→T T274M (ACG→ATG)  yeaD → putative aldose 1‑epimerase YeaD
RA 1,865,169 (C)6→7 coding (468/855 nt) yeaE ← methylglyoxal reductase YeaE
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,877,866 A→G Q51R (CAG→CGG)  dgcP → diguanylate cyclase DgcP
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,882,756 T→C V282A (GTC→GCC)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,920,963 T→C C247C (TGT→TGC rsmF → 16S rRNA m(5)C1407 methyltransferase
RA 1,934,179 A→G H142H (CAT→CAC edd ← phosphogluconate dehydratase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,012,563 (G)6→7 pseudogene (62/279 nt) intG → putative defective phage integrase
RA 2,046,898 T→C V654A (GTG→GCG)  yeeJ → inverse autotransporter adhesin
RA 2,051,982 T→C F2349L (TTT→CTT)  yeeJ → inverse autotransporter adhesin
RA 2,071,189 T→C pseudogene (404/426 nt) yeeP → CP4‑44 prophage; putative GTP‑binding protein
RA 2,072,464 A→G K309R (AAG→AGG)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,073,366 A→G T610A (ACC→GCC)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,081,698 T→C T284A (ACG→GCG)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase DacD
RA 2,091,712 Δ1 bp coding (616/1305 nt) hisD → histidinal/histidinol dehydrogenase
RA 2,125,570 T→C K59K (AAA→AAG wcaI ← putative colanic biosynthesis glycosyl transferase
RA 2,132,104 A→G V151A (GTG→GCG)  wcaB ← putative colanic acid biosynthesis acyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,185,726 G→A A24V (GCG→GTG)  rcnR ← DNA‑binding transcriptional repressor RcnR
RA 2,193,357 A→G V271A (GTG→GCG)  mrp ← P‑loop NTPase family protein
RA 2,232,893 T→C F6L (TTC→CTC)  sanA → DUF218 domain‑containing protein SanA
RA 2,240,754 (C)5→4 coding (915/1041 nt) galS ← DNA‑binding transcriptional dual regulator GalS
RA 2,247,128 G→A L469L (CTG→TTG)  lysP ← lysine:H(+) symporter
RA 2,270,127 T→C L50P (CTG→CCG)  mepS → peptidoglycan DD‑endopeptidase/peptidoglycan LD‑carboxypeptidase
RA 2,295,852 G→A R157C (CGT→TGT)  ccmC ← heme trafficking system membrane protein CcmC
RA 2,336,634 (T)7→6 coding (11/1689 nt) yfaA ← DUF2138 domain‑containing protein YfaA
RA 2,381,001 A→G R9R (CGT→CGC elaB ← tail anchored inner membrane protein
RA 2,430,323 (A)7→8 coding (441/489 nt) cvpA ← colicin V production protein
RA 2,442,697 T→C Y312H (TAT→CAT)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,455,256 T→C T131A (ACC→GCC)  yfcV ← putative fimbrial protein YfcV
RA 2,457,177 T→C G661G (GGA→GGG fadJ ← 3‑hydroxyacyl‑CoA dehydrogenase FadJ
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,484,057 T→C S101S (AGT→AGC evgA → DNA‑binding transcriptional activator EvgA
RA 2,496,075 G→A G144E (GGA→GAA)  lpxP → palmitoleoyl acyltransferase
RA 2,497,153 G→A D381D (GAC→GAT alaC ← glutamate‑‑pyruvate aminotransferase AlaC
RA 2,500,410 T→C F10L (TTC→CTC)  ypdB → DNA‑binding transcriptional activator YpdB
RA 2,507,334 T→C L187L (TTA→TTG fryC ← putative PTS enzyme IIC component FryC
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,543,052 A→G R159R (CGT→CGC cysP ← thiosulfate/sulfate ABC transporter periplasmic binding protein CysP
RA 2,579,515 C→T H284Y (CAC→TAC)  talA → transaldolase A
RA 2,598,045 C→T G274D (GGC→GAC)  bamC ← outer membrane protein assembly factor BamC
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,604,172 T→C I109T (ATC→ACC)  hyfC → hydrogenase 4 component C
RA 2,620,850 T→C H8R (CAC→CGC)  upp ← uracil phosphoribosyltransferase
RA 2,632,973 A→G G366G (GGT→GGC guaB ← inosine 5'‑monophosphate dehydrogenase
RA 2,663,078 A→G R82R (CGT→CGC trmJ ← tRNA Cm32/Um32 methyltransferase
RA 2,690,350 C→A R250L (CGC→CTC)  glrK ← sensory histidine kinase GlrK
RA 2,734,543 (C)8→9 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,737,713 (G)6→7 intergenic (+67/‑32) pheL → / → pheA phe operon leader peptide/fused chorismate mutase/prephenate dehydratase
RA 2,738,236 T→C G164G (GGT→GGC pheA → fused chorismate mutase/prephenate dehydratase
RA 2,770,922 G→A G81D (GGT→GAT)  yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,770,938 T→C Y86Y (TAT→TAC yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,770,941 G→C L87L (CTG→CTC yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,770,944 A→G V88V (GTA→GTG yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,770,951 G→C E91Q (GAG→CAG)  yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,770,956 G→A E92E (GAG→GAA yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,770,971 G→A P97P (CCG→CCA yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,771,040 G→C T120T (ACG→ACC yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,771,055 C→T F125F (TTC→TTT yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,771,058 A→G E126E (GAA→GAG yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,771,061 2 bp→CG coding (381‑382/444 nt) yfjS → CP4‑57 prophage; inner membrane lipoprotein YfjS
RA 2,776,420 T→C V12A (GTA→GCA)  yfjY → CP4‑57 prophage; RadC‑like JAB domain‑containing protein YfjY
RA 2,807,382 T→C V84A (GTG→GCG)  proX → glycine betaine ABC transporter periplasmic binding protein ProX
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,852,654 C→T C173C (TGC→TGT hypD → Fe‑(CN)2CO cofactor assembly scaffold protein HypD
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,872,552 T→C D90G (GAC→GGC)  ftsB ← cell division protein FtsB
RA 2,925,689 G→A T114T (ACG→ACA queF → 7‑cyano‑7‑deazaguanine reductase
RA 2,943,707 T→C V124A (GTC→GCC)  csdA → cysteine sulfinate desulfinase
RA 2,947,343 (A)8→7 intergenic (‑165/‑44) mltA ← / → metZ membrane‑bound lytic murein transglycosylase A/tRNA‑Met
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,965,754 A→G V95A (GTC→GCC)  lgt ← phosphatidylglycerol‑‑prolipoprotein diacylglyceryl transferase
RA 2,966,716 T→C E573E (GAA→GAG ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 3,001,304 (G)7→8 coding (960/2259 nt) xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
JC 3,003,479 (G)6→9 coding (866/879 nt) xdhB → putative xanthine dehydrogenase FAD‑binding subunit XdhB
RA 3,009,850 A→T I363F (ATC→TTC)  ygeY → putative peptidase YgeY
RA 3,051,344 T→C D325G (GAC→GGC)  ubiI ← 2‑octaprenylphenol hydroxylase
RA 3,060,561 A→G H270R (CAC→CGC)  argP → DNA‑binding transcriptional dual regulator ArgP
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,069,712 C→T V32V (GTG→GTA mscS ← small conductance mechanosensitive channel MscS
RA 3,082,192 T→C Y94H (TAT→CAT)  loiP → metalloprotease LoiP
RA 3,108,382 G→A R258R (CGC→CGT speC ← ornithine decarboxylase, biosynthetic
RA 3,123,593 A→G N71N (AAT→AAC glcB ← malate synthase G
RA 3,158,920 A→G intergenic (+36/+7) yqhH → / ← ygiQ lipoprotein YqhH/radical SAM superfamily protein YgiQ
RA 3,160,127 A→G P340P (CCT→CCC ygiQ ← radical SAM superfamily protein YgiQ
RA 3,191,379 A→G T250A (ACG→GCG)  yqiI → putative fimbrial protein YqiI
RA 3,198,044 A→G V533A (GTG→GCG)  glnE ← fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
RA 3,229,583 T→C G232G (GGT→GGC ygjK → glycoside hydrolase
RA 3,263,316 T→C A115A (GCA→GCG tdcD ← propionate kinase
RA 3,270,488 (C)7→8 noncoding (105/377 nt) rnpB ← RNase P catalytic RNA component
RA 3,290,126 A→G D438G (GAC→GGC)  yraJ → putative fimbrial usher protein YraJ
RA 3,300,074 Δ1 bp coding (109/303 nt) yhbQ → DNA damage response nuclease YhbQ
JC 3,337,101 (C)6→9 coding (156/294 nt) mlaB ← intermembrane phospholipid transport system protein MlaB
RA 3,347,536 T→C V141A (GTT→GCT)  rapZ → RNase adaptor protein
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,362,424 T→C F105L (TTC→CTC)  yhcA → putative fimbrial chaperone YhcA
RA 3,375,895 (G)6→5 coding (1027/1368 nt) dcuD → putative transporter DcuD
RA 3,386,282 T→C E636G (GAA→GGA)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,398,446 G→A L144F (CTC→TTC)  mreD ← cell shape determining protein MreD
RA 3,407,296 Δ1 bp intergenic (+30/‑79) accC → / → yhdT biotin carboxylase/DUF997 domain‑containing protein YhdT
RA 3,420,535 T→C I134I (ATT→ATC yhdX → putative ABC transporter membrane subunit YhdX
RA 3,457,425 T→C V350A (GTT→GCT)  gspD → Type II secretion system protein GspD
RA 3,462,844 (G)6→7 coding (501/588 nt) gspJ → Type II secretion system protein GspJ
RA 3,463,197 A→G T92A (ACA→GCA)  gspK → Type II secretion system protein GspK
JC JC 3,470,056 IS1 (–) +8 bp intergenic (‑203/+82) chiA ← / ← tufA endochitinase/translation elongation factor Tu 1
RA 3,518,153 C→T G112S (GGC→AGC)  aroB ← 3‑dehydroquinate synthase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,564,565 T→C T673A (ACG→GCG)  glgP ← glycogen phosphorylase
RA 3,573,754 C→T intergenic (‑252/+21) glgB ← / ← asd 1,4‑alpha‑glucan branching enzyme/aspartate‑semialdehyde dehydrogenase
RA 3,576,313 A→G V250A (GTG→GCG)  gntU ← low‑affinity gluconate transporter
MC JC 3,581,940 Δ1,488 bp IS1‑mediated [yhhZ][yrhA] [yhhZ], [yrhA]
RA 3,606,118 C→T T123M (ACG→ATG)  yhhN → conserved inner membrane enzyme YhhN
RA 3,629,501 G→A A13V (GCG→GTG)  rbbA ← ribosome‑associated ATPase
RA 3,664,399 A→G L74L (TTA→CTA)  gadW ← DNA‑binding transcriptional dual regulator GadW
RA 3,740,385 T→C G227G (GGT→GGC avtA → valine‑‑pyruvate aminotransferase
RA 3,750,873 A→G intergenic (+92/+255) sgbE → / ← yiaT L‑ribulose‑5‑phosphate 4‑epimerase SgbE/outer membrane protein YiaT
RA 3,753,644 G→A S152S (AGC→AGT yiaV ← putative membrane fusion protein
RA 3,756,083 A→G G143G (GGT→GGC aldB ← aldehyde dehydrogenase B
RA 3,783,799 A→G A110A (GCT→GCC secB ← SecB chaperone
RA 3,807,692 A→G L136P (CTT→CCT)  waaQ ← lipopolysaccharide core heptosyltransferase 3
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,854,055 T→C V45A (GTG→GCG)  emrD → multidrug efflux pump EmrD
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,863,379 T→C L75L (TTA→TTG glvC ← putative PTS enzyme II component GlvC
RA 3,881,409 T→C I305V (ATC→GTC)  dnaN ← beta sliding clamp
RA 3,894,592 G→A A238T (GCC→ACC)  yieE → putative phosphopantetheinyl transferase
RA 3,915,903 T→C I24V (ATC→GTC)  atpC ← ATP synthase F1 complex subunit epsilon
RA 3,930,842 A→G G84G (GGT→GGC ravA ← regulatory ATPase RavA
RA 3,935,976 T→C L230P (CTG→CCG)  rbsC → ribose ABC transporter membrane subunit
RA 3,937,409 C→T A39V (GCA→GTA)  rbsK → ribokinase
RA 3,944,122 T→C noncoding (419/2904 nt) rrlC → 23S ribosomal RNA
RA 3,945,556 A→G noncoding (1853/2904 nt) rrlC → 23S ribosomal RNA
RA 3,955,349 C→T L7L (CTG→TTG)  ilvA → threonine deaminase
RA 3,972,840 A→G T107A (ACA→GCA)  rffG → dTDP‑glucose 4,6‑dehydratase 2
RA 3,982,684 (C)8→9 noncoding (79/87 nt) leuT → tRNA‑Leu
RA 3,994,961 G→T G67V (GGC→GTC)  dapF → diaminopimelate epimerase
RA 4,018,153 A→G Y274C (TAC→TGC)  rmuC → putative recombination limiting protein RmuC
RA 4,023,436 G→A R234H (CGC→CAC)  tatC → twin arginine protein translocation system ‑ TatC protein
RA 4,043,282 T→C F290L (TTC→CTC)  srkA → stress response kinase A
RA 4,068,440 G→A G279G (GGC→GGT yihQ ← sulfoquinovosidase
RA 4,069,512 T→C H297R (CAC→CGC)  yihR ← putative aldose 1‑epimerase YihR
RA 4,105,864 C→T V15V (GTC→GTT cpxP → periplasmic protein CpxP
RA 4,155,941 C→T A314V (GCG→GTG)  argC → N‑acetylglutamylphosphate reductase
RA 4,165,497 G→A V24M (GTG→ATG)  murI → glutamate racemase
RA 4,209,713 G→A intergenic (+25/‑61) rrsE → / → gltV 16S ribosomal RNA/tRNA‑Glu
RA 4,232,375 T→C T287A (ACG→GCG)  lysC ← aspartate kinase III
RA 4,236,508 A→G T83A (ACT→GCT)  yjbF → lipoprotein YjbF
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,351,784 T→C intergenic (‑122/‑59) dcuS ← / → yjdI sensory histidine kinase DcuS/PF06902 family protein YjdI
RA 4,358,040 T→C H193R (CAC→CGC)  cadA ← lysine decarboxylase 1
RA 4,401,719 A→G I350V (ATA→GTA)  hflX → ribosome rescue factor HflX
RA 4,419,193 Δ1 bp coding (433/1065 nt) ulaG ← L‑ascorbate‑6‑phosphate lactonase
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,448,792 T→C V34A (GTG→GCG)  chpB → endoribonuclease toxin ChpB
RA 4,449,073 (A)7→6 intergenic (+31/+49) chpB → / ← ppa endoribonuclease toxin ChpB/inorganic pyrophosphatase
RA 4,509,750 A→G pseudogene (272/315 nt) insO → KpLE2 phage‑like element; IS911B regulator fragment
RA 4,513,105 G→A A102V (GCG→GTG)  fecC ← ferric citrate ABC transporter membrane subunit FecC
RA 4,518,721 A→G G65G (GGA→GGG insA‑7 → KpLE2 phage‑like element; IS1 repressor protein InsA
RA 4,544,301 A→G intergenic (+34/‑3) fimI → / → fimC putative fimbrial protein FimI/type 1 fimbriae periplasmic chaperone
RA 4,547,436 A→G T781A (ACT→GCT)  fimD → type I fimbriae usher protein
RA 4,567,286 (A)5→4 intergenic (‑40/+1) yjiN ← / ← mdtM DUF445 domain‑containing protein YjiN/multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 4,569,491 A→G pseudogene (494/921 nt) rpnD → truncated recombination‑promoting nuclease‑like protein RpnD
RA 4,571,048 C→T G176D (GGC→GAC)  yjiR ← fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
RA 4,606,215 (G)8→10 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,607,596 C→T A35A (GCG→GCA rsmC ← 16S rRNA m(2)G1207 methyltransferase
RA 4,637,666 T→C V324A (GTT→GCT)  creC → sensory histidine kinase CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 734881–734938 734977–734943 6–97 7 [6] [5] 7 ybfO conserved protein, rhs‑like YbfO
* * ÷ NC_000913 1196292 1211419 15128 7 [5] [6] 8 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1633325–1633923 1634911–1634647 725–1587 7 [5] [6] 7 pinQ–[ydfN] pinQ,tfaQ,[ydfN]
* * ÷ NC_000913 2304661–2304783 2304922–2304802 20–262 7 [6] [6] 7 eco/mqo serine protease inhibitor ecotin/malate:quinone oxidoreductase
* * ÷ NC_000913 2770984–2770997 2771025 29–42 10 [5] [5] 8 yfjS CP4‑57 prophage; inner membrane lipoprotein YfjS
* * ÷ NC_000913 3423787–3424234 3424518–3424239 6–732 7 [6] [2] 7 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3619340–3622297 3622416–3622379 83–3077 7 [6] [6] 7 rhsB rhs element protein RhsB
* * ÷ NC_000913 4168394–4168477 4171635–4168572 96–3242 7 [6] [3] 7 [gltT]–rrlB [gltT],rrlB

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)53 (1.250) 38/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =79 (1.800)87 (2.000) 53/274 0.0 68.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)56 (1.280) 43/276 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =41 (0.940)12 (0.290) 9/258 2.1 20.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 57 (1.390)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit