breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 28,427 C→T G18G (GGC→GGT dapB → 4‑hydroxy‑tetrahydrodipicolinate reductase
RA 31,826 A→G N337S (AAC→AGC)  carB → carbamoyl phosphate synthetase subunit beta
RA 34,120 (T)9→8 intergenic (+82/‑180) carB → / → caiF carbamoyl phosphate synthetase subunit beta/DNA‑binding transcriptional activator CaiF
RA 39,838 C→T W183* (TGG→TGA caiA ← crotonobetainyl‑CoA reductase
RA 61,202 A→G L688P (CTG→CCG)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
RA 62,678 A→G L196P (CTC→CCC)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
RA 62,681 A→G L195P (CTG→CCG)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
RA 66,691 C→T intergenic (‑141/+144) araD ← / ← araA L‑ribulose‑5‑phosphate 4‑epimerase AraD/L‑arabinose isomerase
RA 71,488 A→G G46G (GGA→GGG yabI → DedA family protein YabI
RA 83,579 (A)7→8 intergenic (‑50/+43) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 93,440 A→G E92G (GAG→GGG)  murE → UDP‑N‑acetylmuramoyl‑L‑alanyl‑D‑glutamate‑‑2, 6‑diaminopimelate ligase
RA 130,378 A→G Y295H (TAC→CAC)  yacH ← DUF3300 domain‑containing protein YacH
RA 144,977 (A)7→8 coding (401/441 nt) yadI → putative PTS enzyme IIA component YadI
RA 166,118 A→G K463K (AAA→AAG mrcB → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
RA 166,897 T→C V723A (GTC→GCC)  mrcB → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB
RA 189,107 A→G Y134H (TAC→CAC)  map ← methionine aminopeptidase
RA 243,207 A→G W33R (TGG→CGG)  fadE ← acyl‑CoA dehydrogenase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 272,712 (G)5→6 intergenic (+58/‑135) eyeA → / → ykfC CP4‑6 prophage; small RNA EyeA/CP4‑6 prophage; PF00078 domain‑containing protein YkfC
RA 281,529 T→C H152R (CAC→CGC)  yagA ← CP4‑6 prophage; integrase core domain‑containing protein YagA
RA 325,411 A→G intergenic (‑47/+166) ykgH ← / ← betA uncharacterized protein YkgH/choline dehydrogenase
RA 331,199 A→G G579G (GGA→GGG betT → choline:H(+) symporter
RA 352,317 A→C D368A (GAC→GCC)  prpD → 2‑methylcitrate dehydratase
RA 355,454 G→A G178D (GGC→GAC)  codB → cytosine transporter
RA 375,420 C→T intergenic (+539/‑39) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 380,021 (G)10→8 intergenic (‑140/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 388,948 A→G R260R (CGT→CGC hemB ← porphobilinogen synthase
RA 400,486 A→G R146R (CGT→CGC ddlA ← D‑alanine‑‑D‑alanine ligase A
RA 413,449 A→G F769L (TTC→CTC)  sbcC ← ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
RA 422,427 (C)5→4 intergenic (+68/‑91) proY → / → malZ putative transporter ProY/maltodextrin glucosidase
RA 453,249 A→G G99G (GGT→GGC ampG ← muropeptide:H(+) symporter
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 534,368 T→C R151R (CGT→CGC gcl → glyoxylate carboligase
RA 538,610 G→A A326A (GCG→GCA ybbW → putative allantoin transporter
RA 545,990 T→C Q125R (CAG→CGG)  allD ← ureidoglycolate dehydrogenase
RA 592,390 A→G S263S (AGT→AGC nfrB ← bacteriophage N4 receptor, inner membrane subunit
RA 602,962 T→C V335A (GTC→GCC)  pheP → phenylalanine:H(+) symporter PheP
RA 617,433 T→C S1093P (TCG→CCG)  entF → apo‑serine activating enzyme
RA 640,591 A→G Y280C (TAC→TGC)  ahpF → alkyl hydroperoxide reductase, AhpF component
RA 640,716 A→G T322A (ACC→GCC)  ahpF → alkyl hydroperoxide reductase, AhpF component
RA 645,367 A→G M405T (ATG→ACG)  citT ← citrate:succinate antiporter
RA 657,830 T→C T37A (ACG→GCG)  crcB ← F(‑) channel
RA 669,978 G→A P199S (CCG→TCG)  nadD ← nicotinate‑mononucleotide adenylyltransferase
RA 679,673 A→G T56A (ACT→GCT)  ybeU → DUF1266 domain‑containing protein YbeU
RA 685,941 (C)6→7 coding (729/741 nt) gltJ ← glutamate/aspartate ABC transporter membrane subunit GltJ
RA 689,053 A→G intergenic (‑40/+290) insH‑3 ← / ← lnt IS5 transposase and trans‑activator/apolipoprotein N‑acyltransferase
RA 710,861 A→G intergenic (‑136/+74) uof ← / ← fldA RyhB‑regulated fur leader peptide/flavodoxin 1
RA 717,125 (G)6→8 coding (1141/1320 nt) potE ← putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
RA 721,836 C→T G860E (GGG→GAG)  kdpD ← sensory histidine kinase KdpD
RA 757,649 C→T F581F (TTC→TTT sdhA → succinate:quinone oxidoreductase, FAD binding protein
RA 771,282 A→G intergenic (+671/‑176) mngB → / → cydA alpha‑mannosidase/cytochrome bd‑I ubiquinol oxidase subunit I
RA 789,358 C→T V208I (GTC→ATC)  galK ← galactokinase
RA 825,051 G→A A353V (GCC→GTC)  ybhR ← putative ABC exporter membrane subunit YbhR
RA 832,736 A→G M72T (ATG→ACG)  ybiA ← N‑glycosidase YbiA
RA 843,979 A→G L501P (CTA→CCA)  ybiO ← moderate conductance mechanosensitive channel
RA 856,822 (GC)5→4 coding (860‑861/1593 nt) ybiT → ABC transporter ATP‑binding protein YbiT
RA 859,010 T→C I18V (ATC→GTC)  ybiU ← DUF1479 domain‑containing protein YbiU
RA 866,902 T→C L112P (CTG→CCG)  iaaA → isoaspartyl dipeptidase proenzyme
RA 868,422 (C)8→7 coding (903/1872 nt) gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 871,743 A→G G259G (GGA→GGG gsiC → glutathione ABC transporter membrane subunit GsiC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 910,426 A→G C208C (TGT→TGC poxB ← pyruvate oxidase
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 950,957 T→C T42A (ACC→GCC)  pflA ← pyruvate formate‑lyase activating enzyme
RA 961,476 T→C R92R (CGT→CGC cmk → cytidylate kinase
RA 975,737 A→G D39G (GAC→GGC)  mukE → chromosome partitioning protein MukE
RA 977,713 A→G Q463R (CAG→CGG)  mukB → chromosome partitioning protein MukB
RA 981,850 (G)6→5 coding (804/1848 nt) ldtD → L,D‑transpeptidase LdtD
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,029,739 (A)8→7 coding (231/1191 nt) rlmI ← 23S rRNA m(5)C1962 methyltransferase
RA 1,048,572 G→A G40G (GGC→GGT gfcC ← capsule biosynthesis GfcC family protein
RA 1,074,174 A→G intergenic (‑163/‑68) rutA ← / → rutR pyrimidine oxygenase/DNA‑binding transcriptional dual regulator RutR
RA 1,081,596 T→C pseudogene (241/830 nt) efeU → inactive ferrous iron permease EfeU, N‑terminal fragment
RA 1,104,948 (T)8→9 intergenic (+46/‑13) csgA → / → csgC curlin, major subunit/inhibitor of CsgA amyloid formation
RA 1,105,994 G→A D59N (GAT→AAT)  ymdB → 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
RA 1,108,666 A→G G92G (GGT→GGC opgC ← protein required for succinyl modification of osmoregulated periplasmic glucans
RA 1,113,194 Δ2 bp coding (2332‑2333/2544 nt) opgH → osmoregulated periplasmic glucans (OPGs) biosynthesis protein H
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,148,066 C→T A149V (GCC→GTC)  plsX → putative phosphate acyltransferase
RA 1,153,353 A→G A18A (GCA→GCG pabC → aminodeoxychorismate lyase
RA 1,155,557 T→C V145A (GTT→GCT)  tmk → hypothetical protein
RA 1,170,791 A→G R1058R (CGT→CGC mfd ← transcription‑repair coupling factor
RA 1,172,093 A→G R624R (CGT→CGC mfd ← transcription‑repair coupling factor
RA 1,185,933 C→T G30G (GGC→GGT pepT → peptidase T
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,230,806 A→G T14A (ACT→GCT)  umuD → DNA polymerase V protein UmuD
RA 1,252,660 T→C V532A (GTT→GCT)  dhaR → DNA‑binding transcriptional dual regulator DhaR
RA 1,262,453 A→G V142A (GTT→GCT)  ispE ← 4‑(cytidine 5'‑diphospho)‑2‑C‑methyl‑D‑erythritol kinase
RA 1,280,561 Δ1 bp coding (698/3744 nt) narG → nitrate reductase A subunit alpha
RA 1,298,105 A→G V6A (GTC→GCC)  adhE ← aldehyde‑alcohol dehydrogenase
RA 1,301,759 T→C V193A (GTG→GCG)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,346,840 (T)8→7 coding (45/105 nt) ymiB → putative protein YmiB
RA 1,365,076 A→G N282S (AAC→AGC)  puuB → gamma‑glutamylputrescine oxidase
RA 1,394,303 A→G E359E (GAA→GAG mppA → murein tripeptide ABC transporter periplasmic binding protein
RA 1,440,091 A→G Y232H (TAC→CAC)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,444,892 A→G G614G (GGA→GGG ydbH → PF11739 family protein YdbH
RA 1,453,575 T→C H23R (CAC→CGC)  paaZ ← oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,460,012 T→C V320A (GTT→GCT)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase
RA 1,464,032 A→G N165S (AAC→AGC)  paaX → DNA‑binding transcriptional repressor PaaX
RA 1,474,941 T→C W241R (TGG→CGG)  pdxI → pyridoxine 4‑dehydrogenase
RA 1,504,220 A→G K168K (AAA→AAG insQ → putative insertion element transposase InsQ
RA 1,518,956 G→A intergenic (‑110/‑71) mnaT ← / → curA L‑amino acid N‑acyltransferase/NADPH‑dependent curcumin/dihydrocurcumin reductase
RA 1,521,536 A→G G510G (GGT→GGC yncD ← putative TonB‑dependent outer membrane receptor
RA 1,525,336 (G)5→6 coding (645/1500 nt) ansP ← L‑asparagine transporter
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,555,703 G→A intergenic (‑34/+123) maeA ← / ← sra malate dehydrogenase, NAD‑requiring/30S ribosomal subunit protein S22
RA 1,558,766 T→C K84K (AAA→AAG ddpD ← putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpD
RA 1,565,696 (A)7→6 coding (38/2400 nt) dosP ← oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
RA 1,570,600 T→C intergenic (‑111/+45) gadC ← / ← gadB L‑glutamate:4‑aminobutyrate antiporter/glutamate decarboxylase B
RA 1,594,047 T→C pseudogene (4040/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,597,313 (C)7→8 pseudogene (774/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,642,961 T→C Y94C (TAC→TGC)  ydfT ← Qin prophage; putative antitermination protein Q
RA 1,661,680 G→A W375* (TGG→TGA ynfF → putative selenate reductase YnfF
RA 1,666,271 T→C G319G (GGT→GGC clcB → putative chloride:H(+) antiporter ClcB
RA 1,680,369 T→C G271G (GGT→GGC ydgI → putative arginine:ornithine antiporter
RA 1,682,372 T→C S72P (TCT→CCT)  rstA → DNA‑binding transcriptional regulator RstA
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,707,905 +C coding (987/2223 nt) rsxC → SoxR [2Fe‑2S] reducing system protein RsxC
RA 1,714,914 G→A A180T (GCA→ACA)  gstA → glutathione S‑transferase GstA
RA 1,733,200 A→G T1372A (ACG→GCG)  lhr → putative ATP‑dependent helicase Lhr
RA 1,760,153 T→C H124R (CAC→CGC)  sufS ← L‑cysteine desulfurase
RA 1,764,553 T→C intergenic (‑167/+160) sufA ← / ← rydB iron‑sulfur cluster insertion protein SufA/small regulatory RNA RydB
RA 1,765,674 (C)5→6 coding (3012/3057 nt) ydiJ ← putative FAD‑linked oxidoreductase
RA 1,766,131 T→C N852S (AAC→AGC)  ydiJ ← putative FAD‑linked oxidoreductase
RA 1,770,856 (T)6→5 coding (242/357 nt) ydiL → DUF1870 domain‑containing protein YdiL
RA 1,806,042 (C)6→7 coding (42/759 nt) ydiY ← acid‑inducible putative outer membrane protein YdiY
RA 1,818,550 (C)7→8 intergenic (‑50/+55) chbF ← / ← chbR monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR
RA 1,828,529 T→C G402G (GGA→GGG astD ← aldehyde dehydrogenase
RA 1,842,809 (T)5→6 coding (439/1344 nt) gdhA → glutamate dehydrogenase
RA 1,855,756 (T)7→8 coding (216/981 nt) ydjG ← NADH‑dependent methylglyoxal reductase
RA 1,858,955 (C)5→4 coding (1275/1380 nt) ydjK ← putative transporter YdjK
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,890,374 A→C G53G (GGT→GGG yeaY ← Slp family lipoprotein YeaY
RA 1,900,027 G→A intergenic (+2/+2) pdeD → / ← yoaE putative c‑di‑GMP phosphodiesterase PdeD/putative inner membrane protein
RA 1,912,078 A→G S167P (TCC→CCC)  htpX ← zinc dependent endoprotease
RA 1,957,020 G→A intergenic (‑13/+12) torZ ← / ← torY trimethylamine N‑oxide reductase 2/cytochrome c quinol dehydrogenase TorY
RA 1,957,999 A→G M45T (ATG→ACG)  torY ← cytochrome c quinol dehydrogenase TorY
RA 1,965,121 T→C G357G (GGA→GGG flhB ← flagellar biosynthesis protein FlhB
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,022,686 T→C L24P (CTA→CCA)  fliQ → flagellar biosynthesis protein FliQ
RA 2,023,730 A→G intergenic (+52/‑238) fliR → / → rcsA flagellar biosynthesis protein FliR/DNA‑binding transcriptional activator RcsA
RA 2,027,131 T→C H296R (CAT→CGT)  dgcQ ← putative diguanylate cyclase DgcQ
RA 2,028,495 A→G V291A (GTG→GCG)  yedI ← conserved inner membrane protein YedI
RA 2,043,483 (G)5→6 noncoding (75/90 nt) serU ← tRNA‑Ser
RA 2,077,169 T→C W20R (TGG→CGG)  cbeA → CP4‑44 prophage; cytoskeleton bundling‑enhancing antitoxin CbeA
RA 2,077,174 G→A G21G (GGG→GGA cbeA → CP4‑44 prophage; cytoskeleton bundling‑enhancing antitoxin CbeA
RA 2,078,709 G→A A45A (GCG→GCA yoeF → CP4‑44 prophage; putative uncharacterized protein YoeF
RA 2,089,275 T→C H64R (CAC→CGC)  yoeB ← ribosome‑dependent mRNA interferase toxin YoeB
RA 2,111,569 T→C E136E (GAA→GAG rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,153,889 C→T intergenic (+108/‑127) sibB → / → mdtA small RNA SibB/multidrug efflux pump membrane fusion protein MdtA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,173,803 T→C pseudogene (476/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,229,182 (G)5→4 intergenic (+117/+256) yohP → / ← dusC putative membrane protein YohP/tRNA‑dihydrouridine(16) synthase
RA 2,272,277 T→C W518R (TGG→CGG)  pdeN → putative c‑di‑GMP phosphodiesterase PdeN
RA 2,302,553 A→G C67C (TGT→TGC napA ← periplasmic nitrate reductase subunit NapA
RA 2,310,285 A→G S41P (TCT→CCT)  ada ← DNA‑binding transcriptional dual regulator/DNA repair protein Ada
RA 2,323,749 T→C G101G (GGT→GGC atoD → acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
RA 2,326,589 A→G T161A (ACC→GCC)  atoB → acetyl‑CoA acetyltransferase
RA 2,330,784 T→C pseudogene (3619/4605 nt) yfaS ← putative macroglobulin family protein, N‑terminal fragment
RA 2,342,454 T→C T572T (ACA→ACG yfaL ← putative autotransporter adhesin YfaL
RA 2,399,675 T→C Y164C (TAC→TGC)  nuoG ← NADH:quinone oxidoreductase subunit G
RA 2,405,158 (A)8→7 intergenic (‑86/+545) nuoA ← / ← lrhA NADH:quinone oxidoreductase subunit A/DNA‑binding transcriptional dual regulator LrhA
RA 2,428,805 G→A F478F (TTC→TTT purF ← amidophosphoribosyltransferase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,453,540 T→C pseudogene (1462/2646 nt) yfcU ← putative outer membrane usher protein YfcU
RA 2,479,931 T→C L244L (TTG→CTG)  dsdA → D‑serine ammonia‑lyase
RA 2,490,509 T→C D481G (GAC→GGC)  oxc ← oxalyl‑CoA decarboxylase
RA 2,502,361 A→G C709R (TGC→CGC)  fryA ← putative PTS multiphosphoryl transfer protein FryA
RA 2,509,057 G→A P124S (CCG→TCG)  glk ← glucokinase
RA 2,511,730 A→C V326G (GTG→GGG)  mntH ← Mn(2(+))/Fe(2(+)): H(+) symporter MntH
RA 2,524,660 A→T H73Q (CAT→CAA xapA ← xanthosine phosphorylase
RA 2,527,795 T→C F284L (TTC→CTC)  yfeH → putative solute:Na(+) symporter
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,581,601 G→A A656T (GCA→ACA)  tktB → transketolase 2
RA 2,591,633 A→G T9T (ACA→ACG dapE → succinyl‑diaminopimelate desuccinylase
RA 2,601,506 C→T S102S (AGC→AGT hyfA → hydrogenase 4 component A
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,602,306 T→C A163A (GCT→GCC hyfB → hydrogenase 4 component B
RA 2,607,878 A→G N321S (AAC→AGC)  hyfF → hydrogenase 4 component F
RA 2,610,630 T→C V156A (GTC→GCC)  hyfH → hydrogenase 4 component H
RA 2,656,200 A→G G53G (GGT→GGC pepB ← aminopeptidase B
RA 2,692,928 C→T A872A (GCG→GCA purL ← phosphoribosylformylglycinamide synthetase
RA 2,709,188 T→C T73A (ACA→GCA)  rseA ← anti‑sigma factor
RA 2,723,976 A→G H417R (CAC→CGC)  pssA → phosphatidylserine synthase
RA 2,726,164 A→G noncoding (25/120 nt) rrfG ← 5S ribosomal RNA
RA 2,734,543 (C)8→10 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,749,819 T→C V349A (GTG→GCG)  yfjD → putative inner membrane protein
RA 2,777,401 T→C V96A (GTA→GCA)  yfjZ → CP4‑57 prophage; putative antitoxin of the YpjF‑YfjZ toxin‑antitoxin system
RA 2,779,192 T→C T1179A (ACC→GCC)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,786,387 G→A intergenic (‑550/‑10) ileY ← / → ygaQ tRNA‑Ile/putative uncharacterized protein YgaQ
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,832,101 T→C V63V (GTA→GTG norR ← DNA‑binding transcriptional dual regulator NorR
RA 2,845,589 T→C T295A (ACC→GCC)  hycE ← formate hydrogenlyase subunit HycE
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,863,999 T→C V136A (GTG→GCG)  ygbL → putative 3‑oxo‑tetronate 4‑phosphate decarboxylase YgbL
RA 2,891,531 T→C E123G (GAA→GGA)  cysJ ← sulfite reductase, flavoprotein subunit
RA 2,906,062 A→C T118P (ACC→CCC)  ygcG → protein YgcG
RA 2,927,714 T→C intergenic (+42/‑515) ppnN → / → sdaC nucleotide 5'‑monophosphate nucleosidase/serine:H(+) symporter SdaC
RA 2,937,272 T→C R563R (CGT→CGC fucI → L‑fucose isomerase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,958,537 A→G G116G (GGT→GGC ptrA ← protease 3
RA 2,988,044 A→G Y170C (TAT→TGT)  yqeH → putative LuxR family transcriptional regulator YqeH
RA 2,999,680 (G)7→9 coding (212/756 nt) ygeR ← LysM domain‑containing putative peptidase lipoprotein YgeR
RA 3,001,304 (G)7→8 coding (960/2259 nt) xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,004,053 A→G A578A (GCT→GCC ygeV ← putative sigma(54)‑dependent transcriptional regulator YgeV
RA 3,011,406 T→C V460A (GTG→GCG)  hyuA → phenylhydantoinase
RA 3,046,876 T→C G722G (GGA→GGG gcvP ← glycine decarboxylase
RA 3,056,324 T→C Y28Y (TAT→TAC fau → putative 5‑formyltetrahydrofolate cyclo‑ligase
RA 3,058,859 T→C Y156C (TAC→TGC)  rpiA ← ribose‑5‑phosphate isomerase A
RA 3,060,042 T→C V97A (GTC→GCC)  argP → DNA‑binding transcriptional dual regulator ArgP
RA 3,063,040 G→T E18D (GAG→GAT argK → methylmalonyl‑CoA mutase‑interacting GTPase YgfD
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,085,503 A→G S137P (TCC→CCC)  speA ← arginine decarboxylase, biosynthetic
RA 3,085,818 C→T A32T (GCC→ACC)  speA ← arginine decarboxylase, biosynthetic
RA 3,089,017 A→G N245S (AAC→AGC)  galP → galactose:H(+) symporter
RA 3,111,797 G→A pseudogene (192/861 nt) yghE ← putative type II secretion system L‑type protein
RA 3,141,483 T→C T503A (ACC→GCC)  hybC ← hydrogenase 2 large subunit
RA 3,142,568 T→C H141R (CAC→CGC)  hybC ← hydrogenase 2 large subunit
RA 3,146,549 G→A R63R (CGC→CGT yghW ← DUF2623 domain‑containing protein YghW
RA 3,192,444 (T)7→8 coding (237/630 nt) yqiJ → putative inner membrane protein
RA 3,208,251 G→A P76P (CCG→CCA ttdT → L‑tartrate:succinate antiporter
RA 3,232,156 C→T S164S (TCC→TCT fadH → 2,4‑dienoyl‑CoA reductase
RA 3,252,476 T→C I58T (ATC→ACC)  yhaH → putative inner membrane protein
RA 3,253,881 (A)5→4 coding (334/897 nt) yhaJ ← DNA‑binding transcriptional activator YhaJ
RA 3,272,461 T→C A97A (GCA→GCG garR ← tartronate semialdehyde reductase
RA 3,289,657 T→C L282L (TTG→CTG)  yraJ → putative fimbrial usher protein YraJ
RA 3,310,823 T→C T116A (ACC→GCC)  pnp ← polynucleotide phosphorylase
RA 3,322,662 (A)7→8 intergenic (‑157/+71) secG ← / ← glmM Sec translocon subunit SecG/phosphoglucosamine mutase
RA 3,324,546 T→C E122E (GAA→GAG folP ← dihydropteroate synthase
RA 3,327,342 G→A A108V (GCA→GTA)  rlmE ← 23S rRNA 2'‑O‑ribose U2552 methyltransferase
RA 3,333,218 A→G R78R (CGT→CGC rplU ← 50S ribosomal subunit protein L21
RA 3,337,189 T→C D23G (GAC→GGC)  mlaB ← intermembrane phospholipid transport system protein MlaB
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,368,963 G→A intergenic (+9/+51) yhcG → / ← yhcH DUF1016 domain‑containing protein YhcG/DUF386 domain‑containing protein YhcH
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,392,260 C→T intergenic (‑232/+198) tldD ← / ← yhdP metalloprotease TldD/AsmA2 domain‑containing protein
RA 3,396,892 T→C T302A (ACC→GCC)  rng ← RNase G
RA 3,457,801 G→A G475G (GGG→GGA gspD → Type II secretion system protein GspD
RA 3,462,961 T→C V13A (GTA→GCA)  gspK → Type II secretion system protein GspK
RA 3,474,354 T→C I67V (ATC→GTC)  rpsL ← 30S ribosomal subunit protein S12
RA 3,484,088 (C)6→7 coding (887/1023 nt) yheT → putative hydrolase YheT
RA 3,489,320 A→G G287G (GGT→GGC argD ← N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase
RA 3,511,233 G→A P64S (CCG→TCG)  yhfY ← conserved protein YhfY
RA 3,541,397 T→C F331L (TTC→CTC)  feoB → Fe(2(+)) transporter FeoB
RA 3,544,403 T→C T148A (ACC→GCC)  bioH ← pimeloyl‑acyl carrier protein methyl ester esterase
RA 3,547,748 G→A G397D (GGC→GAC)  gntT → high‑affinity gluconate transporter
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,563,647 T→C intergenic (+129/+77) glpD → / ← yzgL aerobic glycerol 3‑phosphate dehydrogenase/putative uncharacterized protein YzgL
RA 3,565,249 T→C M445V (ATG→GTG)  glgP ← glycogen phosphorylase
RA 3,629,661 A→C R314R (CGT→CGG yhiI ← putative membrane fusion protein YhiI
RA 3,639,721 A→G intergenic (‑1/‑390) uspB ← / → uspA putative universal stress (ethanol tolerance) protein B/universal stress global stress response regulator
RA 3,664,804 A→G intergenic (‑186/‑60) gadW ← / → gadY DNA‑binding transcriptional dual regulator GadW/small regulatory RNA GadY
RA 3,670,288 T→C L233L (TTG→CTG)  treF → cytoplasmic trehalase
RA 3,673,175 G→A R254R (CGG→CGA yhjC → putative DNA‑binding transcriptional regulator YhjC
RA 3,684,903 T→C N239S (AAC→AGC)  pdeK ← putative c‑di‑GMP phosphodiesterase PdeK
RA 3,708,506 C→T intergenic (‑801/+110) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,728,118 (C)8→9 coding (1254/1455 nt) xylB ← xylulokinase
RA 3,757,729 A→G R33R (CGT→CGC yiaY ← L‑threonine dehydrogenase
RA 3,782,970 G→A A231V (GCG→GTG)  gpsA ← glycerol‑3‑phosphate dehydrogenase
RA 3,784,960 A→T I19N (ATC→AAC)  yibN ← putative sulfurtransferase YibN
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,903,168 G→A H178H (CAC→CAT bglB ← 6‑phospho‑beta‑glucosidase B
RA 3,917,862 G→A A135V (GCC→GTC)  atpG ← ATP synthase F1 complex subunit gamma
RA 3,927,392 A→G T80A (ACC→GCC)  asnA → asparagine synthetase A
RA 3,940,176 A→G V146A (GTG→GCG)  hsrA ← putative transporter HsrA
RA 3,946,671 A→G intergenic (+64/‑29) rrlC → / → rrfC 23S ribosomal RNA/5S ribosomal RNA
RA 3,949,385 A→G G195G (GGT→GGC yifB ← putative magnesium chelatase YifB
RA 3,951,372 A→G pseudogene (813/1645 nt) ilvG → acetolactate synthase II subunit IlvG, N‑terminal fragment (pseudogene)
RA 4,045,212 T→C Y338Y (TAT→TAC yihF → uncharacterized protein YihF
RA 4,045,596 A→G E466E (GAA→GAG yihF → uncharacterized protein YihF
RA 4,103,076 T→C G85G (GGT→GGC yiiM → 2‑amino‑6‑N‑hydroxylaminopurine resistance protein
RA 4,108,324 T→C V258A (GTG→GCG)  pfkA → 6‑phosphofructokinase I
RA 4,117,507 C→T G195D (GGC→GAC)  glpF ← glycerol facilitator
RA 4,153,570 G→A intergenic (‑472/+126) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,178,657 A→G T71A (ACC→GCC)  rplK → 50S ribosomal subunit protein L11
RA 4,258,838 A→G Q366R (CAG→CGG)  dinF → DNA damage‑inducible protein F
RA 4,285,630 T→C N581S (AAC→AGC)  acs ← acetyl‑CoA synthetase (AMP‑forming)
RA 4,292,836 A→G Y442C (TAC→TGC)  nrfE → putative cytochrome c‑type biogenesis protein NrfE
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,299,189 G→A P60S (CCG→TCG)  fdhF ← formate dehydrogenase H
RA 4,306,256 A→G G114G (GGT→GGC yjcS ← alkyl sulfatase
RA 4,353,616 A→G L368L (TTG→CTG)  lysU ← lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
RA 4,359,120 A→G G304G (GGT→GGC cadB ← lysine:cadaverine antiporter
RA 4,370,118 C→T G99S (GGT→AGT)  yjeH ← L‑methionine/branched chain amino acid exporter
RA 4,376,294 A→G intergenic (+29/‑23) efp → / → ecnA protein chain elongation factor EF‑P/entericidin A lipoprotein, antidote to entericidin B
RA 4,377,330 G→A C131C (TGC→TGT blc ← outer membrane lipoprotein Blc
RA 4,407,624 A→G Y324C (TAC→TGC)  rnr → RNase R
RA 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA 2'‑O‑ribose G2251 methyltransferase/conserved protein YjfI
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,472,524 A→G C4R (TGT→CGT)  pyrL ← pyrBI operon leader peptide
RA 4,483,608 A→G V77A (GTC→GCC)  valS ← valine‑‑tRNA ligase
RA 4,486,769 T→C R184R (CGT→CGC lptF → lipopolysaccharide transport system protein LptF
RA 4,487,609 A→G T98A (ACC→GCC)  lptG → lipopolysaccharide transport system protein LptG
RA 4,509,831 G→A pseudogene (28/330 nt) yjhV → KpLE2 phage‑like element; putative uncharacterized protein YjhV
RA 4,523,851 C→T A82T (GCG→ACG)  yjhG ← KpLE2 phage‑like element; D‑xylonate dehydratase
RA 4,527,408 T→C T44A (ACG→GCG)  sgcE ← KpLE2 phage‑like element; putative epimerase SgcE
RA 4,537,169 A→G D142D (GAT→GAC nanS ← N‑acetyl‑9‑O‑acetylneuraminate esterase
RA 4,543,190 A→G T26A (ACG→GCG)  fimA → type 1 fimbriae major subunit
RA 4,560,459 (C)5→4 coding (222/684 nt) yjiH ← Gate family protein YjiH
RA 4,565,730 T→C T41A (ACC→GCC)  yjiM ← putative dehydratase subunit
RA 4,568,337 A→G G61G (GGT→GGC mdtM ← multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 4,586,594 (G)6→7 coding (168/3513 nt) hsdR ← type I restriction enzyme EcoKI endonuclease component
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 433032 433127 96 6 [5] [5] 6 nrdR NrdR transcriptional repressor
* * ÷ NC_000913 1196276 1211423 15148 7 [5] [5] 6 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423794–3424230 3424523–3424240 11–730 6 [5] [2] 6 [rrlD] [rrlD]
* * ÷ NC_000913 3945908–3946225 3946500–3946231 7–593 6 [3] [5] 8 rrlC 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374922 =NA (NA)4 (0.140) 3/204 3.0 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377430 = NA (NA)intergenic (+119/+105) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)4 (0.150) 4/192 2.5 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 12994980 (0.000)43 (1.120) 34/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =75 (1.890)73 (1.860) 47/276 0.0 65.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.080)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)60 (1.510) 41/278 0.1 96.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA