breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 8,964 A→G T243A (ACC→GCC)  talB → transaldolase B
RA 13,436 T→C V425A (GTG→GCG)  dnaK → chaperone protein DnaK
RA 40,989 C→T G315S (GGC→AGC)  caiT ← L‑carnitine:gamma‑butyrobetaine antiporter
RA 53,721 A→G F328L (TTC→CTC)  surA ← peptidyl‑prolyl cis‑trans isomerase SurA
RA 59,658 A→G intergenic (+379/+29) yabQ → / ← rluA protein YabQ/23S rRNA pseudouridine(746) and tRNA pseudouridine(32) synthase
RA 77,723 A→G T35A (ACA→GCA)  setA → sugar exporter SetA
RA 101,339 T→C V192A (GTC→GCC)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 156,750 A→G V45A (GTT→GCT)  yadN ← putative fimbrial protein YadN
RA 163,992 G→A A630T (GCG→ACG)  hrpB → putative ATP‑dependent RNA helicase HrpB
RA 189,571 A→G intergenic (‑65/‑141) map ← / → tff methionine aminopeptidase/putative small RNA T44
RA 200,287 A→G Y787C (TAC→TGC)  bamA → outer membrane protein assembly factor BamA
RA 237,222 A→G intergenic (+215/‑113) aspV → / → yafT tRNA‑Asp/lipoprotein YafT
RA 249,530 (A)5→4 pseudogene (541/1713 nt) lfhA ← flagellar system protein, promoterless fragment
RA 250,303 A→G pseudogene (232/756 nt) lafU → flagellar system protein, promoterless fragment
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 284,102 T→C V301A (GTG→GCG)  yagF → CP4‑6 prophage; D‑xylonate dehydratase
RA 291,942 A→G K148R (AAG→AGG)  yagJ → CP4‑6 prophage; protein YagJ
RA 300,444 T→C D164G (GAC→GGC)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 351,375 A→G Y54C (TAC→TGC)  prpD → 2‑methylcitrate dehydratase
RA 364,333 G→A A658V (GCG→GTG)  lacZ ← beta‑galactosidase
RA 374,326 G→A K153K (AAG→AAA mhpE → 4‑hydroxy‑2‑oxovalerate aldolase
RA 393,709 A→G pseudogene (740/1449 nt) yaiT → putative autotransporter YaiT
RA 395,726 A→G F188L (TTC→CTC)  ampH ← peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
RA 423,327 T→G C270W (TGT→TGG malZ → maltodextrin glucosidase
RA 445,425 A→G R414R (CGT→CGC yajR ← putative major facilitator superfamily transport protein YajR
RA 458,411 C→T P329L (CCG→CTG)  clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 461,030 C→T P715S (CCG→TCG)  lon → Lon protease
RA 473,618 A→G H218R (CAC→CGC)  amtB → ammonia/ammonium transporter
RA 513,935 (A)7→6 intergenic (+67/‑58) ybaT → / → cueR putative transporter YbaT/DNA‑binding transcriptional dual regulator CueR
RA 525,807 C→T T849T (ACC→ACT rhsD → protein RhsD
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 538,138 A→G N169S (AAC→AGC)  ybbW → putative allantoin transporter
RA 566,691 Δ1 bp pseudogene (8/93 nt) renD → DLP12 prophage; protein RenD
RA 584,251 T→C Y191H (TAT→CAT)  appY → DNA‑binding transcriptional activator AppY
RA 584,320 A→G S214G (AGT→GGT)  appY → DNA‑binding transcriptional activator AppY
RA 588,641 A→G Y772H (TAT→CAT)  nfrA ← bacteriophage N4 receptor, outer membrane protein
RA 588,928 A→G L676P (CTT→CCT)  nfrA ← bacteriophage N4 receptor, outer membrane protein
RA 700,329 A→G intergenic (‑3/+45) umpH ← / ← nagC UMP phosphatase/DNA‑binding transcriptional dual regulator NagC
RA 715,242 (T)6→5 intergenic (+44/‑170) pgm → / → ybfP phosphoglucomutase/lipoprotein YbfP
RA 723,528 (T)8→7 coding (887/2685 nt) kdpD ← sensory histidine kinase KdpD
RA 731,616 C→T H678H (CAC→CAT rhsC → rhs element protein RhsC
RA 786,717 C→T intergenic (+32/+126) aroG → / ← gpmA 3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive/2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
RA 790,895 T→C L45L (TTA→TTG galT ← galactose‑1‑phosphate uridylyltransferase
RA 803,566 C→T H22Y (CAT→TAT)  ybhJ → putative hydratase YbhJ
RA 821,842 (C)5→4 coding (657/957 nt) ybhN ← conserved inner membrane protein YbhN
RA 824,458 T→C T14A (ACC→GCC)  ybhP ← endonuclease/exonuclease/phosphatase domain‑containing protein YbhP
RA 824,547 C→T intergenic (‑50/‑83) ybhP ← / → ybhQ endonuclease/exonuclease/phosphatase domain‑containing protein YbhP/putative inner membrane protein
RA 833,631 A→G S188G (AGT→GGT)  dinG → ATP‑dependent DNA helicase DinG
RA 840,048 A→G V495A (GTG→GCG)  fiu ← putative iron siderophore outer membrane transporter
RA 842,639 C→T A103V (GCG→GTG)  rlmF → 23S rRNA m(6)A1618 methyltransferase
RA 847,173 C→T intergenic (‑54/+85) glnP ← / ← glnH L‑glutamine ABC transporter membrane subunit/L‑glutamine ABC transporter periplasmic binding protein
RA 868,422 (C)8→9 coding (903/1872 nt) gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 878,179 A→G intergenic (‑144/‑69) rimO ← / → bssR ribosomal protein S12 methylthiotransferase RimO/regulator of biofilm formation
RA 879,527 A→G S262S (TCA→TCG yliI → aldose sugar dehydrogenase YliI
RA 883,336 T→C D18G (GAC→GGC)  ybjG ← undecaprenyl pyrophosphate phosphatase
RA 884,549 T→C W293R (TGG→CGG)  mdfA → multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 890,085 A→G intergenic (‑266/‑4) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 913,656 A→G R53R (CGT→CGC hcp ← protein S‑nitrosylase
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 933,596 A→G T125A (ACC→GCC)  ftsK → cell division DNA translocase FtsK
RA 941,535 T→C C193R (TGC→CGC)  dmsA → dimethyl sulfoxide reductase subunit A
RA 951,214 C→T intergenic (‑134/+58) pflA ← / ← pflB pyruvate formate‑lyase activating enzyme/pyruvate formate‑lyase
RA 952,388 A→G A389A (GCT→GCC pflB ← pyruvate formate‑lyase
RA 964,208 A→G intergenic (+96/‑112) ihfB → / → ycaI integration host factor subunit beta/conserved inner membrane protein YcaI
RA 1,004,160 (G)7→8 coding (214/711 nt) ycbF → putative fimbrial chaperone YcbF
RA 1,007,316 T→C T95T (ACA→ACG ycbX ← 6‑N‑hydroxylaminopurine resistance protein
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,017,078 A→G F408L (TTC→CTC)  ycbZ ← putative ATP‑dependent protease YcbZ
RA 1,020,576 A→G V115A (GTG→GCG)  sulA ← cell division inhibitor SulA
RA 1,048,161 (G)5→6 coding (531/747 nt) gfcC ← capsule biosynthesis GfcC family protein
RA 1,055,339 A→G S280S (AGT→AGC torS ← sensory histidine kinase TorS
RA 1,066,854 (G)5→6 intergenic (+28/+10) agp → / ← yccJ glucose‑1‑phosphatase/PF13993 family protein YccJ
RA 1,076,112 C→T C924Y (TGC→TAC)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,076,565 A→G V773A (GTG→GCG)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,092,272 (T)5→4 coding (18/2424 nt) pgaA ← partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine outer membrane porin
RA 1,097,580 (G)7→8 noncoding (73/88 nt) serX ← tRNA‑Ser
RA 1,099,236 A→G T120A (ACT→GCT)  ycdX → zinc‑binding phosphatase
RA 1,133,166 T→C V198A (GTC→GCC)  flgE → flagellar hook protein FlgE
RA 1,140,058 A→G Y9C (TAC→TGC)  flgL → flagellar hook‑filament junction protein 2
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,172,696 A→G R423R (CGT→CGC mfd ← transcription‑repair coupling factor
RA 1,182,367 A→G W155R (TGG→CGG)  potD ← spermidine preferential ABC transporter periplasmic binding protein
RA 1,184,982 T→C T205A (ACT→GCT)  potA ← spermidine preferential ABC transporter ATP binding subunit
RA 1,194,643 T→C K103K (AAA→AAG rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,266,530 T→C V146A (GTC→GCC)  prmC → protein‑(glutamine‑N(5)) methyltransferase
RA 1,272,642 C→T P46S (CCG→TCG)  chaC → glutathione‑specific gamma‑glutamylcyclotransferase
RA 1,276,107 A→G R504R (CGT→CGC narX ← sensory histidine kinase NarX
RA 1,297,154 A→G V323A (GTG→GCG)  adhE ← aldehyde‑alcohol dehydrogenase
RA 1,303,697 G→A A267T (GCA→ACA)  oppB → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppB
RA 1,304,226 T→C G131G (GGT→GGC oppC → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppC
RA 1,351,196 (G)7→6 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,361,393 C→T R92C (CGT→TGT)  puuD → gamma‑glutamyl‑gamma‑aminobutyrate hydrolase
RA 1,377,819 T→C Y330H (TAC→CAC)  ycjS → putative oxidoreductase YcjS
RA 1,438,207 A→G Y860H (TAT→CAT)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,438,306 A→G Y827H (TAT→CAT)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,455,822 G→A G220D (GGT→GAT)  paaC → phenylacetyl‑CoA 1,2‑epoxidase, structural subunit
RA 1,459,273 A→G I74V (ATC→GTC)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase
RA 1,462,242 T→C Y40H (TAT→CAT)  paaK → phenylacetate‑CoA ligase
RA 1,479,687 (G)6→5 coding (550/1758 nt) ynbC → hydrolase/methyltransferase domain‑containing protein YnbC
RA 1,491,397 A→G T166A (ACA→GCA)  cybB → cytochrome b561
RA 1,496,361 G→A A358A (GCG→GCA ydcJ → DUF1338 domain‑containing protein YdcJ
RA 1,502,388 (T)7→8 intergenic (+233/‑69) tehB → / → ydcL tellurite methyltransferase/DUF3313 domain‑containing lipoprotein YdcL
RA 1,518,660 T→C T63A (ACG→GCG)  mnaT ← L‑amino acid N‑acyltransferase
RA 1,519,178 A→G D51G (GAC→GGC)  curA → NADPH‑dependent curcumin/dihydrocurcumin reductase
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,552,034 C→T intergenic (+43/+364) fdnI → / ← yddM formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM
RA 1,560,788 C→T A48T (GCT→ACT)  ddpB ← putative D,D‑dipeptide ABC transporter membrane subunit DdpB
RA 1,584,934 (T)7→8 coding (1553/2280 nt) ydeP ← putative oxidoreductase YdeP
RA 1,594,132 (C)8→7 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,594,256 (C)8→9 pseudogene (3831/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,595,669 A→G pseudogene (2418/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,597,873 T→C pseudogene (214/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,606,758 T→C V220A (GTA→GCA)  lsrF → 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase
RA 1,622,793 A→G intergenic (+52/+24) mgtS → / ← mgrR small protein MgtS/small regulatory RNA MgrR
RA 1,635,527 A→G G107G (GGT→GGC ydfN ← Qin prophage; putative side tail fiber assembly protein
RA 1,644,058 A→G S83P (TCC→CCC)  ydfU ← Qin prophage; protein YdfU
RA 1,653,981 T→C E387E (GAA→GAG rspA ← mandelate racemase/muconate lactonizing enzyme family protein RspA
RA 1,664,627 G→A G41D (GGC→GAC)  dmsD → redox enzyme maturation protein DmsD
RA 1,680,569 A→G N338S (AAC→AGC)  ydgI → putative arginine:ornithine antiporter
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,720,731 T→C T32A (ACC→GCC)  slyA ← DNA‑binding transcriptional dual regulator SlyA
RA 1,735,088 A→G G218G (GGA→GGG mepH → peptidoglycan DD‑endopeptidase MepH
RA 1,735,184 T→C S250S (AGT→AGC mepH → peptidoglycan DD‑endopeptidase MepH
RA 1,765,953 T→C E911E (GAA→GAG ydiJ ← putative FAD‑linked oxidoreductase
RA 1,769,258 G→A R62H (CGC→CAC)  ydiK → putative transporter YdiK
RA 1,769,478 (G)7→8 coding (405/1113 nt) ydiK → putative transporter YdiK
RA 1,785,140 A→G V658A (GTT→GCT)  ppsA ← phosphoenolpyruvate synthetase
RA 1,787,826 Δ1 bp coding (382/834 nt) ppsR → posphoenolpyruvate synthetase regulatory protein
RA 1,792,029 T→C intergenic (‑9/+238) ydiV ← / ← nlpC anti‑FlhDC factor/NlpC/P60 family lipoprotein NlpC
RA 1,806,684 T→C R105R (CGT→CGC pfkB → 6‑phosphofructokinase II
RA 1,829,570 C→T R55R (CGG→CGA astD ← aldehyde dehydrogenase
RA 1,835,101 C→T G98G (GGC→GGT ydjZ → DedA family protein YdjZ
RA 1,863,508 T→C R246R (CGT→CGC gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,874,785 T→C V145A (GTG→GCG) 
P264P (CCA→CCG
yeaL →
nimR ←
conserved inner membrane protein YeaL
DNA‑binding transcriptional repressor NimR
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,900,630 A→C V319G (GTC→GGC)  yoaE ← putative inner membrane protein
RA 1,907,825 T→C intergenic (‑234/+436) yobF ← / ← yebO DUF2527 domain‑containing protein YobF/uncharacterized protein YebO
RA 1,927,241 (C)6→7 coding (1599/2061 nt) ptrB ← oligopeptidase B
RA 1,961,461 T→C V467A (GTA→GCA)  argS → arginine‑‑tRNA ligase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,982,680 G→A G287G (GGC→GGT araH ← arabinose ABC transporter membrane subunit
RA 2,006,068 A→G N119S (AAC→AGC)  fliT → flagellar biosynthesis protein FliT
RA 2,009,080 T→C S268S (AGT→AGC yedE → putative selenium transporter YedE
RA 2,017,228 T→C G225G (GGT→GGC fliI → flagellum‑specific ATP synthase FliI
RA 2,018,260 (A)8→9 coding (315/444 nt) fliJ → flagellar biosynthesis protein FliJ
RA 2,030,935 A→C R461R (CGT→CGG dcm ← DNA‑cytosine methyltransferase
RA 2,041,588 G→A G72S (GGC→AGC)  zinT → metal‑binding protein ZinT
RA 2,050,074 A→G S1713G (AGC→GGC)  yeeJ → inverse autotransporter adhesin
RA 2,060,312 A→G I201I (ATT→ATC cbl ← DNA‑binding transcriptional activator Cbl
RA 2,067,300 C→T G8R (GGA→AGA)  insH‑6 ← CP4‑44 prophage; IS5 transposase and trans‑activator
RA 2,074,419 C→T P961S (CCT→TCT)  flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,101,186 G→A T28I (ACC→ATC)  gnd ← 6‑phosphogluconate dehydrogenase, decarboxylating
RA 2,136,843 A→G V134A (GTC→GCC)  wza ← outer membrane polysaccharide export protein Wza
RA 2,158,591 G→A R69H (CGC→CAC)  mdtC → multidrug efflux pump RND permease subunit MdtC
RA 2,168,791 T→C L27P (CTG→CCG)  yegS → lipid kinase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,183,713 T→C intergenic (‑62/+3) yegX ← / ← thiD putative hydrolase/bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
RA 2,188,888 T→C N675D (AAT→GAT)  yehB ← putative fimbrial usher protein YehB
RA 2,188,957 (C)5→4 coding (1954/2481 nt) yehB ← putative fimbrial usher protein YehB
RA 2,198,192 T→C intergenic (+894/‑2087) yehF → / → yehI putative protein YehF/DUF4132 domain‑containing protein YehI
RA 2,206,807 A→G Q371Q (CAA→CAG yehM → putative YehN
RA 2,208,064 A→G D33G (GAC→GGC)  yehP → VWA domain‑containing protein YehP
RA 2,214,548 A→G L33L (TTA→CTA)  btsS ← high‑affinity pyruvate receptor
RA 2,223,019 T→C F279L (TTT→CTT)  dld → D‑lactate dehydrogenase
RA 2,233,384 A→G V169V (GTA→GTG sanA → DUF218 domain‑containing protein SanA
RA 2,234,059 A→G E9E (GAA→GAG preT → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
RA 2,269,340 C→T R163C (CGT→TGT)  lpxT → Kdo2‑lipid A phosphotransferase
RA 2,329,418 T→C Q127R (CAA→CGA)  yfaQ ← tandem DUF2300 domain‑containing protein YfaQ
RA 2,352,275 T→C I34V (ATA→GTA)  glpT ← sn‑glycerol 3‑phosphate:phosphate antiporter
RA 2,361,874 T→C Y113C (TAC→TGC)  yfaX ← putative DNA‑binding transcriptional regulator YfaX
RA 2,408,713 G→A V385I (GTC→ATC)  alaA → glutamate‑‑pyruvate aminotransferase AlaA
RA 2,412,679 A→G *152Q (TAA→CAA)  yfbV ← UPF0208 membrane protein YfbV
RA 2,446,285 C→T A34T (GCA→ACA)  mepA ← peptidoglycan DD‑endopeptidase/peptidoglycan LD‑endopeptidase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,457,618 T→C E514E (GAA→GAG fadJ ← 3‑hydroxyacyl‑CoA dehydrogenase FadJ
MC JC 2,468,599 Δ4 bp coding (386‑389/921 nt) yfdH → CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
RA 2,516,856 G→A A326V (GCG→GTG)  pdeA ← putative c‑di‑GMP phosphodiesterase PdeA
RA 2,517,911 C→G intergenic (‑79/+130) pdeA ← / ← alaX putative c‑di‑GMP phosphodiesterase PdeA/tRNA‑Ala
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,531,391 G→A intergenic (‑158/‑72) zipA ← / → cysZ cell division protein ZipA/sulfate:H(+) symporter
RA 2,621,871 G→A P225P (CCG→CCA purM → phosphoribosylformylglycinamide cyclo‑ligase
RA 2,622,361 T→C L43P (CTT→CCT)  purN → phosphoribosylglycinamide formyltransferase 1
RA 2,662,372 G→A intergenic (‑241/+211) iscR ← / ← trmJ DNA‑binding transcriptional dual regulator IscR/tRNA Cm32/Um32 methyltransferase
RA 2,693,342 G→A I734I (ATC→ATT purL ← phosphoribosylformylglycinamide synthetase
RA 2,734,543 (C)8→9 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,787,637 T→C pseudogene (1241/2253 nt) ygaQ → putative uncharacterized protein YgaQ
RA 2,803,347 (T)7→6 coding (2000/2145 nt) nrdE → ribonucleoside‑diphosphate reductase 2 subunit alpha
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,818,463 A→G intergenic (‑189/+10) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,826,424 T→C G11G (GGT→GGC srlE → sorbitol‑specific PTS enzyme IIBC1 component
RA 2,828,221 T→G V162G (GTC→GGC)  srlD → sorbitol‑6‑phosphate 2‑dehydrogenase
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,862,139 +C coding (710/909 nt) ygbJ → putative L‑threonate dehydrogenase
RA 2,875,776 G→A P185L (CCG→CTG)  cysD ← sulfate adenylyltransferase subunit 2
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,964,837 G→A R107C (CGT→TGT)  thyA ← thymidylate synthase
RA 2,983,325 T→C D267G (GAT→GGT)  kduI ← 5‑dehydro‑4‑deoxy‑D‑glucuronate isomerase
RA 3,000,657 G→A A105T (GCC→ACC)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,003,479 (G)6→7 coding (866/879 nt) xdhB → putative xanthine dehydrogenase FAD‑binding subunit XdhB
RA 3,048,383 A→G V220A (GTA→GCA)  gcvP ← glycine decarboxylase
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,099,460 (T)5→4 coding (106/1008 nt) yggM ← DUF1202 domain‑containing protein YggM
RA 3,125,588 G→A A312V (GCC→GTC)  glcE ← glycolate dehydrogenase, putative FAD‑binding subunit
RA 3,148,203 T→C W103R (TGG→CGG)  gpr → L‑glyceraldehyde 3‑phosphate reductase
RA 3,160,621 A→G S176P (TCC→CCC)  ygiQ ← radical SAM superfamily protein YgiQ
RA 3,195,773 T→C E327E (GAA→GAG rfaE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
RA 3,208,795 T→C Y258H (TAC→CAC)  ttdT → L‑tartrate:succinate antiporter
RA 3,240,945 T→C A334A (GCT→GCC sstT → serine/threonine:Na(+) symporter
RA 3,245,342 T→C L40S (TTA→TCA)  exuT → hexuronate transporter
RA 3,267,000 A→G G22G (GGT→GGC tdcA ← DNA‑binding transcriptional activator TdcA
RA 3,272,178 T→C T192A (ACT→GCT)  garR ← tartronate semialdehyde reductase
RA 3,298,828 T→C T7A (ACA→GCA)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase
RA 3,301,080 T→C D66G (GAC→GGC)  yhbT ← SCP2 domain‑containing protein YhbT
RA 3,306,288 A→G F525L (TTT→CTT)  deaD ← ATP‑dependent RNA helicase DeaD
RA 3,328,195 (T)5→4 intergenic (+112/+44) yhbY → / ← greA ribosome assembly factor YhbY/transcription elongation factor GreA
RA 3,351,038 G→A A663V (GCA→GTA)  arcB ← sensory histidine kinase ArcB
RA 3,352,281 A→G Y249H (TAC→CAC)  arcB ← sensory histidine kinase ArcB
RA 3,353,504 G→A H183Y (CAC→TAC)  yhcC ← radical SAM family oxidoreductase YhcC
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,358,654 T→C R1310R (CGT→CGC gltB → glutamate synthase subunit GltB
RA 3,362,973 (T)6→7 coding (167/2382 nt) yhcD → putative fimbrial usher protein YhcD
RA 3,367,774 (C)6→7 intergenic (+132/‑53) yhcF → / → yhcG DUF1120 domain‑containing protein YhcF/DUF1016 domain‑containing protein YhcG
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,423,614 A→G noncoding (42/76 nt) thrV ← tRNA‑Thr
RA 3,462,844 (G)6→7 coding (501/588 nt) gspJ → Type II secretion system protein GspJ
RA 3,514,258 T→C E44E (GAA→GAG gph ← phosphoglycolate phosphatase
RA 3,517,581 T→C L302L (TTA→TTG aroB ← 3‑dehydroquinate synthase
RA 3,518,075 A→G S138P (TCC→CCC)  aroB ← 3‑dehydroquinate synthase
RA 3,525,346 A→G T826A (ACG→GCG)  mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,595,404 A→G V26A (GTG→GCG)  livM ← branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM
RA 3,629,222 A→G V106A (GTG→GCG)  rbbA ← ribosome‑associated ATPase
RA 3,643,610 C→T G525S (GGC→AGC)  prlC ← oligopeptidase A
RA 3,681,795 (G)6→7 coding (146/1497 nt) yhjJ ← peptidase M16 family protein
RA 3,701,625 A→G T355A (ACG→GCG)  yhjV → putative transporter YhjV
RA 3,712,926 (T)8→7 coding (9/699 nt) yhjY ← conserved protein YhjY
RA 3,779,794 T→C G247G (GGT→GGC lldR → DNA‑binding transcriptional dual regulator LldR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,834,428 (G)6→7 coding (110/2319 nt) yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,836,534 (C)6→7 intergenic (+218/‑419) selC → / → setC tRNA‑Sec/putative arabinose exporter
RA 3,839,499 T→C L165L (TTA→TTG nlpA ← lipoprotein‑28
RA 3,840,749 T→C T331A (ACC→GCC)  nepI ← purine ribonucleoside exporter
RA 3,844,614 T→C G217G (GGT→GGC adeD → adenine deaminase
RA 3,850,448 (C)5→4 coding (279/591 nt) uhpA ← DNA‑binding transcriptional activator UhpA
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,873,113 T→C D167G (GAC→GGC)  dgoA ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA 3,875,866 T→C C143C (TGT→TGC yidX → putative lipoprotein YidX
RA 3,902,076 (T)5→6 coding (145/1617 nt) bglH ← carbohydrate‑specific outer membrane porin, cryptic
RA 3,908,697 T→C N248S (AAC→AGC)  pstA ← phosphate ABC transporter membrane subunit PstA
RA 3,917,574 C→T G231D (GGC→GAC)  atpG ← ATP synthase F1 complex subunit gamma
RA 3,925,443 T→C D64G (GAT→GGT)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 4,012,958 T→C intergenic (‑142/‑95) metR ← / → metE DNA‑binding transcriptional dual regulator MetR/cobalamin‑independent homocysteine transmethylase
RA 4,051,328 G→A intergenic (+48/‑19) csrC → / → yihI small regulatory RNA CsrC/Der GTPase‑activating protein YihI
RA 4,058,009 A→G M9T (ATG→ACG)  glnA ← glutamine synthetase
RA 4,060,280 (T)9→8 intergenic (+50/‑167) bipA → / → yihL ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL
RA 4,074,623 A→G S295S (TCA→TCG yihV → 6‑deoxy‑6‑sulfofructose kinase
RA 4,074,728 C→T A20A (GCC→GCT yihW → putative DNA‑binding transcriptional regulator YihW
RA 4,093,113 A→G intergenic (‑290/+11) frvA ← / ← rhaM putative PTS enzyme IIA component FrvA/L‑rhamnose mutarotase
RA 4,094,673 G→A intergenic (‑401/+50) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,094,683 C→T intergenic (‑411/+40) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,103,388 C→T H189H (CAC→CAT yiiM → 2‑amino‑6‑N‑hydroxylaminopurine resistance protein
JC JC 4,118,824 IS4 (+) +12 bp intergenic (+64/+10) zapB → / ← rraA cell division factor ZapB/ribonuclease E inhibitor protein A
RA 4,137,062 T→C H199R (CAT→CGT)  yijF ← conserved protein YijF
RA 4,180,277 T→C R94R (CGT→CGC rplJ → 50S ribosomal subunit protein L10
RA 4,187,647 T→C G766G (GGT→GGC rpoC → RNA polymerase subunit beta'
RA 4,194,999 A→G H367H (CAT→CAC thiC ← phosphomethylpyrimidine synthase
RA 4,197,289 (C)5→4 coding (387/774 nt) nudC → NADH pyrophosphatase
RA 4,201,027 A→G R154R (AGA→AGG yjaH → DUF1481 domain‑containing protein YjaH
RA 4,201,888 T→C intergenic (‑200/‑38) zraP ← / → zraS zinc responsive, periplasmic protein with chaperone activity/sensory histidine kinase ZraS
RA 4,224,209 T→C Y128H (TAC→CAC)  metH → cobalamin‑dependent methionine synthase
RA 4,271,224 T→C N883S (AAC→AGC)  uvrA ← excision nuclease subunit A
RA 4,277,950 T→C noncoding (117/140 nt) ryjA ← small RNA RyjA
RA 4,293,106 (G)7→8 coding (1595/1659 nt) nrfE → putative cytochrome c‑type biogenesis protein NrfE
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,303,598 A→G V171A (GTA→GCA)  mdtN ← putative multidrug efflux pump membrane fusion protein
RA 4,310,361 T→C E207G (GAA→GGA)  alsA ← D‑allose ABC transporter ATP binding subunit
RA 4,322,651 A→G pseudogene (631/839 nt) phnE ← phosphonate ABC transporter membrane subunit
RA 4,365,262 A→G L32P (CTG→CCG)  cutA ← copper binding protein CutA
RA 4,381,782 G→A G179G (GGC→GGT frdA ← fumarate reductase flavoprotein subunit
RA 4,392,811 A→G intergenic (+153/‑81) glyY → / → yjeV tRNA‑Gly/uncharacterized protein YjeV
RA 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA 2'‑O‑ribose G2251 methyltransferase/conserved protein YjfI
RA 4,423,375 A→G N177S (AAT→AGT)  ulaE → L‑ribulose‑5‑phosphate 3‑epimerase UlaE
RA 4,429,721 A→G intergenic (+166/‑143) fklB → / → cycA FKBP‑type peptidyl‑prolyl cis‑trans isomerase FklB/serine/alanine/glycine/cycloserine:H(+)symporter
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,451,646 A→G S197G (AGC→GGC)  ytfR → galactofuranose ABC transporter putative ATP binding subunit
RA 4,492,583 (G)6→7 intergenic (‑58/+4) idnT ← / ← idnO L‑idonate/5‑ketogluconate/gluconate transporter/5‑keto‑D‑gluconate 5‑reductase
RA 4,510,046 A→G pseudogene (243/330 nt) yjhV → KpLE2 phage‑like element; putative uncharacterized protein YjhV
RA 4,514,148 A→G V54A (GTG→GCG)  fecB ← ferric citrate ABC transporter periplasmic binding protein
RA 4,514,423 T→C D752G (GAC→GGC)  fecA ← ferric citrate outer membrane transporter
RA 4,514,875 G→A S601S (TCC→TCT fecA ← ferric citrate outer membrane transporter
RA 4,562,396 A→G L94L (TTG→CTG)  yjiJ ← putative transporter YjiJ
RA 4,567,017 A→G I77T (ATC→ACC)  yjiN ← DUF445 domain‑containing protein YjiN
RA 4,568,451 A→G F23F (TTT→TTC mdtM ← multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 4,577,861 A→G I33T (ATA→ACA)  mcrC ← 5‑methylcytosine‑specific restriction enzyme subunit McrC
RA 4,578,851 A→G S163P (TCA→CCA)  mcrB ← 5‑methylcytosine‑specific restriction enzyme subunit McrB
RA 4,593,220 C→T R522C (CGT→TGT)  tsr → methyl‑accepting chemotaxis protein Tsr
RA 4,595,207 A→G D215D (GAT→GAC lgoR ← putative DNA‑binding transcriptional regulator LgoR
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,636,586 A→G T194A (ACC→GCC)  creB → DNA‑binding transcriptional regulator CreB
RA 4,637,743 (A)9→8 coding (1048/1425 nt) creC → sensory histidine kinase CreC

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 227116–228673 228889–228689 17–1774 11 [9] [9] 10 [rrlH]–rrfH [rrlH],rrfH
* * ÷ NC_000913 1097071–1097157 1097562–1097163 7–492 10 [9] [9] 10 ycdU/serX uncharacterized protein YcdU/tRNA‑Ser
* * ÷ NC_000913 1196280 1211417 15138 10 [9] [5] 10 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1552134–1552184 1552235–1552213 30–102 10 [8] [9] 12 fdnI/yddM formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM
* * ÷ NC_000913 1633338–1634066 1634907–1634098 33–1570 11 [9] [9] 10 pinQ–[ydfN] pinQ,tfaQ,[ydfN]
* * ÷ NC_000913 3423776–3424231 3424523–3424239 9–748 10 [9] [6] 10 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374963NA (NA)7 (0.210) 7/190 2.2 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 12994980 (0.000)59 (1.240) 41/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =86 (1.750)111 (2.270) 55/274 0.0 72.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)56 (1.140) 43/276 0.1 93.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.080)coding (3907/4134 nt) rhsA rhs element protein RhsA