breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 31,629 G→A 7.7% V271V (GTG→GTA carB → carbamoyl‑phosphate synthase large subunit
RA 67,858 G→A 13.9% I160I (ATC→ATT araA ← L‑arabinose isomerase
RA 70,434 C→T 91.5% N16N (AAC→AAT araC → DNA‑binding transcriptional dual regulator
RA 70,581 C→T 89.5% V65V (GTC→GTT araC → DNA‑binding transcriptional dual regulator
RA 70,740 T→G 93.7% G118G (GGT→GGG araC → DNA‑binding transcriptional dual regulator
RA 71,079 C→T 93.5% R231R (CGC→CGT araC → DNA‑binding transcriptional dual regulator
RA 71,082 T→C 93.8% I232I (ATT→ATC araC → DNA‑binding transcriptional dual regulator
RA 71,085 T→C 92.9% S233S (AGT→AGC araC → DNA‑binding transcriptional dual regulator
RA 71,109 T→C 91.0% T241T (ACT→ACC araC → DNA‑binding transcriptional dual regulator
RA 71,175 A→G 93.1% R263R (CGA→CGG araC → DNA‑binding transcriptional dual regulator
RA 71,214 T→C 91.1% F276F (TTT→TTC araC → DNA‑binding transcriptional dual regulator
RA 77,316 T→C 9.4% intergenic (‑17/‑305) sgrR ← / → setA DNA‑binding transcriptional regulator/broad specificity sugar efflux system
RA 120,479 G→A 10.5% A358V (GCT→GTT)  aroP ← aromatic amino acid transporter
RA 155,463 A→T 100% *247K (TAA→AAA)  ecpD ← predicted periplasmic pilin chaperone
RA 181,321 C→T 10.4% G146G (GGC→GGT degP → serine endoprotease (protease Do), membrane‑associated
RA 181,474 T→C 10.8% F197F (TTT→TTC degP → serine endoprotease (protease Do), membrane‑associated
RA 181,477 T→C 9.7% G198G (GGT→GGC degP → serine endoprotease (protease Do), membrane‑associated
RA 181,489 G→A 8.9% T202T (ACG→ACA degP → serine endoprotease (protease Do), membrane‑associated
RA 236,101 A→C 92.8% D12A (GAT→GCT)  dnaQ → DNA polymerase III epsilon subunit
RA 236,107 A→C 92.7% E14A (GAA→GCA)  dnaQ → DNA polymerase III epsilon subunit
RA 363,547 A→G 17.8% G661G (GGT→GGC lacZ ← beta‑D‑galactosidase
RA 363,556 C→T 13.9% A658A (GCG→GCA lacZ ← beta‑D‑galactosidase
RA 363,576 G→A 12.1% L652F (CTC→TTC)  lacZ ← beta‑D‑galactosidase
RA 383,415 G→A 12.9% C142C (TGC→TGT yaiS ← conserved hypothetical protein
RA 393,537 T→C 11.6% F433F (TTT→TTC yaiU → hypothetical protein
RA 427,941 T→C 11.6% T357T (ACT→ACC secD → SecYEG protein translocase auxillary subunit
RA 430,884 G→A 7.7% F118F (TTC→TTT tsx ← nucleoside channel, receptor of phage T6 and colicin K
RA 436,226 G→A 15.7% V138V (GTG→GTA pgpA → phosphatidylglycerophosphatase A
RA 436,250 C→T 12.2% I146I (ATC→ATT pgpA → phosphatidylglycerophosphatase A
RA 547,694 A→G 100% pseudogene (114/261 nt)
pseudogene (114/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
RA 552,978 C→T 14.7% A62A (GCG→GCA lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphatase
RA 552,981 C→T 15.1% A61A (GCG→GCA lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphatase
RA 552,984 C→G 15.1% A60A (GCG→GCC lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphatase
RA 552,992 T→G 15.2% K58Q (AAG→CAG)  lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphatase
RA 552,994 C→T 14.3% R57H (CGC→CAC)  lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphatase
RA 552,999 G→A 15.1% L55L (CTC→CTT lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphatase
RA 553,023 A→G 16.7% I47I (ATT→ATC lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphatase
RA 553,029 T→G 17.3% A45A (GCA→GCC lpxH ← UDP‑2,3‑diacylglucosamine pyrophosphatase
RA 556,858 A→T 100% L36Q (CTG→CAG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 575,770 G→A 100% pseudogene (339/1128 nt) nmpC ← DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
RA 598,515 C→T 20.6% P193P (CCC→CCT cusA → copper/silver efflux system, membrane component
RA 644,865 A→T 8.0% V313V (GTT→GTA citT ← citrate:succinate antiporter
MC JC 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]
RA 670,276 A→C 11.5% L18L (CTT→CTG cobC ← predicted alpha‑ribazole‑5'‑P phosphatase
RA 670,287 T→C 10.5% I15V (ATC→GTC)  cobC ← predicted alpha‑ribazole‑5'‑P phosphatase
RA 685,138 C→G 16.4% V180V (GTG→GTC gltL ← glutamate and aspartate transporter subunit
RA 685,153 T→C 19.6% V175V (GTA→GTG gltL ← glutamate and aspartate transporter subunit
RA 686,570 T→G 19.1% S174S (TCA→TCC gltJ ← glutamate and aspartate transporter subunit
RA 701,210 A→G 18.2% A269A (GCT→GCC nagC ← DNA‑binding transcriptional dual regulator
RA 726,688 C→T 14.8% A257A (GCG→GCA kdpB ← potassium translocating ATPase, subunit B
RA 740,339 T→G 7.3% P137P (CCT→CCG phr → deoxyribodipyrimidine photolyase, FAD‑binding
RA 740,342 C→G 7.0% G138G (GGC→GGG phr → deoxyribodipyrimidine photolyase, FAD‑binding
RA 740,354 C→T 7.4% T142T (ACC→ACT phr → deoxyribodipyrimidine photolyase, FAD‑binding
RA 775,916 C→T 12.5% I114I (ATC→ATT tolQ → membrane spanning protein in TolA‑TolQ‑TolR complex
RA 775,925 C→T 11.3% N117N (AAC→AAT tolQ → membrane spanning protein in TolA‑TolQ‑TolR complex
RA 775,958 G→T 10.3% P128P (CCG→CCT tolQ → membrane spanning protein in TolA‑TolQ‑TolR complex
RA 797,279 T→G 7.1% S102A (TCG→GCG)  modC → molybdate transporter subunit
RA 797,287 T→C 6.9% S104S (AGT→AGC modC → molybdate transporter subunit
RA 797,294 G→A 6.3% D107N (GAT→AAT)  modC → molybdate transporter subunit
RA 798,591 A→T 11.1% I88N (ATT→AAT)  ybhA ← predicted hydrolase
RA 811,900 T→A 11.4% A366A (GCT→GCA bioF → 8‑amino‑7‑oxononanoate synthase
RA 902,100 T→G 10.2% T191T (ACA→ACC artQ ← arginine transporter subunit
RA 902,106 C→T 9.2% L189L (CTG→CTA artQ ← arginine transporter subunit
RA 903,018 T→A 8.8% V131V (GTA→GTT artI ← arginine transporter subunit
RA 912,872 T→A 7.7% A455A (GCA→GCT hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 944,111 T→C 14.5% V92V (GTT→GTC dmsB → dimethyl sulfoxide reductase, anaerobic, subunit B
RA 944,114 G→T 14.2% V93V (GTG→GTT dmsB → dimethyl sulfoxide reductase, anaerobic, subunit B
RA 944,132 C→T 17.4% C99C (TGC→TGT dmsB → dimethyl sulfoxide reductase, anaerobic, subunit B
RA 1,017,036 C→T 6.6% A562A (GCG→GCA ycbZ ← predicted peptidase
RA 1,054,525 T→G 11.9% L692L (CTA→CTC torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,093,686 T→C 100% V130A (GTA→GCA)  ycdT → predicted diguanylate cyclase
RA 1,094,325 A→G 9.4% N343S (AAT→AGT)  ycdT → predicted diguanylate cyclase
RA 1,101,395 G→A 10.2% R177R (CGC→CGT csgG ← outer membrane lipoprotein
RA 1,174,888 C→A 13.8% P218P (CCG→CCT mfd ← transcription‑repair coupling factor
RA 1,180,003 T→C 12.7% D369D (GAT→GAC lolE → outer membrane‑specific lipoprotein transporter subunit
RA 1,180,007 C→T 12.0% L371L (CTG→TTG)  lolE → outer membrane‑specific lipoprotein transporter subunit
RA 1,237,886 A→G 11.6% F311F (TTT→TTC ycgB ← conserved hypothetical protein
RA 1,285,540 G→A 43.7% S120S (TCG→TCA narH → nitrate reductase 1, beta (Fe‑S) subunit
MC JC 1,301,041 Δ1,336 bp 100% insCinsD insC, insD
RA 1,302,548 A→G 13.1% intergenic (+190/‑348) insD → / → oppA IS2 element protein/oligopeptide transporter subunit
RA 1,302,550 A→T 12.8% intergenic (+192/‑346) insD → / → oppA IS2 element protein/oligopeptide transporter subunit
RA 1,303,631 C→T 100% Q246Y (CAG→TAT)  oppA → oligopeptide transporter subunit
RA 1,303,633 G→T 100% Q246Y (CAG→TAT oppA → oligopeptide transporter subunit
RA 1,313,544 A→G 42.8% intergenic (+22/+18) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD complex/predicted hydrolase
RA 1,313,545 C→T 45.8% intergenic (+23/+17) tonB → / ← yciA membrane spanning protein in TonB‑ExbB‑ExbD complex/predicted hydrolase
RA 1,355,750 A→G 10.6% A161A (GCT→GCC sapC ← predicted antimicrobial peptide transporter subunit
RA 1,355,756 G→A 8.9% S159S (TCC→TCT sapC ← predicted antimicrobial peptide transporter subunit
RA 1,442,292 G→A 8.7% G69G (GGC→GGT ydbK ← fused predicted Fe‑S subunit of pyruvate‑flavodoxin oxidoreductase
RA 1,661,831 A→T 10.9% R683S (AGA→AGT ynfE → oxidoreductase subunit
RA 1,661,832 A→G 10.9% T684A (ACC→GCC)  ynfE → oxidoreductase subunit
RA 1,662,156 C→G 20.3% L792V (CTG→GTG)  ynfE → oxidoreductase subunit
RA 1,724,458 A→T 7.9% A208A (GCA→GCT ydhK → conserved inner membrane protein
RA 1,726,822 G→A 15.9% T175T (ACC→ACT ydhF ← predicted oxidoreductase
RA 1,726,828 C→T 12.7% P173P (CCG→CCA ydhF ← predicted oxidoreductase
RA 1,726,834 A→G 11.7% R171R (CGT→CGC ydhF ← predicted oxidoreductase
RA 1,739,599 C→A 13.4% S14S (TCC→TCA purR → DNA‑binding transcriptional repressor, hypoxanthine‑binding
RA 1,748,488 A→G 11.1% P25P (CCA→CCG ydhR → hypothetical protein
RA 1,927,471 T→A 9.3% P106P (CCT→CCA yobB → conserved hypothetical protein
RA 1,927,490 C→T 9.4% H113Y (CAT→TAT)  yobB → conserved hypothetical protein
RA 1,951,613 A→G 7.7% R208R (CGT→CGC aspS ← aspartyl‑tRNA synthetase
RA 1,951,622 A→G 7.4% G205G (GGT→GGC aspS ← aspartyl‑tRNA synthetase
RA 1,955,326 T→G 10.0% L57L (CTT→CTG yecP → predicted methyltransferase
RA 1,969,921 G→A 22.7% L99L (CTG→TTG)  cheB ← fused chemotaxis regulator and protein‑glutamate methylesterase in two‑component regulatory system with CheA
RA 1,973,098 G→A 12.0% A436A (GCC→GCT tar ← methyl‑accepting chemotaxis protein II
RA 1,979,220 T→C 10.4% P113P (CCA→CCG flhC ← DNA‑binding transcriptional regulator with FlhD
RA 1,979,442 G→A 18.1% R39R (CGC→CGT flhC ← DNA‑binding transcriptional regulator with FlhD
RA 2,089,667 A→C 11.2% R243R (CGT→CGG yeeY ← predicted DNA‑binding transcriptional regulator
RA 2,119,282 C→T 21.9% E420E (GAG→GAA wcaK ← predicted pyruvyl transferase
RA 2,119,294 C→T 23.2% Q416Q (CAG→CAA wcaK ← predicted pyruvyl transferase
RA 2,119,312 T→C 14.7% T410T (ACA→ACG wcaK ← predicted pyruvyl transferase
RA 2,119,321 C→T 12.8% E407E (GAG→GAA wcaK ← predicted pyruvyl transferase
RA 2,122,101 C→G 11.5% S65S (TCG→TCC wzxC ← colanic acid exporter
RA 2,122,143 G→A 14.3% I51I (ATC→ATT wzxC ← colanic acid exporter
RA 2,122,146 A→G 13.2% I50I (ATT→ATC wzxC ← colanic acid exporter
RA 2,127,320 G→A 6.1% A188A (GCC→GCT wcaI ← predicted glycosyl transferase
RA 2,135,190 T→A 6.9% T115T (ACA→ACT wcaA ← predicted glycosyl transferase
MC JC 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]
RA 2,278,280 C→T 11.6% R256R (CGC→CGT yejB → predicted oligopeptide transporter subunit
RA 2,279,035 G→A 11.1% S143S (TCG→TCA yejE → predicted oligopeptide transporter subunit
RA 2,320,580 G→A 6.6% N52N (AAC→AAT rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
MC JC 2,320,795 Δ1,336 bp 100% insD[rcsC] insD, insC, [rcsC]
RA 2,405,233 A→G 17.2% H331H (CAT→CAC nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 2,419,932 A→G 12.2% A339A (GCA→GCG ackA → acetate kinase A and propionate kinase 2
RA 2,546,874 G→A 7.8% G84G (GGC→GGT cysW ← sulfate/thiosulfate transporter subunit
RA 2,546,906 G→A 7.5% L74L (CTG→TTG)  cysW ← sulfate/thiosulfate transporter subunit
RA 2,580,771 C→T 7.2% L221L (TTG→TTA ypfG ← hypothetical protein
RA 2,580,774 T→C 7.3% P220P (CCA→CCG ypfG ← hypothetical protein
RA 2,580,786 G→A 7.2% C216C (TGC→TGT ypfG ← hypothetical protein
RA 2,674,927 C→T 21.4% L185L (TTG→TTA yphD ← predicted sugar transporter subunit
RA 2,674,933 A→C 18.2% I183M (ATT→ATG yphD ← predicted sugar transporter subunit
RA 2,677,646 C→A 24.9% G126G (GGG→GGT yphF ← predicted sugar transporter subunit
RA 2,677,682 C→T 15.1% P114P (CCG→CCA yphF ← predicted sugar transporter subunit
RA 2,717,255 A→T 23.5% intergenic (‑71/‑136) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2
RA 2,717,266 C→T 20.5% intergenic (‑82/‑125) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2
RA 2,717,268 A→T 18.8% intergenic (‑84/‑123) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2
RA 2,717,279 T→C 17.0% intergenic (‑95/‑112) yfiF ← / → trxC predicted methyltransferase/thioredoxin 2
RA 2,721,287 T→C 5.8% intergenic (+18/‑96) yfiQ → / → pssA fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit/phosphatidylserine synthase
RA 2,737,004 A→G 15.0% T202A (ACC→GCC)  pheA → fused chorismate mutase P and prephenate dehydratase
RA 2,738,198 T→C 11.3% S176S (TCA→TCG tyrA ← fused chorismate mutase T and prephenate dehydrogenase
RA 2,738,210 G→A 10.5% V172V (GTC→GTT tyrA ← fused chorismate mutase T and prephenate dehydrogenase
RA 2,805,127 C→A 12.8% T154T (ACC→ACA proW → glycine betaine transporter subunit
RA 2,805,151 C→A 9.4% I162I (ATC→ATA proW → glycine betaine transporter subunit
RA 2,809,581 G→A 94.7% G52G (GGG→GGA mprA → DNA‑binding transcriptional regulator
RA 2,819,255 A→G 5.9% T471T (ACT→ACC alaS ← alanyl‑tRNA synthetase
RA 2,852,710 G→T 10.8% L253L (CTG→CTT hypE → carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein
RA 2,866,110 T→A 100% intergenic (‑20/+160) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein
RA 2,909,021 G→A 13.8% N107N (AAC→AAT mazG ← nucleoside triphosphate pyrophosphohydrolase
RA 2,953,553 C→T 10.1% V369V (GTG→GTA recB ← exonuclease V (RecBCD complex), beta subunit
RA 2,953,559 A→G 9.2% G367G (GGT→GGC recB ← exonuclease V (RecBCD complex), beta subunit
RA 2,953,568 G→A 7.7% S364S (AGC→AGT recB ← exonuclease V (RecBCD complex), beta subunit
RA 2,955,260 C→T 8.9% V761I (GTA→ATA)  ptr ← protease III
RA 2,955,291 G→A 9.5% A750A (GCC→GCT ptr ← protease III
RA 2,982,097 A→G 9.8% H228H (CAT→CAC kduI ← predicted 5‑keto 4‑deoxyuronate isomerase
RA 2,982,115 C→T 10.1% Q222Q (CAG→CAA kduI ← predicted 5‑keto 4‑deoxyuronate isomerase
RA 3,013,011 C→T 7.0% D235N (GAC→AAC)  yqeC ← conserved hypothetical protein
RA 3,020,886 A→G 7.9% E305E (GAA→GAG xdhD → fused predicted xanthine/hypoxanthine oxidase, molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,035,613 G→A 9.1% P384S (CCG→TCG)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific
RA 3,085,574 C→T 12.7% N71N (AAC→AAT metK → methionine adenosyltransferase 1
RA 3,115,678 A→T 8.3% R697R (CGT→CGA yghJ ← predicted inner membrane lipoprotein
RA 3,130,490 A→G 9.4% F192S (TTT→TCT)  yghQ ← predicted inner membrane protein
RA 3,170,237 C→T 13.3% R366R (CGC→CGT qseC → sensory histidine kinase in two‑component regulatory system with QseB
RA 3,177,041 T→C 10.3% L91L (TTG→CTG)  tolC → transport channel
RA 3,237,592 G→A 13.2% V119V (GTG→GTA alx → predicted inner membrane protein, part of terminus
RA 3,241,035 G→A 6.3% C312C (TGC→TGT uxaA ← altronate hydrolase
MC JC 3,261,134 Δ1,199 bp 100% insH[tdcD] insH, [tdcD]
RA 3,264,126 T→C 6.0% E249E (GAA→GAG tdcC ← L‑threonine/L‑serine transporter
RA 3,285,692 A→G 17.6% G120G (GGA→GGG agaD → N‑acetylgalactosamine‑specific enzyme IID component of PTS
RA 3,285,704 A→G 21.4% S124S (TCA→TCG agaD → N‑acetylgalactosamine‑specific enzyme IID component of PTS
RA 3,285,713 C→T 24.2% S127S (AGC→AGT agaD → N‑acetylgalactosamine‑specific enzyme IID component of PTS
RA 3,331,382 G→T 21.4% R76R (CGC→CGA obgE ← GTPase involved in cell partioning and DNA repair
RA 3,331,391 G→A 27.2% G73G (GGC→GGT obgE ← GTPase involved in cell partioning and DNA repair
RA 3,331,394 A→G 25.0% N72N (AAT→AAC obgE ← GTPase involved in cell partioning and DNA repair
RA 3,334,786 Δ1 bp 5.8% coding (23/279 nt) sfsB → DNA‑binding transcriptional regulator
RA 3,400,385 A→G 6.5% S186S (TCT→TCC mreB ← cell wall structural complex MreBCD, actin‑like component MreB
RA 3,400,403 A→G 6.4% G180G (GGT→GGC mreB ← cell wall structural complex MreBCD, actin‑like component MreB
RA 3,422,503 A→G 7.4% V71V (GTA→GTG yhdZ → predicted amino‑acid transporter subunit
RA 3,440,923 T→C 19.2% S114S (AGT→AGC thiC → thiamin (pyrimidine moiety) biosynthesis protein
RA 3,448,059 T→C 10.0% P1091P (CCA→CCG rpoC ← RNA polymerase, beta prime subunit
RA 3,452,623 G→A 18.0% G938G (GGC→GGT rpoB ← RNA polymerase, beta subunit
RA 3,452,626 A→C 25.5% D937E (GAT→GAG rpoB ← RNA polymerase, beta subunit
RA 3,521,980 C→T 6.0% G293G (GGC→GGT glpX → fructose 1,6‑bisphosphatase II
RA 3,522,004 G→C 6.8% A301A (GCG→GCC glpX → fructose 1,6‑bisphosphatase II
RA 3,522,007 T→C 6.7% T302T (ACT→ACC glpX → fructose 1,6‑bisphosphatase II
RA 3,534,326 A→T 7.0% T222T (ACT→ACA kdgT ← 2‑keto‑3‑deoxy‑D‑gluconate transporter
RA 3,534,338 C→T 8.9% V218V (GTG→GTA kdgT ← 2‑keto‑3‑deoxy‑D‑gluconate transporter
RA 3,578,721 T→C 8.6% T25T (ACT→ACC glnA → glutamine synthetase
RA 3,603,753 T→C 7.8% H146R (CAT→CGT) ‡ yigZ ← predicted elongation factor
RA 3,603,754 G→T 7.6% H146N (CAT→AAT) ‡ yigZ ← predicted elongation factor
RA 3,657,703 T→C 7.8% V126V (GTA→GTG wzyE ← predicted Wzy protein involved in ECA polysaccharide chain elongation
RA 3,657,709 C→A 8.9% A124A (GCG→GCT wzyE ← predicted Wzy protein involved in ECA polysaccharide chain elongation
RA 3,657,715 A→G 8.8% G122G (GGT→GGC wzyE ← predicted Wzy protein involved in ECA polysaccharide chain elongation
RA 3,672,802 A→G 6.8% A117A (GCA→GCG gpp → guanosine pentaphosphatase/exopolyphosphatase
RA 3,674,367 T→C 6.4% E546E (GAA→GAG rep ← DNA helicase and single‑stranded DNA‑dependent ATPase
RA 3,714,841 T→C 8.6% G70G (GGT→GGC atpB → F0 sector of membrane‑bound ATP synthase, subunit a
RA 3,716,825 C→A 9.1% intergenic (+12/‑1) atpH → / → atpA F1 sector of membrane‑bound ATP synthase, delta subunit/F1 sector of membrane‑bound ATP synthase, alpha subunit
MC JC 3,742,938 Δ1,336 bp 100% insDinsC insD, insC
MC JC 3,746,913 Δ1,199 bp 100% [tnaB][tnaB] [tnaB], insH, [tnaB]
MC JC 3,750,593 Δ1,199 bp 100% insH insH
RA 3,800,283 C→G 5.6% intergenic (‑81/‑141) yicS ← / → nlpA hypothetical protein/cytoplasmic membrane lipoprotein‑28
RA 3,800,285 C→T 5.7% intergenic (‑83/‑139) yicS ← / → nlpA hypothetical protein/cytoplasmic membrane lipoprotein‑28
RA 3,800,287 C→T 5.7% intergenic (‑85/‑137) yicS ← / → nlpA hypothetical protein/cytoplasmic membrane lipoprotein‑28
RA 3,804,808 C→A 6.7% G107G (GGC→GGA yicJ → predicted transporter
RA 3,804,817 C→T 6.5% I110I (ATC→ATT yicJ → predicted transporter
RA 3,805,429 C→T 8.6% P314P (CCC→CCT yicJ → predicted transporter
RA 3,805,447 T→C 8.0% T320T (ACT→ACC yicJ → predicted transporter
RA 3,824,280 T→C 100% pseudogene (413/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,326 T→C 100% pseudogene (459/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,362 A→T 100% pseudogene (495/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,368 G→A 100% pseudogene (501/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,374 C→T 100% pseudogene (507/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,380 2 bp→AA 100% pseudogene (513‑514/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,410 T→A 100% pseudogene (543/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,514 T→C 100% pseudogene (647/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,524 T→G 100% pseudogene (657/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,824,533:1 +G 100% pseudogene (666/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH
RA 3,858,954 T→C 6.9% T83T (ACA→ACG yibK ← predicted rRNA methylase
RA 3,924,649 T→C 7.0% S191S (TCT→TCC bisC → biotin sulfoxide reductase
RA 3,930,256 T→A 5.8% A105A (GCT→GCA eptB → predicted metal dependent hydrolase
RA 3,946,443 T→C 10.2% D421D (GAT→GAC bcsA → cellulose synthase, catalytic subunit
RA 3,964,464 C→A 10.2% A390S (GCG→TCG)  yhjE ← predicted transporter
RA 3,977,925 T→C 7.5% L693L (CTA→CTG mdtF ← multidrug transporter, RpoS‑dependent
RA 3,977,928 T→C 8.5% K692K (AAA→AAG mdtF ← multidrug transporter, RpoS‑dependent
RA 3,993,744 T→G 9.2% V125V (GTA→GTC gor ← glutathione oxidoreductase
RA 3,993,747 C→T 9.1% E124E (GAG→GAA gor ← glutathione oxidoreductase
RA 3,993,750 C→A 9.1% L123L (CTG→CTT) ‡ gor ← glutathione oxidoreductase
RA 3,993,752 G→T 8.0% L123M (CTG→ATG) ‡ gor ← glutathione oxidoreductase
RA 3,993,753 C→G 7.9% T122T (ACG→ACC gor ← glutathione oxidoreductase
RA 4,039,152 T→C 9.8% Y274Y (TAT→TAC ftsX → predicted transporter subunit
RA 4,041,961 C→T 6.5% intergenic (+99/+89) livJ → / ← yhhK leucine/isoleucine/valine transporter subunit/conserved hypothetical protein
RA 4,041,983 A→G 7.1% intergenic (+121/+67) livJ → / ← yhhK leucine/isoleucine/valine transporter subunit/conserved hypothetical protein
RA 4,077,892 G→A 16.2% R171R (CGC→CGT glpD ← sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
RA 4,077,898 C→A 14.9% R169R (CGG→CGT glpD ← sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
RA 4,077,913 G→A 15.7% G164G (GGC→GGT glpD ← sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
RA 4,080,396 T→C 10.2% I195I (ATT→ATC glpR → DNA‑binding transcriptional repressor
RA 4,080,432 T→C 8.5% D207D (GAT→GAC glpR → DNA‑binding transcriptional repressor
RA 4,080,438 G→A 5.9% S209S (TCG→TCA glpR → DNA‑binding transcriptional repressor
RA 4,080,456 G→A 6.1% A215A (GCG→GCA glpR → DNA‑binding transcriptional repressor
RA 4,095,396 G→T 16.1% S47S (TCC→TCA gntX ← gluconate periplasmic binding protein with phosphoribosyltransferase domain, GNT I system
RA 4,096,796 G→A 6.4% S152S (TCC→TCT yhgA ← predicted transposase
RA 4,110,393 A→G 8.2% N84N (AAT→AAC hslO ← heat shock protein Hsp33
RA 4,174,520 T→G 8.6% Y119S (TAT→TCT)  gspO ← bifunctional prepilin leader peptidase and methylase
RA 4,217,050 A→G 11.1% A75A (GCA→GCG yjaA → conserved hypothetical protein
RA 4,258,286 T→A 8.8% T591T (ACA→ACT plsB ← glycerol‑3‑phosphate O‑acyltransferase
MC JC 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]
RA 4,317,099 A→C 9.5% V191V (GTT→GTG rpiR ← DNA‑binding transcriptional repressor
RA 4,317,126 G→A 6.4% S182S (TCC→TCT rpiR ← DNA‑binding transcriptional repressor
RA 4,347,163 A→G 10.0% P191P (CCA→CCG melA → alpha‑galactosidase, NAD(P)‑binding
RA 4,371,273 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,375,910 T→C 8.2% N68N (AAT→AAC groL → Cpn60 chaperonin GroEL, large subunit of GroESL
RA 4,379,696 C→T 11.7% R215H (CGT→CAT)  yjeK ← predicted lysine aminomutase
RA 4,379,698 G→T 11.5% A214A (GCC→GCA) ‡ yjeK ← predicted lysine aminomutase
RA 4,379,700 C→A 11.0% A214S (GCC→TCC) ‡ yjeK ← predicted lysine aminomutase
RA 4,379,706 A→G 12.5% C212R (TGC→CGC)  yjeK ← predicted lysine aminomutase
RA 4,379,719 C→A 13.6% E207D (GAG→GAT yjeK ← predicted lysine aminomutase
RA 4,379,722 G→T 14.2% T206T (ACC→ACA yjeK ← predicted lysine aminomutase
RA 4,379,728 A→G 13.6% R204R (CGT→CGC yjeK ← predicted lysine aminomutase
RA 4,436,156 A→G 6.6% I170I (ATT→ATC ytfE ← predicted regulator of cell morphogenesis and cell wall metabolism
RA 4,437,511 G→A 7.8% L79L (CTC→CTT ytfF ← predicted inner membrane protein
RA 4,437,526 A→G 7.6% T74T (ACT→ACC ytfF ← predicted inner membrane protein
RA 4,539,890 T→C 5.6% M65T (ATG→ACG)  yjhR → predicted frameshift suppressor
RA 4,563,589 T→A 100% K207M (AAG→ATG)  iadA ← isoaspartyl dipeptidase
RA 4,563,924 C→T 14.3% A95A (GCG→GCA iadA ← isoaspartyl dipeptidase
RA 4,563,936 G→A 16.6% R91R (CGC→CGT iadA ← isoaspartyl dipeptidase
RA 4,563,944 G→A 12.8% L89L (CTA→TTA)  iadA ← isoaspartyl dipeptidase
RA 4,597,394 C→T 9.0% R352R (CGC→CGT tsr → methyl‑accepting chemotaxis protein I, serine sensor receptor

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 164566 164612 47 5 [2] [4] 5 hrpB/mrcB predicted ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
* * ÷ W3110S.gb 422766 422801 36 7 [4] [3] 5 malZ maltodextrin glucosidase
* * ÷ W3110S.gb 511881 511892 12 5 [1] [2] 6 ybaT predicted transporter
* * ÷ W3110S.gb 573868–575008 575761 754–1894 5 [4] [4] 7 insH–[nmpC] insH,[nmpC]
* * ÷ W3110S.gb 575791 575999 209 7 [3] [1] 104 nmpC DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
* * ÷ W3110S.gb 598781 598816 36 5 [2] [3] 5 cusA copper/silver efflux system, membrane component
* * ÷ W3110S.gb 721525 721540 16 5 [3] [4] 6 kdpE DNA‑binding response regulator in two‑component regulatory system with KdpD
* * ÷ W3110S.gb 907980 908012 33 6 [4] [4] 8 ybjT conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
* * ÷ W3110S.gb 1421314 1421332 19 5 [4] [4] 9 ydaG/racR hypothetical protein/predicted DNA‑binding transcriptional regulator
* * ÷ W3110S.gb 2041777 2041799 23 6 [4] [4] 5 yedY predicted reductase
* * ÷ W3110S.gb 2296385 2296398 14 6 [3] [2] 5 ccmF heme lyase, CcmF subunit
* * ÷ W3110S.gb 2569117 2569136 20 5 [3] [4] 6 eutE predicted aldehyde dehydrogenase, ethanolamine utilization protein
* * ÷ W3110S.gb 2827391 2827408 18 5 [4] [2] 5 gutM DNA‑binding transcriptional activator
* * ÷ W3110S.gb 4213635–4216697 4216697 1–3063 5 [4] [3] 6 [rrlE]–[rrfE] [rrlE],[rrfE]
* * ÷ W3110S.gb 4401133 4401186 54 5 [3] [4] 5 amiB N‑acetylmuramoyl‑l‑alanine amidase II

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb 70061 =50 (1.250)620 (19.940)
+CGCGTACAACT
80/78 NT 93.4% intergenic (‑13/‑326) araB/araC L‑ribulokinase/DNA‑binding transcriptional dual regulator
?W3110S.gb 236044 = 63 (1.580)intergenic (‑42/‑23) rnhA/dnaQ ribonuclease HI, degrades RNA of DNA‑RNA hybrids/DNA polymerase III epsilon subunit
* ? W3110S.gb = 7126546 (1.150)267 (9.300)
+AGGGTCCCTAAGTC
66/72 NT 92.5% coding (879/879 nt) araC DNA‑binding transcriptional dual regulator
?W3110S.gb 3464879 = 14 (0.350)intergenic (‑255/+46) murB/rrfB UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding/5S ribosomal RNA
* ? W3110S.gb 205097 =34 (0.850)65 (2.200)
+TTATAACATTTTA
20/74 NT 65.1% intergenic (+8/‑29) rnhB/dnaE ribonuclease HII, degrades RNA of DNA‑RNA hybrids/DNA polymerase III alpha subunit
?W3110S.gb 4139149 = 60 (1.510)coding (2/1023 nt) frlB fructoselysine‑6‑P‑deglycase
* ? W3110S.gb = 23055647 (1.180)14 (0.440)
+CTCCTCAGCC
8/80 NT 34.3% coding (326/915 nt) yafC predicted DNA‑binding transcriptional regulator
?W3110S.gb 1313528 = 20 (0.500)intergenic (+6/+34) tonB/yciA membrane spanning protein in TonB‑ExbB‑ExbD complex/predicted hydrolase
* ? W3110S.gb = 23679828 (0.700)254 (13.850)
+27 bp
50/46 NT 94.2% coding (732/732 nt) dnaQ DNA polymerase III epsilon subunit
?W3110S.gb 4124504 = 40 (1.000)intergenic (+106/‑1) damX/dam hypothetical protein/DNA adenine methylase
* ? W3110S.gb = 274373NA (NA)51 (1.280) 24/100 NT 98.1% intergenic (‑32/‑176) insH/mmuP IS5 transposase and trans‑activator/predicted S‑methylmethionine transporter
?W3110S.gb 576000 = 1 (0.030)pseudogene (109/1128 nt) nmpC DLP12 prophage region; ECK0544:JW5078:b0553; truncated outer membrane porin
* ? W3110S.gb = 315707NA (NA)69 (1.730) 31/100 NT 100% coding (654/687 nt) ykgA predicted DNA‑binding transcriptional regulator
?W3110S.gb 576911 = 0 (0.000)coding (76/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 566000 =NA (NA)38 (0.950) 26/100 NT 100% intergenic (+90/‑56) ybcD/insE DLP12 prophage region; ECK0532:JW0527:b4508; predicted replication protein fragment/IS3 element protein InsE
?W3110S.gb = 576913 0 (0.000)coding (78/498 nt) ybcS predicted lysozyme
* ? W3110S.gb 713380 =28 (0.700)486 (13.250) 88/92 NT 94.6% intergenic (‑156/‑29) ybfF/seqA conserved hypothetical protein/regulatory protein for replication initiation
?W3110S.gb = 4125340 30 (0.820)coding (836/837 nt) dam DNA adenine methylase
* ? W3110S.gb = 71395456 (1.400)755 (18.940) 95/100 NT 92.0% coding (546/546 nt) seqA regulatory protein for replication initiation
?W3110S.gb 2809398 = 75 (1.880)intergenic (+63/‑28) ygaH/mprA predicted inner membrane protein/DNA‑binding transcriptional regulator
* ? W3110S.gb 1209367 =11 (0.280)15 (0.550) 11/68 NT 66.0% coding (290/630 nt) ycfK hypothetical protein
?W3110S.gb 1211196 = 8 (0.300)pseudogene (37/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 120938211 (0.280)17 (0.630) 12/68 NT 68.7% coding (305/630 nt) ycfK hypothetical protein
?W3110S.gb = 1211179 8 (0.300)pseudogene (54/537 nt) stfE ECK1143:JW5172:b1157; e14 prophage region; predicted side tail fiber protein fragment
* ? W3110S.gb = 166893319 (0.480)3 (0.080) 3/90 NT 12.7% coding (1/696 nt) ynfK predicted dethiobiotin synthetase
?W3110S.gb = 1668949 24 (0.670)intergenic (‑16/+109) ynfK/dgsA predicted dethiobiotin synthetase/DNA‑binding transcriptional repressor
* ? W3110S.gb 2018571 =3 (0.080)40 (1.070) 23/94 NT 93.4% coding (567/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb 2519723 = NA (NA)intergenic (+33/‑54) nupC/insL nucleoside (except guanosine) transporter/predicted transposase
* ? W3110S.gb = 20185834 (0.100)33 (0.860) 21/96 NT 89.6% coding (579/687 nt) fliH flagellar biosynthesis protein
?W3110S.gb = 2521059 NA (NA)intergenic (+170/+30) insL/yfeA predicted transposase/predicted diguanylate cyclase
* ? W3110S.gb = 210729164 (1.610)128 (3.280)
+A
24/98 NT 71.3% coding (904/993 nt) wbbI conserved hypothetical protein
?W3110S.gb = 2809956 41 (1.030)coding (531/531 nt) mprA DNA‑binding transcriptional regulator
* ? W3110S.gb 2172308 =NA (NA)54 (1.380) 36/98 NT 100% pseudogene (1563/2041 nt) gatR DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
?W3110S.gb = 3920281 0 (0.000)coding (88/213 nt) cspA major cold shock protein
* ? W3110S.gb 2172309 =NA (NA)56 (1.460) 35/96 NT 100% pseudogene (1562/2041 nt) gatR DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
?W3110S.gb 3932930 = 0 (0.000)coding (219/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 2173564NA (NA)76 (1.950) 41/98 NT 100% pseudogene (307/2041 nt)
pseudogene (307/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb 3920278 = 0 (0.000)coding (91/213 nt) cspA major cold shock protein
* ? W3110S.gb = 2173565NA (NA)47 (1.180) 23/100 NT 100% pseudogene (306/2041 nt)
pseudogene (306/339 nt)
gatR
gatR
DNA‑binding transcriptional regulator; ECK2083:JW5340+JW2074:b4498
DNA‑binding transcriptional regulator, C‑ter fragment; ECK2083:JW2074:b2090
?W3110S.gb = 3932933 0 (0.000)coding (222/1608 nt) dppA dipeptide transporter
* ? W3110S.gb = 2412225NA (NA)63 (1.580) 36/100 NT 94.0% intergenic (‑55/‑782) insA/yfbQ IS1 element protein/predicted aminotransferase
?W3110S.gb = 3748807 4 (0.100)coding (176/984 nt) tnaB tryptophan transporter of low affinity
* ? W3110S.gb 2549829 =23 (0.580)15 (0.450)
+CAACTAAA
3/84 NT 35.1% coding (241/792 nt) ucpA predicted oxidoredutase, sulfate metabolism protein
?W3110S.gb = 3452629 43 (1.080)coding (2808/4029 nt) rpoB RNA polymerase, beta subunit
* ? W3110S.gb 2691202 =14 (0.350)7 (0.210)
+GTCAGTTC
3/84 NT 19.2% coding (2998/3888 nt) purL phosphoribosylformyl‑glycineamide synthetase
?W3110S.gb 2980369 = 56 (1.400)coding (470/1419 nt) araE arabinose transporter
* ? W3110S.gb 3748799 =9 (0.230)65 (1.630) 38/100 NT 87.8% coding (184/984 nt) tnaB tryptophan transporter of low affinity
?W3110S.gb 4056222 = NA (NA)intergenic (‑564/+15) yrhB/insB hypothetical protein/IS1 transposase InsAB'
* ? W3110S.gb 4547364 =40 (1.000)8 (0.240) 7/82 NT 19.3% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb 4547678 = 34 (1.040)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
* ? W3110S.gb = 454737245 (1.130)6 (0.180) 5/82 NT 14.5% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin
?W3110S.gb = 4547668 34 (1.040)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin