breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 17,860 C→T 8.5% N239N (AAC→AAT dacA → D‑alanyl‑D‑alanine carboxypeptidase
RA 132,186 A→G 94.7% D560G (GAC→GGC)  fusA → elongation factor G
RA 237,732 T→C 5.0% V56A (GTT→GCT)  EO946_RS01345 → YbeF family protein
RA 240,198 G→T 12.0% Q112K (CAA→AAA)  EO946_RS01360 ← hypothetical protein
RA 296,608 C→G 6.0% A201A (GCC→GCG EO946_RS01660 → DUF4901 domain‑containing protein
RA 344,548 T→C 5.2% Y132Y (TAT→TAC putR → proline utilization transcriptional regulator PutR
RA 344,557 T→C 5.2% S135S (TCT→TCC putR → proline utilization transcriptional regulator PutR
RA 344,560 T→C 5.2% F136F (TTT→TTC putR → proline utilization transcriptional regulator PutR
RA 344,593 G→A 5.1% K147K (AAG→AAA putR → proline utilization transcriptional regulator PutR
RA 344,594 C→T 5.0% L148L (CTG→TTG)  putR → proline utilization transcriptional regulator PutR
RA 344,599 G→A 5.1% K149K (AAG→AAA putR → proline utilization transcriptional regulator PutR
RA 417,261 T→A 6.7% L58L (CTA→CTT EO946_RS02145 ← hypothetical protein
RA 417,267 G→T 5.3% F56L (TTC→TTA EO946_RS02145 ← hypothetical protein
RA 438,588 C→G 5.8% L49L (CTG→CTC gabR ← transcriptional regulator GabR
RA 438,591 C→A 5.8% K48N (AAG→AAT gabR ← transcriptional regulator GabR
RA 438,594 C→T 5.6% E47E (GAG→GAA gabR ← transcriptional regulator GabR
RA 438,597 G→A 5.3% A46A (GCC→GCT gabR ← transcriptional regulator GabR
RA 480,215 T→A 5.5% P116P (CCT→CCA EO946_RS02465 → glycosyltransferase family 2 protein
RA 480,218 C→T 5.7% N117N (AAC→AAT EO946_RS02465 → glycosyltransferase family 2 protein
RA 480,221 C→A 5.7% A118A (GCC→GCA EO946_RS02465 → glycosyltransferase family 2 protein
RA 480,230 C→A 5.7% G121G (GGC→GGA EO946_RS02465 → glycosyltransferase family 2 protein
RA 480,245 G→T 5.4% L126L (CTG→CTT EO946_RS02465 → glycosyltransferase family 2 protein
RA 480,287 A→G 6.0% V140V (GTA→GTG EO946_RS02465 → glycosyltransferase family 2 protein
RA 506,292 G→C 5.6% V277V (GTG→GTC EO946_RS02595 → cation diffusion facilitator family transporter
RA 523,388 G→A 5.3% A228A (GCG→GCA EO946_RS02705 → RNA‑binding transcriptional accessory protein
RA 523,472 G→A 5.2% Q256Q (CAG→CAA EO946_RS02705 → RNA‑binding transcriptional accessory protein
RA 523,484 C→T 5.2% N260N (AAC→AAT EO946_RS02705 → RNA‑binding transcriptional accessory protein
RA 607,359 A→C 8.3% T68P (ACC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,638 G→C 12.0% A161P (GCC→CCC)  EO946_RS03185 → collagen‑like protein
RA 607,688 A→G 10.0% G177G (GGA→GGG EO946_RS03185 → collagen‑like protein
RA 607,694 C→T 16.4% T179T (ACC→ACT EO946_RS03185 → collagen‑like protein
RA 607,700 A→C 30.6% E181D (GAA→GAC EO946_RS03185 → collagen‑like protein
RA 607,702 C→T 19.6% A182V (GCC→GTC)  EO946_RS03185 → collagen‑like protein
RA 607,838 A→G 18.1% T227T (ACA→ACG EO946_RS03185 → collagen‑like protein
RA 607,847 G→A 12.2% T230T (ACG→ACA EO946_RS03185 → collagen‑like protein
RA 607,853 C→T 11.3% A232A (GCC→GCT EO946_RS03185 → collagen‑like protein
RA 607,856 A→T 12.0% T233T (ACA→ACT EO946_RS03185 → collagen‑like protein
RA 704,348 Δ1 bp 100% intergenic (+170/‑14) EO946_RS03650 → / → EO946_RS03655 CamS family sex pheromone protein/phosphotransferase enzyme family protein
RA 751,369 A→T 5.8% L187L (CTA→CTT EO946_RS03865 → YesL family protein
RA 751,373 A→T 5.7% M189L (ATG→TTG)  EO946_RS03865 → YesL family protein
RA 751,390 C→T 5.2% L194L (CTC→CTT EO946_RS03865 → YesL family protein
RA 751,393 A→G 5.2% R195R (AGA→AGG EO946_RS03865 → YesL family protein
RA 765,082 T→C 5.2% M265T (ATG→ACG)  lplD → alpha‑galacturonidase LplD
RA 765,087 A→G 5.3% S267G (AGC→GGC)  lplD → alpha‑galacturonidase LplD
RA 767,723 A→C 5.4% I81S (ATT→AGT)  EO946_RS03930 ← hypothetical protein
RA 779,147 C→T 5.1% I40I (ATC→ATT EO946_RS03975 → glucose‑1‑phosphate cytidylyltransferase
RA 828,787 G→A 100% E77K (GAA→AAA)  treC → alpha,alpha‑phosphotrehalase
RA 892,039 T→C 6.0% L230L (CTT→CTC EO946_RS04510 → iron ABC transporter permease
RA 892,051 G→A 5.8% T234T (ACG→ACA EO946_RS04510 → iron ABC transporter permease
RA 892,896 A→G 5.4% P183P (CCA→CCG EO946_RS04515 → iron ABC transporter permease
RA 892,903 A→C 5.8% R186R (AGA→CGA)  EO946_RS04515 → iron ABC transporter permease
RA 892,908 G→A 5.2% A187A (GCG→GCA EO946_RS04515 → iron ABC transporter permease
RA 892,929 T→C 6.7% I194I (ATT→ATC EO946_RS04515 → iron ABC transporter permease
RA 892,932 C→T 6.5% T195T (ACC→ACT EO946_RS04515 → iron ABC transporter permease
RA 1,013,969 C→A 5.4% D70Y (GAT→TAT)  EO946_RS05230 ← polysaccharide deacetylase
RA 1,061,263 T→C 7.1% S130S (TCT→TCC hemY → protoporphyrinogen oxidase
RA 1,061,272 A→G 7.0% A133A (GCA→GCG hemY → protoporphyrinogen oxidase
RA 1,061,275 G→A 7.2% R134R (AGG→AGA hemY → protoporphyrinogen oxidase
RA 1,061,278 C→T 7.4% A135A (GCC→GCT hemY → protoporphyrinogen oxidase
RA 1,061,293 T→C 7.3% I140I (ATT→ATC hemY → protoporphyrinogen oxidase
RA 1,196,397 A→G 5.0% K110K (AAA→AAG EO946_RS06155 → GNAT family N‑acetyltransferase
RA 1,268,698 T→A 6.4% intergenic (+8/‑192) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
RA 1,268,699 G→A 6.1% intergenic (+9/‑191) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
RA 1,268,704 Δ1 bp 5.8% intergenic (+14/‑186) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
RA 1,268,707 G→T 5.7% intergenic (+17/‑183) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
RA 1,268,709 A→G 5.6% intergenic (+19/‑181) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
RA 1,268,722 T→C 6.6% intergenic (+32/‑168) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
RA 1,268,723 G→A 6.6% intergenic (+33/‑167) EO946_RS06595 → / → uxaC NAD(P)/FAD‑dependent oxidoreductase/glucuronate isomerase
RA 1,342,854 T→C 5.0% N67N (AAT→AAC hmpA → NO‑inducible flavohemoprotein
RA 1,365,757 G→A 5.0% S85S (TCG→TCA EO946_RS07135 → PepSY domain‑containing protein
RA 1,365,763 C→T 5.2% N87N (AAC→AAT EO946_RS07135 → PepSY domain‑containing protein
RA 1,397,487 T→C 5.1% V58V (GTT→GTC mtnE → methionine‑glutamine aminotransferase
RA 1,418,373 T→C 16.3% intergenic (+104/‑131) EO946_RS07425 → / → pfeT TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT
RA 1,418,376 T→C 15.8% intergenic (+107/‑128) EO946_RS07425 → / → pfeT TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT
RA 1,418,378 C→T 19.3% intergenic (+109/‑126) EO946_RS07425 → / → pfeT TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT
RA 1,418,381 A→G 20.7% intergenic (+112/‑123) EO946_RS07425 → / → pfeT TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT
RA 1,418,409 G→A 12.5% intergenic (+140/‑95) EO946_RS07425 → / → pfeT TlpA family protein disulfide reductase/metal‑transporting ATPase PfeT
RA 1,465,903 T→C 5.4% I147I (ATT→ATC EO946_RS07670 → ABC transporter ATP‑binding protein/permease
RA 1,566,216 A→G 7.5% N132D (AAT→GAT)  EO946_RS08175 → S8 family serine peptidase
RA 1,566,221 G→A 6.9% K133K (AAG→AAA EO946_RS08175 → S8 family serine peptidase
RA 1,659,112 G→A 7.1% G16S (GGC→AGC)  flgB → flagellar basal body rod protein FlgB
RA 1,684,359 T→C 5.2% D26D (GAT→GAC sigD → RNA polymerase sigma‑28 factor SigD
RA 1,704,957 A→C 6.5% Q94H (CAA→CAC truB → tRNA pseudouridine(55) synthase TruB
RA 1,704,974 T→A 6.3% V100E (GTA→GAA) ‡ truB → tRNA pseudouridine(55) synthase TruB
RA 1,704,975 A→T 6.0% V100V (GTA→GTT) ‡ truB → tRNA pseudouridine(55) synthase TruB
RA 1,704,987 G→T 6.3% V104V (GTG→GTT truB → tRNA pseudouridine(55) synthase TruB
RA 1,705,005 T→G 6.7% G110G (GGT→GGG truB → tRNA pseudouridine(55) synthase TruB
RA 1,705,023 T→G 6.8% P116P (CCT→CCG truB → tRNA pseudouridine(55) synthase TruB
RA 1,734,397 T→C 8.8% N221N (AAT→AAC EO946_RS09000 → serine hydrolase
RA 1,734,409 C→T 8.9% L225L (CTC→CTT EO946_RS09000 → serine hydrolase
RA 1,734,419 G→A 9.5% V229I (GTA→ATA) ‡ EO946_RS09000 → serine hydrolase
RA 1,734,421 A→T 8.7% V229V (GTA→GTT) ‡ EO946_RS09000 → serine hydrolase
RA 1,738,217 A→T 5.4% intergenic (+146/‑120) spoVS → / → tdh stage V sporulation protein SpoVS/L‑threonine 3‑dehydrogenase
RA 1,754,574 G→T 5.7% R254I (AGA→ATA) ‡ EO946_RS09090 → acyltransferase domain‑containing protein
RA 1,754,575 A→G 5.7% R254R (AGA→AGG) ‡ EO946_RS09090 → acyltransferase domain‑containing protein
RA 1,759,155 G→A 5.1% L176L (TTG→TTA pksG → polyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG
RA 1,759,161 G→C 5.7% G178G (GGG→GGC pksG → polyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG
RA 1,759,191 A→C 5.5% P188P (CCA→CCC pksG → polyketide biosynthesis 3‑hydroxy‑3‑methylglutaryl‑ACP synthase PksG
RA 1,761,511 C→T 6.1% T7I (ACC→ATC)  EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,517 A→T 6.0% N9I (AAT→ATT) ‡ EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,518 T→A 6.1% N9K (AAT→AAA) ‡ EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,522 G→A 6.2% A11T (GCT→ACT)  EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,539 C→T 6.2% S16S (AGC→AGT EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,540 T→C 6.1% Y17H (TAT→CAT)  EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,545 T→G 6.2% G18G (GGT→GGG EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,549 A→C 6.4% T20P (ACT→CCT)  EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,567 G→A 8.7% E26K (GAG→AAG)  EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,761,581 G→A 8.0% T30T (ACG→ACA EO946_RS09125 → non‑ribosomal peptide synthetase
RA 1,846,125 G→A 5.5% G212G (GGG→GGA EO946_RS09265 → aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
RA 1,902,254 A→G 5.6% F13F (TTT→TTC EO946_RS09605 ← hypothetical protein
RA 1,902,266 C→T 5.6% A9A (GCG→GCA EO946_RS09605 ← hypothetical protein
RA 1,902,272 A→C 5.6% L7L (CTT→CTG) ‡ EO946_RS09605 ← hypothetical protein
RA 1,902,274 G→A 5.8% L7F (CTT→TTT) ‡ EO946_RS09605 ← hypothetical protein
RA 1,968,182 C→A 16.4% L85I (CTA→ATA)  EO946_RS09780 → hypothetical protein
RA 1,995,953 T→C 7.4% I29M (ATA→ATG EO946_RS09955 ← hypothetical protein
RA 2,018,214 G→T 6.3% D283E (GAC→GAA proC ← pyrroline‑5‑carboxylate reductase
RA 2,018,250 A→T 5.0% I271I (ATT→ATA proC ← pyrroline‑5‑carboxylate reductase
RA 2,018,253 C→T 5.6% A270A (GCG→GCA proC ← pyrroline‑5‑carboxylate reductase
RA 2,117,175 G→T 5.1% R535R (CGG→AGG)  EO946_RS10610 ← nitric oxide reductase activation protein NorD
RA 2,117,179 C→T 5.0% A533A (GCG→GCA EO946_RS10610 ← nitric oxide reductase activation protein NorD
RA 2,117,182 A→G 5.1% Y532Y (TAT→TAC EO946_RS10610 ← nitric oxide reductase activation protein NorD
RA 2,117,185 G→C 6.2% G531G (GGC→GGG EO946_RS10610 ← nitric oxide reductase activation protein NorD
RA 2,135,516 C→A 7.9% A496E (GCA→GAA)  EO946_RS10715 → sodium:solute symporter
RA 2,145,766 A→G 5.1% L309L (TTG→CTG)  EO946_RS10800 ← peptidase
RA 2,145,791 T→A 5.0% A300A (GCA→GCT EO946_RS10800 ← peptidase
RA 2,145,803 T→C 5.1% E296E (GAA→GAG EO946_RS10800 ← peptidase
RA 2,154,383 G→A 6.1% L174L (CTA→TTA)  EO946_RS10855 ← phytase
RA 2,154,419 G→A 6.2% L162L (CTG→TTG)  EO946_RS10855 ← phytase
RA 2,154,420 A→G 6.3% S161S (AGT→AGC EO946_RS10855 ← phytase
RA 2,165,568 T→G 5.1% E94D (GAA→GAC EO946_RS10925 ← hypothetical protein
RA 2,180,184 T→C 6.8% D18D (GAT→GAC sspL → small, acid‑soluble spore protein L
RA 2,180,226 A→G 7.2% E32E (GAA→GAG sspL → small, acid‑soluble spore protein L
RA 2,180,229 A→G 7.2% E33E (GAA→GAG sspL → small, acid‑soluble spore protein L
RA 2,180,235 C→A 5.8% A35A (GCC→GCA sspL → small, acid‑soluble spore protein L
RA 2,294,428 G→T 5.5% G32G (GGC→GGA EO946_RS11665 ← YpuI family protein
RA 2,301,730 C→G 5.3% G157G (GGG→GGC lepB ← signal peptidase I
RA 2,301,736 T→A 5.5% R155R (CGA→CGT lepB ← signal peptidase I
RA 2,601,897 T→A 5.2% S37S (TCA→TCT EO946_RS13465 ← penicillin‑binding protein 2
RA 2,611,703 T→G 5.1% intergenic (‑322/+12) alaS ← / ← EO946_RS13520 alanine‑‑tRNA ligase/AI‑2E family transporter
RA 2,611,711 A→G 5.4% intergenic (‑330/+4) alaS ← / ← EO946_RS13520 alanine‑‑tRNA ligase/AI‑2E family transporter
RA 2,632,070 G→A 6.9% V53V (GTC→GTT EO946_RS13615 ← adenine phosphoribosyltransferase
RA 2,706,418 G→T 6.4% intergenic (‑539/‑278) ilvB ← / → EO946_RS13980 acetolactate synthase large subunit/hypothetical protein
RA 2,711,930 G→T 7.9% T127T (ACC→ACA EO946_RS14015 ← ribonuclease PH
RA 2,711,936 T→A 7.9% T125T (ACA→ACT EO946_RS14015 ← ribonuclease PH
RA 2,711,939 C→T 7.9% G124G (GGG→GGA EO946_RS14015 ← ribonuclease PH
RA 2,711,942 G→T 7.4% G123G (GGC→GGA EO946_RS14015 ← ribonuclease PH
RA 2,711,951 C→T 8.0% Q120Q (CAG→CAA EO946_RS14015 ← ribonuclease PH
RA 2,720,740 G→A 5.2% C143C (TGC→TGT EO946_RS14065 ← aspartate kinase
RA 2,720,770 G→A 5.3% V133V (GTC→GTT EO946_RS14065 ← aspartate kinase
RA 2,720,790 G→T 5.1% Q127K (CAA→AAA)  EO946_RS14065 ← aspartate kinase
RA 2,720,791 C→T 5.5% E126E (GAG→GAA EO946_RS14065 ← aspartate kinase
RA 2,720,830 G→A 5.2% I113I (ATC→ATT EO946_RS14065 ← aspartate kinase
RA 2,720,854 T→C 5.5% E105E (GAA→GAG EO946_RS14065 ← aspartate kinase
RA 2,794,219 A→C 5.1% intergenic (‑101/+64) icd ← / ← citZ NADP‑dependent isocitrate dehydrogenase/citrate synthase
RA 2,827,922 T→C 7.0% R329R (CGA→CGG thiI ← tRNA 4‑thiouridine(8) synthase ThiI
RA 2,827,949 A→G 8.4% D320D (GAT→GAC thiI ← tRNA 4‑thiouridine(8) synthase ThiI
RA 2,827,963 A→G 7.8% L316L (TTA→CTA)  thiI ← tRNA 4‑thiouridine(8) synthase ThiI
RA 2,837,041 A→G 5.9% Y126Y (TAT→TAC EO946_RS14635 ← diguanylate cyclase
RA 2,837,054 A→T 7.1% I122N (ATC→AAC)  EO946_RS14635 ← diguanylate cyclase
RA 2,901,671 A→C 8.7% K411T (AAA→ACA)  EO946_RS14945 → extracellular solute‑binding protein
RA 2,917,007 A→G 5.6% D220D (GAT→GAC ytrF ← ABC transporter permease YtrF
RA 2,917,019 C→T 5.1% K216K (AAG→AAA ytrF ← ABC transporter permease YtrF
RA 2,928,089 T→A 5.2% intergenic (‑49/+91) alaP ← / ← asnB alanine permease AlaP/asparagine synthase (glutamine‑hydrolyzing)
RA 2,928,096 G→A 5.7% intergenic (‑56/+84) alaP ← / ← asnB alanine permease AlaP/asparagine synthase (glutamine‑hydrolyzing)
RA 2,928,097 T→C 5.6% intergenic (‑57/+83) alaP ← / ← asnB alanine permease AlaP/asparagine synthase (glutamine‑hydrolyzing)
RA 2,939,941 C→T 5.1% L144L (CTG→CTA EO946_RS15145 ← DNA starvation/stationary phase protection protein
RA 2,945,599 G→T 5.3% intergenic (+26/‑28) EO946_RS15185 → / → EO946_RS15190 cytochrome d ubiquinol oxidase subunit II/DUF1540 domain‑containing protein
RA 2,991,252 C→A 23.0% intergenic (+53/‑154) gbsR → / → bslA transcriptional regulator GbsR/biofilm‑surface layer protein BslA
RA 2,995,560 C→T 5.0% R10K (AGA→AAA)  lytG ← exo‑glucosaminidase LytG
RA 2,995,605 G→A 5.1% intergenic (‑17/+143) lytG ← / ← EO946_RS15510 exo‑glucosaminidase LytG/MFS transporter
RA 3,012,140 G→A 5.3% intergenic (‑108/+21) mcpA ← / ← tlpA methyl‑accepting chemotaxis protein/methyl‑accepting chemotaxis protein TlpA
RA 3,036,047 T→C 8.0% H10H (CAT→CAC kinB → sporulation sensor histidine kinase KinB
RA 3,036,077 C→T 5.9% L20L (CTC→CTT kinB → sporulation sensor histidine kinase KinB
RA 3,036,101 G→A 5.2% K28K (AAG→AAA kinB → sporulation sensor histidine kinase KinB
RA 3,036,104 G→T 5.2% P29P (CCG→CCT kinB → sporulation sensor histidine kinase KinB
RA 3,036,110 C→T 5.2% I31I (ATC→ATT kinB → sporulation sensor histidine kinase KinB
RA 3,122,377 A→G 5.3% intergenic (‑34/+134) EO946_RS16130 ← / ← cotNH homoserine dehydrogenase/spore coat‑associated protein CotNH
RA 3,122,395 C→T 5.7% intergenic (‑52/+116) EO946_RS16130 ← / ← cotNH homoserine dehydrogenase/spore coat‑associated protein CotNH
RA 3,180,566 C→T 6.1% intergenic (‑48/‑127) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
RA 3,180,581 T→C 6.4% intergenic (‑63/‑112) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
RA 3,180,614 C→T 5.4% intergenic (‑96/‑79) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
RA 3,180,654 T→C 5.8% intergenic (‑136/‑39) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
RA 3,180,663 A→T 6.0% intergenic (‑145/‑30) EO946_RS16450 ← / → EO946_RS16455 3‑hydroxyacyl‑CoA dehydrogenase/enoyl‑CoA hydratase family protein/YuzL family protein
RA 3,212,247 C→T 6.0% A129A (GCG→GCA EO946_RS16630 ← ABC transporter substrate‑binding protein
RA 3,212,268 A→G 5.3% A122A (GCT→GCC EO946_RS16630 ← ABC transporter substrate‑binding protein
RA 3,212,271 A→C 5.3% L121L (CTT→CTG EO946_RS16630 ← ABC transporter substrate‑binding protein
RA 3,247,065 C→T 100% intergenic (‑11/+110) bdbD ← / ← cadA disulfide bond formation protein BdbD/cadmium‑translocating P‑type ATPase
RA 3,285,491 T→C 5.4% I304V (ATT→GTT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,567 C→T 28.1% L278L (CTG→CTA EO946_RS16995 ← IS3 family transposase
RA 3,285,575 T→G 27.9% N276H (AAT→CAT)  EO946_RS16995 ← IS3 family transposase
RA 3,285,580 A→G 31.5% I274T (ATA→ACA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,647 C→A 31.5% V252L (GTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,285,713 G→A 22.7% L230L (CTA→TTA)  EO946_RS16995 ← IS3 family transposase
RA 3,308,421 A→G 8.8% G194G (GGT→GGC EO946_RS17110 ← glycosyl hydrolase family 18 protein
RA 3,308,465 G→T 5.8% Q180K (CAA→AAA)  EO946_RS17110 ← glycosyl hydrolase family 18 protein
RA 3,328,444 A→G 6.2% Q99Q (CAA→CAG lutP → L‑lactate permease LutP
RA 3,328,453 T→C 6.6% I102I (ATT→ATC lutP → L‑lactate permease LutP
RA 3,340,444 G→A 6.1% L66L (CTG→TTG)  epsG ← biofilm exopolysaccharide biosynthesis protein EpsG
RA 3,340,448 T→C 6.4% T64T (ACA→ACG epsG ← biofilm exopolysaccharide biosynthesis protein EpsG
RA 3,340,457 T→C 7.1% Q61Q (CAA→CAG epsG ← biofilm exopolysaccharide biosynthesis protein EpsG
RA 3,340,477 T→G 5.7% R55R (AGA→CGA)  epsG ← biofilm exopolysaccharide biosynthesis protein EpsG
RA 3,340,478 G→A 5.6% Y54Y (TAC→TAT epsG ← biofilm exopolysaccharide biosynthesis protein EpsG
RA 3,340,481 A→G 5.5% R53R (CGT→CGC epsG ← biofilm exopolysaccharide biosynthesis protein EpsG
RA 3,340,484 A→G 5.4% L52L (CTT→CTC epsG ← biofilm exopolysaccharide biosynthesis protein EpsG
RA 3,340,490 C→T 5.1% S50S (TCG→TCA epsG ← biofilm exopolysaccharide biosynthesis protein EpsG
RA 3,523,093 C→G 5.1% G143G (GGG→GGC EO946_RS18120 ← CotH kinase family protein
RA 3,523,104 A→G 5.0% L140L (TTG→CTG)  EO946_RS18120 ← CotH kinase family protein
RA 3,523,109 T→C 5.9% K138R (AAG→AGG)  EO946_RS18120 ← CotH kinase family protein
RA 3,523,124 A→T 5.4% F133Y (TTT→TAT)  EO946_RS18120 ← CotH kinase family protein
RA 3,564,437 T→A 5.0% S265S (TCT→TCA EO946_RS21110 → histidine kinase
RA 3,564,446 C→T 5.5% F268F (TTC→TTT EO946_RS21110 → histidine kinase
RA 3,668,421 A→T 5.4% M330L (ATG→TTG)  EO946_RS18875 → insulinase family protein
RA 3,668,429 T→G 5.0% L332L (CTT→CTG EO946_RS18875 → insulinase family protein
RA 3,668,450 G→T 5.4% G339G (GGG→GGT EO946_RS18875 → insulinase family protein
RA 3,668,453 T→A 5.1% L340L (CTT→CTA EO946_RS18875 → insulinase family protein
RA 3,746,846 C→G 5.4% G74G (GGG→GGC EO946_RS19275 ← galactokinase
RA 3,746,850 A→G 5.8% V73A (GTA→GCA)  EO946_RS19275 ← galactokinase
RA 3,746,856 T→C 6.3% K71R (AAA→AGA)  EO946_RS19275 ← galactokinase
RA 3,746,864 G→A 6.4% D68D (GAC→GAT EO946_RS19275 ← galactokinase
RA 3,746,884 C→G 6.2% V62L (GTT→CTT)  EO946_RS19275 ← galactokinase
RA 3,991,699 T→C 29.0% K46E (AAA→GAA)  walH ← WalRK two‑component regulatory system regulator WalH
RA 4,022,624 G→C 10.3% A616P (GCC→CCC)  EO946_RS20675 → thioredoxin domain‑containing protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP034943 2356246 2356443 198 37 [35] [35] 39 [gndA]–[EO946_RS12045] [gndA],[EO946_RS12045]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 91969NA (NA)11 (0.080) 4/250 NT NA intergenic (+131/‑39) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
?NZ_CP034943 91973 = NA (NA)intergenic (+135/‑35) EO946_RS00485/EO946_RS00490 16S ribosomal RNA/23S ribosomal RNA
* ? NZ_CP034943 607524 =89 (0.610)18 (0.140)
+GGCTGGTCCGACGTCC
6/220 NT 12.8% coding (367/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607858 191 (1.310)coding (701/1125 nt) EO946_RS03185 collagen‑like protein