breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,875 A→G V513V (GTA→GTG thrA → fused aspartate kinase/homoserine dehydrogenase 1
RA 7,390 C→T L190L (CTG→CTA yaaJ ← putative transporter YaaJ
RA 70,424 A→G Y13C (TAC→TGC)  araC → DNA‑binding transcriptional dual regulator AraC
RA 80,625 A→G R80R (CGT→CGC leuC ← 3‑isopropylmalate dehydratase subunit LeuC
RA 112,873 A→G V116A (GTA→GCA)  coaE ← dephospho‑CoA kinase
RA 114,907 G→A T273I (ACA→ATA)  hofC ← inner membrane protein HofC
RA 120,112 A→G T278A (ACG→GCG)  ampE → protein AmpE
RA 140,924 C→T G101D (GGC→GAC)  gcd ← quinoprotein glucose dehydrogenase
RA 189,159 A→G G116G (GGT→GGC map ← methionine aminopeptidase
RA 202,893 G→A G112S (GGC→AGC)  lpxA → UDP‑N‑acetylglucosamine acyltransferase
RA 205,353 G→A L76L (CTG→CTA dnaE → DNA polymerase III subunit alpha
RA 219,654 A→G G114G (GGT→GGC rcsF ← outer membrane lipoprotein RcsF
RA 234,139 (A)7→8 coding (644/756 nt) gloB ← hydroxyacylglutathione hydrolase GloB
RA 240,779 G→A S146N (AGC→AAC)  ivy → periplasmic chaperone, inhibitor of vertebrate C‑type lysozyme
RA 256,049 C→T P25S (CCT→TCT)  gpt → xanthine‑guanine phsophoribosyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 272,498 T→C intergenic (+308/‑82) insN → / → eyeA CP4‑6 prophage; IS911A regulator fragment/CP4‑6 prophage; small RNA EyeA
RA 285,647 (G)6→7 coding (253/1383 nt) yagG → putative D‑xylonate transporter YagG
RA 291,343 C→T A2T (GCT→ACT)  insA‑3 ← IS1 protein InsA
RA 294,752 (T)7→6 coding (48/855 nt) yagM ← CP4‑6 prophage; protein YagM
RA 297,827 T→C E300E (GAA→GAG paoD ← molybdenum cofactor insertion chaperone for PaoABC
RA 310,057 (G)6→7 intergenic (‑31/+27) ecpB ← / ← ecpA putative fimbrial chaperone EcpB/common pilus major subunit
RA 316,612 A→G pseudogene (525/687 nt) ykgA ← putative DNA‑binding transcriptional regulator YkgA
RA 329,082 C→T E85K (GAG→AAG)  betI ← DNA‑binding transcriptional repressor BetI
RA 330,537 T→C F359L (TTT→CTT)  betT → choline:H(+) symporter
RA 334,301 T→C T45A (ACG→GCG)  yahB ← putative LysR‑type DNA‑binding transcriptional regulator YahB
RA 354,768 A→G intergenic (+176/‑154) prpE → / → codB propionyl‑CoA synthetase/cytosine transporter
RA 364,824 G→A T494T (ACC→ACT lacZ ← beta‑galactosidase
RA 371,379 T→C G47G (GGT→GGC mhpC → 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
RA 379,940 A→G intergenic (‑59/+129) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 380,021 (G)10→8 intergenic (‑140/+47) frmR ← / ← yaiO DNA‑binding transcriptional repressor FrmR/outer membrane protein YaiO
RA 391,703 (G)6→7 pseudogene (1453/1458 nt) yaiT → putative autotransporter YaiT
RA 421,903 C→T G306G (GGC→GGT proY → putative transporter ProY
RA 422,427 (C)5→7 intergenic (+68/‑91) proY → / → malZ putative transporter ProY/maltodextrin glucosidase
RA 426,301 C→T L55L (CTC→CTT tgt → tRNA‑guanine transglycosylase
RA 466,851 G→A G154G (GGC→GGT ybaE ← putative protein YbaE
RA 474,447 A→G F239L (TTT→CTT)  tesB ← acyl‑CoA thioesterase II
RA 503,439 A→G intergenic (‑201/+37) ybaL ← / ← fsr putative transporter YbaL/fosmidomycin efflux pump
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 530,394 T→C Y278C (TAT→TGT)  selU ← tRNA 2‑selenouridine synthase
RA 592,150 T→G K343N (AAA→AAC nfrB ← bacteriophage N4 receptor, inner membrane subunit
RA 595,667 A→G D23G (GAT→GGT)  cusC → copper/silver export system outer membrane channel
RA 627,410 C→T I447I (ATC→ATT entE → 2,3‑dihydroxybenzoate‑AMP ligase
RA 640,890 C→T H380Y (CAC→TAC)  ahpF → alkyl hydroperoxide reductase, AhpF component
RA 649,194 A→G G126G (GGT→GGC citF ← citrate lyase alpha subunit
RA 657,549 (T)8→7 intergenic (+48/+6) cspE → / ← crcB transcription antiterminator and regulator of RNA stability/F(‑) channel
RA 660,412 T→C intergenic (‑196/+13) lipA ← / ← ybeF lipoyl synthase/putative LysR‑type DNA‑binding transcriptional regulator YbeF
RA 684,415 T→C intergenic (‑3/+115) rihA ← / ← gltL pyrimidine‑specific ribonucleoside hydrolase RihA/glutamate/aspartate ABC transporter ATP binding subunit
RA 691,798 T→G intergenic (‑14/+76) ybeX ← / ← ybeY CorC‑HlyC family protein YbeX/endoribonuclease YbeY
RA 692,306 A→G C12C (TGT→TGC ybeY ← endoribonuclease YbeY
RA 710,005 A→G V72V (GTA→GTG chiQ → lipoprotein ChiQ
RA 715,004 A→G T483A (ACT→GCT)  pgm → phosphoglucomutase
RA 722,788 A→G F543L (TTT→CTT)  kdpD ← sensory histidine kinase KdpD
RA 742,314 (C)5→4 coding (243/1482 nt) dtpD ← dipeptide:H(+) symporter DtpD
RA 771,858 C→T A134V (GCG→GTG)  cydA → cytochrome bd‑I ubiquinol oxidase subunit I
RA 784,802 T→C T8A (ACG→GCG)  zitB ← Zn(2(+))/Cd(2(+))/Ni(2(+))/Cu(2(+)) exporter
RA 814,014 C→T V163V (GTC→GTT uvrB → excision nuclease subunit B
RA 829,181 A→G Y264Y (TAT→TAC ybhG ← HlyD_D23 family protein YbhG
RA 838,323 G→A P45L (CCC→CTC)  ybiI ← zinc finger domain‑containing protein YbiI
RA 858,438 T→C A208A (GCA→GCG ybiU ← DUF1479 domain‑containing protein YbiU
RA 862,465 T→A I48F (ATC→TTC)  ybiW ← putative pyruvate formate lyase
RA 886,100 T→C D11G (GAT→GGT)  ybjI ← 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 915,319 (A)7→8 intergenic (‑462/+33) lysO ← / ← aqpZ L‑lysine exporter/water channel AqpZ
RA 929,457 A→G N502N (AAT→AAC cydD ← glutathione/L‑cysteine ABC exporter subunit CydD
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 934,388 C→T P389S (CCT→TCT)  ftsK → cell division DNA translocase FtsK
RA 943,761 T→C Y116Y (TAT→TAC dmsB → dimethyl sulfoxide reductase subunit B
RA 1,010,725 A→G L254L (TTA→TTG uup → ATP‑binding protein with possible role in replication
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,016,702 A→G V533A (GTG→GCG)  ycbZ ← putative ATP‑dependent protease YcbZ
RA 1,045,000 C→T T268T (ACG→ACA gfcE ← putative exopolysaccharide export lipoprotein GfcE
RA 1,048,903 T→C T144A (ACT→GCT)  gfcB ← lipoprotein GfcB
RA 1,054,270 C→T A637T (GCC→ACC)  torS ← sensory histidine kinase TorS
RA 1,057,108 A→G E283G (GAA→GGA)  torT → periplasmic trimethylamine‑N‑oxide binding protein
RA 1,087,643 A→G A68A (GCT→GCC pgaC ← poly‑N‑acetyl‑D‑glucosamine synthase subunit PgaC
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,206,680 A→G pseudogene (550/792 nt) jayE → e14 prophage; putative protein JayE
RA 1,212,088 (C)8→9 intergenic (‑85/+172) iraM ← / ← ymgK anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
RA 1,215,983 A→G E65E (GAA→GAG ycgZ → putative two‑component system connector protein YcgZ
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,222,729 A→G V87A (GTT→GCT)  ymgG ← PF13488 family protein YmgG
RA 1,227,593 (G)6→7 intergenic (‑121/‑88) pliG ← / → ycgL inhibitor of g‑type lysozyme/PF05166 family protein YcgL
RA 1,237,673 (G)5→6 coding (103/1299 nt) dadA → D‑amino acid dehydrogenase
RA 1,266,239 A→G D49G (GAC→GGC)  prmC → protein‑(glutamine‑N(5)) methyltransferase
RA 1,269,425 (C)5→4 intergenic (+36/+278) rdlA → / ← ldrB putative antisense RNA regulator of LdrA/small toxic polypeptide LdrB
RA 1,296,183 A→G C647R (TGT→CGT)  adhE ← aldehyde‑alcohol dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,326,396 A→G T551A (ACA→GCA)  yciQ → DUF2207 domain‑containing protein YciQ
RA 1,331,358 A→G D104G (GAC→GGC)  topA → DNA topoisomerase 1
RA 1,336,451 A→G T207T (ACA→ACG acnA → aconitate hydratase 1
RA 1,351,196 (G)7→6 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,357,671 T→G intergenic (‑3/+131) ymjA ← / ← puuP DUF2543 domain‑containing protein YmjA/putrescine:H(+) symporter PuuP
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
RA 1,417,546 (T)8→7 coding (218/276 nt) racC ← Rac prophage; protein RacC
RA 1,420,131 T→C Q37Q (CAA→CAG racR ← Rac prophage; DNA‑binding transcriptional repressor RacR
RA 1,423,575 A→G pseudogene (231/300 nt)
N59S (AAC→AGC) 
rzpR →
rzoR →
Rac prophage; putative endopeptidase RzpR
Rac prophage; putative lipoprotein
RA 1,434,347 A→G A20A (GCT→GCC ynaM ← Rac prophage; protein YnaM
RA 1,440,632 T→C T51T (ACA→ACG ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,485,031 T→C G657G (GGT→GGC hrpA → ATP‑dependent RNA helicase HrpA
RA 1,488,314 A→G N28S (AAC→AGC)  aldA → aldehyde dehydrogenase A
RA 1,491,291 A→G V130V (GTA→GTG cybB → cytochrome b561
RA 1,535,988 C→T A210A (GCG→GCA narV ← nitrate reductase Z subunit gamma
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,564,808 T→C H309R (CAC→CGC)  dosP ← oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
RA 1,587,901 G→A I132I (ATC→ATT ydeR ← putative fimbrial protein YdeR
RA 1,591,925 T→C R84R (AGA→AGG hipA ← serine/threonine kinase HipA
RA 1,594,132 (C)8→9 pseudogene (3955/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,596,091 A→G pseudogene (1996/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,597,313 (C)7→9 pseudogene (774/5422 nt) yneO ← AIDA‑I family autotransporter YneO
RA 1,601,771 A→G E94E (GAA→GAG lsrA → Autoinducer‑2 ABC transporter ATP binding subunit
RA 1,632,303 (T)6→5 intergenic (‑18/‑587) ydfJ ← / → ynfT putative transporter YdfJ/Qin prophage; protein YnfT
RA 1,671,708 Δ1 bp intergenic (+24/‑69) asr → / → ydgU acid shock protein/uncharacterized protein YdgU
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,705,751 (T)6→5 intergenic (+61/‑16) ydgK → / → rsxA conserved inner membrane protein YdgK/SoxR [2Fe‑2S] reducing system protein RsxA
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,714,584 A→G M70V (ATG→GTG)  gstA → glutathione S‑transferase GstA
RA 1,735,284 T→C intergenic (+34/‑94) mepH → / → sodB peptidoglycan DD‑endopeptidase MepH/superoxide dismutase (Fe)
RA 1,738,197 T→C G118G (GGT→GGC purR → DNA‑binding transcriptional repressor PurR
RA 1,784,065 G→A V363V (GTG→GTA fadK → short chain acyl‑CoA synthetase
RA 1,799,072 T→C intergenic (‑130/+154) pheS ← / ← pheM phenylalanine‑‑tRNA ligase subunit alpha/pheST‑ihfA operon leader peptide
MC JC 1,834,817 (ATG)4→3 coding (10‑12/708 nt) ydjZ → DedA family protein YdjZ
RA 1,849,930 T→C V365A (GTG→GCG)  sppA → protease IV, a signal peptide peptidase
RA 1,870,094 T→C R380R (CGT→CGC yeaH → DUF444 domain‑containing protein YeaH
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,892,882 (C)6→7 coding (354/1911 nt) yoaA ← putative 5' to 3' DNA helicase implicated in DNA repair
RA 1,926,868 G→A R658C (CGC→TGC)  ptrB ← oligopeptidase B
RA 1,928,699 A→G G47G (GGT→GGC ptrB ← oligopeptidase B
RA 1,928,733 A→G V36A (GTC→GCC)  ptrB ← oligopeptidase B
RA 1,945,866 T→C E37E (GAA→GAG ruvA ← Holliday junction branch migration complex subunit RuvA
RA 1,947,257 A→C D40E (GAT→GAG ruvC ← crossover junction endodeoxyribonuclease RuvC
RA 1,955,459 T→C T517A (ACC→GCC)  torZ ← trimethylamine N‑oxide reductase 2
RA 1,956,871 G→A A46V (GCG→GTG)  torZ ← trimethylamine N‑oxide reductase 2
RA 1,974,960 G→A A122V (GCC→GTC)  cheA ← chemotaxis protein CheA
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,986,976 T→C F18L (TTT→CTT)  ftnB → putative ferritin‑like protein
RA 2,015,075 T→C F66L (TTT→CTT)  fliG → flagellar motor switch protein FliG
RA 2,017,886 T→C S445P (TCT→CCT)  fliI → flagellum‑specific ATP synthase FliI
RA 2,024,436 C→T Q157* (CAA→TAA)  rcsA → DNA‑binding transcriptional activator RcsA
RA 2,095,695 T→C C191R (TGC→CGC)  hisA → 1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino]imidazole‑4‑ carboxamide isomerase
RA 2,096,038 G→A W65* (TGG→TGA hisF → imidazole glycerol phosphate synthase subunit HisF
RA 2,096,351 G→A G170S (GGT→AGT)  hisF → imidazole glycerol phosphate synthase subunit HisF
RA 2,124,085 T→C T146A (ACC→GCC)  cpsB ← mannose‑1‑phosphate guanylyltransferase
RA 2,127,723 T→C T198A (ACC→GCC)  gmd ← GDP‑mannose 4,6‑dehydratase
RA 2,135,234 G→A T140I (ACC→ATC)  wzc ← protein‑tyrosine kinase Wzc
RA 2,146,949 T→C I198V (ATA→GTA)  alkA ← DNA‑3‑methyladenine glycosylase 2
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,187,323 (G)5→6 intergenic (+25/+57) rcnB → / ← yehA periplasmic protein involved in nickel/cobalt export/putative fimbrial adhesin YehA
RA 2,197,183 T→C V237A (GTA→GCA)  yehF → putative protein YehF
RA 2,206,448 A→G M252V (ATG→GTG)  yehM → putative YehN
RA 2,224,365 A→G V169A (GTG→GCG)  pbpG ← peptidoglycan DD‑endopeptidase PbpG
RA 2,233,659 C→T F20F (TTC→TTT yeiS → DUF2542 domain‑containing protein YeiS
RA 2,234,448 C→T A139V (GCC→GTC)  preT → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
RA 2,291,359 (T)5→6 coding (1052/1053 nt) ccmH ← holocytochrome c synthetase ‑ thiol:disulfide oxidoreductase CcmH
RA 2,297,738 T→C K174K (AAA→AAG napC ← periplasmic nitrate reductase cytochrome c protein
RA 2,301,107 T→C V549V (GTA→GTG napA ← periplasmic nitrate reductase subunit NapA
RA 2,312,343 T→C Y136C (TAC→TGC)  ompC ← outer membrane porin C
RA 2,362,013 T→C T67A (ACC→GCC)  yfaX ← putative DNA‑binding transcriptional regulator YfaX
RA 2,362,203 T→C E3E (GAA→GAG yfaX ← putative DNA‑binding transcriptional regulator YfaX
RA 2,378,828 G→A P144P (CCC→CCT menD ← 2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase
RA 2,402,588 T→C T420A (ACC→GCC)  nuoC ← NADH:quinone oxidoreductase subunit CD
RA 2,415,078 T→C V111A (GTC→GCC)  pta → phosphate acetyltransferase
RA 2,432,681 G→A R88C (CGT→TGT)  folC ← bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
RA 2,442,660 (C)5→6 coding (897/2007 nt) mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming methyltransferase and FAD‑dependent demodification enzyme
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,456,619 T→C D65G (GAT→GGT)  sixA ← phosphohistidine phosphatase
RA 2,462,324 T→C F340S (TTC→TCC)  fadL → long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor
RA 2,462,889 (C)9→8 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,464,242 (T)6→7 intergenic (+172/+10) yfdF → / ← mlaA protein YfdF/intermembrane phospholipid transport system ‑ outer membrane lipoprotein MlaA
RA 2,504,414 T→C E24E (GAA→GAG fryA ← putative PTS multiphosphoryl transfer protein FryA
RA 2,510,853 T→C R408R (CGT→CGC yfeO → putative transport protein YfeO
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,556,929 T→C V123V (GTA→GTG eutC ← ethanolamine ammonia‑lyase subunit beta
RA 2,570,274 T→C T22A (ACG→GCG)  eutJ ← putative chaperonin, ethanolamine utilization protein
RA 2,602,017 (C)7→9 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,602,375 +G coding (558/2019 nt) hyfB → hydrogenase 4 component B
RA 2,638,026 (C)6→5 coding (627/1179 nt) bamB ← outer membrane protein assembly factor BamB
RA 2,638,481 T→C N58D (AAC→GAC)  bamB ← outer membrane protein assembly factor BamB
RA 2,662,353 A→G intergenic (‑222/+230) iscR ← / ← trmJ DNA‑binding transcriptional dual regulator IscR/tRNA Cm32/Um32 methyltransferase
RA 2,670,928 T→C C7C (TGT→TGC hcaC → putative 3‑phenylpropionate/cinnamate dioxygenase ferredoxin subunit
RA 2,710,742 T→C I108T (ATT→ACT)  nadB → L‑aspartate oxidase
RA 2,723,976 A→G H417R (CAC→CGC)  pssA → phosphatidylserine synthase
RA 2,731,822 T→C K784K (AAA→AAG clpB ← ClpB chaperone
RA 2,734,543 (C)8→9 coding (492/732 nt) pgeF ← polyphenol oxidase YfiH
RA 2,740,857 T→C K98K (AAA→AAG aroF ← 3‑deoxy‑7‑phosphoheptulonate synthase, Tyr‑sensitive
RA 2,765,794 (C)7→6 intergenic (‑18/‑124) yfjM ← / → rnlA CP4‑57 prophage; protein YfjM/CP4‑57 prophage; RNase LS, toxin of the RnlAB toxin‑antitoxin system
RA 2,767,085 G→A T34T (ACG→ACA rnlB → CP4‑57 prophage; antitoxin RnlB
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,867,926 (C)6→7 coding (828/1140 nt) nlpD ← murein hydrolase activator NlpD
RA 2,877,685 T→C intergenic (+67/+884) iap → / ← cas2 alkaline phosphatase isozyme conversion protein/CRISPR‑associated endoribonuclease Cas2
RA 2,878,876 A→G D299D (GAT→GAC cas1 ← multifunctional nuclease Cas1
RA 2,892,952 T→C V99A (GTA→GCA)  ygcN → putative oxidoreductase with FAD/NAD(P)‑binding domain
RA 2,901,848 (C)5→6 coding (1257/1278 nt) yqcE → putative transport protein YqcE
RA 2,903,363 T→C S490P (TCG→CCG)  ygcE → putative sugar kinase YgcE
RA 2,909,569 C→T G33D (GGC→GAC)  pyrG ← CTP synthetase
RA 2,910,165 T→C D174G (GAC→GGC)  mazG ← nucleoside triphosphate pyrophosphohydrolase
RA 2,920,251 T→C N166D (AAT→GAT)  gudX ← glucarate dehydratase‑related protein
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,955,096 T→C E303G (GAA→GGA)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,955,841 T→C T55A (ACC→GCC)  recB ← exodeoxyribonuclease V subunit RecB
RA 2,970,436 T→C I6T (ATA→ACA)  ygdQ → UPF0053 inner membrane protein YgdQ
RA 2,973,182 T→C L227L (TTA→TTG lplT ← lysophospholipid transporter
RA 2,973,904 A→G F704S (TTT→TCT)  aas ← acyltransferase
RA 2,977,018 T→C G140G (GGT→GGC galR → DNA‑binding transcriptional dual regulator GalR
RA 2,989,323 (A)8→7 coding (20/483 nt) yqeJ → protein YqeJ
RA 3,004,535 C→T A418T (GCC→ACC)  ygeV ← putative sigma(54)‑dependent transcriptional regulator YgeV
RA 3,006,380 A→G D40G (GAC→GGC)  ygeW → putative carbamoyltransferase YgeW
RA 3,026,179 G→A A138A (GCG→GCA guaD → guanine deaminase
RA 3,038,005 T→C G34G (GGA→GGG recJ ← ssDNA‑specific exonuclease RecJ
RA 3,043,737 G→A G26S (GGC→AGC)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,045,063 T→C Y468H (TAC→CAC)  bglA → 6‑phospho‑beta‑glucosidase A
RA 3,057,643 T→C K256K (AAA→AAG serA ← phosphoglycerate dehydrogenase
RA 3,066,127 C→T A442A (GCC→GCT scpC → propionyl‑CoA:succinate CoA transferase
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,068,406 T→C S135G (AGC→GGC)  argO ← L‑arginine exporter
RA 3,107,477 T→C D560G (GAT→GGT)  speC ← ornithine decarboxylase, biosynthetic
RA 3,122,917 T→C T297A (ACT→GCT)  glcB ← malate synthase G
RA 3,154,600 T→C T207A (ACT→GCT)  yqhC ← DNA‑binding transcriptional activator YqhC
RA 3,207,655 C→T G95G (GGC→GGT ttdB → L(+)‑tartrate dehydratase subunit beta
RA 3,230,399 C→T G504G (GGC→GGT ygjK → glycoside hydrolase
RA 3,266,958 C→T P36P (CCG→CCA tdcA ← DNA‑binding transcriptional activator TdcA
RA 3,327,834 A→G A15A (GCA→GCG yhbY → ribosome assembly factor YhbY
RA 3,329,249 T→C V96A (GTA→GCA)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,348,279 (C)6→7 intergenic (+41/‑173) npr → / → yrbL phosphorelay protein Npr/protein kinase‑like domain‑containing protein YrbL
RA 3,350,184 (C)7→8 coding (276/654 nt) elbB ← low activity glyoxalase ElbB
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,354,811 G→A V29V (GTG→GTA gltB → glutamate synthase subunit GltB
RA 3,360,574 T→C G459G (GGT→GGC gltD → glutamate synthase subunit GltD
RA 3,367,500 T→C V192A (GTA→GCA)  yhcF → DUF1120 domain‑containing protein YhcF
RA 3,382,394 T→C G65G (GGT→GGC degS → serine endoprotease
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,402,255 T→C T360A (ACC→GCC)  csrD ← regulator of CsrB and CsrC decay
RA 3,423,701 A→G noncoding (87/120 nt) rrfD ← 5S ribosomal RNA
RA 3,494,433 C→T C141C (TGC→TGT nirB → nitrite reductase catalytic subunit NirB
RA 3,504,971 C→T V350V (GTG→GTA yhfS ← putative aminotransferase YhfS
RA 3,521,245 C→T E69K (GAG→AAG)  hofO ← DNA utilization protein HofO
RA 3,524,518 T→C F550L (TTC→CTC)  mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,569,795 T→C T509A (ACG→GCG)  glgX ← limit dextrin alpha‑1,6‑glucohydrolase
RA 3,586,429 T→C N132S (AAC→AGC)  ggt ← glutathione hydrolase proenzyme
RA 3,601,749 A→G V113A (GTT→GCT)  ftsX ← cell division protein FtsX
RA 3,611,879 A→G Q5Q (CAA→CAG yhhT → putative transporter YhhT
RA 3,648,289 A→G intergenic (+143/‑5) agrA → / → agrB small RNA AgrA/small RNA AgrB
RA 3,651,664 (A)7→6 pseudogene (374/746 nt) yhiS → putative uncharacterized protein YhiS
RA 3,667,921 T→C D423G (GAC→GGC)  ccp ← cytochrome c peroxidase
RA 3,690,189 A→G W25R (TGG→CGG)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,731,244 A→G E38E (GAA→GAG xylF → xylose ABC transporter periplasmic binding protein
RA 3,763,972 A→G N597S (AAC→AGC)  rhsA → rhs element protein RhsA
RA 3,765,830 G→A R1216R (CGG→CGA rhsA → rhs element protein RhsA
RA 3,765,854 G→A G1224G (GGG→GGA rhsA → rhs element protein RhsA
RA 3,770,236 (C)6→8 intergenic (+556/+7) yibV → / ← yibH PF15596 family protein YibV/inner membrane protein YibH
RA 3,773,042 T→C G254G (GGT→GGC mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,852,262 G→A Q175* (CAA→TAA)  ilvB ← acetohydroxy acid synthase I subunit IlvB
RA 3,854,735 A→G T272A (ACG→GCG)  emrD → multidrug efflux pump EmrD
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,870,024 T→C G216G (GGT→GGC cbrA → colicin M resistance protein
RA 3,876,744 T→C N70S (AAC→AGC)  yidA ← sugar phosphatase YidA
RA 3,917,313 A→G D21D (GAT→GAC atpD ← ATP synthase F1 complex subunit beta
RA 3,924,387 T→C D416G (GAC→GGC)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 3,931,868 T→C L185L (TTA→CTA)  kup → K(+):H(+) symporter Kup
RA 3,939,901 A→G L238L (TTA→CTA)  hsrA ← putative transporter HsrA
RA 3,941,955 (G)5→6 noncoding (148/1542 nt) rrsC → 16S ribosomal RNA
RA 3,950,476 (T)8→7 intergenic (+56/‑31) ilvL → / → ilvX ilvXGMEDA operon leader peptide/uncharacterized protein IlvX
RA 3,969,180 (T)6→7 coding (150/1047 nt) wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,983,835 A→G D293G (GAC→GGC)  aslB → putative anaerobic sulfatase maturation enzyme AslB
RA 3,994,386 T→A intergenic (+51/‑136) yzcX → / → yifL protein YzcX/putative lipoprotein YifL
RA 4,050,225 A→G L181L (TTG→CTG)  yihA ← GTP‑binding protein YihA
RA 4,058,537 A→G D44G (GAC→GGC)  bipA → ribosome‑dependent GTPase, ribosome assembly factor
RA 4,060,280 (T)9→8 intergenic (+50/‑167) bipA → / → yihL ribosome‑dependent GTPase, ribosome assembly factor/putative transcriptional regulator YihL
RA 4,094,683 C→T intergenic (‑411/+40) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,094,691 G→T intergenic (‑419/+32) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,094,700 G→A intergenic (‑428/+23) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,094,703 C→T intergenic (‑431/+20) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,094,705 C→T intergenic (‑433/+18) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,128,747 C→T P26S (CCG→TCG)  metB → O‑succinylhomoserine(thiol)‑lyase/O‑ succinylhomoserine lyase
RA 4,155,010 A→G T4A (ACG→GCG)  argC → N‑acetylglutamylphosphate reductase
RA 4,168,571 G→A intergenic (+124/‑70) gltT → / → rrlB tRNA‑Glu/23S ribosomal RNA
RA 4,217,424 T→C Y106H (TAT→CAT)  aceA → isocitrate lyase
RA 4,219,263 (T)5→4 coding (668/1737 nt) aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,250,878 A→G pseudogene (176/1581 nt) yjbI → pentapeptide repeats‑containing protein YjbI
RA 4,263,602 C→T V210V (GTG→GTA qorA ← putative quinone oxidoreductase 1
RA 4,269,672 (A)7→6 coding (259/714 nt) aphA → acid phosphatase/phosphotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,305,427 A→G Y391H (TAC→CAC)  yjcS ← alkyl sulfatase
RA 4,343,821 (T)7→6 coding (453/1422 nt) melB → melibiose:H(+)/Na(+)/Li(+) symporter
RA 4,348,470 A→G V92A (GTC→GCC)  dcuB ← anaerobic C4‑dicarboxylate transporter DcuB
RA 4,360,830 A→G S369P (TCC→CCC)  cadC ← DNA‑binding transcriptional activator CadC
RA 4,363,323 T→C intergenic (‑15/+22) yjdC ← / ← dsbD putative DNA‑binding transcriptional regulator YjdC/protein disulfide oxidoreductase ‑ DsbDreduced
RA 4,384,173 C→T T112I (ACA→ATA)  yjeM → putative transporter YjeM
RA 4,394,870 (G)5→4 coding (805/1548 nt) nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase
RA 4,403,092 T→C V352A (GTT→GCT)  hflK → regulator of FtsH protease
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,446,347 A→G T746A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,449,311 C→T L114L (CTG→CTA ppa ← inorganic pyrophosphatase
RA 4,467,292 A→G intergenic (‑46/‑139) treR ← / → mgtL DNA‑binding transcriptional repressor TreR/leader peptide MgtL
RA 4,482,396 A→G L481P (CTG→CCG)  valS ← valine‑‑tRNA ligase
RA 4,500,982 (C)5→6 intergenic (‑191/‑278) yjgX ← / → yjgZ KpLE2 phage‑like element; putative phosphoethanolamine transferase YjgX, N‑terminal fragment/KpLE2 phage‑like element; uncharacterized protein YjgZ
RA 4,511,541 (G)5→6 coding (874/957 nt) fecD ← ferric citrate ABC transporter membrane subunit FecD
RA 4,516,073 A→G V202A (GTC→GCC)  fecA ← ferric citrate outer membrane transporter
RA 4,517,951 (C)6→7 coding (285/522 nt) fecI ← RNA polymerase sigma factor FecI
RA 4,524,555 A→G R152R (CGT→CGC yjhH ← KpLE2 phage‑like element; putative 2‑dehydro‑3‑deoxy‑D‑pentonate aldolase
RA 4,551,005 G→A R98C (CGC→TGC)  gntP ← fructuronate transporter
RA 4,558,867 +A coding (660/1173 nt) iadA ← isoaspartyl dipeptidase
RA 4,559,164 A→G R121R (CGT→CGC iadA ← isoaspartyl dipeptidase
RA 4,568,725 C→T intergenic (‑206/‑273) mdtM ← / → rpnD multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter/truncated recombination‑promoting nuclease‑like protein RpnD
RA 4,582,379 G→A L224L (CTG→TTG)  hsdM ← type I restriction enzyme EcoKI methyltransferase component
RA 4,585,372 G→A Q464* (CAA→TAA)  hsdR ← type I restriction enzyme EcoKI endonuclease component
RA 4,599,590 A→G intergenic (‑149/+105) opgB ← / ← yjjA phosphoglycerol transferase I/DUF2501 domain‑containing protein YjjA
RA 4,606,215 (G)8→10 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,622,890 C→T S374S (AGC→AGT yjjJ → toxin YjjJ
RA 4,634,532 T→C D260G (GAT→GGT)  rob ← DNA‑binding transcriptional dual regulator Rob
RA 4,636,119 C→T P38L (CCG→CTG)  creB → DNA‑binding transcriptional regulator CreB

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1433355 1433529–1433373 19–175 18 [9] [9] 12 pinR Rac prophage; putative site‑specific recombinase
* * ÷ NC_000913 1433778–1434069 1434256–1434079 11–479 13 [10] [10] 13 ynaE ynaE
* * ÷ NC_000913 1633339–1633616 1633798–1633630 15–460 13 [9] [10] 11 [pinQ] [pinQ]
* * ÷ NC_000913 1634052 1634907–1634643 592–856 11 [8] [10] 11 [pinQ]–[ydfN] [pinQ],tfaQ,[ydfN]
* * ÷ NC_000913 3423766–3424233 3424530–3424238 6–765 11 [10] [10] 11 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3619361–3621079 3622178–3621086 8–2818 11 [10] [9] 13 rhsB rhs element protein RhsB
* * ÷ NC_000913 3764173–3765288 3765789–3765672 385–1617 11 [8] [10] 13 rhsA rhs element protein RhsA
* * ÷ NC_000913 3944154–3946508 3946508 1–2355 12 [10] [8] 13 rrlC 23S ribosomal RNA
* * ÷ NC_000913 4037963–4038688 4039280–4038730 43–1318 11 [10] [9] 12 rrlA 23S ribosomal RNA
* * ÷ NC_000913 4094415 4094673 259 11 [9] [9] 11 rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =86 (1.690)69 (1.360) 42/274 0.1 61.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)52 (1.020) 46/276 0.1 92.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.080)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 454269033 (0.650)12 (0.250) 11/258 2.3 21.3% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 58 (1.220)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit