breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,725 T→C D463D (GAT→GAC thrA → bifunctional aspartate kinase/homoserine dehydrogenase I
RA 28,032 T→C F247S (TTC→TCC)  rihC → ribonucleoside hydrolase RihC
RA 116,817 T→C I408V (ATC→GTC)  aroP ← aromatic amino acid transporter AroP
RA 121,596 A→G H698R (CAC→CGC)  aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA 139,901 T→G G212G (GGT→GGG yadG → ABC transporter ATP‑binding protein
RA 159,790 A→G E400G (GAG→GGG)  hrpB → ATP‑dependent helicase HrpB
RA 183,254 T→C G628G (GGA→GGG glnD ← bifunctional uridylyltransferase/uridylyl‑removing protein GlnD
RA 198,290 A→G E278G (GAA→GGA)  lpxD → UDP‑3‑O‑(3‑hydroxymyristoyl)glucosamine N‑acyltransferase
RA 204,438 A→G Q942Q (CAA→CAG dnaE → DNA polymerase III subunit alpha
RA 230,562 T→C Q236Q (CAA→CAG gloB ← hydroxyacylglutathione hydrolase
RA 232,843 T→G I97S (ATC→AGC)  dnaQ → DNA polymerase III subunit epsilon
RA 235,808 Δ1 bp pseudogene (222/279 nt) BW25113_RS25420 → ISAs1 family transposase
RA 278,554 A→G H189R (CAC→CGC)  yagE → 2‑keto‑3‑deoxygluconate aldolase
RA 302,805 T→C M747V (ATG→GTG)  ecpC ← fimbrial usher EcpC
RA 328,409 C→T P110S (CCT→TCT)  pdeL → DNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
RA 333,799 A→T G437G (GGA→GGT fdrA → acyl‑CoA synthetase FdrA
RA 375,377 (G)6→7 intergenic (‑40/+148) frmR ← / ← yaiO formaldehyde‑responsive transcriptional repressor FrmR/YaiO family outer membrane beta‑barrel protein
RA 410,572 A→T L213H (CTC→CAC)  sbcC ← exonuclease subunit SbcC
RA 411,160 T→C E17G (GAA→GGA)  sbcC ← exonuclease subunit SbcC
RA 432,061 G→A T324T (ACG→ACA
R6H (CGC→CAC) 
thiL →
pgpA →
thiamine‑phosphate kinase
phosphatidylglycerophosphatase A
RA 436,468 A→G T30T (ACT→ACC ispA ← (2E,6E)‑farnesyl diphosphate synthase
RA 471,023 G→A P63P (CCG→CCA ybaY → YbaY family lipoprotein
RA 485,717 T→C E4G (GAA→GGA)  rsmS ← pleiotropic regulatory protein RsmS
RA 497,636 T→C G353G (GGA→GGG ybaL ← Kef family K(+) transporter
RA 500,101 T→C T18A (ACC→GCC)  fsr ← fosmidomycin MFS transporter
RA 502,603 (A)8→7 intergenic (‑65/+139) ybaK ← / ← ybaP Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase YbaK/TraB/GumN family protein
RA 513,425 Δ1 bp coding (311/855 nt) cnoX ← chaperedoxin
RA 527,001 T→C E226G (GAA→GGA)  allS ← HTH‑type transcriptional activator AllS
RA 541,549 Δ1 bp coding (271/1050 nt) allD ← ureidoglycolate dehydrogenase
RA 547,766 T→G A95A (GCA→GCC purK ← 5‑(carboxyamino)imidazole ribonucleotide synthase
RA 551,127 A→G E354G (GAA→GGA)  cysS → cysteine‑‑tRNA ligase
RA 559,561 (A)6→5 coding (376/633 nt) fimZ ← fimbria biosynthesis transcriptional regulator FimZ
RA 594,745 T→C D192D (GAT→GAC cusA → Cu(+)/Ag(+) efflux RND transporter permease subunit CusA
RA 643,749 T→C T427A (ACC→GCC)  citF ← citrate lyase subunit alpha
RA 683,001 T→C D68G (GAT→GGT)  gltI ← glutamate/aspartate ABC transporter substrate‑binding protein GltI
RA 688,227 A→G L203P (CTG→CCG)  ybeZ ← PhoH family protein
RA 762,108 C→T G203G (GGC→GGT mngA → PTS 2‑O‑a‑mannosyl‑D‑glycerate transporter subunit IIABC
RA 778,272 T→C S244S (AGT→AGC nadA → quinolinate synthase NadA
RA 811,694 T→C D137G (GAT→GGT)  yvcK ← uridine diphosphate‑N‑acetylglucosamine‑binding protein YvcK
RA 839,475 T→C I488V (ATC→GTC)  ybiO ← mechanosensitive channel protein
RA 843,818 Δ1 bp intergenic (‑358/+46) glnH ← / ← dps glutamine ABC transporter substrate‑binding protein GlnH/DNA starvation/stationary phase protection protein Dps
RA 847,270 A→G S262P (TCC→CCC)  opgE ← phosphoethanolamine transferase
RA 903,381 A→G S120P (TCG→CCG)  ybjT ← SDR family oxidoreductase
RA 905,181 T→C D442G (GAT→GGT)  poxB ← ubiquinone‑dependent pyruvate dehydrogenase
RA 982,464 (T)8→7 intergenic (‑26/+577) ompF ← / ← asnS porin OmpF/asparagine‑‑tRNA ligase
RA 1,080,012 A→G Y394C (TAC→TGC)  efeB → iron uptake transporter deferrochelatase/peroxidase subunit
RA 1,094,108 A→G E256G (GAA→GGA)  ghrA → glyoxylate/hydroxypyruvate reductase GhrA
RA 1,095,349 Δ1 bp coding (254/555 nt) ycdY → molecular chaperone YcdY
RA 1,097,924 A→G V25A (GTA→GCA)  csgE ← curli production assembly/transport protein CsgE
RA 1,138,424 T→C D467G (GAC→GGC)  rne ← ribonuclease E
RA 1,148,053 G→T S220I (AGC→ATC)  fabF → beta‑ketoacyl‑ACP synthase II
RA 1,239,612 A→G H124H (CAT→CAC ycgR ← flagellar brake protein
RA 1,239,827 G→T L53M (CTG→ATG)  ycgR ← flagellar brake protein
RA 1,245,651 A→G V215A (GTC→GCC)  dhaK ← dihydroxyacetone kinase subunit DhaK
RA 1,254,550 T→C intergenic (+25/+30) ychH → / ← dauA stress‑induced protein YchH/C4‑dicarboxylic acid transporter DauA
RA 1,279,258 A→G I67V (ATC→GTC)  narH → nitrate reductase subunit beta
RA 1,293,696 A→C intergenic (‑119/‑274) adhE ← / → ychE bifunctional acetaldehyde‑CoA/alcohol dehydrogenase/protein YchT
RA 1,295,255 A→C intergenic (‑100/‑184) BW25113_RS25865 ← / → oppA hypothetical protein/oligopeptide ABC transporter substrate‑binding protein OppA
RA 1,296,392 A→G A318A (GCA→GCG oppA → oligopeptide ABC transporter substrate‑binding protein OppA
RA 1,333,464 A→G intergenic (‑47/‑123) ribA ← / → pgpB GTP cyclohydrolase II/phosphatidylglycerophosphatase B
RA 1,339,195 G→A A602V (GCG→GTG)  pdeR ← cyclic di‑GMP phosphodiesterase
RA 1,351,156 T→C D71G (GAT→GGT)  sapA ← peptide ABC transporter substrate‑binding protein SapA
RA 1,357,220 A→G D74G (GAC→GGC)  puuC → aldehyde dehydrogenase PuuC
RA 1,367,676 T→C D66D (GAT→GAC ycjO → sugar ABC transporter permease
RA 1,373,311 A→C T391P (ACG→CCG)  ycjT → kojibiose phosphorylase
RA 1,391,832 C→T L71L (CTG→TTG)  ynaJ → DUF2534 family protein
RA 1,472,006 A→G G43G (GGA→GGG ynbA → CDP‑alcohol phosphatidyltransferase family protein
RA 1,480,218 T→C D967D (GAT→GAC hrpA → ATP‑dependent RNA helicase HrpA
RA 1,503,813 G→T V17F (GTT→TTT)  hicB → type II toxin‑antitoxin system antitoxin HicB
RA 1,506,265 A→G K119E (AAA→GAA)  ydcS → ABC transporter substrate‑binding protein
RA 1,528,286 G→A T2T (ACG→ACA nhoA → N‑hydroxyarylamine O‑acetyltransferase
RA 1,535,292 T→C E519G (GAA→GGA)  narZ ← nitrate reductase Z subunit alpha
RA 1,551,234 T→C I118T (ATC→ACC)  osmC → peroxiredoxin OsmC
RA 1,606,026 A→G pseudogene (125/987 nt) dgcF ← GGDEF domain‑containing protein
RA 1,612,612 A→C V185G (GTG→GGG)  marC ← NAAT family transporter MarC
RA 1,614,132 A→G D101G (GAT→GGT)  marA → MDR efflux pump AcrAB transcriptional activator MarA
RA 1,615,927 (T)5→4 coding (339/1188 nt) ydeE → efflux MFS transporter YdeE
RA 1,624,359 T→C E271G (GAA→GGA)  ydfI ← mannitol dehydrogenase family protein
RA 1,632,977 (A)8→7 intergenic (‑53/+310) cspI ← / ← rzpQ cold shock protein CspI/DUF2514 domain‑containing protein
RA 1,651,047 A→G V223A (GTC→GCC)  ynfC ← YnfC family lipoprotein
RA 1,657,277 T→C S11P (TCC→CCC)  dmsB → dimethylsulfoxide reductase subunit B
RA 1,657,602 A→G E119G (GAA→GGA)  dmsB → dimethylsulfoxide reductase subunit B
RA 1,678,966 A→G I151V (ATC→GTC)  tus → DNA replication terminus site‑binding protein
RA 1,688,059 G→A A154A (GCC→GCT uidB ← glucuronide transporter
RA 1,699,521 G→A T5T (ACG→ACA ydgK → DUF2569 domain‑containing protein
RA 1,709,042 A→G N137D (AAC→GAC)  gstA → glutathione transferase GstA
RA 1,721,950 A→C P345P (CCA→CCC nemA → N‑ethylmaleimide reductase
RA 1,740,776 T→C noncoding (4/77 nt) BW25113_RS08720 → tRNA‑Val
RA 1,741,262 A→G *102W (TGA→TGG ydhR → monooxygenase
RA 1,751,148 A→G A398A (GCA→GCG pykF → pyruvate kinase PykF
RA 1,769,167 (A)5→4 coding (225/759 nt) aroD → type I 3‑dehydroquinate dehydratase
RA 1,782,562 (T)7→8 intergenic (+27/‑130) ppsR → / → aroH posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR/3‑deoxy‑7‑phosphoheptulonate synthase AroH
RA 1,794,866 C→T A10A (GCG→GCA infC ← translation initiation factor IF‑3
RA 1,794,884 T→C G4G (GGA→GGG infC ← translation initiation factor IF‑3
RA 1,799,314 A→G intergenic (+64/‑108) espL1 → / → BW25113_RS09005 type III secretion system effector EspL1/hypothetical protein
RA 1,803,183 T→C D103G (GAT→GGT)  yniB ← YniB family protein
RA 1,840,050 T→C S390G (AGT→GGT)  topB ← DNA topoisomerase III
RA 1,876,783 A→G R205R (CGA→CGG dmlA → multifunctional D‑malate/3‑isopropylmalate/tartarate dehydrogenase
RA 1,880,952 A→G E284G (GAA→GGA)  yeaX → carnitine monooxygenase subunit YeaX
RA 1,889,775 C→T R238R (CGC→CGT pabB → aminodeoxychorismate synthase component 1
RA 1,906,562 A→G I91T (ATC→ACC)  htpX ← protease HtpX
RA 1,912,667 A→T M301L (ATG→TTG)  yebT → lipid‑binding membrane homeostasis protein YebT
RA 1,912,750 T→C D328D (GAT→GAC yebT → lipid‑binding membrane homeostasis protein YebT
RA 1,914,076 T→G G770G (GGT→GGG yebT → lipid‑binding membrane homeostasis protein YebT
RA 1,932,672 A→G E256G (GAA→GGA)  pyk → pyruvate kinase
RA 1,937,480 A→C T188P (ACC→CCC)  znuC → zinc ABC transporter ATP‑binding protein ZnuC
RA 1,937,544 T→C L209P (CTG→CCG)  znuC → zinc ABC transporter ATP‑binding protein ZnuC
RA 1,963,824 A→G V473A (GTC→GCC)  tap ← methyl‑accepting chemotaxis protein IV
RA 1,972,691 A→G intergenic (‑237/‑543) flhD ← / → uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
RA 2,052,997 T→C M111V (ATG→GTG)  yeeO ← toxic metabolite efflux MATE transporter YeeO
RA 2,117,136 A→T I289N (ATC→AAC)  cpsB ← mannose‑1‑phosphate guanyltransferase
RA 2,148,643 A→G T383A (ACC→GCC)  mdtA → multidrug efflux RND transporter subunit MdtA
RA 2,173,405 A→G E369G (GAA→GGA)  yegT → nucleoside permease
RA 2,192,420 A→G pseudogene (2468/3796 nt) BW25113_RS25680 → WGR and DUF4132 domain‑containing protein
RA 2,236,596 A→C V179G (GTG→GGG)  folE ← GTP cyclohydrolase I FolE
RA 2,241,916 A→G G32G (GGT→GGC lysP ← lysine‑specific permease
RA 2,263,024 (A)6→5 coding (692/714 nt) lpxT → Kdo(2)‑lipid A phosphotransferase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 2,413,360 A→G V202A (GTC→GCC)  yfcF ← glutathione transferase
RA 2,418,915 T→A I161F (ATC→TTC)  hisQ ← histidine ABC transporter permease HisQ
RA 2,426,064 A→G S120P (TCC→CCC)  folC ← bifunctional tetrahydrofolate synthase/dihydrofolate synthase
RA 2,432,392 A→G I322V (ATT→GTT)  flk → flagella biosynthesis regulator Flk
RA 2,435,568 Δ1 bp coding (326/2007 nt) mnmC → bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC
RA 2,446,677 T→C pseudogene (1804/2646 nt) yfcU ← fimbrial usher protein YfcU
RA 2,484,883 T→G T183P (ACG→CCG)  oxc ← oxalyl‑CoA decarboxylase
RA 2,485,462 A→G intergenic (‑33/+21) oxc ← / ← frc oxalyl‑CoA decarboxylase/formyl‑CoA transferase
RA 2,500,004 T→C G36G (GGA→GGG ypdF ← aminopeptidase
RA 2,520,662 T→C S120P (TCA→CCA)  yfeH → bile acid:sodium symporter
RA 2,557,422 T→C D28D (GAT→GAC BW25113_RS12770 → hypothetical protein
RA 2,559,856 T→C I130V (ATC→GTC)  eutA ← ethanolamine ammonia‑lyase reactivating factor EutA
RA 2,621,216 A→G I361T (ATC→ACC)  pdeF ← cyclic‑guanylate‑specific phosphodiesterase PdeF
RA 2,649,892 A→G intergenic (‑175/+3) pepB ← / ← iscX aminopeptidase PepB/Fe‑S cluster assembly protein IscX
RA 2,684,441 (A)5→4 coding (17/1428 nt) qseE ← two component system sensor histidine kinase QseE/GlrK
RA 2,695,236 T→C D198G (GAC→GGC)  recO ← DNA repair protein RecO
RA 2,734,303 A→G T69T (ACT→ACC aroF ← 3‑deoxy‑7‑phosphoheptulonate synthase AroF
RA 2,744,967 A→G I260V (ATT→GTT)  nadK → NAD(+) kinase
RA 2,768,477 T→C intergenic (+97/‑514) BW25113_RS13770 → / → BW25113_RS24190 hypothetical protein/DUF905 domain‑containing protein
RA 2,787,427 T→C intergenic (+53/‑185) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA 2,795,453 C→T G249G (GGC→GGT nrdE → class 1b ribonucleoside‑diphosphate reductase subunit alpha
RA 2,834,040 A→G K386K (AAA→AAG ascF → PTS cellobiose/arbutin/salicin transporter subunit IIBC
RA 2,851,528 T→C T359T (ACT→ACC mutS → DNA mismatch repair protein MutS
RA 2,862,116 T→C intergenic (‑4/+136) nlpD ← / ← pcm murein hydrolase activator NlpD/protein‑L‑isoaspartate O‑methyltransferase
RA 2,940,916 T→C noncoding (61/77 nt) BW25113_RS14640 → tRNA‑Met
RA 2,982,682 (A)8→7 coding (20/483 nt) BW25113_RS14815 → hypothetical protein
RA 2,991,336 T→C pseudogene (852/1137 nt) BW25113_RS14890 ← glycoside hydrolase family 15 protein
RA 3,000,381 T→G V253G (GTC→GGC)  ygeW → knotted carbamoyltransferase YgeW
RA 3,014,062 A→G D55G (GAC→GGC)  ygfM → molybdopterin‑dependent oxidoreductase FAD‑binding subunit
RA 3,035,080 C→T P137S (CCT→TCT)  ygfZ → tRNA‑modifying protein YgfZ
RA 3,097,142 T→G G257G (GGT→GGG mutY → A/G‑specific adenine glycosylase
RA 3,260,970 T→C intergenic (+13/‑243) tdcR → / → BW25113_RS16190 transcriptional activator TdcR/hypothetical protein
RA 3,335,202 A→C H193P (CAC→CCC)  kdsD → arabinose‑5‑phosphate isomerase KdsD
RA 3,339,127 A→G Q351R (CAG→CGG)  rpoN → RNA polymerase factor sigma‑54
RA 3,345,818 T→C G189G (GGA→GGG arcB ← aerobic respiration two‑component sensor histidine kinase ArcB
RA 3,355,069 A→G T179A (ACC→GCC)  gltF → DUF1120 domain‑containing protein
RA 3,401,936 T→C F324S (TTC→TCC)  panF → sodium/pantothenate symporter
RA 3,450,720 T→C S329P (TCC→CCC)  gspD → type II secretion system secretin GspD
RA 3,454,146 Δ1 bp coding (971/1197 nt) gspF → type II secretion system inner membrane protein GspF
RA 3,471,902 A→G intergenic (‑46/+49) slyD ← / ← yheV peptidylprolyl isomerase/YheV family putative metal‑binding protein
RA 3,504,746 G→A G12G (GGC→GGT yhfY ← PRD domain protein
RA 3,520,900 T→C I358T (ATC→ACC)  igaA → intracellular growth attenuator protein IgaA
RA 3,527,973 T→C M419V (ATG→GTG)  envZ ← two‑component system sensor histidine kinase EnvZ
RA 3,552,176 T→C S184P (TCC→CCC)  rtcR → DNA‑binding transcriptional regulator RtcR
RA 3,596,637 A→G S323P (TCG→CCG)  ftsY ← signal recognition particle‑docking protein FtsY
RA 3,625,150 T→C R267R (AGA→AGG yhiJ ← YhiJ/YhiL family protein
RA 3,660,711 T→C D77G (GAT→GGT)  gadA ← glutamate decarboxylase
RA 3,680,898 T→C R546G (AGA→GGA)  bcsC ← cellulose synthase complex outer membrane protein BcsC
RA 3,737,651 A→G intergenic (+81/‑37) yiaL → / → yiaM YhcH/YjgK/YiaL family protein/2,3‑diketo‑L‑gulonate TRAP transporter small permease YiaM
RA 3,743,001 C→T A137V (GCG→GTG)  sgbU → L‑ribulose‑5‑phosphate 3‑epimerase
RA 3,786,139 T→C intergenic (‑228/+47) kbl ← / ← yibB glycine C‑acetyltransferase/protein YibB
RA 3,810,692 T→A D219E (GAT→GAA yicC → YicC family protein
RA 3,875,083 A→G V200A (GTG→GCG)  dnaN ← DNA polymerase III subunit beta
RA 3,879,837 C→T P468S (CCG→TCG)  yidC → membrane protein insertase YidC
RA 3,926,018 T→C L448P (CTG→CCG)  kup → low affinity potassium transporter Kup
RA 3,958,917 (T)8→7 coding (74/1266 nt) rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,964,256 T→C V255A (GTC→GCC)  wecB → UDP‑N‑acetylglucosamine 2‑epimerase (non‑hydrolyzing)
RA 3,971,768 A→G E295G (GAA→GGA)  rffT → TDP‑N‑acetylfucosamine:lipid II N‑acetylfucosaminyltransferase
RA 3,980,918 (T)5→4 coding (325/1197 nt) hemY ← protoheme IX biogenesis protein HemY
RA 3,997,421 T→C T40A (ACG→GCG)  rarD ← EamA family transporter RarD
RA 4,011,393 A→G D234G (GAC→GGC)  rmuC → DNA recombination protein RmuC
RA 4,014,046 T→C V154A (GTC→GCC)  ubiB → ubiquinone biosynthesis regulatory protein kinase UbiB
RA 4,028,476 A→G E170G (GAA→GGA)  hemG → menaquinone‑dependent protoporphyrinogen IX dehydrogenase
RA 4,039,082 T→C E294G (GAA→GGA)  yihG ← acyltransferase
RA 4,091,711 (G)5→4 coding (94/984 nt) kdgT → 2‑keto‑3‑deoxygluconate transporter
RA 4,096,618 T→C G74G (GGT→GGC fieF → CDF family cation‑efflux transporter FieF
RA 4,134,706 T→C S262P (TCC→CCC)  pflD → formate C‑acetyltransferase
RA 4,136,644 T→C T153T (ACT→ACC pflC → [formate‑C‑acetyltransferase]‑activating enzyme
RA 4,168,262 A→G intergenic (+46/‑113) nusG → / → rplK transcription termination/antitermination protein NusG/50S ribosomal protein L11
RA 4,194,831 A→C G343G (GGT→GGG purD ← phosphoribosylamine‑‑glycine ligase
RA 4,211,733 T→C E228G (GAA→GGA)  espL4 ← type III secretion system effector EspL4
RA 4,220,940 A→G E220G (GAA→GGA)  rluF → 23S rRNA pseudouridine(2604) synthase RluF
RA 4,221,148 G→A G289G (GGG→GGA rluF → 23S rRNA pseudouridine(2604) synthase RluF
RA 4,233,053 A→G F131S (TTC→TCC)  malG ← maltose ABC transporter permease MalG
RA 4,248,506 A→G R261R (CGA→CGG dinF → MATE family efflux transporter DinF
RA 4,264,658 (A)6→5 intergenic (+69/+30) ssb1 → / ← yjcB single‑stranded DNA‑binding protein SSB1/YjcB family protein
RA 4,278,646 A→C T319P (ACC→CCC)  nrfA → ammonia‑forming nitrite reductase cytochrome c552 subunit
RA 4,279,722 C→T L184F (CTT→TTT)  nrfB → cytochrome c nitrite reductase pentaheme subunit
RA 4,293,463 T→C D155G (GAC→GGC)  mdtN ← multidrug efflux transporter periplasmic adaptor subunit MdtN
RA 4,299,745 (A)6→5 coding (1053/1533 nt) alsA ← D‑allose ABC transporter ATP‑binding protein AlsA
RA 4,325,912 T→A I313F (ATT→TTT)  adiC ← arginine/agmatine antiporter
RA 4,329,995 A→G I115T (ATT→ACT)  adiA ← arginine decarboxylase
RA 4,334,519 T→C I445T (ATC→ACC)  melB → melibiose:sodium transporter MelB
RA 4,347,351 T→G T362P (ACC→CCC)  cadA ← lysine decarboxylase CadA
RA 4,379,477 T→C E234G (GAA→GGA)  asd ← archaetidylserine decarboxylase
RA 4,413,422 A→G S254G (AGC→GGC)  ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE
RA 4,423,096 A→G V249A (GTT→GCT)  qorB ← NAD(P)H:quinone oxidoreductase
RA 4,471,160 T→C D832G (GAT→GGT)  valS ← valine‑‑tRNA ligase
RA 4,474,928 T→A I281F (ATC→TTC)  pepA ← leucyl aminopeptidase
RA 4,482,153 T→C I64V (ATC→GTC)  idnT ← gnt‑II system L‑idonate transporter
RA 4,482,400 (G)6→7 intergenic (‑58/+4) idnT ← / ← idnO gnt‑II system L‑idonate transporter/gluconate 5‑dehydrogenase
RA 4,491,710 A→G intergenic (+304/+210) BW25113_RS24825 → / ← insG transposase domain‑containing protein/IS4‑like element IS4 family transposase
RA 4,504,618 T→C D626G (GAT→GGT)  fecA ← Fe(3+) dicitrate transport protein FecA
RA 4,534,634 A→G S172G (AGC→GGC)  fimC → type 1 fimbria chaperone FimC
RA 4,536,840 A→G E643G (GAA→GGA)  fimD → fimbrial usher FimD
RA 4,548,194 Δ1 bp coding (1150/1173 nt) iadA ← beta‑aspartyl‑peptidase
RA 4,590,291 T→C I168V (ATC→GTC)  dnaC ← DNA replication protein DnaC
RA 4,595,668 A→G G16G (GGA→GGG yjjZ → DUF1435 domain‑containing protein
RA 4,596,032 (G)8→9 noncoding (72/87 nt) BW25113_RS22750 ← tRNA‑Leu
RA 4,616,816 T→C V363A (GTC→GCC)  radA → DNA repair protein RadA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 235082 235120 39 2 [1] [1] 2 BW25113_RS01095 hypothetical protein
* * ÷ NZ_CP009273 295819 295819 1 2 [0] [0] 2 paoC aldehyde oxidoreductase molybdenum‑binding subunit PaoC
* * ÷ NZ_CP009273 356976 357575 600 2 [1] [1] 2 [lacA] [lacA]
* * ÷ NZ_CP009273 396692 396756 65 2 [0] [1] 2 BW25113_RS25915/iraP hypothetical protein/anti‑adapter protein IraP
* * ÷ NZ_CP009273 564255 564315 61 3 [0] [1] 2 [BW25113_RS25485] [BW25113_RS25485]
* * ÷ NZ_CP009273 576035 576049 15 2 [1] [1] 3 ylcI/nohD YlcI/YnfO family protein/DNA‑packaging protein
* * ÷ NZ_CP009273 578974 579073 100 2 [1] [1] 2 BW25113_RS26085/appY tail fiber assembly protein/DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 632127 632192 66 3 [1] [1] 4 [ybdO] [ybdO]
* * ÷ NZ_CP009273 647552 647623 72 2 [1] [0] 2 citC/dpiB [citrate (pro‑3S)‑lyase] ligase/sensor histidine kinase DpiB
* * ÷ NZ_CP009273 651722 651734 13 4 [1] [0] 2 dcuC/pagP anaerobic C4‑dicarboxylate transporter DcuC/lipid IV(A) palmitoyltransferase PagP
* * ÷ NZ_CP009273 703688 703759 72 2 [0] [1] 3 glnS/chiP glutamine‑‑tRNA ligase/chitoporin
* * ÷ NZ_CP009273 731644 731654 11 2 [1] [0] 2 BW25113_RS03640 hypothetical protein
* * ÷ NZ_CP009273 731723 731731 9 2 [0] [0] 2 BW25113_RS03640/ybfQ hypothetical protein/ISAs1 family transposase
* * ÷ NZ_CP009273 803692 803699 8 2 [0] [0] 3 bioA adenosylmethionine‑‑8‑amino‑7‑oxononanoate transaminase
* * ÷ NZ_CP009273 902995 903072 78 2 [1] [1] 2 ybjT SDR family oxidoreductase
* * ÷ NZ_CP009273 973608 973614 7 2 [1] [1] 3 mukB chromosome partition protein MukB
* * ÷ NZ_CP009273 1032569 1032591 23 2 [1] [1] 2 hyaF hydrogenase expression/formation protein
* * ÷ NZ_CP009273 1083247 1083253 7 2 [0] [1] 2 pgaC poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
* * ÷ NZ_CP009273 1098794 1098906 113 2 [1] [1] 3 csgD/csgB biofilm master transcriptional regulator CsgD/curli minor subunit CsgB
* * ÷ NZ_CP009273 1158161 1158163 3 5 [0] [1] 2 lpoB penicillin‑binding protein activator LpoB
* * ÷ NZ_CP009273 1164376 1164451 76 3 [1] [0] 2 comR/bhsA TetR family copper‑responsive transcriptional repressor ComR/multiple stress resistance protein BhsA
* * ÷ NZ_CP009273 1192481 1192533 53 2 [1] [1] 3 ymfD class I SAM‑dependent methyltransferase
* * ÷ NZ_CP009273 1193083 1193094 12 3 [0] [1] 4 BW25113_RS05920 hypothetical protein
* * ÷ NZ_CP009273 1205722 1205801 80 2 [1] [1] 3 pinE/mcrA site‑specific DNA recombinase/5‑methylcytosine‑specific endonuclease McrA
* * ÷ NZ_CP009273 1207539 1207585 47 2 [1] [1] 2 iraM/BW25113_RS25985 anti‑adapter protein IraM/protein YmgK
* * ÷ NZ_CP009273 1214433 1214442 10 2 [0] [0] 2 [BW25113_RS06070] [BW25113_RS06070]
* * ÷ NZ_CP009273 1261670 1261701 32 2 [1] [1] 3 prmC peptide chain release factor N(5)‑glutamine methyltransferase
* * ÷ NZ_CP009273 1287849 1287907 59 3 [1] [1] 2 galU/hns UTP‑‑glucose‑1‑phosphate uridylyltransferase GalU/DNA‑binding transcriptional regulator H‑NS
* * ÷ NZ_CP009273 1314770 1314858 89 2 [1] [1] 2 trpD bifunctional anthranilate synthase glutamate amidotransferase component TrpG/anthranilate phosphoribosyltransferase TrpD
* * ÷ NZ_CP009273 1329154 1329164 11 5 [0] [1] 2 cysB/ymiA HTH‑type transcriptional regulator CysB/YmiA family putative membrane protein
* * ÷ NZ_CP009273 1366072 1366097 26 4 [1] [1] 2 ycjM glucosylglycerate phosphorylase
* * ÷ NZ_CP009273 1419270 1419298 29 2 [1] [1] 2 trkG Trk system potassium transporter TrkG
* * ÷ NZ_CP009273 1459415 1459480 66 3 [1] [1] 4 [BW25113_RS25580] [BW25113_RS25580]
* * ÷ NZ_CP009273 1748980 1749083 104 2 [1] [1] 2 ydhY/fumD ferredoxin‑like protein/fumarate hydratase FumD
* * ÷ NZ_CP009273 1772965 1773041 77 2 [1] [1] 2 ydiP AraC family transcriptional regulator
* * ÷ NZ_CP009273 2050824 2050848 25 2 [1] [1] 2 yeeN YebC/PmpR family DNA‑binding transcriptional regulator
* * ÷ NZ_CP009273 2051046 2051081 36 2 [0] [1] 2 [yeeN] [yeeN]
* * ÷ NZ_CP009273 2078985 2079002 18 2 [0] [1] 3 yeeE thiosulfate utilization transporter TsuA/YeeE
* * ÷ NZ_CP009273 2157889 2157901 13 2 [1] [1] 2 baeR two‑component system response regulator BaeR
* * ÷ NZ_CP009273 2211840 2211895 56 2 [1] [1] 3 yehY glycine betaine ABC transporter permease YehY
* * ÷ NZ_CP009273 2300310 2300381 72 2 [1] [1] 4 mqo/yojI malate dehydrogenase (quinone)/microcin J25 efflux ABC transporter YojI
* * ÷ NZ_CP009273 2463170 2463217 48 2 [1] [1] 2 BW25113_RS12270 hypothetical protein
* * ÷ NZ_CP009273 2898900 2898949 50 2 [1] [1] 2 [BW25113_RS14445] [BW25113_RS14445]
* * ÷ NZ_CP009273 3165523 3165533 11 4 [1] [1] 4 ygiZ DUF2645 family protein
* * ÷ NZ_CP009273 3262656 3262657 2 2 [0] [0] 2 yhaC YhaC family protein
* * ÷ NZ_CP009273 3576017 3576032 16 2 [1] [1] 2 hcp Hcp1 family type VI secretion system effector
* * ÷ NZ_CP009273 3647059 3647117 59 4 [1] [1] 2 BW25113_RS18165/slp hypothetical protein/outer membrane lipoprotein Slp
* * ÷ NZ_CP009273 3763096 3763127 32 2 [0] [0] 2 BW25113_RS26240 Imm57 family immunity protein
* * ÷ NZ_CP009273 3763181 3763183 3 2 [0] [0] 2 BW25113_RS26240/yibH Imm57 family immunity protein/HlyD family secretion protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =NA (NA)21 (1.180) 16/166 0.2 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 = 1391494NA (NA)7 (0.400) 3/164 1.5 78.0% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 2 (0.110)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)37 (2.080) 20/166 0.1 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 4532499 =3 (0.170)6 (0.380) 5/148 1.0 43.4% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 13 (0.820)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA