breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 310 (T)9→8 intergenic (+55/‑27) thrL → / → thrA thr operon leader peptide/fused aspartate kinase/homoserine dehydrogenase 1
RA 3,496 A→G E232E (GAA→GAG thrB → homoserine kinase
RA 15,231 A→G H355R (CAC→CGC)  dnaJ → chaperone protein DnaJ
RA 16,753 A→G *51Q (TAA→CAA) 
*70Q (TAA→CAA) 
hokC ←
mokC ←
protein HokC
regulatory protein MokC
RA 29,918 A→G S90G (AGC→GGC)  carA → carbamoyl phosphate synthetase subunit alpha
RA 34,120 (T)9→8 intergenic (+82/‑180) carB → / → caiF carbamoyl phosphate synthetase subunit beta/DNA‑binding transcriptional activator CaiF
RA 34,807 T→C T189A (ACG→GCG)  caiE ← putative transferase CaiE
RA 39,357 T→C N344D (AAC→GAC)  caiA ← crotonobetainyl‑CoA reductase
RA 66,079 T→C I158V (ATC→GTC)  araD ← L‑ribulose‑5‑phosphate 4‑epimerase AraD
RA 83,579 (A)7→6 intergenic (‑50/+43) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 84,673 T→C G102G (GGT→GGC leuO → DNA‑binding transcriptional dual regulator LeuO
RA 85,841 T→C V71A (GTG→GCG)  ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 99,144 A→G T248A (ACC→GCC)  ftsW → putative lipid II flippase FtsW
RA 106,009 T→C R235R (CGT→CGC ftsZ → cell division protein FtsZ
RA 124,766 A→G T584A (ACC→GCC)  aceE → pyruvate dehydrogenase E1 component
RA 134,527 A→G H47R (CAC→CGC)  yacL → UPF0231 family protein YacL
RA 135,481 A→G G34G (GGT→GGC speD ← S‑adenosylmethionine decarboxylase proenzyme
RA 135,923 C→T C181Y (TGC→TAC)  speE ← spermidine synthase
RA 140,467 C→T E253E (GAG→GAA gcd ← quinoprotein glucose dehydrogenase
RA 142,598 A→G F25L (TTT→CTT)  can ← carbonic anhydrase 2
RA 166,504 C→T T592I (ACC→ATC)  mrcB → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
RA 169,743 A→G intergenic (+16/‑35) fhuA → / → fhuC ferrichrome outer membrane transporter/phage receptor/iron(III) hydroxamate ABC transporter ATP binding subunit
RA 177,523 T→C V34V (GTA→GTG yadS ← conserved inner membrane protein YadS
RA 203,098 A→G D180G (GAC→GGC)  lpxA → UDP‑N‑acetylglucosamine acyltransferase
RA 214,585 G→A A99T (GCC→ACC)  yaeQ → uncharacterized protein YaeQ
RA 214,689 T→C Y133Y (TAT→TAC yaeQ → uncharacterized protein YaeQ
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 262,313 C→T P271S (CCC→TCC)  proA → glutamate‑5‑semialdehyde dehydrogenase
RA 272,712 (G)5→6 intergenic (+58/‑135) eyeA → / → ykfC CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC
RA 277,660 T→G intergenic (+13/+96) mmuM → / ← afuC homocysteine S‑methyltransferase/CP4‑6 prophage; ABC transporter ATP‑binding protein AfuC
RA 278,741 A→G V21A (GTA→GCA)  afuC ← CP4‑6 prophage; ABC transporter ATP‑binding protein AfuC
RA 293,799 T→C G40G (GGA→GGG yagL ← CP4‑6 prophage; resolvase‑like catalytic domain‑containing protein YagL
RA 301,670 A→G V73A (GTA→GCA)  paoB ← aldehyde dehydrogenase, FAD‑binding subunit
RA 309,710 C→T R106H (CGC→CAC)  ecpB ← putative fimbrial chaperone EcpB
RA 310,333 G→A G113G (GGC→GGT ecpA ← common pilus major subunit
RA 319,208 C→T G265D (GGT→GAT)  rclA ← putative pyridine nucleotide‑disulfide oxidoreductase RclA
RA 321,564 (G)7→8 intergenic (+483/‑44) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 337,142 A→G N122S (AAC→AGC)  yahF → putative acyl‑CoA synthetase YahF
RA 343,357 T→C C158C (TGT→TGC yahK → aldehyde reductase, NADPH‑dependent
RA 350,390 C→T H127Y (CAT→TAT)  prpC → 2‑methylcitrate synthase
RA 363,780 C→T A842A (GCG→GCA lacZ ← beta‑galactosidase
RA 367,852 (GC)6→5 coding (568‑569/834 nt) mhpR ← DNA‑binding transcriptional activator MhpR
RA 370,220 C→T S537L (TCG→TTG)  mhpA → putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
RA 372,855 C→T A247A (GCC→GCT mhpD → 2‑hydroxypentadienoate hydratase
RA 378,772 A→G V267A (GTC→GCC)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA 391,703 (G)6→7 pseudogene (1453/1458 nt) yaiT → putative autotransporter YaiT
RA 393,177 A→G pseudogene (208/1449 nt) yaiT → putative autotransporter YaiT
RA 401,893 T→C G49G (GGT→GGC phoA → alkaline phosphatase
RA 410,100 T→C intergenic (‑81/‑44) rdgC ← / → mak nucleoid‑associated protein RdgC/manno(fructo)kinase
RA 415,162 T→C T198A (ACA→GCA)  sbcC ← ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
RA 420,893 A→G T435A (ACC→GCC)  brnQ → branched chain amino acid transporter BrnQ
RA 433,447 A→G D149G (GAC→GGC)  nrdR → NrdR transcriptional repressor
RA 473,912 C→T A316V (GCG→GTG)  amtB → ammonia/ammonium transporter
RA 476,031 A→G V114A (GTA→GCA)  atl ← DNA base‑flipping protein
RA 494,736 A→G H111R (CAT→CGT)  recR → DNA repair protein RecR
RA 523,668 T→C S136S (AGT→AGC rhsD → protein RhsD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 531,988 A→G S78S (AGT→AGC allS ← DNA‑binding transcriptional activator AllS
RA 546,454 (A)7→8 intergenic (‑91/‑226) allD ← / → fdrA ureidoglycolate dehydrogenase/putative acyl‑CoA synthetase FdrA
RA 553,219 (T)6→5 coding (722/723 nt) lpxH ← UDP‑2,3‑diacylglucosamine diphosphatase
RA 562,803 A→G R156R (AGA→AGG sfmH → putative fimbrial adhesin protein SfmH
RA 568,195 C→T pseudogene (161/213 nt) renD → DLP12 prophage; protein RenD
RA 568,239 A→G pseudogene (205/213 nt) renD → DLP12 prophage; protein RenD
RA 585,935 A→G intergenic (‑302/+212) ompT ← / ← envY outer membrane protease VII (outer membrane protein 3b)/DNA‑binding transcriptional activator EnvY
RA 593,357 T→C T472A (ACA→GCA)  cusS ← sensory histidine kinase CusS
RA 617,030 A→G L958L (TTA→TTG entF → apo‑serine activating enzyme
RA 630,875 T→C F328L (TTC→CTC)  cstA → carbon starvation protein A
RA 660,601 A→G L260L (TTA→CTA)  ybeF ← putative LysR‑type DNA‑binding transcriptional regulator YbeF
MC JC 676,374 Δ11,477 bp IS5‑mediated [ybeQ]gltI [ybeQ], ybeR, djlB, ybeT, ybeU, djlC, hscC, rihA, gltL, gltK, gltJ, gltI
RA 709,068 G→A G245G (GGG→GGA chiP → chitobiose outer membrane channel
RA 717,696 A→G S190S (AGT→AGC potE ← putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
RA 764,243 A→G T22A (ACT→GCT)  sucD → succinyl‑CoA synthetase subunit alpha
RA 766,367 (C)6→7 coding (384/1977 nt) mngA → 2‑O‑alpha‑mannosyl‑D‑glycerate specific PTS enzyme II
RA 781,812 T→C intergenic (+160/‑273) lysQ → / → nadA tRNA‑Lys/quinolinate synthase
RA 874,140 T→C S388P (TCT→CCT)  pdeI → putative c‑di‑GMP phosphodiesterase PdeI
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 918,591 C→T G177D (GGT→GAT)  ybjX ← DUF535 domain‑containing protein YbjX
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 938,685 T→C V231A (GTC→GCC)  rarA → recombination factor
RA 945,893 T→C V8A (GTC→GCC)  ycaD → putative transporter YcaD
RA 952,608 A→G V316A (GTT→GCT)  pflB ← pyruvate formate‑lyase
RA 986,740 A→G G81G (GGT→GGC ompF ← outer membrane porin F
RA 991,358 A→G D246G (GAC→GGC)  pepN → aminopeptidase N
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,017,932 A→G V123A (GTA→GCA)  ycbZ ← putative ATP‑dependent protease YcbZ
RA 1,023,368 A→G R172R (CGT→CGC yccS ← putative transporter YccS
RA 1,045,590 C→T A72T (GCT→ACT)  gfcE ← putative exopolysaccharide export lipoprotein GfcE
RA 1,047,955 A→G V246A (GTT→GCT)  gfcC ← capsule biosynthesis GfcC family protein
RA 1,052,330 A→G Y345H (TAC→CAC)  yccM ← putative 4Fe‑4S membrane protein
RA 1,059,367 A→G T38A (ACT→GCT)  torA → trimethylamine N‑oxide reductase 1
RA 1,103,745 (T)7→8 intergenic (‑549/‑206) csgD ← / → csgB DNA‑binding transcriptional dual regulator CsgD/curlin, minor subunit
RA 1,110,661 A→G T443A (ACC→GCC)  opgG → osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
RA 1,120,223 T→C I122V (ATT→GTT)  solA ← N‑methyl‑L‑tryptophan oxidase
RA 1,132,417 T→C G188G (GGT→GGC flgD → flagellar biosynthesis, initiation of hook assembly
RA 1,133,535 A→G N321S (AAC→AGC)  flgE → flagellar hook protein FlgE
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,168,020 G→A G141D (GGT→GAT)  ycfJ → PF05433 family protein YcfJ
RA 1,190,998 A→C V330G (GTT→GGT)  purB ← adenylosuccinate lyase
RA 1,196,817 A→G intergenic (+444/+50) icd → / ← ymfD isocitrate dehydrogenase/e14 prophage; putative SAM‑dependent methyltransferase
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,212,933 T→C T59A (ACC→GCC)  ycgX ← uncharacterized protein YcgX
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,221,752 T→C pseudogene (2152/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,235,586 C→T H217Y (CAT→TAT)  fadR → DNA‑binding transcriptional dual regulator FadR
RA 1,240,425 A→G K56E (AAA→GAA) 
F549F (TTT→TTC
ymgM →
cvrA ←
protein YmgM
putative K(+):H(+) antiporter
RA 1,245,862 (C)6→5 coding (1515/1698 nt) treA ← periplasmic trehalase
RA 1,253,367 Δ1 bp coding (2586/2868 nt) ycgV ← putative autotransporter adhesin YcgV
RA 1,267,217 G→T M98I (ATG→ATT ychQ → SirB family protein YchQ
RA 1,281,830 T→C L656P (CTG→CCG)  narG → nitrate reductase A subunit alpha
RA 1,287,326 A→G noncoding (3/85 nt) tyrV ← tRNA‑Tyr
RA 1,287,514 T→C intergenic (‑186/+24) tyrV ← / ← tyrT tRNA‑Tyr/tRNA‑Tyr
RA 1,299,211 G→C G205A (GGT→GCT)  ychE → putative inner membrane protein
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,302,986 G→A A30T (GCG→ACG)  oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,303,712 T→C Y272H (TAT→CAT)  oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,310,097 T→A D58V (GAT→GTT)  kch ← K(+) channel Kch
RA 1,311,303 A→G E72G (GAA→GGA)  tonB → Ton complex subunit TonB
RA 1,361,590 T→C H157H (CAT→CAC puuD → gamma‑glutamyl‑gamma‑aminobutyrate hydrolase
RA 1,368,168 T→C R30R (CGT→CGC pspA → phage shock protein A
RA 1,369,805 G→A G39G (GGG→GGA pspE → thiosulfate sulfurtransferase PspE
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
RA 1,373,620 C→T N133N (AAC→AAT ycjO → putative ABC transporter membrane subunit YcjO
RA 1,376,074 A→G N14S (AAC→AGC)  ycjR → putative ketohexose‑3‑epimerase YcjR
RA 1,381,779 (G)5→6 pseudogene (959/969 nt) ycjV → putative ABC transporter ATP‑binding protein YcjV
RA 1,390,897 A→G W13R (TGG→CGG)  ymjD ← uncharacterized protein YmjD
RA 1,399,985 (C)7→9 coding (252/516 nt) ogt ← methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
RA 1,422,408 T→C S142S (AGT→AGC ydaV → Rac prophage; putative ATP‑binding protein YdaV
RA 1,429,013 C→T intergenic (+29/‑36) lomR → / → stfR Rac prophage; protein LomR_1/Rac prophage; putative membrane protein
RA 1,433,681 A→G intergenic (‑7/+310) pinR ← / ← ynaE Rac prophage; putative site‑specific recombinase/Rac prophage; uncharacterized protein YnaE
RA 1,438,800 T→C D662G (GAC→GGC)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,447,975 (G)5→4 coding (457/1500 nt) feaB → phenylacetaldehyde dehydrogenase
RA 1,451,427 A→G intergenic (‑78/+170) tynA ← / ← paaZ copper‑containing amine oxidase/oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,478,201 T→C V194A (GTG→GCG)  ynbA → putative inner membrane protein
RA 1,481,238 T→C S110S (AGT→AGC ynbD → putative phosphatase
RA 1,495,757 +C coding (470/1344 nt) ydcJ → DUF1338 domain‑containing protein YdcJ
RA 1,529,350 Δ1 bp pseudogene (1461/2049 nt) rhsE → RhsE protein in rhs element
RA 1,533,261 A→G K70K (AAA→AAG pptA → putative 4‑oxalocrotonate tautomerase (4‑OT)
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,542,818 C→T G415S (GGC→AGC)  narU ← nitrate/nitrite transporter NarU
RA 1,548,895 A→G S499G (AGC→GGC)  fdnG → formate dehydrogenase N subunit alpha
RA 1,549,816 A→G T806A (ACG→GCG)  fdnG → formate dehydrogenase N subunit alpha
RA 1,555,706 (G)6→7 intergenic (‑37/+120) maeA ← / ← sra malate dehydrogenase, NAD‑requiring/30S ribosomal subunit protein S22
RA 1,559,226 C→A A229S (GCC→TCC)  ddpC ← putative D,D‑dipeptide ABC transporter membrane subunit DdpC
RA 1,577,704 C→T V547I (GTC→ATC)  yddA ← ABC transporter family protein YddA
RA 1,580,434 (G)5→6 coding (357/1158 nt) ydeM ← putative anaerobic sulfatase maturation enzyme YdeM
RA 1,584,679 C→T G603D (GGT→GAT)  ydeP ← putative oxidoreductase YdeP
RA 1,593,020 G→A pseudogene (5067/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,594,132 (C)8→9 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
JC 1,594,256 (C)8→11 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,608,512 G→A R171C (CGC→TGC)  yneE ← conserved inner membrane protein YneE
RA 1,612,026 T→C Q100Q (CAA→CAG yneG ← conserved protein YneG
RA 1,629,024 T→C F225L (TTT→CTT)  ydfH → DNA‑binding transcriptional repressor YdfH
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,702,016 T→C Y354H (TAT→CAT)  malY → negative regulator of MalT activity/cystathionine beta‑lyase
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,718,194 T→C I68V (ATT→GTT)  mliC ← inhibitor of c‑type lysozyme, putative lipoprotein
RA 1,728,495 G→A G50D (GGC→GAC)  rnt → RNase T
RA 1,755,537 T→C intergenic (‑396/‑161) fumD ← / → pykF fumarase D/pyruvate kinase I
RA 1,757,564 G→A V48V (GTG→GTA lpp → murein lipoprotein
RA 1,783,511 G→A A179T (GCT→ACT)  fadK → short chain acyl‑CoA synthetase
RA 1,814,387 G→A G174D (GGC→GAC)  katE → catalase II
RA 1,836,865 C→A L265M (CTG→ATG)  ynjB → putative ABC transporter periplasmic binding protein YnjB
RA 1,842,809 (T)5→6 coding (439/1344 nt) gdhA → glutamate dehydrogenase
RA 1,854,423 T→C E144E (GAA→GAG ydjF ← putative DNA‑binding transcriptional regulator YdjF
RA 1,855,920 A→G L18L (TTG→CTG)  ydjG ← NADH‑dependent methylglyoxal reductase
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,876,011 T→C G113G (GGT→GGC nimT → 2‑nitroimidazole exporter
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,880,277 T→C E161E (GAA→GAG leuE ← leucine exporter
RA 1,886,332 C→T P163L (CCG→CTG)  yeaX → carnitine monooxygenase subunit YeaX
RA 1,891,040 G→A G76G (GGC→GGT tsaB ← N(6)‑L‑threonylcarbamoyladenine synthase, TsaB subunit
RA 1,894,728 A→G F2L (TTT→CTT)  yoaH ← UFP0181 family protein YoaH
RA 1,896,063 T→C F420S (TTC→TCC)  pabB → aminodeoxychorismate synthase subunit 1
RA 1,900,391 T→G T399P (ACC→CCC)  yoaE ← putative inner membrane protein
RA 1,900,530 T→C E352E (GAA→GAG yoaE ← putative inner membrane protein
RA 1,902,757 G→A G237D (GGC→GAC)  manX → mannose‑specific PTS enzyme IIAB component
RA 1,906,205 T→C V173A (GTG→GCG)  mntP → Mn(2(+)) exporter
RA 1,924,683 A→G L96P (CTG→CCG)  yobA ← CopC domain‑containing protein
RA 1,976,103 G→A S51S (AGC→AGT motB ← motility protein B
RA 1,977,784 T→C T21A (ACC→GCC)  flhC ← DNA‑binding transcriptional dual regulator FlhC
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,994,589 (T)6→7 coding (118/1833 nt) uvrC ← excision nuclease subunit C
RA 2,020,172 T→C V29A (GTT→GCT)  fliM → flagellar motor switch protein FliM
RA 2,021,360 T→C G91G (GGT→GGC fliN → flagellar motor switch protein FliN
RA 2,024,373 T→C L136L (TTA→CTA)  rcsA → DNA‑binding transcriptional activator RcsA
RA 2,032,339 A→G intergenic (‑22/+45) dcm ← / ← yedJ DNA‑cytosine methyltransferase/putative HD superfamily phosphohydrolase YedJ
RA 2,062,074 G→A intergenic (‑141/‑186) nac ← / → asnV DNA‑binding transcriptional dual regulator Nac/tRNA‑Asn
RA 2,083,678 T→C V308A (GTG→GCG)  sbcB → exodeoxyribonuclease I
RA 2,084,598 T→C M310V (ATG→GTG)  yeeE ← inner membrane protein YeeE
RA 2,112,050 T→C T338A (ACA→GCA)  rfbB ← dTDP‑glucose 4,6‑dehydratase 1
RA 2,128,358 T→C E177E (GAA→GAG wcaF ← putative acyl transferase
RA 2,132,568 (C)5→6 coding (830/840 nt) wcaA ← putative colanic acid biosynthesis glycosyl transferase
RA 2,155,279 (C)5→6 coding (17/3123 nt) mdtB → multidrug efflux pump RND permease subunit MdtB
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,195,930 T→C V544A (GTG→GCG)  metG → methionine‑‑tRNA ligase
RA 2,260,182 A→G V410A (GTA→GCA)  fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,265,637 A→G D63G (GAC→GGC)  yeiP → elongation factor P family protein
RA 2,268,347 T→C G173G (GGT→GGC yeiR → zinc‑binding GTPase YeiR
RA 2,271,035 (A)7→8 coding (310/1557 nt) pdeN → putative c‑di‑GMP phosphodiesterase PdeN
RA 2,278,701 +C coding (1060/1191 nt) bcr ← multidrug efflux pump Bcr
RA 2,288,561 (C)7→6 pseudogene (354/2525 nt) yejO ← adhesin‑like autotransporter YejO
RA 2,309,422 A→G D328D (GAT→GAC ada ← DNA‑binding transcriptional dual regulator/DNA repair protein Ada
RA 2,312,141 A→G G203G (GGT→GGC ompC ← outer membrane porin C
RA 2,325,859 A→G G368G (GGA→GGG atoE → short chain fatty acid transporter
RA 2,326,925 (C)6→7 coding (817/1185 nt) atoB → acetyl‑CoA acetyltransferase
RA 2,402,849 A→G F333L (TTC→CTC)  nuoC ← NADH:quinone oxidoreductase subunit CD
RA 2,405,417 (C)6→7 intergenic (‑345/+286) nuoA ← / ← lrhA NADH:quinone oxidoreductase subunit A/DNA‑binding transcriptional dual regulator LrhA
RA 2,407,204 G→A intergenic (‑563/‑357) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
RA 2,408,429 T→C L290P (CTA→CCA)  alaA → glutamate‑‑pyruvate aminotransferase AlaA
RA 2,418,281 G→A A401T (GCC→ACC)  yfcC → putative transporter YfcC
RA 2,425,237 T→C D227G (GAC→GGC)  hisQ ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ
RA 2,437,859 T→C intergenic (‑8/‑91) pdxB ← / → flk erythronate‑4‑phosphate dehydrogenase/putative flagella assembly protein
RA 2,440,162 T→C intergenic (‑42/+223) yfcJ ← / ← fabB putative transporter YfcJ/beta‑ketoacyl‑[acyl carrier protein] synthase I
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,462,889 (C)9→10 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,500,707 A→G T109A (ACC→GCC)  ypdB → DNA‑binding transcriptional activator YpdB
RA 2,508,092 T→C T51A (ACG→GCG)  fryB ← putative PTS enzyme IIB component FryB
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,575,328 T→C T44A (ACG→GCG)  eutP ← putative ethanolamine utilization protein EutP with nucleoside triphosphate hydrolase domain
RA 2,585,419 T→C H36R (CAC→CGC)  aegA ← putative oxidoreductase Fe‑S subunit AegA
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,605,744 T→C F312L (TTC→CTC)  hyfD → hydrogenase 4 component D
RA 2,644,623 T→C N81S (AAC→AGC)  ndk ← nucleoside diphosphate kinase
RA 2,658,436 C→T G167D (GGC→GAC)  hscA ← iron‑sulfur cluster biosynthesis chaperone HscA
RA 2,669,362 T→C S111P (TCT→CCT)  hcaE → putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
RA 2,700,461 (T)5→4 intergenic (‑264/‑59) shoB ← / → ohsC toxic peptide ShoB/small regulatory RNA OhsC
RA 2,710,853 T→C V145A (GTG→GCG)  nadB → L‑aspartate oxidase
RA 2,723,976 A→G H417R (CAC→CGC)  pssA → phosphatidylserine synthase
RA 2,741,920 A→G T16A (ACG→GCG)  yfiR → DUF4154 domain‑containing protein YfiR
RA 2,745,169 T→C G57G (GGA→GGG trmD ← tRNA m(1)G37 methyltransferase
RA 2,758,296 T→C H102R (CAC→CGC)  yfjH ← CP4‑57 prophage; protein YfjH
RA 2,767,445 (C)7→8 intergenic (+90/‑265) rnlB → / → yfjP CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP
RA 2,804,405 T→C S302P (TCC→CCC)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,808,863 G→A pseudogene (548/1178 nt) ygaY → putative transporter YgaY, N‑terminal fragment
RA 2,809,853 T→C A79A (GCT→GCC ygaZ → L‑valine exporter subunit YgaZ
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,816,592 (A)8→7 coding (349/429 nt) yqaA ← DedA family protein YqaA
RA 2,829,702 T→C V219A (GTT→GCT)  srlR → DNA‑binding transcriptional repressor SrlR
RA 2,834,893 A→C T328P (ACC→CCC)  norW → NADH:flavorubredoxin reductase
RA 2,835,501 T→C D642G (GAC→GGC)  hypF ← carbamoyltransferase HypF
RA 2,850,651 A→G H2R (CAC→CGC)  hypA → hydrogenase 3 nickel incorporation protein HypA
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,862,697 A→G G121G (GGA→GGG ygbK → putative 3‑oxo‑tetronate kinase YgbK
RA 2,867,559 C→T intergenic (‑8/+55) rpoS ← / ← nlpD RNA polymerase, sigma S (sigma 38) factor/murein hydrolase activator NlpD
RA 2,885,998 A→G S408P (TCA→CCA)  cas3 ← CRISPR‑associated endonuclease/helicase Cas3
RA 2,893,121 T→C G155G (GGT→GGC ygcN → putative oxidoreductase with FAD/NAD(P)‑binding domain
RA 2,904,097 +G intergenic (+723/+650) ygcE → / ← queE putative sugar kinase YgcE/putative 7‑carboxy‑7‑deazaguanine synthase QueE
RA 2,924,416 T→C noncoding (109/369 nt) csrB ← small regulatory RNA CsrB
RA 2,943,252 T→C intergenic (‑107/‑85) ygdI ← / → csdA DUF903 domain‑containing lipoprotein YgdI/cysteine sulfinate desulfinase
RA 2,944,020 T→C G228G (GGT→GGC csdA → cysteine sulfinate desulfinase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,954,152 T→C T618A (ACG→GCG)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,958,339 T→C A182A (GCA→GCG ptrA ← protease 3
RA 2,963,665 C→T A18T (GCG→ACG)  ppdB ← conserved protein PpdB
RA 2,982,381 Δ1 bp intergenic (‑199/+116) araE ← / ← kduD arabinose:H(+) symporter/putative 2‑keto‑3‑deoxy‑D‑gluconate dehydrogenase
RA 2,998,712 (C)5→4 coding (117/795 nt) ygeQ ← protein YgeQ
RA 3,004,064 T→C I575V (ATC→GTC)  ygeV ← putative sigma(54)‑dependent transcriptional regulator YgeV
RA 3,026,626 T→C R287R (CGT→CGC guaD → guanine deaminase
RA 3,028,327 T→C F403L (TTC→CTC)  ghxQ → guanine/hypoxanthine transporter GhxQ
RA 3,033,402 T→C V113A (GTG→GCG)  idi → isopentenyl‑diphosphate Delta‑isomerase
RA 3,044,431 T→C V257A (GTG→GCG)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,047,030 A→G V671A (GTG→GCG)  gcvP ← glycine decarboxylase
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,076,876 T→C Y194C (TAC→TGC)  yggP ← putative zinc‑binding dehydrogenase YggP
RA 3,084,761 T→C Y384C (TAC→TGC)  speA ← arginine decarboxylase, biosynthetic
RA 3,108,115 T→C K347K (AAA→AAG speC ← ornithine decarboxylase, biosynthetic
RA 3,111,500 Δ1 bp pseudogene (489/861 nt) yghE ← putative type II secretion system L‑type protein
RA 3,116,901 C→A A738S (GCG→TCG)  yghJ ← putative lipoprotein YghJ
RA 3,133,301 +G coding (657/714 nt) yghS ← putative ATP‑binding protein YghS
RA 3,177,509 (C)6→7 coding (402/630 nt) nudF ← ADP‑sugar pyrophosphatase
RA 3,180,525 T→C D35D (GAT→GAC ygiC → putative enzyme with ATPase activity
RA 3,196,428 A→G V109A (GTA→GCA)  rfaE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
RA 3,212,597 T→C G497G (GGT→GGC dnaG → DNA primase
RA 3,216,313 T→C Q60R (CAA→CGA)  nfeF ← NADPH‑dependent ferric chelate reductase
RA 3,226,934 T→C I234T (ATC→ACC)  ygjI → putative transporter YgjI
RA 3,236,318 T→C V120A (GTG→GCG)  ygjP → putative metal‑dependent hydrolase
RA 3,272,344 Δ1 bp coding (408/885 nt) garR ← tartronate semialdehyde reductase
RA 3,278,656 T→C T4A (ACC→GCC)  agaR ← DNA‑binding transcriptional repressor AgaR
RA 3,304,937 A→G V294A (GTA→GCA)  mtr ← tryptophan:H(+) symporter Mtr
RA 3,328,842 T→C intergenic (‑127/‑121) greA ← / → dacB transcription elongation factor GreA/peptidoglycan DD‑endopeptidase DacB
RA 3,344,042 T→C G33G (GGT→GGC lptB → lipopolysaccharide transport system ATP binding protein
RA 3,353,609 T→C T148A (ACC→GCC)  yhcC ← radical SAM family oxidoreductase YhcC
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,379,266 (C)7→8 coding (732/1128 nt) zapE ← cell division factor ZapE with ATPase activity
RA 3,381,378 T→C G212G (GGT→GGC degQ → periplasmic serine endoprotease
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,406,735 T→C G273G (GGT→GGC accC → biotin carboxylase
RA 3,414,734 T→C F291L (TTT→CTT)  acrE → multidrug efflux pump membrane fusion lipoprotein AcrE
RA 3,422,481 G→A E16K (GAA→AAA)  yhdZ → putative ABC transporter ATP‑binding subunit YhdZ
RA 3,433,146 T→C T139A (ACA→GCA)  smf ← protein Smf
RA 3,438,922 A→T I70N (ATC→AAC)  zntR ← DNA‑binding transcriptional activator ZntR
RA 3,459,986 G→A S57N (AGC→AAC)  gspF → Type II secretion system protein GspF
RA 3,475,341 T→C V126V (GTA→GTG tusD ← sulfurtransferase complex subunit TusD
RA 3,494,514 T→C G168G (GGT→GGC nirB → nitrite reductase catalytic subunit NirB
RA 3,494,795 (G)5→6 coding (785/2544 nt) nirB → nitrite reductase catalytic subunit NirB
RA 3,499,158 T→C V444A (GTT→GCT)  cysG → siroheme synthase
RA 3,505,168 A→G F285L (TTT→CTT)  yhfS ← putative aminotransferase YhfS
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,599,441 T→C D73G (GAC→GGC)  livJ ← branched chain amino acid/phenylalanine ABC transporter periplasmic binding protein
RA 3,632,997 (T)5→6 pseudogene (1462/1607 nt) yhiL ← putative uncharacterized protein YhiL
RA 3,693,321 T→C Y639C (TAC→TGC)  bcsA ← cellulose synthase catalytic subunit
RA 3,712,670 T→C S89G (AGC→GGC)  yhjY ← conserved protein YhjY
RA 3,714,397 C→T E666E (GAG→GAA bisC ← biotin sulfoxide reductase
RA 3,717,565 A→G T86A (ACC→GCC)  ghrB → glyoxylate reductase
RA 3,723,631 T→C E256G (GAG→GGG)  glyS ← glycine‑‑tRNA ligase subunit beta
RA 3,728,118 (C)8→9 coding (1254/1455 nt) xylB ← xylulokinase
RA 3,762,200 A→G A6A (GCA→GCG rhsA → rhs element protein RhsA
RA 3,762,212 C→T G10G (GGC→GGT rhsA → rhs element protein RhsA
RA 3,766,021 G→A W1280* (TGG→TAG)  rhsA → rhs element protein RhsA
RA 3,781,031 (C)5→4 intergenic (+14/‑184) lldD → / → trmL L‑lactate dehydrogenase/tRNA (cytidine/uridine‑2'‑O)‑ribose methyltransferase
RA 3,790,817 T→C I177V (ATT→GTT)  tdh ← threonine dehydrogenase
RA 3,810,369 A→G F262L (TTT→CTT)  mutM ← DNA‑formamidopyrimidine glycosylase
RA 3,811,671 A→G S2P (TCC→CCC)  rpmB ← 50S ribosomal subunit protein L28
RA 3,815,808 (A)8→7 intergenic (‑40/+55) pyrE ← / ← rph orotate phosphoribosyltransferase/truncated RNase PH
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,835,367 A→G V188A (GTC→GCC)  yicJ ← putative xyloside transporter YicJ
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,880,025 T→C D32G (GAC→GGC)  gyrB ← DNA gyrase subunit B
RA 3,880,855 T→C T123A (ACT→GCT)  recF ← DNA repair protein RecF
RA 3,905,639 (A)9→8 intergenic (‑42/+92) bglF ← / ← bglG beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase/transcriptional antiterminator BglG
RA 3,907,703 T→C T222A (ACG→GCG)  pstB ← phosphate ABC transporter ATP binding subunit
RA 3,910,466 T→C intergenic (‑68/+19) pstC ← / ← pstS phosphate ABC transporter membrane subunit PstC/phosphate ABC transporter periplasmic binding protein
RA 3,931,183 (G)7→8 intergenic (‑90/‑133) ravA ← / → kup regulatory ATPase RavA/K(+):H(+) symporter Kup
RA 3,959,045 T→C V359A (GTA→GCA)  ilvC → ketol‑acid reductoisomerase (NADP(+))
RA 3,970,974 A→G N281S (AAC→AGC)  wecB → UDP‑N‑acetylglucosamine 2‑epimerase
RA 3,982,684 (C)8→9 noncoding (79/87 nt) leuT → tRNA‑Leu
RA 4,060,843 (G)6→7 coding (397/711 nt) yihL → putative transcriptional regulator YihL
RA 4,063,466 A→G K407R (AAG→AGG)  yihN → putative transporter YihN
RA 4,070,450 T→C intergenic (‑49/+65) yihR ← / ← yihS putative aldose 1‑epimerase YihR/sulfoquinovose isomerase
RA 4,097,658 T→G intergenic (‑210/‑78) rhaB ← / → rhaS rhamnulokinase/DNA‑binding transcriptional activator RhaS
RA 4,103,728 A→G G416G (GGT→GGC cpxA ← sensory histidine kinase CpxA
RA 4,132,627 (T)6→7 coding (12/891 nt) metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,144,838 A→G T282A (ACA→GCA)  pflD → putative formate acetyltransferase 2
RA 4,151,639 T→C H487R (CAC→CGC)  ppc ← phosphoenolpyruvate carboxylase
RA 4,156,962 T→C V38A (GTG→GCG)  argH → argininosuccinate lyase
RA 4,267,564 T→C Y151H (TAT→CAT)  tyrB → tyrosine aminotransferase
RA 4,281,343 A→G E455G (GAG→GGG)  yjcE → putative transporter YjcE
RA 4,282,253 T→C S275G (AGC→GGC)  yjcF ← pentapeptide repeat‑containing protein YjcF
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,320,725 T→A I175F (ATC→TTC)  phnH ← carbon‑phosphorus lyase core complex subunit PhnH
RA 4,359,475 C→T G186D (GGT→GAT)  cadB ← lysine:cadaverine antiporter
RA 4,369,492 T→C A307A (GCA→GCG yjeH ← L‑methionine/branched chain amino acid exporter
RA 4,372,554 G→A V510V (GTG→GTA groL → chaperonin GroEL
RA 4,381,225 T→A D365V (GAT→GTT)  frdA ← fumarate reductase flavoprotein subunit
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,511,516 A→G V300A (GTG→GCG)  fecD ← ferric citrate ABC transporter membrane subunit FecD
RA 4,541,175 (A)7→6 coding (219/603 nt) fimB → regulator for fimA
RA 4,563,534 A→G C24R (TGT→CGT)  yjiK ← uncharacterized protein YjiK
RA 4,586,296 G→A Q156* (CAG→TAG)  hsdR ← type I restriction enzyme EcoKI endonuclease component
RA 4,591,444 C→T intergenic (‑165/‑213) btsT ← / → tsr pyruvate:H(+) symporter/methyl‑accepting chemotaxis protein Tsr
RA 4,593,136 T→C S494P (TCT→CCT)  tsr → methyl‑accepting chemotaxis protein Tsr
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,632 G→A intergenic (‑231/+37) leuQ ← / ← rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
RA 4,609,336 (C)7→8 intergenic (+13/‑78) yjjG → / → prfC pyrimidine nucleotidase/peptide chain release factor RF3
RA 4,632,628 A→G K632K (AAA→AAG slt → soluble lytic murein transglycosylase
RA 4,633,810 A→G Y5C (TAC→TGC)  ytjC → putative phosphatase
RA 4,634,286 T→C L164L (TTA→CTA)  ytjC → putative phosphatase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 433032 433094 63 4 [2] [2] 3 nrdR NrdR transcriptional repressor
* * ÷ NC_000913 732112–732285 732344 60–233 3 [2] [2] 4 rhsC rhs element protein RhsC
* * ÷ NC_000913 1430004 1430094 91 3 [2] [1] 3 stfR Rac prophage; putative membrane protein
* * ÷ NC_000913 2521087–2521121 2521185–2521183 63–99 3 [2] [2] 4 [valX]–[valY] [valX],[valY]
* * ÷ NC_000913 3423784–3424230 3424522–3424240 11–739 3 [2] [1] 4 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3762280–3764065 3765177–3764077 13–2898 3 [2] [2] 3 rhsA rhs element protein RhsA
* * ÷ NC_000913 3942162–3943392 3946636–3946229 2838–4475 4 [1] [2] 3 [rrsC]–rrlC [rrsC],gltU,rrlC

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =33 (1.140)6 (0.240) 6/232 1.7 20.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 19 (0.750)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =83 (2.860)47 (1.630) 37/262 0.0 53.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)33 (1.140) 27/264 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA